Tailored extracellular matrix-mimetic coating facilitates reendothelialization and tissue healing of cardiac occluders.

Minimally invasive transcatheter interventional therapy utilizing cardiac occluders represents the primary approach for addressing congenital heart defects and left atrial appendage (LAA) thrombosis. However, incomplete endothelialization and delayed tissue healing after occluder implantation collectively compromise clinical efficacy. In this study, we have customized a recombinant humanized collagen type I (rhCol I) and developed an rhCol I-based extracellular matrix (ECM)-mimetic coating. The innovative coating integrates metal-phenolic networks with anticoagulation and anti-inflammatory functions as a weak cross-linker, combining them with specifically engineered rhCol I that exhibits high cell adhesion activity and elicits a low inflammatory response. The amalgamation, driven by multiple forces, effectively serves to functionalize implantable materials, thereby responding positively to the microenvironment following occluder implantation. Experimental findings substantiate the coating's ability to sustain a prolonged anticoagulant effect, enhance the functionality of endothelial cells and cardiomyocyte, and modulate inflammatory responses by polarizing inflammatory cells into an anti-inflammatory phenotype. Notably, occluder implantation in a canine model confirms that the coating expedites reendothelialization process and promotes tissue healing. Collectively, this tailored ECM-mimetic coating presents a promising surface modification strategy for improving the clinical efficacy of cardiac occluders.

Recent progress in the applications of presynaptic dopaminergic positron emission tomography imaging in parkinsonism.

Nowadays, presynaptic dopaminergic positron emission tomography, which assesses deficiencies in dopamine synthesis, storage, and transport, is widely utilized for early diagnosis and differential diagnosis of parkinsonism. This review provides a comprehensive summary of the latest developments in the application of presynaptic dopaminergic positron emission tomography imaging in disorders that manifest parkinsonism. We conducted a thorough literature search using reputable databases such as PubMed and Web of Science. Selection criteria involved identifying peer-reviewed articles published within the last 5 years, with emphasis on their relevance to clinical applications. The findings from these studies highlight that presynaptic dopaminergic positron emission tomography has demonstrated potential not only in diagnosing and differentiating various Parkinsonian conditions but also in assessing disease severity and predicting prognosis. Moreover, when employed in conjunction with other imaging modalities and advanced analytical methods, presynaptic dopaminergic positron emission tomography has been validated as a reliable in vivo biomarker. This validation extends to screening and exploring potential neuropathological mechanisms associated with dopaminergic depletion. In summary, the insights gained from interpreting these studies are crucial for enhancing the effectiveness of preclinical investigations and clinical trials, ultimately advancing toward the goals of neuroregeneration in parkinsonian disorders.

High-throughput arousing interconnected interfaces for excellent sodium storage chemistry.

Heterostructures endow electrochemical hybrids with promising energy storage properties owing to synergistic effects and interfacial interaction. However, developing a facile but effective approach to maximize interface effects is crucial but challenging. Herein, a bimetallic sulfide/carbon heterostructure is realized in a confined carbon network via a high-throughput template-assisted strategy to induce highly active and stable electrode architecture. The designed heterostructures not only yield abundant interconnected Co9S8/MoS2/N-doped carbon (Co9S8/MoS2/NC) heterojunctions with continuous channels for ion/electron transfer but maintain excellent conversion reversibility. Serving as anode for sodium storage, the Co9S8/MoS2/NC framework displayed excellent sodium storage properties (reversible capacity of 480 mAh/g after 100 cycles at 0.2 A/g and 286.2 mAh/g after 500 cycles at 2 A/g). Given this, this study can guide future design protocols for interface engineering by forming dynamic channels of conversion reaction kinetics for potential applications in high-performance electrodes.

Association between genetic polymorphisms in chromosome region 9q21 and pelvic organ prolapse in Northwestern Chinese women.

