Organic material as supplementation for substrate in papaya seedlings production / Material orgânico como suplementação para substrato na produção de mudas de mamoeiro

Papaya is among the main fruit species produced in Brazil, representing high socioeconomic importance in productive regions. Changes in production methodologies aim to meet market demands and sector sustainability. In this sense, the use of organic compounds has been successfully used in the production of seedlings. The objective of the study was to evaluate the potential use of different sources of organic material for the composition of substrates used in the production of papaya seedlings, identifying the effects on the emergence and development of plants. Therefore, the treatments were composed of different substrates, namely: T1 - Soil; T2 - 75% soil and 25% chicken litter; T3 - 75% soil and 25% earthworm humus; T4 - 75% soil and 25% tanned cattle manure. When evaluating the characteristics, it was found that substrates composed of the fractions of earthworm humus and cattle manure allowed increases in the characteristics of shoot length, number of leaves, leaf area and stem circumference, while the presence of chicken litter interfered negatively on all the variables studied. It was concluded that organic compounds have the potential to favor the production of papaya seedlings. In this sense, the composition of the substrate alters the emergence and development of papaya seedlings, and the use of a fraction of 25% of earthworm humus allows the formation of seedlings with greater vigor, while the use of chicken litter in this same proportion should be avoided.

O mamão está entre as principais espécies frutíferas produzidas no Brasil, representando elevada importância socioeconômica nas regiões produtivas. As alterações nas metodologias de produção visam atender às demandas do mercado e à sustentabilidade do setor. Nesse sentido, o emprego de compostos orgânicos tem sido implementado com sucesso na produção de mudas. O objetivo do estudo foi o de avaliar o potencial de diferentes fontes de material orgânico para a composição de substratos utilizados na produção de mudas de mamoeiro, identificando os efeitos sobre a emergência e o desenvolvimento das plantas. Para tanto, os tratamentos foram compostos pelos diferentes substratos, sendo eles: T1 - Solo; T2 - 75% solo e 25% cama de aviário; T3 - 75% solo e 25% húmus de minhoca; T4 - 75% solo e 25% esterco bovino curtido. Ao se avaliar as características, obteve-se que substratos compostos pelas frações de húmus de minhoca e esterco bovino possibilitaram incrementos das características de comprimento da parte aérea, número de folhas, área foliar e circunferência do caule, enquanto que a presença da cama de aviário interferiu negativamente sobre todas as variáveis estudadas. Concluiu-se que os compostos orgânicos possuem potencial para favorecer a produção de mudas de mamoeiro. Neste sentido, a composição do substrato altera a emergência e o desenvolvimento das mudas de mamoeiro, sendo que a utilização de uma fração de 25% de húmus de minhoca possibilita a formação de mudas com maior vigor, enquanto a utilização da cama de aviário nessa mesma proporção deve ser evitada.

Ação curativa e protetora de compostos orgânicos e inorgânicos no controle de Microsphaera diffusa em soja / Curative and protective action of organic and inorganic compounds in the control of Microsphaera diffusa in soybean

O objetivo do trabalho foi avaliar o efeito dos produtos acibenzolar-s-metil, fosfito de potássio, silicato de potássio e trifloxistrobina + protioconazole no controle curativo do oídio (Microsphaera diffusa) na soja. O delineamento experimental foi inteiramente casualizado, em esquema unifatorial, contendo cinco tratamentos com quatro repetições. A aplicação dos produtos foi realizada no estádio fenológico V4, no qual as plantas possuíam 5% de severidade da doença. Aos 7, 14 e 21 dias após aplicação dos tratamentos (DAA), foi avaliada a incidência e severidade da doença. O trifloxistrobina + protioconazole reduziu a incidência em 30% e a severidade em 80%, quando comparado ao controle. Também foi avaliado o efeito inibitório dos produtos na produção de conídios in vivo e na sua germinação in vitro. O trifloxistrobina + protioconazole proporcionou a maior redução na produção de conídios e na inibição da germinação. O acibenzolar-s-metil, fosfito de potássio e silicato de potássio também reduziram a germinação de conídios, mas não afetaram a produção. Com base nisso, conclui-se que, o fungicida trifloxistrobina + protioconazole reduziu a produção de conídios e inibiu sua germinação, apresentando efeito curativo e protetor contra oídio em soja e que os indutores de resistência testados, interferem na germinação do patógeno, mas sem efeito significativo na doença quando aplicados...(AU)

