RÉSUMÉ
Four piroxicam metal complexes; NiL2 , PtL2 , PdL2 , and AgL were synthesized and characterized by different techniques with enhanced antibacterial and anticancer activity. Regarding in vitro antimicrobial activity, complex NiL2 displayed potent antibacterial effect against Escherichia coli and Pseudomonas aeruginosa that was 1.9-folds higher than piroxicam (minimum inhibitory concentration [MIC] = 31.85, 65.32 µM), respectively. In case of G+ve bacteria, complex PtL2 had potent activity on Staphylococcus aureus which was 2.1-folds higher than piroxicam (MIC = 43.12 µM), while activity of complex AgL against Enterococcus faecalis was threefolds higher than piroxicam (MIC = 74.57 µM. Complexes PtL2 and PdL2 exhibited higher inhibition of DNA gyrase than piroxicam (IC50 = 6.21 µM) in the range of 1.9-1.7-folds. The in vitro antiproliferative activity depicted that all investigated complexes showed better cytotoxic effect than piroxicam, specifically Pt and Pd complexes which had lower IC50 values than piroxicam on human liver cancer cell line HepG2 by 1.8 and 1.7-folds, respectively. While Pd and Ag complexes showed 2 and 1.6-folds better effect on human colon cancer cell line HT-29 compared with piroxicam. Molecular modeling studies including docking on Stranded DNA Duplex (1juu) and DNA gyrase enzyme (1kzn) that gave good insight about interaction of complexes with target molecules, calculation of electrostatic potential map and global reactivity descriptors were performed.
Sujet(s)
Antinéoplasiques , Complexes de coordination , Humains , Piroxicam/pharmacologie , Complexes de coordination/pharmacologie , DNA gyrase , Antibactériens/pharmacologie , Antinéoplasiques/pharmacologie , Tests de sensibilité microbienne , Simulation de docking moléculaireRÉSUMÉ
Quinoa is one of the highest nutritious grains, and global consumption of quinoa flour has increased as people pay more attention to health. Due to its high value, quinoa flour is susceptible to adulteration. Cross-contamination between quinoa flour and other flour can be easily neglected due to their highly similar appearance. Therefore, detecting adulteration in quinoa flour is important to consumers, industries, and regulatory agencies. In this study, portable hyperspectral imaging in the visible near-infrared (VNIR) spectral range (400-1000 nm) was applied as a rapid tool to detect adulteration in quinoa flour. Quinoa flour was adulterated with wheat, rice, soybean, and corn in the range of 0-98% with 2% increments. Partial least squares regression (PLSR) models were developed, and the best model for detecting the % authentic flour (quinoa) was obtained by the raw spectral data with R2p of 0.99, RMSEP of 3.08%, RPD of 8.77, and RER of 25.32. The model was improved, by selecting only 13 wavelengths using bootstrapping soft shrinkage (BOSS), to R2p of 0.99, RMSEP of 2.93%, RPD of 9.18, and RER of 26.60. A visualization map was also generated to predict the level of quinoa in the adulterated samples. The results of this study demonstrate the ability of VNIR hyperspectral imaging for adulteration detection in quinoa flour as an alternative to the complicated traditional method.
RÉSUMÉ
BACKGROUND AND OBJECTIVE: There are several forms of relevant epi-aortic branching anomalies, and perhaps that is why different views as to the best approach have been reported. To help resolve this dilemma, we examined the unforeseen arch anomalies found at thoracoscopic repair of esophageal atresia and the outcomes. METHODS: In a retrospective cohort, all consecutive patients who were thoracoscopically approached for esophageal atresia over a 5-year period with unforeseen aortic/epi-aortic branching were identified and grouped. Thoracoscopic views, operative interventions, and outcomes were studied. RESULTS: A total of 121 neonates were thoracoscopically approached for EA, of whom 18 cases with aberrant aortic architecture were selected. Four (3%) cases were diagnosed on a preoperative echocardiography as a right-sided aortic arch, whereas unforeseen anomalous anatomies were reported in 14 cases (11.6%): left aortic arch with an aberrant right subclavian artery (ARSA) (n = 10), right-sided aortic arch with an aberrant left subclavian artery (ALSA) (n = 3), and mirror-image right arch (n = 1). Single postoperative mortality was reported among the group with left arch and ARSA (10%), whereas all the cases with right arch and ALSA died. CONCLUSIONS: In all, 11.6% of the studied series exhibited unexpected aberrant aortic architecture, with higher complication rates in comparison to the typical thoracoscopic repairs. For EA with left aortic arch and ARSA, the primary esophageal surgery could safely be completed. Meanwhile, curtailing surgery-after ligating the TEF-to get advanced imaging is still advised for both groups with the right arch due to the significant existence of vascular rings.
