ABSTRACT
We show that filamentous fungi can emit green or red light after the accumulation of particulate lanthanide metal-organic frameworks over the cell wall. These new biohybrids present photoluminescence properties that are unaffected by the components of the cell wall. In addition, the fungal cells internalise lanthanide metal-organic framework particles, storing them into organelles, thereby making these materials promising for applications in living imaging studies.
Subject(s)
Fungi/metabolism , Intravital Microscopy/methods , Lanthanoid Series Elements/chemistry , Luminescence , Metal-Organic Frameworks/chemistry , Color , Cytoplasm/chemistry , Cytoplasm/metabolism , Cytoplasm/radiation effects , Feasibility Studies , Fungi/chemistry , Fungi/cytology , Fungi/radiation effects , Lanthanoid Series Elements/metabolism , Lanthanoid Series Elements/radiation effects , Luminescent Measurements/methods , Metal-Organic Frameworks/metabolism , Metal-Organic Frameworks/radiation effects , Microbiological Techniques/methods , Organelles/chemistry , Organelles/metabolism , Organelles/radiation effects , Ultraviolet RaysABSTRACT
A common side effect of radiotherapy used in the treatment of oral cancer is the occurrence of structural and physiological alterations of the salivary glands due to exposure to ionizing radiation, as demonstrated by conditions such as decreased salivary flow. The present study evaluated ultrastructural alterations in the parotid glands of rats receiving a fractionated dose (1,500-cGy) of radiation emitted by a Cesium-137 source and rats that were not subjected to ionizing radiation. After sacrifice, the parotid glands were removed and examined by transmission electron microscopy. Damage such as cytoplasmic vacuolization, dilatation of the endoplasmic reticulum and destruction of mitochondria, as well as damage to the cellular membrane of acinar cells, were observed. These findings lead to the conclusion that ionizing radiation promotes alterations in the glandular parenchyma, and that these alterations are directly related to the dose level of absorbed radiation. Certain phenomena that appear in the cytoplasm and nuclear material indicate that ionizing radiation causes acinar cell death (apoptosis).
Subject(s)
Parotid Gland/radiation effects , Animals , Apoptosis , Cell Death/radiation effects , Cell Membrane/radiation effects , Cell Nucleus/radiation effects , Cesium Radioisotopes , Chromatin/radiation effects , Cytoplasm/radiation effects , Desmosomes/radiation effects , Dose Fractionation, Radiation , Endoplasmic Reticulum/radiation effects , Male , Microscopy, Electron, Transmission , Mitochondria/radiation effects , Mitochondrial Membranes/radiation effects , Parotid Gland/ultrastructure , Rats , Rats, Wistar , Vacuoles/radiation effectsABSTRACT
A common side effect of radiotherapy used in the treatment of oral cancer is the occurrence of structural and physiological alterations of the salivary glands due to exposure to ionizing radiation, as demonstrated by conditions such as decreased salivary flow. The present study evaluated ultrastructural alterations in the parotid glands of rats receiving a fractionated dose (1,500-cGy) of radiation emitted by a Cesium-137 source and rats that were not subjected to ionizing radiation. After sacrifice, the parotid glands were removed and examined by transmission electron microscopy. Damage such as cytoplasmic vacuolization, dilatation of the endoplasmic reticulum and destruction of mitochondria, as well as damage to the cellular membrane of acinar cells, were observed. These findings lead to the conclusion that ionizing radiation promotes alterations in the glandular parenchyma, and that these alterations are directly related to the dose level of absorbed radiation. Certain phenomena that appear in the cytoplasm and nuclear material indicate that ionizing radiation causes acinar cell death (apoptosis).