The objective of this work was to assess the effect of acibenzolar-s-methyl, potassium phosphite, potassium silicate, and trifloxystrobin + prothioconazole products on curative control of powdery mildew (Microsphaera diffusa) in soybean. The experimental design was completely randomized, in a single factor scheme, containing five treatments with four replications. The products were applied on leaves at the V4 phenological stage, with disease severity of 5%. At 7, 14, and 21 days after the application of the products (DAA), the incidence and severity of the disease were assessed. The trifloxystrobin + prothioconazole reduced by 30% the disease incidence and 80% disease severity compared to the control. The inhibitory effect of the products on conidia production in vivo and its germination in vitro were also assessed. Trifloxystrobin + prothioconazole was more effective in the reduction of both conidia production and germination. Acibenzolar-s-methyl, potassium phosphite, and potassium silicate reduced conidia germination, but neither affected the disease severity nor the conidia production. Based on this, it can be concluded that the fungicide trifloxystrobin + prothioconazole reduced the production of conidia and inhibited its germination, showing curative and protective effect against soybean powdery mildew and that the resistance inducers tested interfered with pathogen...(AU)

Ação curativa e protetora de compostos orgânicos e inorgânicos no controle de Microsphaera diffusa em soja / Curative and protective action of organic and inorganic compounds in the control of Microsphaera diffusa in soybean

O objetivo do trabalho foi avaliar o efeito dos produtos acibenzolar-s-metil, fosfito de potássio, silicato de potássio e trifloxistrobina + protioconazole no controle curativo do oídio (Microsphaera diffusa) na soja. O delineamento experimental foi inteiramente casualizado, em esquema unifatorial, contendo cinco tratamentos com quatro repetições. A aplicação dos produtos foi realizada no estádio fenológico V4, no qual as plantas possuíam 5% de severidade da doença. Aos 7, 14 e 21 dias após aplicação dos tratamentos (DAA), foi avaliada a incidência e severidade da doença. O trifloxistrobina + protioconazole reduziu a incidência em 30% e a severidade em 80%, quando comparado ao controle. Também foi avaliado o efeito inibitório dos produtos na produção de conídios in vivo e na sua germinação in vitro. O trifloxistrobina + protioconazole proporcionou a maior redução na produção de conídios e na inibição da germinação. O acibenzolar-s-metil, fosfito de potássio e silicato de potássio também reduziram a germinação de conídios, mas não afetaram a produção. Com base nisso, conclui-se que, o fungicida trifloxistrobina + protioconazole reduziu a produção de conídios e inibiu sua germinação, apresentando efeito curativo e protetor contra oídio em soja e que os indutores de resistência testados, interferem na germinação do patógeno, mas sem efeito significativo na doença quando aplicados...

The objective of this work was to assess the effect of acibenzolar-s-methyl, potassium phosphite, potassium silicate, and trifloxystrobin + prothioconazole products on curative control of powdery mildew (Microsphaera diffusa) in soybean. The experimental design was completely randomized, in a single factor scheme, containing five treatments with four replications. The products were applied on leaves at the V4 phenological stage, with disease severity of 5%. At 7, 14, and 21 days after the application of the products (DAA), the incidence and severity of the disease were assessed. The trifloxystrobin + prothioconazole reduced by 30% the disease incidence and 80% disease severity compared to the control. The inhibitory effect of the products on conidia production in vivo and its germination in vitro were also assessed. Trifloxystrobin + prothioconazole was more effective in the reduction of both conidia production and germination. Acibenzolar-s-methyl, potassium phosphite, and potassium silicate reduced conidia germination, but neither affected the disease severity nor the conidia production. Based on this, it can be concluded that the fungicide trifloxystrobin + prothioconazole reduced the production of conidia and inhibited its germination, showing curative and protective effect against soybean powdery mildew and that the resistance inducers tested interfered with pathogen...

Different dietary trace mineral sources for broiler breeders and their progenies.

The objective of this study was to evaluate the effect of different trace mineral supplementation sources in the diet of broiler breeders on their performance and on their progenies. In total, 128 Cobb 500 broiler breeders were distributed according to a completely randomized experimental design in 2 experimental treatments. The control group was fed a diet supplemented with inorganic trace minerals (ITM), while the other group was fed a diet supplemented with reduced levels of trace minerals in the organic form. Eggs were collected when breeders were 35, 47, and 53 wk old. Their progeny (450 hatchlings) were divided according to trace mineral supplementation source from the maternal diet, creating 2 treatments with 16 replicates of 15 birds each. Organic trace mineral (OTM) supplementation improved broiler breeder performance, as shown by higher egg production and better eggshell quality of OTM-fed hens compared with those fed ITM. Egg fertility and hatchability were not influenced by the treatments. As to progeny performance, higher weight gain, and consequently, better feed conversion ratio, were obtained in the 41-day-old progenies of OTM-fed breeders, independently of hen age. Maternal diet trace mineral source did not affect broiler carcass, breast meat, or leg yields. The results of the present study show that supplementing broiler breeder diets with organic trace mineral sources enhances the performance of breeders and their progenies.

