Optimization of Hypericum Perforatum Microencapsulation Process by Spray Drying Method.

Hypericum perforatum (HP) contains valuable and beneficial bioactive compounds that have been used to treat or prevent several illnesses. Encapsulation technology offers protection of the active compounds and facilitates to expose of the biologically active compounds in a controlled mechanism. Microcapsulation of the hydroalcoholic gum arabic and maltodextrin have hot been used as wall materials in the encapsulation of HP extract. Therefore, the optimum microencapsulation parameters of Hypericum perforatum (HP) hydroalcoholic extract were determined using response surface methodology (RSM) for the evaluation of HP extract. Three levels of three independent variables were screened using the one-way ANOVA. Five responses were monitored, including total phenolic content (TPC), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), carr index (CI), hausner ratio (HR), and solubility. Optimum drying conditions for Hypericum perforatum microcapsules (HPMs) were determined: 180 °C for inlet air temperature, 1.04/1 for ratio of maltodextrin to gum arabic (w/w), and 1.98/1 for coating to core material ratio (w/w). TPC, antioxidant activity, CI, HR, and solubility values were specified as 316.531 (mg/g GAE), 81.912%, 6.074, 1.066, and 35.017%, respectively, under the optimized conditions. The major compounds of Hypericum perforatum (hypericin and pseudohypericin) extract were determined as 4.19 µg/g microcapsule and 15.09 µg/g microcapsule, respectively. Scanning electron microscope (SEM) analysis revealed that the mean particle diameter of the HPMs was 20.36 µm. Based on these results, microencapsulation of HPMs by spray drying is a viable technique which protects the bioactive compounds of HP leaves, facilitating its application in the pharmaceutical, cosmetic, and food industries.

Three-dimensional control of alluvial fans by rock uplift in an extensional regime: Aydin Range, Aegean extensional province.

Tectonics imparts a first-order control on the overall morphology of alluvial fan systems in extensional settings by influencing sediment flux and accommodation space, while other factors such as climate, catchment lithology, and fault footwall characteristics are secondary. Previous alluvial fan modeling studies have focused on the link between the three-dimensional development of alluvial fans and rock uplift, however, despite the potential influence of tectonics on the overall three-dimensional morphology of alluvial fans, the controlling mechanisms, as well as their relative importance, remain largely unquantified in a natural setting with a targeted source-to-sink approach. Here, we examine 45 alluvial fans and their catchments along the southern mountain front of the Aydin Range, delimited by segmented normal faults in the western Anatolia Extensional Province, to quantify the role of rock uplift. We quantify river incision rates and catchment-wide erosion rates together with a series of topographic analyses across the southern flank of the Aydin Range as a proxy for rock uplift. Our results indicate that the spatial distribution of thicker and steeper alluvial fans fit well with higher rock uplift rates along the strike of the mountain front. In contrast, a lower uplift rate is responsible for prograding alluvial fans with decreasing thickness and gradients. Also, our data shows that alluvial fan thickness compared to other alluvial fan metrics strongly associated with the pattern of the rock uplift. This study demonstrates a field-based, quantitative linkage between three-dimensional alluvial fan morphology and rock uplift which has significant implications for improving alluvial fan models and understanding how alluvial fans respond to tectonics in extensional regions.

Dilemma of Supra- or Infrapatellar Tibial Nailing: Anterior Knee Pain vs. Intra-Articular Damage.

