ABSTRACT
Pectic polysaccharides have long been a challenging subject of research in the field of macromolecular science, given their complex structures and wide range of biological effects. However, the extensive exploration of pectic polysaccharides has been limited due to the intricacy of their structures. In this comprehensive review, we aim to provide a thorough summary of the existing knowledge on pectic polysaccharides, with a particular focus on aspects such as classification, extraction methodologies, structural analysis, elucidation of biological activities, and exploration of target molecules and signaling pathways. By conducting a comprehensive analysis of existing literature and research achievements, we strive to establish a comprehensive and systematic framework that can serve as a reference and guide for further investigations into pectic polysaccharides. Furthermore, this review delves into the applications of pectic polysaccharides beyond their fundamental attributes and characteristics, exploring their potential in fields such as materials, food, and pharmaceuticals. We pay special attention to the promising opportunities for pectic polysaccharides in the pharmaceutical domain and provide an overview of related drug development research. The aim of this review is to facilitate a holistic understanding of pectic polysaccharides by incorporating multifaceted research, providing valuable insights for further in-depth investigations into this significant polymer.
Subject(s)
Pectins , Pectins/chemistry , Pectins/isolation & purification , Humans , Animals , Polysaccharides/chemistry , Polysaccharides/isolation & purification , Polysaccharides/pharmacologyABSTRACT
Oligosaccharides from uronic acid-containing polysaccharides can be produced either by chemical or enzymatic degradation. The benefit of using enzymes, called lyases, is their high specificity for various glycosidic linkages. Lyases cleave the polysaccharide chain by an ß-elimination reaction, yielding oligosaccharides with an unsaturated sugar (4-deoxy-l-erythro-hex-4-enepyranosyluronate) at the non-reducing end. In this work we have systematically studied acid degradation of unsaturated uronic acid oligosaccharides. Based on these findings, a method for preparing saturated oligosaccharides by enzymatic degradation of uronic acid-containing polysaccharides was developed. This results in oligosaccharides with a pre-defined distribution and proportion of sugar residues compared to the products of chemical degradation, while maintaining the chemical structure of the non-reducing end. The described method was demonstrated for generating saturated oligosaccharides of alginate, heparin and polygalacturonic acid. In the case of alginate, the ratio of hydrolysis rate of Δ-G and Δ-M linkages to that of G-G and M-M linkages, respectively, was found to be approximately 65 and 43, at pH* 3.4, 90 °C. Finally, this method has been demonstrated to be superior in the production of α-l-guluronate oligosaccharides with a lower content of ß-d-mannuronate residues compared to what can be achieved using chemical depolymerization alone.
Subject(s)
Alginates , Oligosaccharides , Uronic Acids , Alginates/chemistry , Oligosaccharides/chemistry , Uronic Acids/chemistry , Hydrolysis , Polysaccharide-Lyases/chemistry , Polysaccharide-Lyases/metabolism , Polysaccharides/chemistry , Pectins/chemistry , Heparin/chemistryABSTRACT
This article presents a method for producing hydrogel dressings using high methylated pectin from apples or citrus, doped with the antiseptic agent, octenidine dihydrochloride. Octenidine was incorporated in-situ during the polymer crosslinking. The pectins were characterized by their varying molecular weight characteristics, monosaccharide composition, and degree of esterification (DE). The study assessed the feasibility of producing biologically active hydrogels with pectin and delved into how the polymer's characteristics affect the properties of the resulting dressings. The structure evaluation of hydrogel materials showed interactions between individual components of the system and their dependence on the type of used pectin. Both the antimicrobial properties and cytotoxicity of the dressings were evaluated. The results suggest that the primary determinants of the functional attributes of the hydrogels are the molecular weight characteristics and the DE of the pectin. As these values rise, there is an increase in polymer-polymer interactions, overshadowing polymer-additive interactions. This intensification strengthens the mechanical and thermal stability of the hydrogels and enhances the release of active components into the surrounding environment. Biological evaluations demonstrated the ability of octenidine to be released from the dressings and effectively inhibit the growth of microbial pathogens.