Um efeito colateral comum da radioterapia usada no tratamento de câncer na cavidade oral é a ocorrência de alterações estruturais e fisiológicas sobre as glândulas salivares por exposição à radiação ionizante, como demonstrada em situações com decréscimo do fluxo salivar. O presente estudo teve por objetivo avaliar as alterações ultra-estruturais de glândulas parótidas de ratos que receberam uma dose fracionada (1500 - cGy) de radiação emitida por uma fonte de Césio 137 e ratos que não receberam a radiação ionizante. Após o sacrifício, as glândulas parótidas foram removidas e examinadas por microscopia eletrônica de transmissão. Lesões das organelas citoplasmáticas, como dilatação do retículo endoplasmático, destruição das mitocôndrias e formação das vacuolizações citoplasmáticas, além de lesão da membrana celular das células acinares foram observadas. Portanto, a radiação ionizante promove alterações no parênquima glandular, o que está diretamente relacionado com a dose de radiação absorvida. Determinados fenômenos que surgem no citoplasma e material nuclear são indicadores de que a radiação ionizante leva a célula acinar a morte programada (apoptose).
Subject(s)
Animals , Male , Rats , Parotid Gland/radiation effects , Apoptosis , Cesium Radioisotopes , Cell Death/radiation effects , Cell Membrane/radiation effects , Cell Nucleus/radiation effects , Chromatin/radiation effects , Cytoplasm/radiation effects , Dose Fractionation, Radiation , Desmosomes/radiation effects , Endoplasmic Reticulum/radiation effects , Microscopy, Electron, Transmission , Mitochondria/radiation effects , Mitochondrial Membranes/radiation effects , Parotid Gland/ultrastructure , Rats, Wistar , Vacuoles/radiation effectsABSTRACT
The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/cm2 (Power=26 mW; lambda=.670 nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4, photodynamic treatment. The Aluminum phthalocyanine chloride (AlPc) photodynamic treatment induced necrosis on the neoplastic cell line, and cytoplasm and nuclear alterations on the normal cell line. (AU)
Subject(s)
Humans , Female , Cricetinae , RESEARCH SUPPORT, NON-U.S. GOVT , Uterine Cervical Neoplasms/drug therapy , Lasers , Ovary/drug effects , Photochemotherapy/methods , Radiation-Sensitizing Agents/pharmacology , Apoptosis/drug effects , Apoptosis/physiology , Apoptosis/radiation effects , CHO Cells , Cell Nucleus/drug effects , Cell Nucleus/radiation effects , Cell Nucleus/ultrastructure , Uterine Cervical Neoplasms/ultrastructure , Cytoplasm/drug effects , Cytoplasm/radiation effects , Cytoplasm/ultrastructure , HeLa Cells , Indoles/pharmacology , Light , Microscopy, Electron , Mitochondria/drug effects , Mitochondria/radiation effects , Mitochondria/ultrastructure , Necrosis , Organometallic Compounds/pharmacology , Ovary/ultrastructure , Photic Stimulation/instrumentation , Photic Stimulation/methodsABSTRACT
The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/cm2 (Power=26 mW; lambda=.670 nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4, photodynamic treatment. The Aluminum phthalocyanine chloride (AlPc) photodynamic treatment induced necrosis on the neoplastic cell line, and cytoplasm and nuclear alterations on the normal cell line.
Subject(s)
Humans , Female , Cricetinae , Photochemotherapy/methods , Lasers , Uterine Cervical Neoplasms/drug therapy , Ovary/drug effects , Radiation-Sensitizing Agents/pharmacology , Apoptosis/drug effects , Apoptosis/physiology , Apoptosis/radiation effects , CHO Cells , Cytoplasm/drug effects , Cytoplasm/radiation effects , Cytoplasm/ultrastructure , Organometallic Compounds/pharmacology , Photic Stimulation/instrumentation , Photic Stimulation/methods , HeLa Cells , Indoles/pharmacology , Light , Microscopy, Electron , Mitochondria/drug effects , Mitochondria/radiation effects , Mitochondria/ultrastructure , Necrosis , Uterine Cervical Neoplasms/ultrastructure , Cell Nucleus/drug effects , Cell Nucleus/radiation effects , Cell Nucleus/ultrastructure , Ovary/ultrastructureABSTRACT
The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/cm2 (Power=26 mW; lambda=.670 nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4, photodynamic treatment. The Aluminum phthalocyanine chloride (AlPc) photodynamic treatment induced necrosis on the neoplastic cell line, and cytoplasm and nuclear alterations on the normal cell line.