Meta-analytic study of organic acids as an alternative performance-enhancing feed additive to antibiotics for broiler chickens.

The effect of organic acids as an alternative to antibiotics on the performance of broiler chickens was evaluated by meta-analysis, identifying and quantifying the main factors that influence results. A total of 51,960 broilers from 121 articles published between 1991 and 2016 were used. Interactions of additives [non-supplemented group (control), organic acids, and growth promoter antibiotics] with microbial challenge (with or without inoculation of pathogenic microorganisms) were studied on performance variables. Moreover, the effects of organic acids, used individually or in blends, were evaluated. Relative values of average daily gain (ADG) and average daily feed intake (ADFI) were obtained in relation to control: ΔADG and ΔADFI, respectively. Analysis of variance-covariance revealed lower ADG with organic acids when compared to antibiotics (P < 0.05). There was a significant interaction between the additives and the challenge on feed conversion ratio (FCR) (P < 0.01) and on viability (P < 0.05). Without challenge, organic acids improved broilers' FCR (P < 0.01), presenting results similar to antibiotics (P > 0.05). Under challenge, the organic acids were again effective on FCR (-5.67% in relation to control, P < 0.05), but they did not match antibiotics (-13.40% in relation to control, P < 0.01). Viability was improved only under challenge conditions, and only by antibiotics (+4.39% in relation to control, P < 0.05). ADG (P < 0.05) and FCR (P < 0.01) were increased by blends of organic acids, but not by the organic acids used alone (P > 0.05). ADFI and production factor were not influenced by the treatments (P > 0.05). ΔADFI of organic-acid supplemented group showed a linear influence on ΔADG, which increases 0.64% at every 1% increase in ΔADFI. In conclusion, organic acids can be utilized as performance enhancing, but the results are lower than those found with antibiotics, particularly under microbial challenge. The blends of organic acids provide better results than the utilization of one organic acid alone.

Effects of 4-Nonylphenol on reproduction of exposed females during puberty

4-Nonylphenol (NP) is considered an endocrine disruptor due to its capacity to interact with endocrine system, mimicking estrogen activity. It is aubiquitous pollutant since it has been found in many human tissue samples and its precursor identified in sewage, sediments, rivers and drinking water. Thus, this work aimed to evaluate reproductive parameters in female Balb/C mice treated with 50 mg/kg body weight of NP for 21 days, a period in which they reach sexual maturity. Body weight during treatment, day of vaginal opening, organs weight, fertility rate and proportion of different follicle types were some of these parameters. Additionally, for analysis of some effect in littermates, a group of animals underwent two attempts at pregnancy after treatment. NP had no effect on body weight during treatment, nor did it alter the day of vaginal opening and fertility rate of females. The proportion of preantral, antral and degenerated ovarian follicles was not modified by treatment with NP, and no morphological or pathological alterations were observed. The number of pups did not differ between treatments in any attempt at pregnancy. Also the weight of pups on birth and female rate in littermates was similar among experimental groups after both trials of pregnancy. It is suggested that the NP available or accumulated during the period of sexual maturation (21 days) did not reach threshold levels capable of producing significant alterations in the reproductive parameters analyzed.(AU)

Effects of 4-Nonylphenol on reproduction of exposed females during puberty

4-Nonylphenol (NP) is considered an endocrine disruptor due to its capacity to interact with endocrine system, mimicking estrogen activity. It is aubiquitous pollutant since it has been found in many human tissue samples and its precursor identified in sewage, sediments, rivers and drinking water. Thus, this work aimed to evaluate reproductive parameters in female Balb/C mice treated with 50 mg/kg body weight of NP for 21 days, a period in which they reach sexual maturity. Body weight during treatment, day of vaginal opening, organs weight, fertility rate and proportion of different follicle types were some of these parameters. Additionally, for analysis of some effect in littermates, a group of animals underwent two attempts at pregnancy after treatment. NP had no effect on body weight during treatment, nor did it alter the day of vaginal opening and fertility rate of females. The proportion of preantral, antral and degenerated ovarian follicles was not modified by treatment with NP, and no morphological or pathological alterations were observed. The number of pups did not differ between treatments in any attempt at pregnancy. Also the weight of pups on birth and female rate in littermates was similar among experimental groups after both trials of pregnancy. It is suggested that the NP available or accumulated during the period of sexual maturation (21 days) did not reach threshold levels capable of producing significant alterations in the reproductive parameters analyzed.