Aim: Intramedullary nailing (IMN) is widely accepted as the treatment of choice for tibial fractures, and a suprapatellar method has been described to prevent common problems associated with the typical infrapatellar IMN technique, such as anterior knee pain. However, in the suprapatellar technique, injury to intra-articular structures is a concern. The aim of this study was to compare the clinical and radiological results of suprapatellar and infrapatellar IMN in terms of union, complications, and function. Methods: A retrospective evaluation of 61 patients who had undergone suprapatellar (n = 29, Group A) or infrapatellar (n = 31, Group B) tibial IMN was conducted. For the suprapatellar group, magnetic resonance imaging scans were acquired on the sixth month follow-up. Complications, radiological findings, functional outcomes, surgery duration, and differences in a range of motion (ROM) were compared. Results: Surgery duration was significantly shorter in Group A (81 mins vs. 107 mins, p < 0.001), and visual analog scale (VAS) values were significantly higher in Group B (0.17 vs. 1.62, p < 0.001). In Group A, the patients' Lysholm scores were significantly higher (95.6 vs. 92, p=0.006). In terms of anterior knee pain, none was experienced in Group A (0%), while 11 patients (26.1%) reported about it in Group B. There were no statistically significant differences between the two groups in SF-36 score (p=0.925), the radiographic union scale in tibial (RUST) fractures score (p=0.454), union time (p=0.110), or ROM (p=0.691). In Group A, two cases of patellofemoral cartilage degeneration were observed. Conclusion: If performed with sufficient expertise, the suprapatellar IMN technique is a safe, reliable technique with a low frequency of anterior knee pain for treating tibial fractures. There is no clear evidence that it causes damage to intra-articular structures. The possibility of patellofemoral cartilage degeneration due to this technique should be further evaluated by prospective studies including pre- and postoperative radiologic assessments.

Comparison of USG-guided or landmark approach fascia iliaca compartment block for positioning in elderly hip fracture patients with spinal anesthesia: a randomized controlled observational study

Background/aim: Currently, the elderly population in the world is rapidly increasing due to technological developments and convenient access to health services. Due to comorbidities in elderly patients, hip fractures are frequently observed after exposure to environmental trauma. To reduce pain during positioning in spinal anesthesia, fascia iliaca compartment block (FICB) can be applied easily and reliably. In our study, we aimed to compare the analgesic effects and duration of fascia iliaca compartment blocks performed with USG guidance or the landmark approach methods for relieving spinal anesthesia position pain. Materials and methods: Our study included 100 patients undergoing operations due to hip fracture and administered spinal anesthesia after FICB. The group with USG-guided FICB (USG) had the blockage needle advanced to the compartment under the fascia iliaca, and 15 mL bupivacaine + 10 mL 2% lidocaine was administered. They were placed in sitting position for spinal anesthesia 20 min later and procedure duration and numerical rating scale (NRS) scores were recorded. In the group with landmark approach FICB (LAND), the spina iliaca anterior superior (SIAS) and pubic tubercle were connected with a line. The same amount of local anesthetic was administered to the external 1/3 portion of this line with the double pop technique. Procedure duration and NRS scores were recorded. Results: There was no statistically significant difference between the two groups in terms of NRS scores (p: 0.073). There was a statistically significant difference in duration of FICB administration between the two groups (p < 0.001). Conclusion: Both USG-guided and landmark approach FICB methods provide adequate and similar analgesia for positioning in spinal anesthesia. However, in cases where there is no problem with access to the ultrasound device or time, safer blockage can be provided by imaging neurovascular structures with ultrasound.

A different option in vascular bone grafts in the hand and wrist region: Use of a dorsoulnar artery-based osteo-fascio-cutaneous flap: A case series.

OBJECTIVES: This study aims to describe our surgery technique and discuss patients treated through the dorsoulnar artery (DUA)-based technique of osseous and osteo-fascio-cutaneous vascularized ulnar bone grafting. PATIENTS AND METHODS: Between January 2011 and January 2015, six male patients (median age: 22.5 years; range, 20 to 24 years) who underwent surgery during which the technique of DUA ulnar bone graft was utilized. One patient with scaphoid nonunion, three patients with Kienböck's disease, and two patients with a traumatic metacarpal defect were retrospectively evaluated. The joint range of motion (ROM), grip strength, Disabilities of the arm, shoulder and Hand (DASH) questionnaire score and Visual Analog Scale (VAS) score, and radiographies before and after surgery were examined. Scintigraphy was performed at 12 weeks postoperatively to monitor the viability of the bone graft. RESULTS: All patients showed improvements in the ROM, grip strength, VAS, and DASH scores. According to the radiographic examination, bone union was achieved in all patients and the scintigraphy revealed that vascularization was detected in the bone tissue. CONCLUSION: The advantages of DUA-based vascularized bone graft are good bone quality and quantity and versatility due to its long pedicle. The osteo-fasio-cutaneous DUA flap seems to be effective in the treatment of traumatic metacarpal bone defects accompanied by skin loss. The DUA-based vascularized ulnar bone may be a source for scaphoid and lunate biological bone reconstruction.

