The return to the Moon and the landing on Mars has emphasized the need for greater attention to the effects of partial gravity on human health. Here, we sought to devise a new type of simulated partial gravity apparatus that could more efficiently and accurately provide a partial gravity environment for rat hindlimbs. The new apparatus uses a pulley system and tail suspension to create the simulated partial gravity of the rat's hind limbs by varying the weight in a balance container attached to the pulley system. An experiment was designed to verify the reliability and stability of the new apparatus. In this experiment, 25 seven-week-old male Wistar Hannover rats were randomly divided into five groups (n = 5 per group): hindlimb full weight-bearing control (1G), sham (1G), and the simulated gravity groups including Mars (3/8G), Moon (1/6G), and interplanetary space (microgravity: µG). The levels of partial gravity experienced by rat hindlimbs in the Mars and Moon groups were provided by a novel simulated partial gravity device. Changes in bone parameters [overall bone mineral density (BMD), trabecular BMD, cortical BMD, cortical bone thickness, minimum moment of area (MMA), and polar moment of area (PMA)] were evaluated using computed tomography in all rats at the proximal, middle, and distal regions of femur and tibia. Reduced gravity led to decreases in bone parameters (overall BMD, trabecular BMD, cortical BMD, MMA, and PMA) in the simulated gravity groups, mainly in distal femur and proximal tibia. The proximal tibia, MMA, and PMA findings indicated greater weakness in the µG group than in the Mars group. The sham group design also excluded the decrease in lower limb bone parameters caused by the suspension attachment of the rat's tail. The new simulated partial gravity apparatus can provide a continuous and stable level of partial gravity. It offers a reliable and valuable model for studying the effects of extraterrestrial gravity environments on humans.
The partial gravity environment in space can negatively affect bone health. This survey aimed to study the reaction of different parts of the lower limb bones of rats to partial gravity and the effects of different degrees of gravity on these bony parts. We used 15 8-week-old male Wistar Hannover rats were used at the beginning of the experiment. The degree of mechanical stress was modified, but the ankle joint was maintained at â¼30°, â¼120°, or â¼160° with or without plaster fixation during 10-day hindlimb suspension. Computed tomography was performed to measure the bone parameters [bone mineral density (BMD), trabecular BMD, cortical BMD, and cortical thickness] of each studied group of the whole, proximal, middle, and distal femur and distal tibia. BMD, trabecular BMD, and cortical thickness of the distal femur and proximal tibia of the simulated mechanical stress associated with partial gravity groups were significantly lower than those of the control group; the effect of different degrees of gravity on the same area of hindlimb bone had no significant difference. The simulated mechanical stress associated with partial gravity had the most significant effect on the bone close to the knee joint, with the largest weight-bearing response.
Deep space exploration by humans has become more realistic, with planned returns to the Moon, travel to Mars, and beyond. Space radiation with a low dose rate would be a constant risk for space travelers. The combined effects of space radiation and partial gravity such as on the Moon and Mars are unknown. The difficulty for such research is that there are no good simulating systems on the ground to investigate these combined effects. To address this knowledge gap, we developed the Simulator of the environments on the Moon and Mars with Neutron irradiation and Gravity change (SwiNG) for in vitro experiments using disposable closed cell culture chambers. The device simulates partial gravity using a centrifuge in a three-dimensional clinostat. Six samples are exposed at once to neutrons at a low dose rate (1 mGy/day) using Californium-252 in the center of the centrifuge. The system is compact including two SwiNG devices in the incubator, one with and one without radiation source, with a cooling function. This simulator is highly convenient for ground-based biological experiments because of limited access to spaceflight experiments. SwiNG can contribute significantly to research on the combined effects of space radiation and partial gravity.