La fibra alimentaria y su uso terapéutico en algunas enfermedades crónicas / The alimentary fiber and their therapeutic use in some chronic diseases

La fibra dietética o fibra alimentaria, forma parte de lo que se considera una dieta equilibrada o saludable. A pesar que la fibra alimentaria no se considera un nutriente, es un componente importante de la dieta diaria. La razón principal de su importancia, es que pasa por el sistema digestivo sin ser absorbida y este hecho fisiológico, trae beneficios a la salud. La fibra alimentaria, se considera como el material alimenticio particularmente de origen vegetal que no es hidrolizado por las enzimas secretadas por el tracto digestivo humano, pero, que puede ser fermentada en el intestino grueso por la microflora colónica. El grupo de enfermedades crónicas no transmisibles, incluye: obesidad, diabetes, enfermedades cardiovasculares y cerebrovasculares, la hipertensión arterial, el cáncer y problemas articulares. Estudios epidemiológicos, muestran el efecto beneficioso de la fibra en el tratamiento terapéutico en algunas de estas enfermedades crónicas

Dietary fiber or alimentary fiber, is part of what is considered a balanced or healthy diet. Although the alimentary fiber is not considered a nutrient, is an important component of the diet. The main reason for its importance is passing through the digestive system without being absorbed and this physiological fact is beneficial to health. The alimentary fiber is considered as particularly the food material of plant origin that is not hydrolyzed by enzymes secreted by the human digestive tract, but that can be fermented in the large intestine by colonic microflora. The group of chronic non-communicable diseases, include: obesity, diabetes, cardiovascular and cerebrovascular diseases, hypertension, cancer and joint problems. Epidemiological studies show the beneficial effect of fiber in the therapeutic treatment in some of these chronic diseases

Produção de biomassa de erva-cidreira [Lippia alba (Mill. ) N.E.Br. ] sob adubação com composto de capim elefante inoculado e sem inoculação de actinomicetos / Biomass production of Lippia alba (Mill. ) N.E.Br. under fertilization with compost inoculated elephant grass and without inoculation of actinomycetes

A erva-cidreira [Lippia alba (Mill.) N. E. Br.] é uma espécie da família Verbenaceae amplamente utilizada pela população brasileira, devido às propriedades calmante, espasmolítica suave, analgésica, sedativa, ansiolítica e levemente expectorante. Para implantação do experimento foi produzido composto orgânico de capim elefante + esterco, na proporção de 70% de material vegetal e 30% de esterco, sem inoculação e inoculados com os isolados de actinomicetos do gênero Streptomyces: AC16; AC26; AC92 e AC103. Os compostos foram acondicionados em sacos de polietileno contendo 10,0 kg de solo adicionados na proporção equivalente a 20 t ha-1 e foram reaplicados logo após a primeira colheita. As colheitas da parte aérea foram realizadas aos 75 e 165 dias após o transplante. As raízes foram colhidas logo após a segunda colheita. Os tratatamentos com adubação orgânica promoveram aumento médio na produção de biomassa da erva-cidreira de 184, 83, 125, 115 e 122% para biomassa seca da parte aérea (MSPA) na primeira colheita, de MSPA na segunda colheita, biomassa seca total da parte aérea (MSTPA), biomassa seca de raiz (MSR) e biomassa seca total da cultura (MST), respectivamente, quando comparados com o tratamento controle, porém não houve efeito da inoculação de actinomicetos no composto orgânico. Nas condições em que este estudo foi realizado foi possível concluir que a adubação com composto orgânico de capim elefante influenciou positivamente a produção de biomassa seca da L. alba, mas a inoculação dos compostos com actinomicetos não exerceu influência.