Subject(s)
Anti-Infective Agents, Local , Bandages , Hydrogels , Imines , Pectins , Pyridines , Pectins/chemistry , Pectins/pharmacology , Imines/chemistry , Hydrogels/chemistry , Hydrogels/pharmacology , Anti-Infective Agents, Local/chemistry , Anti-Infective Agents, Local/pharmacology , Pyridines/chemistry , Pyridines/pharmacology , Citrus/chemistry , Malus/chemistry , Molecular Weight , Humans , Microbial Sensitivity Tests , Staphylococcus aureus/drug effects , AnimalsABSTRACT
Purpose: Conventional oral formulations for inflammatory bowel disease (IBD) treatment are less than satisfactory, due to the poor controllability of drug release and lack of specificity to the inflammation sites in the gastrointestinal (GI) tract. To overcome these limitations, we developed a multiple carbohydrate-based nanosystem with pH/ROS dual responsibility and charge-mediated targeting ability for IBD-specific drug delivery. Methods: In view of the overproduction of ROS and overexpression of cationic proteins in the inflammatory colon, the designed nanosystem was composed of oxidation-sensitive cyclodextrin (OX-CD), chitosan (CS) and pectin (AHP). OX-CD was utilized to load dexamethasone (DM) by the solvent evaporation method. CS and AHP with opposite charges were sequentially coated onto OX-CD to generate the nanosystems by the electrostatic self-assembly method. The physicochemical properties, stability, dual-sensitive drug release behavior, cytotoxicity, cellular uptake and anti-inflammatory activity were investigated in vitro. In vivo bio-distribution and therapeutic efficacy of the nanosystem were further evaluated in the ulcerative colitis (UC) mice. Results: The obtained AHP/CS/OX-CD-DM nanosystem (ACOC-DM) could maintain stability under the GI pH environments, and release drug in the inflammatory colon with pH/ROS sensitivity. Dual polysaccharide-coated ACOC-DM exhibited higher cellular uptake and anti-inflammatory efficacy in macrophages than single polysaccharide-coated CS/OX-CD-DM nanosystem (COC-DM). Orally administrated ACOC-DM could enhance inflammation targeting ability and therapeutic efficacy of DM in the UC mice. Conclusion: This carbohydrate-based nanosystem with pH/ROS dual sensitivity and inflammation targeting capacity may serve as a safe and versatile nanoplatform for IBD therapy.
Subject(s)
Anti-Inflammatory Agents , Chitosan , Colitis, Ulcerative , Dexamethasone , Pectins , Animals , Colitis, Ulcerative/drug therapy , Mice , Chitosan/chemistry , Dexamethasone/chemistry , Dexamethasone/administration & dosage , Dexamethasone/pharmacokinetics , Dexamethasone/pharmacology , Pectins/chemistry , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/administration & dosage , Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/pharmacokinetics , Cyclodextrins/chemistry , Cyclodextrins/pharmacology , Drug Delivery Systems/methods , Drug Liberation , RAW 264.7 Cells , Hydrogen-Ion Concentration , Humans , Reactive Oxygen Species/metabolism , Colon/drug effects , Colon/metabolism , Drug Carriers/chemistry , Male , Nanoparticles/chemistryABSTRACT
The honeybee gut microbiome is crucial for degrading diverse pollen glycans. Yet it is unclear how this process shapes the interactions among bacteria. Here, we demonstrate a conditional mutualistic interaction between strains of two honeybee gut bacteria Bifidobacterium asteroides and Gilliamella apicola. When co-occurring in vitro and in vivo, Bifidobacterium provides complementary demethylation service to promote Gilliamella growth on methylated homogalacturonan, an enriched polysaccharide of pectin. In exchange, Gilliamella shares digestive products with Bifidobacterium, through which a positive interaction is established. This positive interaction vanishes when Bifidobacterium is not required on a non-methylated diet. Results from biochemical and gene expression analyses combined with model simulation further suggest that the ratio change of the two major homogalacturonan breakdown products, galacturonic acid (GalA) and di-GalA, determines the bacterial interaction. This study unravels how glycan metabolism may shape the interactions between honeybee gut bacteria.