This study aimed to explore the association between genetic polymorphisms in the chromosome region 9q21 and the risk of pelvic organ prolapse (POP) in Northwestern Chinese women. A case-control study was conducted with 241 POP patients and 268 healthy controls, analyzing ten single nucleotide polymorphisms (SNPs) across five genes using PCR amplification and Sequenom MassArray. The results revealed significant associations between three SNPs-rs2297002 in GOLM1, rs7450 in MAK10, and rs3814535 in TLE1-and POP. Specifically, the TC genotype of rs2297002, the GA genotype of rs7450, and the AA genotype of rs3814535 were linked to an increased or decreased risk of POP. The study suggests that these genetic variants might contribute to the pathogenesis of POP in this population, offering potential markers for early diagnosis and further investigation into the molecular mechanisms underlying POP.

Cette étude visait à explorer l'association entre les polymorphismes génétiques dans la région chromosomique 9q21 et le risque de prolapsus des organes pelviens (POP) chez les femmes chinoises du nord-ouest. Une étude cas-témoins a été menée auprès de 241 patientes atteintes de POP et de 268 témoins sains, analysant dix polymorphismes nucléotidiques simples (SNP) sur cinq gènes à l'aide de l'amplification par PCR et du Sequenom MassArray. Les résultats ont révélé des associations significatives entre trois SNP (rs2297002 dans GOLM1, rs7450 dans MAK10 et rs3814535 dans TLE1) et le POP. Plus précisément, le génotype TC de rs2297002, le génotype GA de rs7450 et le génotype AA de rs3814535 étaient liés à un risque accru ou réduit de POP. L'étude suggère que ces variantes génétiques pourraient contribuer à la pathogenèse du POP dans cette population, offrant des marqueurs potentiels pour un diagnostic précoce et une étude plus approfondie des mécanismes moléculaires sous-jacents au POP.

Chemical Synthesis, Herbicidal Activity, Crop Safety, and Molecular Basis of ortho-Fluoroalkoxy Substituted Sulfonylureas as Novel Acetohydroxyacid Synthase Inhibitors.

In the face of increasing resistance to the currently used commercial herbicides and the lack of success in identifying new herbicide targets, alternative herbicides need to be developed to control unwanted monocotyledon grasses in food crops. Here, a panel of 29 novel sulfonylurea-based compounds with ortho-fluoroalkoxy substitutions at the phenyl ring were designed and synthesized. Pot assays demonstrated that two of these compounds, 6d and 6u, have strong herbicidal activities against Echinochloa crus-galli, Eleusine indica, Alopecurus aequalis, and Alopecurus japonicus Steudel at a dosage of 15 g ha-1. Furthermore, these two compounds exhibited <5% inhibition against wheat at a dosage of 30 g ha-1 under post-emergence conditions. 6u also exhibited <5% inhibition against rice at a dosage of 30 g ha-1 under both post-emergence and pre-emergence conditions. A kinetics study demonstrated that 6d and 6u are potent inhibitors of Arabidopsis thaliana acetohydroxyacid synthase (AHAS; EC 2.2.1.6) with potent Ki values of 18 ± 1.1 and 11.9 ± 4.0 nM, respectively. The crystal structure of 6u in complex with A. thaliana (At)AHAS has also been determined at 2.7 Å resolution. These new compounds represent new alternative herbicide choices to protect wheat or rice from invading grasses.

Management of Severe Dysphonia and Dysphagia Following Lateral Skull Base Surgery.