L. alba is a species of the family Verbenaceae widely used by the Brazilian population, because of the soothing, gentle antispasmodic, analgesic, expectorant, sedative, anxiolytic and smoothly. For the implementation of the experiment produced organic compound of elephant grass (Pennisetum purpureum Shum.) + manure at 70% plant material and 30% manure, uninoculated and inoculated with the actinomycete Streptomyces genus, AC16, AC26, AC92 and AC103. The compounds were placed in polyethylene bags containing 10.0 kg of soil added in a proportion equivalent to 20 t ha-1 and re-apply after the first harvest. The harvests of the shoots were taken at 75 and 165 days after transplantation. The roots were harvested after the second harvest. Dealing with organic fertilization promoted the average increase in biomass production of lemon grass 184, 83, 125, 115 and 122% for the dry stem biomass (MSPA) in the first harvest, second harvest of MSPA, total shoot biomass (MSTPA), dry biomass of the root (MSR) and the total crop biomass (MST), respectively, compared with the control, but no effect of inoculation of actinomycetes in compost. Under the conditions of this study was conducted, it was concluded that the organic compound fertilizer with elephant grass positively influenced the production of dry biomass of L. alba, but the injection of the compounds of the actinomycetes, without influence.

Composição química e produtividade dos principais componentes do óleo essencial de Baccharis dracunculifolia DC. em função da adubação orgânica / Chemical composition and productivity of essential oil of Baccharis dracunculifolia DC. as affected by organic compound

Objetivou-se avaliar a composição química e produtividade dos principais componentes do óleo essencial de Baccharis dracunculifolia DC. em função de doses de composto orgânico (0, 10, 20, 30, 40 e 50 t ha-1). Foi realizada uma colheita, aos 150 dias após o transplante das mudas. O óleo essencial, da massa seca útil da parte aérea, foi extraído por hidrodestilação e analisado em cromatógrafo a gás acoplado a espectrômetro de massas (Shimadzu, QP-5000). A identificação dos constituintes químicos foi realizada através da análise comparativa dos espectros de massas das substâncias com o banco de dados do sistema CG-EM (Nist 62.lib), literatura e índice de retenção. Os resultados foram submetidos à análise de variância pelo teste F, às médias obtidas foram submetidas à análise de regressão e o teste Tukey para o efeito das doses de composto orgânico. Os três componentes sesquiterpênicos, E-nerolidol, espatulenol e óxido de cariofileno, perfazem 58,44% da média relativa da composição química do óleo essencial de B. dracunculifolia, composto pela presença de 28 substâncias. Na produtividade dos componentes γ-muroleno, valenceno, δ-cadineno e E-nerolidol as dosagens estudadas influenciaram as plantas, que na dosagem 30 t ha-1 obtiveram os melhores resultados. Se o objetivo no cultivo de B. dracunculifolia for o componente espatulenol as dosagens 30 e 40 t ha-1 obtiveram os melhores resultados. Para a produtividade do componente óxido de cariofileno as dosagens estudadas influenciaram as plantas, que na dosagem 40 t ha-1 obtiveram os melhores resultados.

The objective was to evaluate the chemical composition and productivity of the main components of the essential oil of Baccharis dracunculifolia DC. due to organic compound (0, 10, 20, 30, 40 and 50 t ha-1). We performed a harvest, 150 days after transplanting. The essential oil of the dry mass useful in the aerial part was extracted by hydrodistillation and analyzed in gas chromatograph coupled to a mass spectrometer (Shimadzu, QP-5000). The identification of chemical constituents was performed by comparative analysis of mass spectra of substances with the database of the GC-MS (Nist 62.lib), literature and retention rates. The results were subjected to analysis of variance by F test, the averages obtained were subjected to regression analysis and Tukey test for the effect of doses of organic compound. The three components sesquiterpene, E-nerolidol, espatulenol and caryophyllene oxide, account for 58.44% of the average for the chemical composition of essential oil of B. dracunculifolia, composed by the presence of 28 substances. Productivity of components γ-murolene, valencene, δ-cadinene and E-nerolidol was influenced by doses, which in dose 30 t ha-1 obtained the best results. If the goal in the cultivation of B. dracunculifolia is the component espatulenol dosages 30 and 40 t ha-1 obtained the best results. To the productivity of caryophyllene oxide doses studied influenced the plants, which in dose 40 t ha-1 obtained the best results.

Produção e qualidade do pasto de coastcross consorciado ou não com amendoim forrageiro com ou sem aplicação de nitrogênio / Production and quality of coastcross pasture associated or not with forage peanut with or without nitrogen

