ABSTRACT
BACKGROUND: Filaggrin (FLG), which is formed from profilaggrin protein during epidermal terminal differentiation, is a prerequisite to squame biogenesis and thus for perfect formation of the skin barrier. Yet, the relationship between genetic polymorphisms of FLG and chronic idiopathic urticaria (CIU) has not been investigated. METHODS: The study population consisted of 93 CIU patients and 93 healthy control subjects without a history of allergic, autoimmune or any other systemic disease. Five single nucleotide polymorphisms (SNPs) of FLG were investigated: rs2485518, rs3126065, rs2786680, rs3814300, and rs3814299. RESULTS: For all the investigated polymorphisms, 100% of both CIU patients and control subjects exhibited one given allele and consequently one given genotype as following: A/A genotype for two SNPs, rs3126065 and rs2786680, C/C genotype for two SNPs, rs2485518 and rs3814300, and G/G genotype for one SNP rs3814299 of FLG, and hence no association was found between either allele frequencies or genotype distributions of FLG SNPs and CIU in an Iranian population. CONCLUSIONS: The present study examined the possible relationship between SNPs of FLG and CIU for the first time, and demonstrated that none of five investigated SNPs (rs2485518, rs3126065, rs2786680, rs3814300, and rs3814299) are correlated with CIU in an Iranian population. Further investigations are required to address whether ethnicity/race impacts on relationship between SNPs of FLG and CIU
No disponible
Subject(s)
Humans , Male , Female , Urticaria/etiology , Urticaria/immunology , Urticaria/pathology , Polymorphism, Genetic/immunology , Autoimmunity , Autoimmunity/immunology , Epidermis/abnormalities , Epidermis/chemistry , Polymerase Chain Reaction/methodsABSTRACT
Diflubenzuron (DFB) is used to control ectoparasitic infestation by inhibiting larvae development in the manure and feces of treated animals. It is also currently been used to control tick infestations. In this study, milk and tissues from cattle treated orally with DFB for a 77-120 day period with a commercial product containing the compound were analyzed for the presence of residues. DFB residues were determined by using extraction with acetonitrile, cleanup with C18 SPE and chromatographic analysis by HPLC with UV detection (254nm). DFB was not detected in any of the analysed samples (<0.006mg kg-1 for fat, <0.014mg kg-1 for muscle, <0.015mg kg-1 for kidney, <0.016mg kg-1 for liver and <0.0006mg kg-1 for milk). In this manner, the use of this compound, according to the manufacturer's suggested doses may result in cattle milk, liver, kidneys, fat and muscles being considered safe regarding the presence of DFB residues.
O diflubenzuron (DFB) é um inibidor de desenvolvimento de insetos que inibe a síntese de quitina com atividade ovicida e larvicida e está sendo utilizado na pecuária para o controle do carrapato. Leite e tecidos provenientes de bovinos tratados por um período de 77 a 120 dias com um produto comercial contendo DFB foram analisados quanto à presença de resíduos. Os resíduos de DFB foram determinados utilizando-se extração com acetonitrila, limpeza por SPE C18 e cromatografia líquida de alta eficiência com detecção por UV (254nm). DFB não foi detectado em nenhuma das amostras analisadas (<0.006mg kg-1 para gordura, <0,014mg kg-1 para músculo, <0,015mg kg-1 para rim, <0,016mg kg-1 para fígado e <0.0006mg kg-1 para leite). Dessa forma, a utilização do princípio ativo conforme recomendado pelo fabricante e em níveis suficientes para se obter o efeito larvicida desejado deve resultar em leite, fígado, rins, gordura e músculos que podem ser considerados seguros para o consumo em termos da presença DFB.