Subject(s)
Bifidobacterium , Gastrointestinal Microbiome , Pectins , Symbiosis , Bees/microbiology , Animals , Pectins/metabolism , Gastrointestinal Microbiome/physiology , Symbiosis/physiology , Bifidobacterium/metabolism , Bifidobacterium/genetics , Polysaccharides/metabolism , Hexuronic AcidsABSTRACT
This study investigated the effectiveness of a pectin-nano zero-valent iron-based nanocomposite in adsorbing heavy metals in bimetallic form (chromiumlead mixture), along with assessing its antibacterial properties. The nanocomposite was synthesized using a straightforward dispersion method, employing eco-friendly components like biocompatible pectin sourced from banana peels and nano-scale zero-valent iron. Analytical characterization confirmed the formation of stable, nano-crystalline particles with active interactions between the functional groups of pectin and nano iron. Batch adsorption experiments optimized various parameters such as pH, adsorbent dosage, contact time, metal ion concentration, and temperature to enhance bimetal removal from water. The optimal conditions were determined as pH 8.0, a temperature of 40 °C, 1.0 g/L adsorbent dosage, 75 mg/l initial bimetal concentration, and a contact time of 30 min. Further assessments revealed that the nanocomposite did not induce phytotoxic or ecotoxic effects, confirming its non-toxicity and environmental safety. Biocompatibility studies conducted using zebrafish models showed no adverse effects on hatching, survival, or heart rate. These findings underscore the potential of the nanocomposite as a sustainable and efficient solution for heavy metal remediation in water treatment process.
Subject(s)
Anti-Bacterial Agents , Iron , Metals, Heavy , Nanocomposites , Pectins , Water Pollutants, Chemical , Water Purification , Zebrafish , Nanocomposites/chemistry , Pectins/chemistry , Pectins/pharmacology , Iron/chemistry , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Metals, Heavy/chemistry , Water Pollutants, Chemical/chemistry , Water Purification/methods , Adsorption , Animals , Hydrogen-Ion Concentration , Green Chemistry TechnologyABSTRACT
Coated fertilizers have been widely used to improve fertility in barren land. However, improving soil structure and water-retention capacity is also essential for arid and semi-arid areas with sandy soils to promote crop growth. Most currently available coated fertilizers rarely meet these requirements, limiting their application scope. Therefore, this study "tailored" pectin-montmorillonite (PM) multifunctional coatings for arid areas, featuring intercalation reactions and nanoscale entanglement between pectin and montmorillonite via hydrogen bonding and electrostatic and van der Waals forces. Notably, PM coatings have demonstrated an effective "relay" model of action. First, the PM-50 coating could act as a "shield" to protect urea pills, increasing the mechanical strength (82.12 %). Second, this coating prolonged the release longevity of urea (<0.5 h to 15 days). Further, the remaining coating performed a water-retention function. Subsequently, the degraded coating improved the soil properties. Thus, this coating facilitated the growth of wheat seedlings in a simulated arid environment. Moreover, the cytotoxicity test, life cycle assessment, and soil biodegradation experiment showed that the PM coating exhibited minimal environmental impact. Overall, the "relay" model of PM coating overcomes the application limitations of traditional coated fertilizers and provides a sustainable strategy for developing coating materials in soil degradation areas.