Avaliaram-se a taxa de acúmulo foliar (TAF), a produção total de forragem (PTF), a composição química da forragem e os valores de digestibilidade in vitro da matéria seca (DIVMS) do coastcross (Cynodon dactylon) e do amendoim forrageiro (Arachis pintoi) em pastagem consorciada. Foram avaliados os tratamentos (parcelas): coastcross + A. pintoi sem nitrogênio (CA0); coastcross + A. pintoi com 100kg de nitrogênio (CA100); coastcross com 200kg de nitrogênio (C200) e coastcross + A. pintoi com 200kg de nitrogênio (CA200), e as estações (subparcelas) do ano inverno, primavera, verão e outono. Utilizou-se um delineamento experimental em blocos ao acaso, em esquema de parcelas subdivididas no tempo, com duas repetições. Os tratamentos com os maiores níveis de adubação de nitrogênio apresentaram TAF mais alto que os demais (P<0,05). No verão ocorreu a maior PTF, seguida da primavera, outono e inverno, mas estes não diferiram entre si. Não houve diferença (P>0,05) entre os tratamentos para as variáveis proteína bruta, fibra em detergente neutro e digestibilidade in vitro de matéria seca, tanto para as frações de folhas do coastcross, como para a planta inteira do A. pintoi. A adubação nitrogenada, quando aplicada à pastagem de coastcross singular ou associada ao amendoim forrageiro, proporcionou aumento da TAF e da PTF, com maior produtividade e qualidade no verão.(AU)

Forage accumulation rate (FAR), total forage production (TFP), chemical composition and in vitro dry matter digestibility (IVDMD) of coastcross pasture mixed with Arachis pintoi (AP) were evaluated. Treatments plots were: coastcross + A. pintoi without N (CA0); coastcross + A. pintoi with 100kg of N (CA100); coastcross with 200kg of N (C200); and coastcross + A. pintoi with 200kg of N (CA200); and seasons of the year (split-plots): winter, spring, summer, and autumn were analyzed. A randomized block design was used subdivided into time plots, with two replications. Treatments with the highest fertilizer levels presented higher FAR than others (P<0.05), with higher TFP during the summer, followed by spring, autumn, and winter, with no differences among then. There were no differences (P>0.05) among treatments for CP, NDF, and IVDMD on coastcross leaf fractions and Arachis pintoi whole plant. Nitrogen fertilization, when applied to single coastcross pasture or mixed with forage peanut, increased accumulation rate and forage accumulation, with higher productivity and quality in summer.(AU)

Respostas planctônica e bentônica a diferentes fertilizações no cultivo do camarão farfantepenaeus subtilis (Pérez-Farfante, 1967 / Planktonic and Bentonic responses to different fertilizations in the Farfantepenaeus Subtilis (Pérez-Farfante, 1967) Shrimp culture

O presente trabalho objetivou a indução do alimento natural no cultivo do Farfantepenaeus subtilis, através de diferentes regimes de fertilização. Foram utilizados 12 tanques em fibra de vidro de 500 L, estocados com 30 camarões.m-² (2,57±1,27 g), adotando-se quatro tratamentos em triplicata, sendo três com os fertilizantes orgânicos farelo de trigo – FT (28 g.m-²), farelo de arroz – FA (28 g.m-²) e melaço – ML (40 mL.m-²), e um como controle – CT, com fertilizantes inorgânicos à base de nitrogênio (2 mg.L-1) e fósforo (0,2 mg.L-1). A alimentação artificial foi ofertada em comedouros, em três horários, com coletas quinzenais de água, plâncton e bentos. Os resultados demonstraram não haver diferença significativa (P≥0,05) na sobrevivência dos camarões entre os fertilizantes orgânicos, porém, houve diferença estatística (P<0,05) entre FT e CT. Quanto ao alimento natural, não houve diferença significativa (P≥0,05) entre os grupos planctônicos. No fitoplâncton, houve a predominância de cianobactérias, enquanto que no zooplâncton, os principais representantes foram os rotíferos. Entre os organismos bentônicos, o fitobentos também foi representado por cianobactérias, havendo diferença (P<0,05) somente no grupo das euglenas, onde FA diferiu de FT e CT. No zoobentos, registrou-se predominância de rotíferos e nematóides, havendo diferença (P<0,05) no grupo dos rotíferos em ML, quando comparado com CT e FT. Os nematóides diferiram em FA quando relacionado com FT e ML, os quais também predominaram no mesobentos, não havendo diferença estatística (P≥0,05) entre os tratamentos. Desta forma, constata-se que, nas condições experimentais adotadas, o efeito dos fertilizantes orgânicos é similar aos inorgânicos quanto à indução do alimento natural /