Are Skin Fiducials Comparable to Bone Fiducials for Registration When Planning Navigation-assisted Musculoskeletal Tumor Resections in a Cadaveric Simulated Tumor Model?

BACKGROUND: To improve and achieve adequate bony surgical margins, surgeons may consider computer-aided navigation a promising intraoperative tool, currently applied to a relatively few number of patients in whom freehand resections might be challenging. Placing fiducials (markers) in the bone, identifying specific anatomical landmarks, and registering patients for navigated resections are time consuming. To reduce the time both preoperatively and intraoperatively, skin fiducials may offer an efficient and alternative method of navigation registration. QUESTIONS/PURPOSES: (1) Does preoperative navigation using skin fiducials for registration allow the surgeon to achieve margins similar to those from bone fiducial registration in a simulated lower extremity tumor resection model in cadavers? (2) Does the use of preoperative navigation using skin fiducials for registration allow the surgeon to achieve similar bony margins in pelvic resections of simulated tumors as those achieved in long-bone resections using only skin fiducials for navigation in a cadaver model? METHODS: Simulated bone tumor resections were performed in three fresh-frozen cadavers with intact pelvic and lower-extremity anatomy using navigation guidance. We placed 5-cm intraosseous cement simulated bone tumors in the proximal/distal femur (n = 12), and proximal/distal tibia (n = 12) and pelvis (supraacetabular; n = 6). After bone tumor implantation, CT images of the pelvis and lower extremities were obtained. Each planned osseous resection margin was set at 10 mm. Navigation registration was performed for each simulated tumor using bone and skin markers that act as a point of reference (fiducials). The simulated bone tumor was resected based on a resection line that was established with navigation, and the corresponding osseous margins were calculated after resection. These margins were determined by an orthopaedic surgeon who was blinded to resection planning by the removal of cancellous bone around the cement simulated tumor. The shortest distance was measured from the cement to the resection line. Smaller mean differences between planned and postoperative margins were considered accurate. Independent t-tests were conducted to assess measurement differences between planned and postoperative margins at the 95% CI. Bland-Altman analyses were conducted to compare the deviation in margin difference between planned and postoperative margins in skin and bone fiducial registration, respectively. RESULTS: In all, 84 total resection margins were measured with 48 long bone and 20 pelvic obtained with skin fiducials and 16 long bone obtained with bone fiducials. The planned mean margin was 10 mm for all long bone and pelvic resections. We found that skin fiducial and bone fiducial postoperative margins had comparable accuracy when resecting long bones (10 ± 2 mm versus 9 ± 2 mm, mean difference 1 [95% CI 0 to 2]; p = 0.16). Additionally, skin fiducial long bone postoperative margins were comparable in accuracy to pelvic supraacetabular postoperative margins obtained with skin fiducials (10 ± 2 mm versus 11 ± 3 mm, mean difference -1 mm [95% CI -3 to 1]; p = 0.22). When comparing the deviation in margin difference between planned and postoperative margins in skin and bone fiducial registration, 90% (61 of 68) of skin fiducial and 100% (16 of 16) bone fiducial postoperative margins fell within 2 SDs. CONCLUSIONS: In this pilot study, skin fiducial markers were easy to identify on the skin surface of the cadaver model and on CT images used to plan margins. This technique appears to be an accurate way to plan margins in this model, but it needs to be tested thoroughly in patients to determine if it may be a better clinical approach than with bone fiducials. CLINICAL RELEVANCE: The margins obtained using skin fiducials and bone fiducials for registration were similar and comparable in this pilot study with a very small effect size. Boundaries of the simulated tumors were not violated in any resections. Skin fiducials are easier to identify than bone fiducials (anatomic landmarks). If future clinical studies demonstrate that margins obtained using skin fiducials for registration are similar to margins obtained with anatomical landmarks, the use of navigation with skin fiducials instead of bone fiducials may be advantageous. This technique may decrease the surgeon's time used to plan for and localize registration points and offer an alternative registration technique, providing the surgeon with other registration approaches.

