ABSTRACT
Estudiar la asociación entre diferentes elementos morfológicos con aspectos inmunohistoquímicos y su posible utilidad pronóstica. Estudio descriptivo-retrospectivo, de 651 casos de carcinoma mamario con marcaje inmunohistoquímico en el Hospital Vargas de Caracas desde mayo 2001 a julio 2004. Se analizaron características morfológicas de los diferentes tipos tumorales, aplicando la clasificación de Scarff-Bloom-Richardson modificada, relacionándolos con la expresión de diferentes marcadores inmunohistoquímicos: receptores de estrógeno, receptores de progesterona, c-erbB-2 y Ki-67, realizados mediante la técnica de biotina-estreptavidina. La edad promedio fue 51,72 años. El tumor más frecuente fue el carcinoma ductal infiltrante (88,7 por ciento), seguido del carcinoma lobulillar infiltrante (4,6 por ciento). El 5,5 por ciento de los carcinomas ductales eran bien diferenciados, 42,3 por ciento moderadamente diferenciados, 52,2 por ciento poco diferenciados; de estos, el 77 por ciento tenían receptores de estrógeno y de progesterona ≤ 10 por ciento; los carcinomas bien diferenciados mostraron positividad variable. Los tumores poco diferenciados presentaron c-erbB-2 positivo en 51,36 por ciento, entre los bien diferenciados el 81,84 por ciento fueron negativos, el carcinoma intraductal fue positivo en un 42,86 por ciento, generalmente asociado a comedocarcinoma. El 94,37 por ciento de los tumores poco diferenciados fueron Ki-67 positivos. El carcinoma ductal infiltrante de tipo clásico es el tumor maligno más frecuente de la glándula mamaria, con una edad promedio de 51,5 años, generalmente es poco diferenciado, implicando posiblemente tumores con conducta biológica agresiva. Esto se evidencia por la negatividad para receptores hormonales y la expresión aumentada de c-erbB-2 y Ki-67.
To study and assess the association between different morfhological and pathologic features and immunohistochemistry as a prognostic factor. A retrospective-descriptive study of 651 cases of breast cancer by immunohistochemistry markers was analyzed in the Vargas Hospital of Caracas since may 2001 until july 2004. We evaluate the pathologic features of the different tumor subtypes, and relation this with the expression of immunohistochemistry markers: the estrogen receptor, progesterone receptor, c-erbB-2 and Ki-67, made by the biotin-streptavidin technique. The median patient age was 51.72 years. The most frequent type of tumor was the invasive duct carcinoma (88.7 %), follow by the invasive lobular carcinoma (4.6 %). The 5.5 % of the duct carcinomas were well differentiated tumors, 42.3 % moderate differentiated, and 52.2 % poorly differentiated; in which 77 % had estrogen receptor and progesterone receptor ≤ 10 %, well differentiated tumors show a variable positivity. The poorly differentiated tumors show positive c-erbB-2 in 51.36 %, in the well-differentiated group 81.84 % were negative; the c-erbB-2 on intraductal carcinoma was positive in 42.86 %, generally associated with the comedocarcinoma; the 94.7 % of the poorly differentiated tumors were Ki-67 positives. Classic invasive duct carcinoma is the most frequent malignant tumor, with a median age of 51.5 years, and the poorly differentiated tumors generally show an aggressive biologic conduct, associated to patients with estrogen receptors and progesterone receptors negative tumors and a high expression of the c-erbB-2 y Ki-67.
Subject(s)
Humans , Male , Female , Middle Aged , Antigens, Differentiation/immunology , Antigens, Differentiation/chemistry , Neoplasms, Ductal, Lobular, and Medullary/immunology , Neoplasms, Ductal, Lobular, and Medullary/pathology , Carcinoma, Ductal, Breast/pathology , Immunohistochemistry/methods , Medical OncologyABSTRACT
The expression of differentiation associated high PM Keratin polypeptides of the oral mucosa lesions were studied by immunohistochemical and immunoblotting techniques applied to adjacent sections of each biopsy specimen. The material studied included specimens of leukoplakia, verrucous carcinoma, squamous cell carcinoma, adenocarcinoma and keratoacanthoma. Little or no expression of 65-67 Kd keratins was evident in squamous cell carcinoma and adenocarcinoma. Hyperkeratotic (both benign and dysplastic) lesions such as verrucous carcinoma, leukoplakia, and keratoacanthoma, showed great variations in the intensity of 65-67 bands and a very irregular immunohistochemical staining pattern. Increased amounts of horny substance was usually accompanied by absence of, or decreased expression of 65-67 Kd keratins, thus indicating a change in the polypeptide composition of the horny layer in pathological conditions of the oral epithelium.