OBJECTIVES: Lateral skull base surgeries pose a risk of injuring the lower cranial nerves, leading to potential postoperative complications such as dysphonia and dysphagia. Conservative treatments have shown limited efficacy in addressing these resultant voice and swallowing dysfunctions, significantly impacting patient quality of life. This study aims to evaluate the safety and effectiveness of a combined surgical approach involving autologous fat injection laryngoplasty (AFIL) and transcervical cricopharyngeal myotomy (TCPM) in patients suffering from severe dysphonia and dysphagia following lateral skull base surgery. METHODS: A retrospective analysis was conducted on 16 patients who underwent concurrent AFIL and TCPM to improve severe dysphonia and dysphagia following lateral skull base surgery. Preoperative and postoperative assessments of voice and swallowing functions were performed using the Voice Handicap Index-10 (VHI-10), GRBAS scale, maximum phonation time (MPT), the Chinese version of Swallow Quality-of-Life Questionnaire (CSWAL-QOL), and videofluoroscopic swallowing studies (VFSS). RESULTS: The results demonstrated notable improvements in voice quality and swallowing function. The VHI-10 score improved significantly from a preoperative mean of 32.06 ± 4.92 to a postoperative 9.06 ± 5.24. The results of the perceptual parameters of the GRBAS scale also improved significantly. The MPT increased from a preoperative average of 3.91 ± 1.00 seconds to 9.14 ± 2.44 seconds postoperatively. The CSWAL-QOL scores significantly improved from a preoperative score of 92.44 ± 17.75 to 130.19 ± 26.07 postoperatively. The VFSS-SWAL scores decreased from 6.63 ± 1.36 before surgery to 3.56 ± 1.58 after surgery. Similarly, the Penetration Aspiration Scale (PAS) scores significantly dropped from 6.38 ± 1.05 preoperatively to 2.93 ± 1.48 postoperatively. Nine out of 11 patients were able to have their gastric tubes successfully removed after surgery. There were no significant postoperative complications. CONCLUSION: Concurrent AFIL and TCPM present a promising reconstructive method for patients experiencing severe dysphonia and dysphagia following lateral skull base surgery, highlighting its value in the postoperative management of complex lower cranial nerve injuries.

Construction of knowledge sharing network indicator system for medication therapy management service training teams based on social network analysis.

BACKGROUND: Based on the perspective of social network theory, this study explored the network indicator system that facilitated optimal knowledge sharing effect in Medication Therapy Management Services (MTMS) training teams. The aim was to provide a reference for optimizing MTMS training and improving training quality. METHODS: Utilizing social network analysis combined with a questionnaire survey, a knowledge sharing matrix for MTMS training teams was constructed. Knowledge sharing behavior was assessed from three perspectives: individual networks, whole networks, and cohesive subgroups. RESULTS: Individual network analysis showed that the knowledge sharing effect within the training team reached its peak when the out-degree centrality was ≥ 3.5, in-degree centrality was ≥ 2.5, eigenvector centrality was ≥ 0.065, and closeness centrality was ≥ 7.86. Whole network analysis indicated that the optimal knowledge sharing effect occurred when the network density of the training team was higher than 0.0343 and the training size was less than 117 participants. Cohesion subgroups analysis demonstrated that knowledge sharing was more effective when members with similar working years participated in training together. CONCLUSIONS: The knowledge sharing indicator system developed for MTMS training teams, based on social network analysis, can assist in optimizing the MTMS training model and improving training effectiveness.

Multi-level exploration of auricular acupuncture: from traditional Chinese medicine theory to modern medical application.

As medical research advances and technology rapidly develops, auricular acupuncture has emerged as a point of growing interest. This paper delves into the intricate anatomy of auricular points, their significance and therapeutic principles in traditional Chinese medicine (TCM), and the underlying mechanisms of auricular acupuncture in contemporary medicine. The aim is to delve deeply into this ancient and mysterious medical tradition, unveiling its multi-layered mysteries in the field of neurostimulation. The anatomical structure of auricular points is complex and delicate, and their unique neurovascular network grants them a special status in neurostimulation therapy. Through exploration of these anatomical features, we not only comprehend the position of auricular points in TCM theory but also provide a profound foundation for their modern medical applications. Through systematic review, we synthesize insights from traditional Chinese medical theory for modern medical research. Building upon anatomical and classical theoretical foundations, we focus on the mechanisms of auricular acupuncture as a unique neurostimulation therapy. This field encompasses neuroregulation, pain management, psychological wellbeing, metabolic disorders, and immune modulation. The latest clinical research not only confirms the efficacy of auricular stimulation in alleviating pain symptoms and modulating metabolic diseases at the endocrine level but also underscores its potential role in regulating patients' psychological wellbeing. This article aims to promote a comprehensive understanding of auricular acupuncture by demonstrating its diverse applications and providing substantial evidence to support its broader adoption in clinical practice.

FGF21 inhibits ferroptosis caused by mitochondrial damage to promote the repair of peripheral nerve injury.