This work aimed to evaluate the natural food induction in Farfantepenaeus subtilis culture, through different fertilization regimes. Twelve 500 L fiber glass tanks were stocked with 30 shrimps.m-2 (2.57±1.27 g). Four experimental treatments were adopted in triplicate, using the three organic fertilizers wheat bran – FT (28 g.m-²), rice bran – FA (28 g.m-²) and molasses – ML (40 mL.m-²), and one control – CT, with inorganic fertilizers (2 mg.L-1 nitrogen and 0.2 mg.L-1 phosphorus). Artificial feed was offered in feeding trays three times a day, and plankton, benthos and water samples were collected fortnightly. Results showed no significant difference (P≥0.05) in survival among the organic fertilizers treatments, but showed statistical difference (P<0.05) among FT and CT. About the natural food organisms, there was no significant difference (P≥0.05) among planktonic groups. In the phytoplankton, there was predominance of Cyanophyta, while in the zooplankton the major representants were rotifers. Among the benthos, the phytobenthos also was represented by Cyanophyta, but there was statistical difference (P<0.05) only in Euglena group, where FA was different (P<0.05) from FT and CT. In the zoobenthos, it was registered the predominance of rotifers and nematodes, showing significant difference (P<0.05) in rotifers group in ML, when compared with CT and FT. Nematodes differed (P<0.05) in FA when related to FT and ML, which also predominated in mesobenthos, with no significant difference (P≥0.05) among the treatments. It was concluded that in the experimental conditions adopted both organic and inorganic fertilizers effects were similar related to natural food induction

Respostas planctônica e bentônica a diferentes fertilizações no cultivo do camarão farfantepenaeus subtilis (Pérez-Farfante, 1967 / Planktonic and Bentonic responses to different fertilizations in the Farfantepenaeus Subtilis (Pérez-Farfante, 1967) Shrimp culture

O presente trabalho objetivou a indução do alimento natural no cultivo do Farfantepenaeus subtilis, através de diferentes regimes de fertilização. Foram utilizados 12 tanques em fibra de vidro de 500 L, estocados com 30 camarões.m-² (2,57±1,27 g), adotando-se quatro tratamentos em triplicata, sendo três com os fertilizantes orgânicos farelo de trigo FT (28 g.m-²), farelo de arroz FA (28 g.m-²) e melaço ML (40 mL.m-²), e um como controle CT, com fertilizantes inorgânicos à base de nitrogênio (2 mg.L-1) e fósforo (0,2 mg.L-1). A alimentação artificial foi ofertada em comedouros, em três horários, com coletas quinzenais de água, plâncton e bentos. Os resultados demonstraram não haver diferença significativa (P≥0,05) na sobrevivência dos camarões entre os fertilizantes orgânicos, porém, houve diferença estatística (P<0,05) entre FT e CT. Quanto ao alimento natural, não houve diferença significativa (P≥0,05) entre os grupos planctônicos. No fitoplâncton, houve a predominância de cianobactérias, enquanto que no zooplâncton, os principais representantes foram os rotíferos. Entre os organismos bentônicos, o fitobentos também foi representado por cianobactérias, havendo diferença (P<0,05) somente no grupo das euglenas, onde FA diferiu de FT e CT. No zoobentos, registrou-se predominância de rotíferos e nematóides, havendo diferença (P<0,05) no grupo dos rotíferos em ML, quando comparado com CT e FT. Os nematóides diferiram em FA quando relacionado com FT e ML, os quais também predominaram no mesobentos, não havendo diferença estatística (P≥0,05) entre os tratamentos. Desta forma, constata-se que, nas condições experimentais adotadas, o efeito dos fertilizantes orgânicos é similar aos inorgânicos quanto à indução do alimento natural (AU) /

This work aimed to evaluate the natural food induction in Farfantepenaeus subtilis culture, through different fertilization regimes. Twelve 500 L fiber glass tanks were stocked with 30 shrimps.m-2 (2.57±1.27 g). Four experimental treatments were adopted in triplicate, using the three organic fertilizers wheat bran FT (28 g.m-²), rice bran FA (28 g.m-²) and molasses ML (40 mL.m-²), and one control CT, with inorganic fertilizers (2 mg.L-1 nitrogen and 0.2 mg.L-1 phosphorus). Artificial feed was offered in feeding trays three times a day, and plankton, benthos and water samples were collected fortnightly. Results showed no significant difference (P≥0.05) in survival among the organic fertilizers treatments, but showed statistical difference (P<0.05) among FT and CT. About the natural food organisms, there was no significant difference (P≥0.05) among planktonic groups. In the phytoplankton, there was predominance of Cyanophyta, while in the zooplankton the major representants were rotifers. Among the benthos, the phytobenthos also was represented by Cyanophyta, but there was statistical difference (P<0.05) only in Euglena group, where FA was different (P<0.05) from FT and CT. In the zoobenthos, it was registered the predominance of rotifers and nematodes, showing significant difference (P<0.05) in rotifers group in ML, when compared with CT and FT. Nematodes differed (P<0.05) in FA when related to FT and ML, which also predominated in mesobenthos, with no significant difference (P≥0.05) among the treatments. It was concluded that in the experimental conditions adopted both organic and inorganic fertilizers effects were similar related to natural food induction (AU)

Safety and efficacy of the HIV-1 integrase inhibitor raltegravir (MK-0518) in treatment-experienced patients with multidrug-resistant virus: a phase II randomised controlled trial.