Subject(s)
Bentonite , Delayed-Action Preparations , Fertilizers , Pectins , Soil , Water , Pectins/chemistry , Water/chemistry , Soil/chemistry , Bentonite/chemistry , Delayed-Action Preparations/chemistry , Biodegradation, Environmental , Triticum/chemistry , Urea/chemistryABSTRACT
N-doped TiO2/carbon composites (N-TiPC) have shown excellent photodegradation performances to the organic contaminants but are limited by the multistage preparation (i.e., preparation of porous carbon, preparation of N-doped TiO2, and loading of N-doped TiO2 on porous carbon). Here, we develop a handy way by combining the Pickering emulsion-gel template route and chelation reaction of polysaccharides. The N-TiPC is obtained by calcinating pectin/Dl-serine hydrazide hydrochloride (SHH)-Ti4+ chelate and is further described by modern characterization techniques. The results show that the N atom is successfully doped into the TiO2 lattice, and the bandgap value of N-TiPC is reduced to 2.3 eV. Moreover, the particle size of N-TiPC remains about 10 nm. The configurations of the composites are simulated using DFT calculation. The photocatalytic experiments show that N-TiPC has a high removal efficiency for methylene blue (MB) and oxytetracycline hydrochloride (OTC-HCL). The removal ratios of MB (20 mg/L, 50 mL) and OTC-HCL (30 mg/L, 50 mL) are 99.41 % and 78.29 %, respectively. The cyclic experiments show that the photocatalyst has good stability. Overall, this study provides a handy way to form N-TiPC with enhanced photodegradation performances. It can also be promoted to other macromolecules such as cellulose and its derivatives, sodium alginate, chitosan, lignin, etc.
Subject(s)
Carbon , Pectins , Serine , Titanium , Pectins/chemistry , Titanium/chemistry , Carbon/chemistry , Serine/chemistry , Nitrogen/chemistry , Catalysis , Photolysis , Porosity , Methylene Blue/chemistryABSTRACT
BACKGROUND: Key wound environment parameters include pH, hydration, and the balance between tissue remodeling and deposition of new tissue. When prolonged inflammation is present, the proliferation phase of wound healing can be delayed because excessive protease production due to persistent inflammation can destroy newly formed tissue and prevent wounds from filling and reepithelializing. OBJECTIVE: To conduct an in vitro study of the ability of polygalacturonic acid (PG), a natural pectin derivative present in ripening fruit, to inhibit 3 destructive wound proteases and prevent dehydration in environments in which significant evaporation can occur. MATERIALS AND METHODS: In vitro enzyme inhibition assay kits were used to detect the ability of PG to inhibit key wound proteases matrix metalloproteinase (MMP)-2, MMP-9, and neutrophil elastase (NE). Transepidermal evaporative water loss from a polyvinyl alcohol skin substitute hydrogel was gravimetrically measured. RESULTS: PG could partially inhibit MMP-2 (>50% inhibition relative to negative controls), MMP-9 (>50% inhibition relative to negative controls), and NE (>25% inhibition relative to negative controls) and thereby potentially blunt some of the destructive effects of excess proteases where prolonged inflammation is present. In an in vitro transepidermal evaporative water loss assay, PG also helped retain moisture and inhibited dehydration (>25% reduction relative to negative controls). CONCLUSIONS: These findings suggest that PG can be a useful addition to ointments and dressings in wound care and warrants further in vivo testing.
Subject(s)
Dehydration , Matrix Metalloproteinase 2 , Matrix Metalloproteinase 9 , Pectins , Wound Healing , Wound Healing/drug effects , Pectins/pharmacology , Humans , Matrix Metalloproteinase 9/metabolism , Matrix Metalloproteinase 2/metabolism , Matrix Metalloproteinase Inhibitors/pharmacology , Leukocyte Elastase/metabolism , Leukocyte Elastase/antagonists & inhibitors , Wounds and Injuries/pathology , Water Loss, Insensible/drug effectsABSTRACT
Interaction of microbiota with hybrid vaterite-pectin microparticles as an attractive multifunctional vehicle for mucosal delivery should not provoke inflammation. Our purpose was to study the reaction of bacteria E. coli strain Mg1655 and isolate SharL from a patient with Crohn disease on the cultivation with hybrid microparticles and vaterite, and the subsequent activation of neutrophils. Vaterite-pectin microparticles enhanced leakage of ATP from bacteria. For E. coli Mg1655, the concentration of DNA decreased, while intracellular ATP increased. For E. coli SharL, the intracellular ATP decreased with simultaneous growth of DNA. Bacteria and microparticles together did not enhance activation of neutrophils in comparison with the particles per se in the medium without serum and in comparison with bacteria in the medium supplemented with serum; microparticles did not reduce functional activity of neutrophils.