Introduction: Ferroptosis is a new type of cell death characterized by lipid peroxidation and iron dependency, representing an emerging disease regulation mechanism. The limited understanding of ferroptosis in peripheral nerve injury (PNI) complicates the management of such injuries. Mitochondrial dysfunction, which contributes to ferroptosis, further exacerbates the challenges of peripheral nerve repair. Methods: In this study, we established an in vitro model of Schwann cells model treated with TBHP and an in vivo sciatic nerve crush injury model in rats. These models were used to investigate the effects of fibroblast growth factor 21 (FGF21) on PNI, both in vitro and in vivo, and to explore the potential mechanisms linking injury-induced ferroptosis and mitochondrial dysfunction. Results: Our findings reveal that PNI triggers abnormal accumulation of lipid reactive oxygen species (ROS) and inactivates mitochondrial respiratory chain complex III, leading to mitochondrial dysfunction. This dysfunction catalyzes the oxidation of excessive polyunsaturated fatty acids, resulting in antioxidant imbalance and loss of ferroptosis suppressor protein 1 (FSP1), which drives lipid peroxidation. Additionally, irregular iron metabolism, defective mitophagy, and other factors contribute to the induction of ferroptosis. Importantly, we found that FGF21 attenuates the abnormal accumulation of lipid ROS, restores mitochondrial function, and suppresses ferroptosis, thus promoting PNI repair. Notably, glutathione peroxidase 4 (GPX4), a downstream target of nuclear factor E2-related factor 2 (Nrf2), and the ERK/Nrf2 pathway are involved in the regulation of ferroptosis by FGF21. Conclusion: FGF21 promotes peripheral nerve repair by inhibiting ferroptosis caused by mitochondrial dysfunction. Therefore, targeting mitochondria and ferroptosis represents a promising therapeutic strategy for effective PNI repair.

Experimental realization of multiple frequency photoassociation in an optical dipole trap.

The generation of cold molecules is an important topic in the field of cold atoms and molecules and has received relevant advanced research attention in ultracold chemistry, quantum computation, and quantum metrology. With a high atomic phase space density, optical dipole traps have been widely used to prepare, trap, and study cold molecules. In this work, Rb2 molecules were photoassociated in a magneto-optical trap to obtain a precise rovibrational spectrum, which provided accurate numerical references for the realization of multiple frequency photoassociation. By meeting the harsh requirements of photoassociation in optical dipole traps, the cold molecule photoassociation process was well explored, and different photoassociation resonances were simultaneously addressed in a single optical dipole trap. This method can be universally extended to simultaneously photoassociate cold molecules with different internal states or atomic species in a single optical dipole trap, thus advancing generous cold molecule studies such as cold molecule collision dynamics.

A bilayer hydrogel mimicking the periosteum-bone structure for innervated bone regeneration.

In bone tissue, nerves are primarily located in the periosteum and play an indispensable role in bone defect repair. However, most bone tissue engineering approaches ignored the reconstruction of the nerve network. Herein, we aimed to develop a bilayer hydrogel simulating periosteum-bone structure to induce innervated bone regeneration. The bottom "bone" layer consisted of gelatin methacryloyl (GelMA), poly(ethylene glycol) diacrylate (PEGDA), and nano-hydroxyapatite (nHA), whereas the upper "periosteum" layer consisted of GelMA, sodium alginate (SA) and MgCl2. The mechanical properties of the upper and bottom hydrogels were designed to be suitable for neurogenesis and osteogenesis, respectively. Besides, Mg2+ from the "periosteum" layer released at the early stage (within 7 d), which aligned with the optimal time window for nerve regeneration and osteogenic related neuropeptide release. Simultaneously, the prevention of long-term Mg2+ release (after 7 d) could avoid osteogenic inhibition caused by prolonged Mg2+ exposure. Additionally, the incorporation of nHA in the bottom "bone" layer supported the long-term osteogenesis due to its osteoconductivity and slow degradation. In vitro biological experiments revealed that the bilayer hydrogel (GS@Mg/GP@nHA) promoted neurite growth and calcitonin gene-related peptide (CGRP) expression in rat dorsal root ganglion (DRG) neurons, as well as the osteogenesis of rat bone-derived mesenchymal stem cells (BMSCs). Moreover, the in vivo experiments demonstrated that the GS@Mg/GP@nHA hydrogel efficiently promoted nerve network reconstruction and bone regeneration of rat calvarial bone defects. Altogether, the bilayer hydrogel GS@Mg/GP@nHA could promote innervated bone regeneration, providing new insights for biomaterial design for bone tissue engineering.

