Melatonin's Protective Role Against Bisphenol F and S-Induced Skeletal Damage: A Morphometric and Histological Study in Rat.

This study aimed to evaluate the potential effects of Bisphenol F and S exposure on the skeletal structures of Sprague-Dawley rats. Given the increasing concern about the potential endocrine-disrupting effects of Bisphenol analogs on bone health, this research sought to elucidate their impact in conjunction with Melatonin. Using 80 male Sprague Dawley rats, bones were subjected to a 3-point bending test to assess mechanical properties, and histopathological evaluation was conducted after fixation and decalcification. Statistical analysis was performed using SPSS. The results of the mechanical tests revealed significant differences in deformation and elastic modulus values between groups treated with Bisphenol F+Melatonin and Bisphenol S+Melatonin compared to the control groups. However, the histological images showed no significant differences between the groups. In the discussion, it was noted that the injection of Bisphenol F and Melatonin together increased bone hardness, suggesting that Bisphenol F and Bisphenol S may mitigate the negative effects of melatonin on bone. We attributed the absence of histological differences to the male gender of the studied rats and previous exposure considerations. This study shows that Melatonin can reduce Bisphenol F and Bisphenol S' rapid adjustment effects and increase bone elasticity. The side effects of Bisphenol F and S, as well as the prophylactic effects of Melatonin, can be observed and improved by carefully adjusting the duration, dose, and gender selection.

Effect of phased reduction of dietary digestible lysine density on growth performance, thigh meat, and biomechanical characteristics of tibia in broiler chickens.

In this study, growth performance, nutrient intake, thigh meat quality, fatty acid composition of thigh meat, and biomechanical characteristics of tibia of broiler chickens in response to phased restriction of dietary digestible lysine (dLys) were evaluated. A total of 180 male broiler chickens distributed to 3 experimental groups were fed control diets, 85% dLys diet in grower phase (GRO 85% dLys), or 85% dLys diets in grower and finisher phases (GRO-FIN 85% dLys). Feeding 85% dLys suppressed the feed intake that suppressed the growth performance, slaughter weight, and thigh weight of broiler chickens compared to control group (P < 0.05). Average daily dLys, Ca, and P intakes were suppressed in groups fed 85% dLys diets in comparison with control group (P < 0.05) due to the suppression of feed intake. While most fatty acid concentrations in thigh meat were not different among the groups, eicosanoic acid (C20:0) in thigh meat was greater in GRO-FIN 85% dLys group than control group (P = 0.002). Antioxidant status of thigh meat of broiler chickens was not affected by the phased restriction of dietary dLys compared to control group. Bone breaking strength and ultimate strength were greater in control group than 85% dLys groups (P < 0.05). In conclusion, phased dilution of dietary dLys to 85% of the required allowance yields weaker legs and tibia bones by suppressing the Ca and P intakes as a function of reduced feed intake in broiler chickens.

Effect of humate supplementation to feed and/or litter on performance, intestinal viscosity, litter quality, and occurrence of footpad dermatitis in broilers fed barley-based diets.

We investigated the effect of in-feed and/or in-litter supplemental humate against footpad dermatitis (FPD) in broilers fed diets based on barley. Three hundred and sixty 1-day-old Ross 308 broiler chickens were randomly distributed to 24 floor pens (4 treatments, each consisting of 6 replicate pens; 15 chickens per pen) as a completely randomized design with 2 × 2 factorial arrangement of two levels of supplemental humate in feed (0 and 1 g/kg feed) and litter (0 and 5 g/kg litter). Growth performance, intestinal viscosity, litter quality, and incidence and severity of FPD in broilers were measured. In addition, malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined in blood and footpad tissues of broilers with different FPD scores. The results revealed that there was no interaction between humate supplementation to feed and litter. Neither dietary nor litter supplementation of humate had a significant effect on growth performance, intestinal viscosity, litter quality, and occurrence of FPD. And also, MDA and SOD levels in serum and footpad tissue did not affect by either dietary or litter supplementation of humate. The presence of FPD (score 1) had no effect on MDA and SOD levels in serum, however, increased the MDA and SOD levels (P < 0.001, P = 0.001, respectively) in footpad tissue of broilers. The intestinal viscosity did not differ between FPD scores 0 and 1. In conclusion, findings of this experiment suggest that humate supplementation to feed and litter did not alleviate FPD development in broilers fed diets based on barley. In addition, the presence of FPD lesions increases the MDA and SOD levels in the footpad tissues.