Calcifying fibrous tumor: a case report.

Calcifying fibrous tumors are rarely seen and affect mostly children and young adults. A 21-year-old man presented with multiple palpable masses in the area from the right inguinal region to the anteromedial thigh. We performed a diagnostic excisional biopsy. Histopathologically, it was composed of fibroblasts, psammoma bodies, dystrophic calcifications and foci of mononuclear inflammatory cell infiltration in a collagenous dense stroma. We herein reported a case of calcifying fibrous tumor and discussed its clinical and morphological features with regard to the literature.

Bilateral dorsal trans-scaphoid perilunate fracture-dislocation: A case report.

INTRODUCTION: Perilunate dislocations represent one of the most devastating injuries to the carpus. Fortunately, these injuries are relatively rare, constituting approximately 10% of all carpal injuries. One of the problems associated with this injury is the difficulty of its accurate and early recognition. PRESENTATION OF CASE: In this study, an uncommon case of bilateral dorsal trans-scaphoid perilunate fracture-dislocation following trauma has been reported. The injury was missed initially and the patient was subsequently operated after two weeks. Anatomic reduction was achieved by closed reduction. After closed reduction, percutaneous pin fixation of the carpus was performed using Kirschner wires. Finally, the scaphoid was stabilized with a headless screw percutaneously. The same procedure was repeated for the other wrist. This was followed by an uneventful post-operative period, with a satisfactory functional outcome at the two-year follow-up, despite non-union of the scaphoid in one side. DISCUSSION: The case was examined in detail, and compared to the findings in the literature; observations regarding fracture prognosis were also made. Most authors agree that closed reduction is the initial treatment of choice for trans-scaphoid perilunate fracture-dislocations. In addition, treatment often requires intercarpal fixation within the proximal carpal row. CONCLUSION: We believe that closed reduction in these cases should be attempted regarding the potential risks of avascular necrosis and non-union of the affected carpal bones due to open reduction.

Pins and rubber band traction for treatment of comminuted intra-articular fractures in the hand.

PURPOSE: To determine the efficacy of pins and rubber band traction for treatment of comminuted intra-articular fractures in the hand. METHODS: We performed a retrospective study from 1994 to 2013 to evaluate 33 patients in whom pins and rubber band traction was employed. We clinically evaluated the active range of motion of the affected fingers after surgery. Eleven of the 33 fractures were at the proximal interphalangeal joint, 10 at the distal interphalangeal joint, 5 at the thumb interphalangeal joint, and 2 at the metacarpophalangeal joint of the thumb. The remaining 5 patients had complex fracture-dislocation of the proximal interphalangeal joints. RESULTS: The mean follow-up period was 24 months. The average active motion of the metacarpophalangeal joints of the fingers was 91° (range, extension 0°-10°/flexion 85°-90°), proximal interphalangeal joints was 92° (range, extension/flexion 0°-10°/85°-100°), and distal interphalangeal joints was 73° (range, extension/flexion 0°-10°/60°-80°). The overall average of all active motion of the injured fingers except thumbs was 255° (range, 240°-270°). The average active motion of the of the thumb metacarpophalangeal joint was 56° (range, extension 5°-10°/flexion 50°-55°), and interphalangeal joint was 74° (range, extension 0°-10°/flexion 75°-80°). The average of active motion of the injured thumb metacarpal and interphalangeal joints combined was 130° (range, 125°-135°). CONCLUSIONS: Pins and rubber band traction is a treatment option for comminuted displaced intra-articular fractures of the digits that offers satisfactory clinical results. TYPE OF STUDY/LEVEL OF EVIDENCE: Therapeutic IV.

Consanguineous marriage and increased risk of idiopathic congenital talipes equinovarus: a case-control study in a rural area.

