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1.
Acta Cir Bras ; 31(7): 448-55, 2016 Jul.
Article in English | MEDLINE | ID: mdl-27487279

ABSTRACT

PURPOSE: To determine whether Toll-like receptor 7 (TLR7) is the potential targets of prevention or progression in the renal ischemia/reperfusion (I/R) injury of STZ-induced diabetic rats. METHODS: Thirty six Sprague-Dawley rats were randomly arranged to the nondiabetic (ND) or diabetic group (DM), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and CD (given by Chloroquine) group. Preoperatively, Chloroquine (40 mg/kg, intraperitoneal injection.) was administrated 6 days for treatment group. I/R animals were subjected to 25 min of bilateral renal ischemia. Renal function, histology, apoptosis, cytokines, expression of TLR7, MyD88 and NF-κB were detected. RESULTS: The serum levels of blood urea nitrogen, creatinine, IL-6 and TNF-α, apoptotic tubular epithelial cells, expression of TLR7, MyD88 and NF-κB were significantly increased in DM+I/R group, compared with ND+I/R group (p<0.05). All these changes were further improved by TLR7 inhibition Chloroquine except Paller scores (p<0.05). CONCLUSION: Toll-like receptor 7 inhibition attenuates the acute renal ischemia/reperfusion injury of STZ-induced diabetic in SD rats.


Subject(s)
Acute Kidney Injury/metabolism , Diabetes Mellitus, Experimental/metabolism , Kidney/metabolism , Reperfusion Injury/metabolism , Toll-Like Receptor 7/metabolism , Acute Kidney Injury/pathology , Animals , Apoptosis , Diabetes Mellitus, Experimental/complications , Disease Models, Animal , In Situ Nick-End Labeling/methods , Kidney/pathology , Male , Myeloid Differentiation Factor 88/metabolism , NF-kappa B/metabolism , Random Allocation , Rats, Sprague-Dawley , Reperfusion Injury/complications , Toll-Like Receptor 7/blood
2.
Acta cir. bras ; Acta cir. bras;31(7): 448-455, graf
Article in English | LILACS | ID: lil-787258

ABSTRACT

ABSTRACT PURPOSE: To determine whether Toll-like receptor 7 (TLR7) is the potential targets of prevention or progression in the renal ischemia/reperfusion (I/R) injury of STZ-induced diabetic rats. METHODS: Thirty six Sprague-Dawley rats were randomly arranged to the nondiabetic (ND) or diabetic group (DM), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and CD (given by Chloroquine) group. Preoperatively, Chloroquine (40 mg/kg, intraperitoneal injection.) was administrated 6 days for treatment group. I/R animals were subjected to 25 min of bilateral renal ischemia. Renal function, histology, apoptosis, cytokines, expression of TLR7, MyD88 and NF-κB were detected. RESULTS: The serum levels of blood urea nitrogen, creatinine, IL-6 and TNF-α, apoptotic tubular epithelial cells, expression of TLR7, MyD88 and NF-κB were significantly increased in DM+I/R group, compared with ND+I/R group (p<0.05). All these changes were further improved by TLR7 inhibition Chloroquine except Paller scores (p<0.05). CONCLUSION: Toll-like receptor 7 inhibition attenuates the acute renal ischemia/reperfusion injury of STZ-induced diabetic in SD rats.


Subject(s)
Animals , Male , Reperfusion Injury/metabolism , Diabetes Mellitus, Experimental/metabolism , Toll-Like Receptor 7/metabolism , Acute Kidney Injury/metabolism , Kidney/metabolism , Reperfusion Injury/complications , Random Allocation , NF-kappa B/metabolism , Rats, Sprague-Dawley , Apoptosis , In Situ Nick-End Labeling/methods , Diabetes Mellitus, Experimental/complications , Disease Models, Animal , Toll-Like Receptor 7/blood , Myeloid Differentiation Factor 88/metabolism , Acute Kidney Injury/pathology , Kidney/pathology
3.
Acta cir. bras. ; 31(7): 448-455, July 2016. ilus, graf
Article in English | VETINDEX | ID: vti-21026

ABSTRACT

PURPOSE:To determine whether Toll-like receptor 7 (TLR7) is the potential targets of prevention or progression in the renal ischemia/reperfusion (I/R) injury of STZ-induced diabetic rats.METHODS:Thirty six Sprague-Dawley rats were randomly arranged to the nondiabetic (ND) or diabetic group (DM), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and CD (given by Chloroquine) group. Preoperatively, Chloroquine (40 mg/kg, intraperitoneal injection.) was administrated 6 days for treatment group. I/R animals were subjected to 25 min of bilateral renal ischemia. Renal function, histology, apoptosis, cytokines, expression of TLR7, MyD88 and NF-κB were detected.RESULTS:The serum levels of blood urea nitrogen, creatinine, IL-6 and TNF-α, apoptotic tubular epithelial cells, expression of TLR7, MyD88 and NF-κB were significantly increased in DM+I/R group, compared with ND+I/R group (p<0.05). All these changes were further improved by TLR7 inhibition Chloroquine except Paller scores (p<0.05).CONCLUSION:Toll-like receptor 7 inhibition attenuates the acute renal ischemia/reperfusion injury of STZ-induced diabetic in SD rats.(AU)


Subject(s)
Animals , Rats , Toll-Like Receptor 7 , Reperfusion Injury/veterinary , Acute Kidney Injury/veterinary , Mice, Inbred NOD , Diabetes Mellitus, Experimental , Rats, Sprague-Dawley
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