The Association Between Changes in Body Mass Index and the Risk of Depression.

Objective: In recent years, the risk of depression has increased among young people, and changes in body mass index (BMI) during childhood may be important factors in their development. However, the relationship between changes in BMI during childhood and the risk of depression needs further research and exploration. Methods: The annual health examination data were collected from Physical Examination Center of Wuhan Mental Health Center Hospital, including 1226 students. The height and weight of students at the age of 11, 14, and 17 were recorded in sequence, and at 18 years old, these students were followed up according to the Hamilton Depression Scale (HAMD) to evaluate the depression. The relationship between BMI trends and depression was analyzed through Logistic regression analysis. Results: The growth trend of BMI was divided into normal growth, slow growth, and excessive growth. The odds ratio (OR) value for depression in the slow growth was 1.218 (95% CI, 0.995-1.493) compared to the normal growth, which was no significant difference (P = .056). The OR value for depression in the excessive growth was 1.982 (95% CI, 1.243-3.177) compared to the normal growth, which was significant difference (P = .003). Conclusion: The rapid growth of BMI is correlated with the occurrence of depression in young individuals and may be a contributing factor to the development of depression in this demographic.

The T-Type Calcium Channel CACNA1H is Required for Smooth Muscle Cytoskeletal Organization During Tracheal Tubulogenesis.

Abnormalities of tracheal smooth muscle (SM) formation are associated with several clinical disorders including tracheal stenosis and tracheomalacia. However, the cellular and molecular mechanisms underlying tracheal SM formation remain poorly understood. Here, it is shown that the T-type calcium channel CACNA1H is a novel regulator of tracheal SM formation and contraction. Cacna1h in an ethylnitrosourea forward genetic screen for regulators of respiratory disease using the mouse as a model is identified. Cacna1h mutants exhibit tracheal stenosis, disorganized SM and compromised tracheal contraction. CACNA1H is essential to maintain actin polymerization, which is required for tracheal SM organization and tube formation. This process appears to be partially mediated through activation of the actin regulator RhoA, as pharmacological increase of RhoA activity ameliorates the Cacna1h-mutant trachea phenotypes. Analysis of human tracheal tissues indicates that a decrease in CACNA1H protein levels is associated with congenital tracheostenosis. These results provide insight into the role for the T-type calcium channel in cytoskeletal organization and SM formation during tracheal tube formation and suggest novel targets for congenital tracheostenosis intervention.

Learning to Explain is a Good Biomedical Few-Shot Learner.

MOTIVATION: Significant progress has been achieved in biomedical text mining using deep learning methods, which rely heavily on large amounts of high-quality data annotated by human experts. However, the reality is that obtaining high-quality annotated data is extremely challenging due to data scarcity (e.g., rare or new diseases), data privacy and security concerns, and the high cost of data annotation. Additionally, nearly all researches focus on predicting labels without providing corresponding explanations. Therefore, in this paper, we investigate a more realistic scenario, biomedical few-shot learning, and explore the impact of interpretability on biomedical few-shot learning. RESULTS: We present LetEx-Learning to explain-a novel multi-task generative approach that leverages reasoning explanations from large language models (LLMs) to enhance the inductive reasoning ability of few-shot learning. Our approach includes (1) collecting high-quality explanations by devising a suite of complete workflow based on LLMs through CoT prompting and self-training strategies. (2) converting various biomedical NLP tasks into a text-to-text generation task in a unified manner, where collected explanations serve as additional supervision between text-label pairs by multi-task training. Experiments are conducted on 3 few-shot settings across 6 biomedical benchmark datasets. The results show that learning to explain improves the performances of diverse biomedical NLP tasks in low-resource scenario, outperforming strong baseline models significantly by up to 6.41%. Notably, the proposed method makes the 220M LetEx perform superior reasoning explanation ability against LLMs. AVAILABILITY: Our source code and data are available at https://github.com/cpmss521/LetEx. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Comparative analysis of safety and effectiveness between natural orifice specimen extraction and conventional transabdominal specimen extraction in robot-assisted colorectal cancer resection through systematic review and meta-analysis.