BACKGROUND: The purpose of this study is to evaluate if there is any relationship between consanguineous marriages and idiopathic congenital talipes equinovarus (CTEV). METHODS: A case-control study on CTEV screening was conducted in a rural eastern city of Turkey between 2009 and 2011 and a total of 28 cases (infants with idiopathic CTEV) and 575 controls (healthy infants) were recruited. Sociodemographic status of the infants, including gestational age and birth weights, maternal characteristics and, if any, the degree of consanguinity, were recorded. As an inclusion criterion, only singleton, full-term, live births were accepted. A backward stepwise logistic regression model was used to evaluate the relationship between idiopathic CTEV and parental consanguinity. Unadjusted and adjusted odds ratios (OR) with 95% confidence interval (CI) were calculated. RESULTS: Among maternal and infant characteristics, significant risk factors for idiopathic CTEV in the regression analysis were work status (employed), consanguineous marriage, sex (male), and gestational age (>42 wk). Babies born to first-cousin parents had >4 times the risk of idiopathic CTEV [OR, 4.138, (95% CI, 1.484, 11.538)] and the risk for those born to distant relatives was 2.9 times higher [OR, 2.941, (95% CI, 1.070, 8.087)] than for children of unrelated parents. CONCLUSIONS: Consanguineous marriage was significantly associated with an increased risk of idiopathic CTEV. This association remained significant even after adjusting for potential confounding variables. To obtain more accurate results, a population-based screening study with an increased number of cases and controls should be performed in future studies. LEVEL OF EVIDENCE: Case-control study investigating the effect of a patient characteristic on the outcome of disease (level-III).

Lower limb salvage surgery using Ilizarov circular external frame for a landmine injury about the knee.

Limb salvage for severe trauma has been replaced amputation as the primary treatment in many trauma centers. However, the long-term outcomes after limb reconstruction or amputation have not been fully evaluated. In this report, we present the treatment results of limb salvage surgery using Ilizarov external circular frame in a male case who had a-22-cm bone loss on the left distal femur and left proximal tibia and large soft tissue defect around the knee due to stepping on a landmine with his knee. The decision to amputate a severely injured limb, being irreversible, is challenging and significantly affects the body image and the patient. Extremity salvage surgery should be considered initially when evaluating patients with high-energy injured limbs at high risk for amputation.

Treatment of chronic lateral instability of the ankle with the Colville technique: a prospective analysis with minimum five years of follow-up.

OBJECTIVES: This study aims to prospectively analyze of the long-term results of the Colville's technique for the treatment of chronic lateral ankle instabilities. PATIENTS AND METHODS: Twenty-eight ankles of 28 male patients (mean age 24.6 years; range 20 to 35 years) which were treated using Colville's technique were evaluated with a mean follow-up of 76.1 months (range 60 to 106 months). Ankle instability, ankle functions and outcomes in the last visit were assessed and statistically compared. Stress radiographs with the TELOS device were repeated at six-months and five-years after surgery and compared with the stability of the uninjured ankle. RESULTS: Twenty-three of the results were excellent and five were good according to the criteria of Chrisman and Snook. All patients returned to normal daily activity levels at an average of eight months following surgery. Radiographic analysis revealed the significant preservation of stability at least five years after surgery with no sign of arthritis. The difference between preoperative and sixth-months postoperative values of both the talar tilt and the anterior draw tests were found statistically significant, indicating significant correction of the laxity. On contrary, difference between sixth months and five year values were not statistically significant, indicating the preservation of the correction. CONCLUSION: Anatomical augmented reconstruction procedure of Colville's is a long-lasting and good alternative compared to other more complex techniques of reconstruction with minimum long-term complications.

Periosteal chondroma that presented as a subcutaneous mass in the ring finger.

We present an unusual case of periosteal chondroma of the middle phalanx in the right ring finger that presented as a subcutaneous mass in a 21-year-old man. It was treated by marginal excision, with no postoperative local recurrence.

Correction of syndactyly using a dorsal separated V-Y advancement flap and a volar triangular flap in adults.