Subject(s)
Escherichia coli , Neutrophils , Pectins , Humans , Neutrophils/drug effects , Neutrophils/metabolism , Escherichia coli/drug effects , Pectins/pharmacology , Adenosine Triphosphate/metabolism , Calcium Carbonate/pharmacology , Calcium Carbonate/chemistry , Crohn Disease/microbiology , Crohn Disease/pathology , Neutrophil Activation/drug effectsABSTRACT
A fiber-rich diet is considered beneficial for gut health. An inflamed gut with a dysbiotic bacterial community can result in altered fiber metabolism depending on the fiber's physicochemical properties. This study examined the effect of fiber's physicochemical properties on fiber fermentation in the presence of healthy and colitis-associated bacteria. Sixteen fibers with different levels of solubility, complexity, and fermentation rate were used in in vitro fermentation with healthy human gut bacteria. Resistant maltodextrins (RMD), pectin (HMP), inulin (ChIn), and wheat bran (WB) were selected for fermentation using ulcerative colitis (UC)-associated bacteria to assess bacterial dysbiosis effect. UC-associated gut microbiota showed a significant reduction in α-and ß-diversity indices compared to healthy-associated microbiota. The differences in the gut microbiota composition and diversity between the donors resulted in decreased fermentation rates with UC-associated bacteria. Fiber fermentation metabolites, short-chain fatty acids (SCFA) and gas production were significantly lower in the presence of UC-associated bacteria for all four fibers tested. Overall, we conclude that dietary fiber properties and microbial dysbiosis are influential in fiber fermentation and metabolite production in the gut.
Subject(s)
Bacteria , Dietary Fiber , Dysbiosis , Fatty Acids, Volatile , Fermentation , Gastrointestinal Microbiome , Dietary Fiber/pharmacology , Gastrointestinal Microbiome/physiology , Humans , Dysbiosis/microbiology , Bacteria/metabolism , Bacteria/classification , Fatty Acids, Volatile/metabolism , Pectins/metabolism , Colitis, Ulcerative/microbiology , Inulin/metabolism , Male , Adult , Female , PolysaccharidesABSTRACT
The release of the model drug theophylline from silica-pectin aerogels was investigated. The composite aerogels were prepared via impregnation of pectin alcogels with silica sol, followed by in situ silica gelation and drying with supercritical CO2. The structural and physico-chemical properties of the aerogels were tuned via the preparation conditions (type of silica sol, calcium crosslinking of pectin or not). Theophylline was loaded via impregnation and its release into simulated gastric fluid was studied during 1 h followed by release into simulated intestinal fluid. The swelling, mass loss and theophylline release behavior of the composites were analyzed and correlated with material properties. It followed that only aerogels prepared with calcium-crosslinked pectin and polyethoxydisiloxane were stable in aqueous systems, exhibiting a slow theophylline release governed by near-Fickian diffusion.
Subject(s)
Drug Delivery Systems , Gels , Pectins , Silicon Dioxide , Theophylline , Pectins/chemistry , Silicon Dioxide/chemistry , Theophylline/chemistry , Theophylline/administration & dosage , Gels/chemistry , Drug Delivery Systems/methods , Drug Liberation , Drug Carriers/chemistryABSTRACT
This study aimed to isolate Ulva pertusa polysaccharide (UPP), which elicits anti-inflammatory bowel disease (IBD) effects, from the Korea seaweed U. pertusa and identify its structure. Firstly, UPP was isolated from U. pertusa using hydrothermal extraction and ethanol precipitation. UPP is a novel polysaccharide that exhibits unique structural features such as 3-sulfated rhamnose, glucuronic acid, iduronic acid, and 3-sulfated xylose, which are repeated in 1,4-glycosidic bonds. Prophylactic oral administration of UPP in mice with dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) suppressed the levels of inflammatory cytokines and MAPK- and NF-κB-related factors in the serum and colon tissue. Tight junction (TJ)-related factors such as occludin, claudin-1, and mucin were effectively augmented by UPP in the colon tissue. In addition, UPP administration prevented the DSS treatment-led cecal short chain fatty acid imbalance, and this effect was most evident for propionic acid. In conclusion, UPP isolated from the Korean U. pertusa demonstrates potent anti-IBD activity. Characterization of this ulvan revealed its unique structure. Moreover, its efficacy may be associated with its anti-inflammatory effects and regulation of gut microbiota and TJ proteins. Thus, this study provides new insights into the biological effects of UPP in IBD.