Subject(s)
Animals , Cattle , Diflubenzuron/administration & dosage , Diflubenzuron/chemistry , Diflubenzuron/chemical synthesis , Epidermis/abnormalities , Epidermis , Insecta/cytology , Insecta/chemistryABSTRACT
Se presenta el caso de un varón de 37 años con un cuadro de epidimitis que, tras instaurar tratamiento con norfloxacino, desarrolla lesiones purpúricas en miembros inferiores acompañadas de molestias abdominales. Se describen los pasos que se siguieron en el diagnóstico, evolución y tratamiento, en una consulta de medicina de familia. La aparición de lesiones purpúricas es un importante desafío diagnóstico para los médicos de Atención Primaria. Múltiples procesos patológicos pueden ser responsables de ellas, algunos con graves repercusiones para la salud. Es necesario realizar una completa exploración física y unas pruebas complementarias básicas para poder llegar al diagnóstico y valorar la posible afectación en diferentes órganos. Desde Atención Primaria de Salud, mediante la realización de biopsia-punch y estudio de anatomía patológica, se puede conseguir una adecuada atención de los casos más leves, sin necesidad de derivar a otros niveles asistenciales (AU)
We present the case of a 37-year old man with epididymitis who developed purpura on his legs accompanied by abdominal discomfort after treatment with norfloxacin. The steps followed in a Family Medicine consultation in the diagnosis, his course and treatment are described. Appearance of purpura is a major challenge for Primary Care physicians. Multiple disease conditions may be involved, some with serious health implications. A complete physical examination and some basic complementary tests are required in order to reach the diagnosis and to assess the possible involvement of different organs. Adequate care in the mildest cases, without need for referral to other care levels, can be achieved in Primary Health Care, using a punch biopsy and study of anatomy pathological (AU)
Subject(s)
Humans , Male , Epidermis/abnormalities , Exanthema/blood , Family Practice , Primary Health Care , Migraine with Aura/pathology , Asthma/metabolism , IgA Vasculitis/pathology , Therapeutics/instrumentation , Epidermis , Exanthema/complications , Family Practice/methods , Primary Health Care/methods , Migraine with Aura/metabolism , Asthma/prevention & control , IgA Vasculitis/genetics , Therapeutics/methodsABSTRACT
La aplasia cutánea congénita (ACC) es una rara alteración caracterizada por la ausencia congénita de epidermis, dermis y, en ocasiones, de los tejidos subyacentes. La forma más frecuente afecta al vértex, y se puede presentar de forma aislada o asociada a otras malformaciones. Fue descrita por primera vez por Cordon, en 1767, y desde entonces se han documentado más de 500 casos. La frecuencia se ha estimado en torno a 3/10.000 recién nacidos. No hay predilección por la raza ni el sexo. La etiopatogenia no está clara. La manifestación clínica habitual es una lesión oval o circular, solitaria, sin pelo, bien delimitada, de 0,5-2 cm, y localizada en el vértex. Al nacer, las lesiones pueden estar cicatrizadas o presentarse como erosiones o incluso úlceras profundas, con riesgo de afectar a las meninges o la duramadre. La mejor opción terapéutica dependerá del tamaño del defecto
Aplasia cutis congenita (ACC) is a rare condition characterized by the congenital absence of epidermis, dermis and, in some cases, subcutaneous tissues. ACC occurs as an isolated defect (normally localized agenesis of the scalp) or with other associated anomalies. It was first described by Cordon in 1767, and more than 500 cases have been reported since, with an estimated incidence of 3 cases per 10,000 births. There is no racial or sexual predilection. The pathogenesis is not clear. The lesion is usually solitary, circular or oval, hairless and welldemarcated, and it ranges in size from 0.5-2 cm. At birth, the lesions may have already healed with scarring or may appear as superficial erosions or deep ulcerations, occasionally invo lving the dura or the meninges. The best therapeutic option depends on the size of the defect
Subject(s)
Male , Child , Humans , Ectodermal Dysplasia/diagnosis , Ectodermal Dysplasia/therapy , Alopecia/etiology , Epidermis/abnormalities , Surgical FlapsABSTRACT
No disponible
Subject(s)
Pregnancy , Adult , Female , Humans , Ectodermal Dysplasia/complications , Ectodermal Dysplasia/diagnosis , Ectodermal Dysplasia/therapy , Anti-Infective Agents, Local/therapeutic use , Epidermolysis Bullosa/complications , Epidermolysis Bullosa/diagnosis , Skin Diseases, Genetic/complications , Skin Diseases, Genetic/diagnosis , Epidermis/abnormalities , Epidermis/pathology , Dermis/abnormalities , Dermis/pathologySubject(s)
Humans , Adenocarcinoma, Sebaceous/diagnosis , Adenocarcinoma, Sebaceous/physiopathology , Adenocarcinoma, Sebaceous/rehabilitation , Carcinoma/diagnosis , Carcinoma/physiopathology , Carcinoma/rehabilitation , Lupus Erythematosus, Systemic/diagnosis , Lupus Erythematosus, Systemic/physiopathology , Lupus Erythematosus, Systemic/rehabilitation , Epidermis/abnormalities , Epidermis/physiopathology , Epidermis/injuries , Epidermodysplasia Verruciformis/diagnosis , Epidermodysplasia Verruciformis/physiopathology , Epidermodysplasia Verruciformis/rehabilitation , Fibroma, Ossifying/diagnosis , Fibroma, Ossifying/physiopathology , Fibroma, Ossifying/rehabilitationABSTRACT
Using 28 specimens of clinically normal skin from lepromatous leprosy subjects as a standard for comparison, the mean thickness of the nucleated epidermis was found to be significantly increased in untreated lesions from 16 borderline tuberculoid, 21 erythema nodosum leprosum (ENL), and 14 reversal reaction patients, but was unchanged in borderline lepromatous and lepromatous patients. Using specimens from 36 untreated lepromatous and borderline lepromatous lesions as the standard for comparison with the lesions of reversal reactions or ENL which these patients eventually developed, there was a significant thickening of the nucleated epidermis in both reactional states. In both comparison groups, there was a greater mean increase and a larger frequency of thickening in the ENL lesions than in those with reversal reactions. In the borderline tuberculoid and reversal reaction lesions the increase can be understood as secondary to the presence of gamma interferon or interleukin-2. The increase in thickness in the ENL lesions is more difficult to explain, but it is not inconsistent with a role for these same two cytokines.