Skin grafts and local flaps are conventional methods of repair for congenital syndactyly, but the results obtained are not always as functional and aesthetic as desired and frequently leave postoperative scars and residual syndactyly. In this article, we describe a new surgical technique for web reconstruction in the correction of simple, incomplete syndactyly. The technique consists of a dorsal separated V-Y advancement flap and a volar triangular flap to cover the newly created web space, thus avoiding skin graft in this space. In all, 15 web spaces in 10 patients were treated using this method. A follow-up period of 6 months to 2 years showed neither recurrence of the deformity nor web creep of any degree. The technique is rapid, safe, easily performed, and reproducible and requires a single surgical procedure. It uses donor tissue identical in color, texture, and thickness, which renders acceptable cosmesis in cases of simple, incomplete syndactyly, therefore, avoiding the use of skin grafts and resulting postoperative scar contracture in the web space.

Giant solitary osteochondroma arising from the fifth metatarsal bone: a case report.

Accounting for 20% to 50% of all benign forms, solitary osteochondroma is the most common bone tumor. The long bones of the lower extremity are most frequently affected, whereas the small bones of the hands, feet, pelvis, scapula, and spine are less common locations. Osteochondromas are benign osseous neoplasms with a distinct hyaline cartilage cap originating from the physis, and they cease to grow with skeletal maturity. Treatment of osteochondroma is usually conservative, unless symptoms, usually pain, are progressive or the lesion demonstrates rapid or new growth, or if enlargement after skeletal maturation is noted or malignant transformation is suspected. In this report, we describe the case of an adult with a giant, symptomatic osteochondroma localized to the fifth metatarsal. The lesion was treated with excision, and after more than 2 years of follow-up, no evidence of recurrence was noted. This case demonstrated that, despite the benign nature of the lesion, a large osteochondroma could localize to a metatarsal.

Treatment of a unicameral bone cyst of calcaneus with endoscopic curettage and percutaneous filling with corticocancellous allograft.

The surgical procedures for unicameral solitary calcaneal bone cysts have ranged from simple curettage and grafting to subperiosteal resection with internal fixation and grafting. In this article, an endoscopically assisted technique is proposed for the curettage of a simple calcaneal cyst that takes advantage of direct visualization of the cyst wall and contents and permits accurate assessment of the extent of the lesion. After curettage, percutaneous filling of the defect with corticocancellous allograft makes the technique a complete, minimally invasive surgical approach for this condition. The technique uses 2 lateral portals, one for viewing and the other for manipulation, both of which are created under fluoroscopic control. Once the cyst has been located, the 30 degrees arthroscope is used to evacuate fluid, after which more solid cyst contents are fragmented and removed. Thereafter, curettage of the inner surface of the cavernous cyst wall is performed. Finally, complete packing of the previously cystic cavity with crushed corticocancellous allograft is performed under endoscopic visualization and confirmed radiographically.

Melorheostosis in the upper extremity.

Melorheostosis is a rare mesodermal disease affecting the skeleton and adjacent soft tissues. Often it is incidentally detected on radiographs. In the standard radiology and orthopedics literature, melorheostosis is described as a "flowing hyperostosis, resembling dripping candle wax as an incidental radiographic finding." A 22-year-old man presented with a 2-year history of right-hand pain. Radiologic evaluation of the hand showed massive sclerotic changes in the first and second metacarpal and phalangeal bones on the right side. Further radiographic evaluation of the right upper extremity revealed the same sclerotic changes in the right scapula, humerus, radius, and scaphoid. Computed tomography (CT) scans showed a high attenuation undulating cortical hyperostosis with a "dripping candle wax appearance" involving the radial and/or dorsal aspects of humerus, radius, scaphoid, and first and second ray bones of the hand. Radionuclide triphasic bone scintigraphy showed diffuse homogenous radiotracer uptake within the entire right upper extremity involving the scapula, humerus, radius, scaphoid, and first and second metacarpals and phalangeal bones of the hand. The patient was followed conservatively, and 1-year follow-up revealed no change in the clinical, laboratory, or radiological findings. The diagnosis of melorheostosis was made on the basis of the characteristic distribution, location, and combined radiographic, CT, and radionuclide imaging features of the abnormalities. Conservative treatment was recommended for the patient. After 26 months of follow-up, despite the persistence of the radiologic findings, the patient is currently well, with no painful symptoms unless he performs forceful exercise.