The goal of this systematic review and meta-analysis is to evaluate the perioperative and oncologic results of natural orifice specimen extraction (NOSE) compared to conventional transabdominal specimen extraction (TASE) in robotic-assisted surgery for colorectal cancer. A comprehensive electronic search will be performed on PubMed, Embase, and the Cochrane Library to find research articles published from the beginning of the databases to July 2024 that focus on patients who have undergone robotic-assisted surgery for colorectal cancer. Specifically, this review will compare NOSE with conventional TASE. Only studies published in English will be considered. Literature screening will adhere closely to predetermined criteria for inclusion and exclusion, specifically targeting randomized controlled trials and cohort studies. The evaluation of quality will involve the use of the Newcastle-Ottawa Scale (NOS). Meta-analysis of the included studies' data will be performed using Review Manager 5.4.1. In the final analysis, 9 retrospective cohort studies comprising 1571 patients were included. Out of these, 732 patients opted for NOSE, while 839 patients chose conventional TASE in robotic colorectal surgery. Patients who received TASE experienced enhancements in hospital stay duration, time until first gas passage, wound infection rates, and time until the first intake of a liquid diet. Nevertheless, there were no notable distinctions noted between the two methods regarding surgery duration, projected blood loss, intestinal blockage, or frequency of anastomotic leakage. In patients undergoing robotic-assisted colorectal surgery, the safety and feasibility of NOSE are demonstrated. Compared to traditional TASE, it provides clear benefits including shorter hospital stays, earlier first flatus, quicker initiation of a liquid diet, and lower risk of wound infection.

A novel binary data classification algorithm based on the modified reaction-diffusion predator-prey system with Holling-II function.

In this study, we propose a modified reaction-diffusion prey-predator model with a Holling-II function for binary data classification. In the model, we use u and v to represent the densities of prey and predators, respectively. We modify the original equation by substituting the term v with f-v to obtain a stable and clear nonlinear decision surface. By employing a finite difference method for numerical solution of the original model, we conduct various experiments in two-dimensional and three-dimensional spaces to validate the feasibility of the classifier. Additionally, with consideration for wide real applications, we perform classification experiments on electroencephalogram signals, demonstrating the effectiveness and robustness of the classifier in binary data classification.

Spoofing attack recognition for GNSS-based train positioning using a BO-LightGBM method.

Trustworthy positioning is critical in the operational control and management of trains. For a train positioning system (TPS) based on a global navigation satellite system (GNSS), a spoofing attack significantly threatens the trustworthiness of positioning. However, the influence and recognition of GNSS spoofing attacks are not considered in the existing research on GNSS-enabled TPS. Spoofing attacks affect the performance of GNSS observations and the positioning results, allowing the development of data-driven spoofing recognition solutions. This study aims to achieve effective spoofing recognition for active security protection in TPS. Different features were designed to reflect the effects of a spoofing attack, including GNSS observation-related indicators and odometer-enabled parameters, and a novel Bayesian optimization-light gradient boosting machine (BO-LightGBM) solution was proposed. In particular, a Bayesian optimization technique was introduced into the LightGBM framework to improve the hyperparameter determination capability for recognition model training. Using a GNSS spoofing test platform with a specific GNSS signal generator and the SimSAFE spoofing test tool, different spoofing attack modes were tested to collect sample datasets for model training and evaluation. The results of model establishment and comparison of the model performance indicators illustrated the advantages of the proposed solution, its adaptability to different spoofing attack situations, and its superiority over state-of-the-art modeling strategies.

Unraveling chemical origins of dendrite formation in zinc-ion batteries via in situ/operando X-ray spectroscopy and imaging.