Effects of rifampicin on plasma pharmacokinetics of tulathromycin in goats.

We investigated the pharmacokinetic profile of co-administration of tulathromycin with rifampicin. Healthy male goats were allocated to three groups (n = 8) as Group A (single dose 2.5 mg/kg tulathromycin s.c.), B (10 mg kg-1  day-1 rifampicin p.o. daily for 7 days and single dose 2.5 mg/kg tulathromycin s.c. on 8th day), and C (10 mg kg-1  day-1 rifampicin p.o. daily for 21 days and single dose 2.5 mg/kg tulathromycin s.c. on 8th day). Blood samples were collected from jugular veins. Plasma samples were analyzed for tulathromycin by liquid chromatography-mass spectrometry (LC-MS/MS). Peak plasma concentration (Cmax ) values of tulathromycin were 1,390 ± 173, 958 ± 106, and 807 ± 116 ng/ml in groups A, B, and C, respectively. Cmax value of group A was greater than other groups (p < .05). Mean residence time based on time zero to last sample time (MRTlast ) values were 52 ± 1, 56 ± 4 and 66 ± 4 hr in A, B, and C groups, respectively whereas mean residence time based on time zero extrapolated to infinity (MRTINF_obs ) values were 69 ± 4, 85 ± 5, and 86 ± 4 hr, respectively. MRTlast and MRTINF_obs values were greater in B and C groups than group A (p < .05). These findings suggest that rifampicin administration may change several pharmacokinetic parameters of tulathromycin in goats.

Protective Effect of Sodium Selenite on 4-Nonylphenol-Induced Hepatotoxicity and Nephrotoxicity in Rats.

This study was aimed at evaluating the protective effect of sodium selenite (SS) on DNA integrity, antioxidant/oxidant status, and histological changes on 4-nonylphenol (4-NP)-induced toxicity in liver and kidney tissues of rats. Twenty-four adult male Sprague Dawley rats were divided into 4 groups as control, SS, 4-NP, and SS+4-NP group. Control group was untreated. The SS group was supplemented with SS (0.5 mg/kg/day) and the 4-NP group was given 4-NP (125 mg/kg/day). The rats in the SS+4-NP group received SS followed by 4-NP 1 h later at the abovementioned doses. The treatments were administered by oral gavage for 48 days. DNA damage was analyzed by comet assay in lymphocytes. Oxidative stress parameters were measured, and histological evaluation was performed in liver and kidney tissues. Results showed that SS administration significantly decreased % Tail DNA and Mean Tail Moment in SS+4-NP group as compared with 4-NP group. Catalase activity in liver was significantly lower in 4-NP group only. SS treatment significantly increased the glutathione level and decreased high malondialdehyde level in tissues of the SS+4-NP group as compared with 4-NP group. Dilation of central vein, ballooning degeneration, vacuolar degeneration, and deterioration in the structure of remark cords in 4-NP-administered were alleviated in rats that received SS supplementation before administration of 4-NP. Moreover, glycogen intensity in hepatocytes and the wall of central vein increased in the SS+4-NP group. In addition, the SS supplementation in the SS+4-NP group decreased glomerular degeneration as well as the width of cavum glomeruli and congestion intensity in the kidney. These results indicate that SS may have a protective effect against 4-NP-induced hepato-nephrotoxicity in rats.

Dietary quercetin maintains the semen quality in rabbits under summer heat stress.

This study focused to determine beneficial impact of feeding quercetin supplemented diet on semen quality in summer heat imposed rabbits. Twelve heat stressed (HS) adult rabbits bucks were either fed with basal diet (HS; n = 06) or quercetin supplemented diet (QU-HS; n = 06) for a period of 56 days. Semen samples were collected and evaluated for volume, osmolality, morphology, concentration, motility, motion kinetics, viability, acrosome integrity, mitochondrial potential, and seminal plasma MDA level. Semen volume, concentration, motility and sperm kinetics parameters were affected by diet supplementation. Diet affected the sperm mitochondrial potential and day of treatment affected the viable sperm percentage. There was an effect of diet, day of treatment and diet by day interaction on acrosome reaction rate. Sperm head abnormalities were influenced by diet provision, sperm mid-piece abnormalities were affected by diet and day of treatment, whereas, the effect of diet and diet by day of treatment interaction were observed for total sperm abnormalities. There was an effect of diet and diet by day interaction for seminal plasma MDA level. In conclusions, quercetin reduces the damaging effects of HS and maintains the semen quality by lowering the oxidative stress in rabbits.