Subject(s)
Ulva , Animals , Ulva/chemistry , Mice , Dextran Sulfate , Colitis, Ulcerative/drug therapy , Colitis, Ulcerative/chemically induced , Male , Pectins/chemistry , Pectins/pharmacology , Pectins/isolation & purification , Fatty Acids, Volatile/metabolism , Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/isolation & purification , Anti-Inflammatory Agents/therapeutic use , Colon/drug effects , Colon/pathology , Seaweed/chemistry , Inflammatory Bowel Diseases/drug therapy , Cytokines/metabolismABSTRACT
This work demonstrates that sesame (Sesamum indicum L.) hull, an unexploited food industrial waste, can be used as an efficient source for the extraction of hemicellulose and/or pectin polysaccharides to further obtain functional oligosaccharides. Different polysaccharides extraction methods were surveyed including alkaline and several enzymatic treatments. Based on the enzymatic release of xylose, arabinose, glucose, and galacturonic acid from sesame hull by using different enzymes, Celluclast®1.5 L, Pectinex®Ultra SP-L, and a combination of them were selected for the enzymatic extraction of polysaccharides at 50 °C, pH 5 up to 24 h. Once the polysaccharides were extracted, Ultraflo®L was selected to produce arabinoxylo-oligosaccharides (AXOS) at 40 °C up to 24 h. Apart from oligosaccharides production from extracted polysaccharides, alternative approaches for obtaining oligosaccharides were also explored. These were based on the analysis of the supernatants resulting from the polysaccharide extraction, alongside a sequential hydrolysis performed with Celluclast®1.5 L and Ultraflo®L of the starting raw sesame hull. The different fractions obtained were comprehensively characterized by determining low molecular weight carbohydrates and monomeric compositions, average Mw and dispersity, and oligosaccharide structure by MALDI-TOF-MS. The results indicated that sesame hull can be a useful source for polysaccharides extraction (pectin and hemicellulose) and derived oligosaccharides, especially AXOS.
Subject(s)
Oligosaccharides , Sesamum , Sesamum/chemistry , Oligosaccharides/chemistry , Hydrolysis , Polysaccharides/chemistry , Xylans/chemistry , Xylans/isolation & purification , Pectins/chemistry , Pectins/isolation & purification , Industrial Waste , Arabinose/chemistry , Xylose/chemistryABSTRACT
Biopolymers such as chitosan and pectin are currently attracting significant attention because of their unique properties, which are valuable in the food industry and pharmaceutical applications. These properties include non-toxicity, compatibility with biological systems, natural decomposition ability, and structural adaptability. The objective of this study was to assess the performance of two different ratios of pectin-chitosan polyelectrolyte composite (PCPC) after applying them as a coating to commercially pure titanium (CpTi) substrates using electrospraying. The PCPC was studied in ratios of 1:2 and 1:3, while the control group consisted of CpTi substrates without any coating. The pull-off adhesion strength, cytotoxicity, and antibacterial susceptibility tests were utilized to evaluate the PCPC coatings. In order to determine whether the composite coating was the result of physical blending or chemical bonding, the topographic surface parameters were studied using Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). PCPC (1:3) had the highest average cell viability of 93.42, 89.88, and 86.85% after 24, 48, and 72 h, respectively, as determined by the cytotoxicity assay, when compared to the other groups. According to the Kirby-Bauer disk diffusion method for testing antibacterial susceptibility, PCPC (1:3) showed the highest average diameter of the zone of inhibition, measuring 14.88, 14.43, and 11.03 mm after 24, 48, and 72 h of incubation, respectively. This difference was highly significant compared to Group 3 at all three time periods. PCPC (1:3) exhibited a significantly higher mean pull-off adhesion strength (521.6 psi) compared to PCPC (1:2), which revealed 419.5 psi. PCPC (1:3) coated substrates exhibited better surface roughness parameters compared to other groups based on the findings of the AFM. The FTIR measurement indicated that both PCPC groups exhibited a purely physical blending in the composite coating. Based on the extent of these successful in vitro experiments, PCPC (1:3) demonstrates its potential as an effective coating layer. Therefore, the findings of this study pave the way for using newly developed PCPC after electrospraying coating on CpTi for dental implants.