Subject(s)
Humans , Epidermis/abnormalities , Epidermis/injuries , Leprosy/surgery , Leprosy/physiopathologyABSTRACT
La técnica de cultivo de queratinocitos puede considerarse como un arma irreemplazable y una alternativa confiable para ser aplicada a pacientes con daño parcial o externo de su piel que ameriten sus propias células especializadas para su recuperación. El objetivo de este estudio fue desarrollar una metodología propia para obtener monocapas de epidermis, para lo cual se obtuvieron muestras de piel procedentes de 7 pacientes sometidos a diferentes procedimientos de cirugía estética. Mediante la dispasa tipo II (2,4 unid/ml) se logró una excelente separación de la epidermis de la dermis al obtener una lámina de epidermis libre de fibroblastos, evidenciado además en los cultivos primarios de las células epidermales, donde se observó el crecimiento celular de los queratinocitos libres de células dérmicas, cuando se cultivaron en el substrato de colágeno tipo I obtenido partir de cola de la rata. En los subcultivos se hizo una comparación de diferentes subtratos-plásticos, fibroblastos inhibidos con mitomicina C (4 Mg/ml), substratos de colágeno y se observó un comportamiento similar de agregación y formación de monocapas entre 8-14 días de cultivo, a excepción de los queratinocitos subcultivos sobre plástico donde las células degeneraron en un tiempo corto. También se analizaron subcultivos en presencia de geles de colágeno tipo I con medios condicionados y se apreció una mayor velocidad de crecimiento en los queratinocitos al igual que cuando se utilizaron fibroblastos inactivados. La obtención de monocapas de células epidermicas se logró entre los 27 y 35 dias después de haberse iniciado el cultivo, mediante hormona de crecimiento a una concentración de 2 Mg/ml; se demostró diferenciación y estratificación de las monocapas de queratinocitos obtenidas in vitro
Subject(s)
Humans , Dermis/abnormalities , Epidermis/abnormalities , Keratosis/surgery , Skin/pathologySubject(s)
Humans , Dermatitis, Atopic/complications , Dermatitis, Atopic/diagnosis , Dermatitis, Atopic/rehabilitation , Sebaceous Gland Diseases/diagnosis , Sebaceous Gland Diseases/physiopathology , Sebaceous Gland Diseases/rehabilitation , Skin Neoplasms/diagnosis , Skin Neoplasms/physiopathology , Skin Neoplasms/rehabilitation , Urticaria/complications , Epidermis/abnormalities , Epidermis/physiology , Epidermis/physiopathology , Myxoma virus/growth & development , Myxoma virus/physiology , Myxoma virus/ultrastructureABSTRACT
Se realiza estudios histopatológicos en 40 pacientes poratdores de dermatosis clinicamente sospechosas de ser procesos neoplásicos malignos de piel. Entran en el estudio pacientes de ambos sexos, sin limitación de otros parámetros. Se observa que predomina el Carcinoma Basocelular siguiéndole en frecuencia el Carcinoma Epidermoide, con predominio de ubicación facial, mayor número de pacientes con pieles tipo II y leve predominio en el sexo femenino. Estudio realizado en un periodo de 4 años en el COnsultorio de Dermatología de la Policlínica Oruro de la ciudad de Oruro, Bolivia del 1ro. de abril de 1990 al 31 de marzo de 1994.
Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Skin Neoplasms/epidemiology , Bolivia/epidemiology , Carcinoma, Basal Cell/epidemiology , Epidermis/abnormalities , Epidermis/physiopathology , Neoplasms, Squamous Cell/etiology , Medical Oncology/trends , Skin Diseases/physiopathology , Skin/injuries , Skin/physiopathologyABSTRACT
Se estudiaron 29 melanomas,en forma prospectiva en relacion a la exprecion del antigeno de proliferacion nuclear(PCNA)indicador de proliferacion celular y del receptor del factor de crecimiento epidermico(EGFR) responsable de estimular la hiperplasia.La PCNA mostro valores elevados y se correlaciono con los parametros habituales.El EGFR con bajo nivel de expresion,presento en el seguimiento un llamativo incremento en los niveles de invasion mas agresivos(nivel V)y en los casos del grupo de"fallecidos".AU
Subject(s)
Receptors, Colony-Stimulating Factor/immunology , Melanoma/diagnosis , Melanoma/pathology , Prognosis , Antigens, Differentiation/history , Epidermis/abnormalities , Epidermis/growth & development , Epidermis/pathology , Skin Neoplasms/classification , Skin Neoplasms/ultrastructureABSTRACT
Se estudiaron 29 melanomas,en forma prospectiva en relacion a la exprecion del antigeno de proliferacion nuclear(PCNA)indicador de proliferacion celular y del receptor del factor de crecimiento epidermico(EGFR) responsable de estimular la hiperplasia.La PCNA mostro valores elevados y se correlaciono con los parametros habituales.El EGFR con bajo nivel de expresion,presento en el seguimiento un llamativo incremento en los niveles de invasion mas agresivos(nivel V)y en los casos del grupo de"fallecidos".
Subject(s)
Antigens, Differentiation/history , Epidermis/abnormalities , Epidermis/growth & development , Epidermis/pathology , Melanoma/diagnosis , Melanoma/pathology , Prognosis , Receptors, Colony-Stimulating Factor/immunology , Skin Neoplasms/classification , Skin Neoplasms/ultrastructureSubject(s)
Child , Adolescent , Dermatitis, Allergic Contact , Dermatitis, Atopic , Dermatitis, Irritant , Dermatitis, Seborrheic , Diaper Rash , Dermatitis, Contact , Dermatology , Sebaceous Glands/abnormalities , Lichen Planus , Skin/anatomy & histology , Carcinoma, Basal Cell , Keratoderma, Palmoplantar , Keratosis , Cutis Laxa , Skin Diseases, Papulosquamous , Skin Diseases/genetics , Darier Disease , Epidermis/abnormalities , Hypersensitivity , Ichthyosis Vulgaris , Melanoma , Melanosis , Neoplasms/etiology , Pityriasis Lichenoides , Pityriasis Rubra Pilaris , Pityriasis Rosea , Pseudoxanthoma Elasticum , Psoriasis , Urticaria , VasculitisABSTRACT
"Tokelau" e uma molestia tropical, em que grandes areas do corpo sao cobertas com aneis concentricos e anulares, pardacentos com grande numero de escamas. O rosto e o couro cabeludo, de um modo geral, sao isentos. A mesma, e proveniente de um fungo vegetal, encontrado na epiderme, particularmente na camada cornea. O micelio e abundante, os esporos mais raros. Ele e formado por longos e finos filamentos bifurcados, juntos uns aos outros. Os esporos sao arredondados, altamente refrataveis, variando no tamanho. Para observa-los e aos esporos, as escamas epidermicas, sao raspadas com um objeto cortante e postas em uma lamina microscopica, com uma gota de potassa caustica (10 a 40 por cento). Uma laminula e aplicada com certa pressao para aplanar as escamas. O fungo e estudado melhor com uma lente de imersao, entretanto pode ser visto com lentes comuns a seco. O "tokelau" foi observado pela primeira vez por William Dampier no Arquipelago Malaio. Manson, em 1876, descreveu as principais caracteristicas da dermatose, considerando-a uma micose epidermica, designando-a pelo nome "Tinea imbricata". Roquette Pinto observou a existencia de Mal no Brasil conforme e relatado em seu livro "Rondonia", Estado do Mato Grosso. Isso foi confirmado por Olimpio da Fonseca e outros. A cultura desse fungo foi obtida por muitos autores, como por exemplo Langeron, Sabouraud, Olimpio da Fonseca, etc. Admite-se em geral que o fungo isolado das escamas e o "Trichophyton concentricum", Blanchard 1896. Vicente Grieco observou no Mato Grosso, Alto Xingu, 11 casos de "tokelau", que sao descritos no artigo junto