To prevent zinc (Zn) dendrite formation and improve electrochemical stability, it is essential to understand Zn dendrite growth, particularly in terms of morphology and relation with the solid electrolyte interface (SEI) film. In this study, we employ in-situ scanning transmission X-ray microscopy (STXM) and spectro-ptychography to monitor the morphology evolution of Zn dendrites and to identify their chemical composition and distribution on the Zn surface during the stripping/plating progress. Our findings reveal that in 50 mM ZnSO4, the initiation of moss/whisker dendrites is chemically controlled, while their continued growth over extended cycles is kinetically governed. The presence of a dense and stable SEI film is critical for inhibiting the formation and growth of Zn dendrites. By adding 50 mM lithium chloride (LiCl) as an electrolyte additive, we successfully construct a dense and stable SEI film composed of Li2S2O7 and Li2CO3, which significantly improves cycling performance. Moreover, the symmetric cell achieves a prolonged cycle life of up to 3900 h with the incorporation of 5% 12-crown-4 additives. This work offers a strategy for in-situ observation and analysis of Zn dendrite formation mechanisms and provides an effective approach for designing high-performance Zn-ion batteries.

Cystathionine-γ-lyase contributes to tamoxifen resistance, and the compound I194496 alleviates this effect by inhibiting the PPARγ/ACSL1/STAT3 signalling pathway in oestrogen receptor-positive breast cancer.

Tamoxifen (TAM) resistance is a major challenge in treating oestrogen receptor-positive (ER+) breast cancers. It is possible that the H2S synthase cystathionine-γ-lyase (CSE), which has been previously shown to promote tumour growth and metastasis in other cancer cells, is involved in this resistance. Therefore, we investigated CSE's role and potential mechanisms in TAM-resistant breast cancer cells. First, we examined the effect of CSE expression on TAM sensitivity and resistance in MCF7 (breast cancer) cells. The findings revealed that CSE was directly associated with TAM sensitivity and involved in TAM resistance in ER+ breast cancer cells, indicating that it may be useful as a biomarker. Next, we wanted to determine the molecular mechanism of CSE's role in TAM resistance. Using cell migration, co-immunoprecipitation, western blotting, and cell viability assays, we determined that the CSE/H2S system can affect the expression of PPARγ by promoting the sulfhydrylation of PPARγ, which regulates the transcriptional activity of ACSL1. ACSL1, in turn, influences STAT3 activation by affecting the phosphorylation, palmitoylation and dimerization of STAT3, ultimately leading to the development of TAM resistance in breast cancer. Finally, we examined the effect of CSE inhibitors on reducing drug resistance to determine whether CSE may be used as a biomarker of TAM resistance. We observed that the novel CSE inhibitor I194496 can reverse TAM resistance in TAM-resistant breast cancer via targeting the PPARγ/ACSL1/STAT3 signalling pathway. Overall, our data indicate that CSE may serve as a biomarker of TAM resistance and that the CSE inhibitor I194496 is a promising candidate for combating TAM resistance.

Fabrication of bamboo nanocellulose fibril-based food packaging with dual-antimicrobial property.

The development of cellulose-based packaging films with excellent antimicrobial properties and biocompatibility has garnered significant attention. In this work, nanocellulose fibrils (NCFs) derived from from bamboo parenchyma cells were utilized to fabricate nanocomposite film with antimicrobial properties. This system exhibited distinct release behaviors for two antimicrobial agents, with the slow release of Ag nanoparticle (AgNP) in the initial stage contributed to delaying food spoilage, while the subsequent pH change in the microenvironment facilitated the release of essential oil of sour orange blossoms (SEO) for secondary antimicrobial activity. Additionally, the composite film demonstrated improved thermal stability and UV blocking capacity. Moreover, AgNP has been proven to enhance the mechanical properties, with the tensile strength of the novel composite film increasing by 34.85 % compared to control group. The water vapor permeability and oxygen permeability of the novel composite film were reduced, which could potentially reduce weight loss and slow down the rate of after-ripening. Following the acidification treatment, the films containing EO@MPN (essential oil encapsulated with metal-polyphenol network) component performed different antimicrobial patterns, indicating their pH-responsive antimicrobial capabilities, and they are effective against both Gram-positive and Gram-negative bacteria. After a 24-h exposure to a food simulant, the release amount of Ag was measured at 67.6 µg/dm2, within the acceptable limit, and the release profile of Ag was characterized. Cytotoxicity and Live/Dead staining tests confirmed that the novel composite film film had no significant toxicity, thus making it safe for application in food preservation. Furthermore, in a 15-day preservation experiment with mangoes, the novel composite film demonstrated the best performance, underscoring its potential as a sustainable antimicrobial packaging material.