Subject(s)
Anti-Bacterial Agents , Chitosan , Dental Implants , Pectins , Polyelectrolytes , Chitosan/chemistry , Chitosan/pharmacology , Pectins/chemistry , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Polyelectrolytes/chemistry , Microbial Sensitivity Tests , Coated Materials, Biocompatible/chemistry , Coated Materials, Biocompatible/pharmacology , Spectroscopy, Fourier Transform Infrared , Animals , Titanium/chemistry , Titanium/pharmacology , Materials Testing , Cell Survival/drug effects , Humans , Microscopy, Atomic Force , Surface Properties , MiceABSTRACT
Zinc (Zn) is an essential micronutrient but can be cytotoxic when present in excess. Plants have evolved mechanisms to tolerate Zn toxicity. To identify genetic loci responsible for natural variation of plant tolerance to Zn toxicity, we conduct genome-wide association studies for root growth responses to high Zn and identify 21 significant associated loci. Among these loci, we identify Trichome Birefringence (TBR) allelic variation determining root growth variation in high Zn conditions. Natural alleles of TBR determine TBR transcript and protein levels which affect pectin methylesterification in root cell walls. Together with previously published data showing that pectin methylesterification increase goes along with decreased Zn binding to cell walls in TBR mutants, our findings lead to a model in which TBR allelic variation enables Zn tolerance through modulating root cell wall pectin methylesterification. The role of TBR in Zn tolerance is conserved across dicot and monocot plant species.
Subject(s)
Arabidopsis , Cell Wall , Gene Expression Regulation, Plant , Pectins , Plant Roots , Zinc , Cell Wall/metabolism , Cell Wall/drug effects , Cell Wall/genetics , Plant Roots/metabolism , Plant Roots/drug effects , Plant Roots/growth & development , Plant Roots/genetics , Zinc/metabolism , Zinc/toxicity , Pectins/metabolism , Arabidopsis/genetics , Arabidopsis/metabolism , Arabidopsis/drug effects , Arabidopsis/growth & development , Gene Expression Regulation, Plant/drug effects , Esterification , Arabidopsis Proteins/metabolism , Arabidopsis Proteins/genetics , Genome-Wide Association Study , Alleles , Genetic VariationABSTRACT
The use of essential oils is widespread in various fields such as pharmacy, pest control, and active packaging. However, their instability and short-term effects require methods to enhance their durability and effectiveness. Encapsulation in biopolymer matrices appears to be a promising approach due to the environmental safety and cost-effectiveness of such formulations. In this study, different oil-in-water emulsions were prepared by mixing chitosan-gelatin (C-G) or pectin-gelatin (P-G) solutions with lemongrass essential oil (LG). ZnO NPs were used as an additional active component. Encapsulation in biopolymer matrices resulted in stable emulsions with a significantly slower release of LG, and ZnO NPs further suppressed LG release, particularly in the P-G emulsion. They also contributed to the stability of the emulsions and a decrease in the average droplet size of LG. Furthermore, the presence of LG and ZnO NPs improved the smoothness of the films prepared from the emulsions and dispersions using the casting technique. SEM/EDS analysis confirmed the homogeneous distribution of ZnO NPs in both C-G and P-G films. By adjusting the type and content of the biopolymers and NPs, such emulsions could be effectively utilized in various applications where controlled release of active components is required.