BACKGROUND: Raltegravir (MK-0518) is an HIV-1 integrase inhibitor with potent in-vitro activity against HIV-1 strains including those resistant to currently available antiretroviral drugs. The aim of this study was to assess the safety and efficacy of raltegravir when added to optimised background regimens in HIV-infected patients. METHODS: HIV-infected patients with HIV-1 RNA viral load over 5000 copies per mL, CD4 cell counts over 50 cells per muL, and documented genotypic and phenotypic resistance to at least one nucleoside reverse transcriptase inhibitor, one non-nucleoside reverse transcriptase inhibitor, and one protease inhibitor were randomly assigned to receive raltegravir (200 mg, 400 mg, or 600 mg) or placebo orally twice daily in this multicentre, triple-blind, dose-ranging, randomised study. The primary endpoints were change in viral load from baseline at week 24 and safety. Analyses were done on a modified intention-to-treat basis. This trial is registered with ClinicalTrials.gov, with the number NCT00105157. FINDINGS: 179 patients were eligible for randomisation. 44 patients were randomly assigned to receive 200 mg raltegravir, 45 to receive 400 mg raltegravir, and 45 to receive 600 mg raltegravir; 45 patients were randomly assigned to receive placebo. One patient in the 200 mg group did not receive treatment and was therefore excluded from the analyses. For all groups, the median duration of previous antiretroviral therapy was 9.9 years (range 0.4-17.3 years) and the mean baseline viral load was 4.7 (SD 0.5) log10 copies per mL. Four patients discontinued due to adverse experiences, three (2%) of the 133 patients across all raltegravir groups and one (2%) of the 45 patients on placebo. 41 patients discontinued due to lack of efficacy: 14 (11%) of the 133 patients across all raltegravir groups and 27 (60%) of the 45 patients on placebo. At week 24, mean change in viral load from baseline was -1.80 (95% CI -2.10 to -1.50) log10 copies per mL in the 200 mg group, -1.87 (-2.16 to -1.58) log10 copies per mL in the 400 mg group, -1.84 (-2.10 to -1.58) log10 copies per mL in the 600 mg group, and -0.35 (-0.61 to -0.09) log(10) copies per mL for the placebo group. Raltegravir at all doses showed a safety profile much the same as placebo; there were no dose-related toxicities. INTERPRETATION: In patients with few remaining treatment options, raltegravir at all doses studied provided better viral suppression than placebo when added to an optimised background regimen. The safety profile of raltegravir is comparable with that of placebo at all doses studied.

Peripheral and spinal mechanisms of antinociceptive action of lumiracoxib.

The possible participation of the nitric oxide (NO)-cyclic GMP-K(+) channel pathway, serotonergic or opioidergic system on lumiracoxib-induced local or intrathecal antinociception was assessed in the formalin test. Local or intrathecal administration of lumiracoxib dose-dependently produced antinociception in the second phase of the test. Moreover, local or intrathecal pretreatment with N(G)-L-nitro-arginine methyl ester (L-NAME, NO synthesis inhibitor), 1H-(1,2,4)-oxadiazolo(4,2-a)quinoxalin-1-one (ODQ, guanylyl cyclase inhibitor), glibenclamide (ATP-sensitive K(+) channel blocker), charybdotoxin and apamin (large- and small-conductance Ca(2+)-activated-K(+) channel blockers, respectively) or margatoxin (voltage-dependent K(+) channel blocker), but not N(G)-D-nitro-arginine methyl ester (D-NAME) or vehicle, significantly prevented lumiracoxib-induced antinociception. The intrathecal injection of methiothepin (serotonin receptor antagonist) reduced lumiracoxib-induced intrathecal antinociception. Local peripheral or intrathecal naloxone did not modify either local or intrathecal lumiracoxib-induced antinociception. Results suggest that lumiracoxib activates the NO-cyclic GMP-K(+) channels to produce local and intrathecal antinociception. Data also suggest that lumiracoxib activates the intrathecal serotonergic system, but not opioid receptors either at peripheral or spinal sites.