Subject(s)
Chitosan , Emulsions , Gelatin , Oils, Volatile , Pectins , Zinc Oxide , Gelatin/chemistry , Zinc Oxide/chemistry , Chitosan/chemistry , Pectins/chemistry , Oils, Volatile/chemistry , Emulsions/chemistry , Biopolymers/chemistry , Nanoparticles/chemistry , Sterculia/chemistryABSTRACT
Environmental pollution remains a constant challenge due to the indiscriminate use of fossil fuels, mining activities, chemicals, drugs, aromatic compounds, pesticides, etc. Many emerging pollutants with no fixed standards for monitoring and control are being reported. These have adverse impacts on human life and the environment around us. This alarms the wastewater management towards developing materials that can be used for bulk water treatment and are easily available, low cost, non-toxic and biodegradable. Waste biomass like pectin is extracted from fruit peels which are a discarded material. It is used in pharmaceutical and nutraceutical applications but its application as a material for water treatment is very limited in literature. The scientific gap in literature review reports are evident with discussion only on pectin based hydrogels or specific pectin derivatives for some applications. This review focuses on the chemistry, extraction, functionalization and production of pectin derivatives and their applications in water treatment processes. Pectin functionalized derivatives can be used as a flocculant, adsorbent, nano biopolymer, biochar, hybrid material, metal-organic frameworks, and scaffold for the removal of heavy metals, ions, toxic dyes, and other contaminants. The huge quantum of pectin biomass may be explored further to strengthen environmental sustainability and circular economy practices.
Subject(s)
Biomass , Pectins , Water Purification , Pectins/chemistry , Water Purification/methods , Water Pollutants, Chemical/chemistry , Wastewater/chemistry , AdsorptionABSTRACT
In this study, the effects of different ultrasonic treatment intensities (57, 170, and 283 W/cm2) on the chemical composition, molecular chain characteristics, crystal structure, micromorphology, interfacial adsorption behavior and emulsifying properties of sugar beet pectin (SBP) were investigated. Ultrasonic treatment did not change the types of SBP monosaccharides, but it had impacts on their various monosaccharide contents. Moreover, the feruloylated, acetyl, and methoxy groups of SBP also undergo varying degrees of changes. The increase in ultrasonic treatment intensity led to transition in the molecular chain conformation of SBP from rigid semi-flexible chains to flexible chains, accompanied by modification in its crystal structure. Microstructural analysis of SBP confirmed the significant change in molecular chain conformation. Modified SBP could form an elastic interfacial film with higher deformation resistance on the oil-water interface. The SBP sample modified with 170 W/cm2 exhibited better emulsifying properties owing to its better interfacial adsorption behavior. Moreover, the emulsions prepared with modified SBP exhibited better stability capability under different environmental stresses (pH value, salt ion concentration, heating temperature and freeze-thaw treatment). The results revealed that the ultrasonic technology is useful to improve the emulsifying properties of SBP.
Subject(s)
Beta vulgaris , Pectins , Beta vulgaris/chemistry , Pectins/chemistry , Emulsions/chemistry , Ultrasonic Waves , Adsorption , Molecular Conformation , Hydrogen-Ion ConcentrationABSTRACT
There has been a long-standing bottleneck in the quantitative analysis of the frequencies of homoblock polyads beyond triads using 1H and 13C NMR for linear polysaccharides, primarily because monosaccharides within a long homoblock share similar chemical environments due to identical neighboring units, resulting in indistinct NMR peaks. In this study, through rigorous mathematical induction, inequality relations were established that enabled the calculation of frequency ranges of homoblock polyads from historically reported NMR-derived frequency values of diads and/or triads of alginates, chitosans, homogalacturonans, and galactomannans. The calculated homoblock frequency ranges were then applied to evaluate three chain growth statistical models, including the Bernoulli chain, first-order Markov chain, and second-order Markov chain, for predicting homoblock frequencies in these polysaccharides. Furthermore, based on the mathematically derived inequality relations, a novel 2D array was constructed, enabling the graphical visualization of homoblock features in polysaccharides. It was demonstrated, as a proof of concept, that the novel 2D array, along with a 1D code generated from it, could serve as an effective feature engineering tool for polymer classification using machine learning algorithms.