Sujet(s)
Malformations cardiovasculaires , Atrésie de l'oesophage , Aorte thoracique/malformations , Aorte thoracique/imagerie diagnostique , Aorte thoracique/chirurgie , Malformations cardiovasculaires/complications , Atrésie de l'oesophage/complications , Atrésie de l'oesophage/imagerie diagnostique , Atrésie de l'oesophage/chirurgie , Humains , Nouveau-né , Études rétrospectives , Artère subclavière/malformationsRÉSUMÉ
Background and Aims: Before the significance of urethral length was highlighted in patients with cloacal malformation, total urogenital mobilization using a posterior sagittal approach was recommended for common channel (CC) length <3 cm, those >3 cm it was followed by urogenital separation. However, many urologists are advocating that the urethral length rather than length of the CC should influence the choice of operation. It is also recommended that total urogenital mobilization should be avoided in patients with short urethral length as intraoperative decision to shift to urogenital separation will risk devascularization of the urethra, advocating total urogenital separation (TUS) from the start; the later technique was deemed difficult using open approach. We describe our experience with laparoscopic TUS and rectal pull-through in patients with cloacal malformation. Methods: Six patients were operated for a period of 3 years from December 2017 to July 2021; they underwent laparoscopic TUS and rectal pull-through. Preoperative investigations included cystoscopy, genitogram, and MRI pelvis and abdominal ultrasound. IRB approval has been obtained from research ethical committee at Cairo University. Results: Six female patients born with single perineal opening had colostomy at birth. Age during the second operation ranged from 1 to 4 years. Length of the CC ranged between 2 and 5 cm. Proximal urethral length ranged between 0.5 and 1.5 cm and vaginal depth >3 cm. Average operative time was 4.25 hours. Postoperative period was 1-5 days and uneventful. On the long-term follow-up. No patient developed urethrovaginal fistula and one patient developed vaginal stenosis. All patients had no urinary problems, dry over 4-hour interval, voiding spontaneously, and had normal kidney functions. Conclusions: Laparoscopic urogenital separation, as well as vaginal and rectal pull-through for cloacal malformation, is feasible in cloacal malformation providing anatomical repair.
Sujet(s)
Laparoscopie , Vagin , Animaux , Enfant d'âge préscolaire , Cloaque/malformations , Cloaque/chirurgie , Sténose pathologique/chirurgie , Femelle , Humains , Nourrisson , Nouveau-né , Rectum/chirurgie , Vagin/chirurgieRÉSUMÉ
In this work, a comparative study was made of different magnesium ion content incorporated into hydroxyapatite (HAP) and modified with selenite ions, with the aim to develop the degradation performance of methylene blue. Although the dopant metal (Mg2+ ) was present at a relatively low ratio, it induced a change in the microstructure, morphology, surface area, external surface charge, particle size, and degradation performance. The effect of magnesium and selenium binary doping on microstructure and degradation of methylene blue was evaluated. The external surface charge measured by zeta potential clarified that the highest negativity was -11.8 mV and this was accomplished in 1.0 Mg/Se-HAP. Furthermore, the roughness average increased from 36.8 nm, reaching 59.2 nm upon the addition of Mg(II). Moreover, transmission electron microscope micrographs showed that compositions were formed as rod shapes. The process of degradation was optimized, showing effectiveness in methylene blue degradation of 62.4% after 150 min of exposure to visible light. Electrostatic attraction and H-bonding, and coordination played vital roles in the adsorption process. The recyclability of the as-prepared compositions demonstrated that the effectiveness had been reduced to ~54.2% after five times of re-use.
Sujet(s)
Bleu de méthylène , Polluants chimiques de l'eau , Adsorption , Durapatite , Ions , Magnésium , Bleu de méthylène/composition chimique , Polluants chimiques de l'eau/analyseRÉSUMÉ
Designing a nanocomposite that accumulates biocompatibility and antimicrobial behaviour is an essential requirement for biomedical applications. Hydroxyapatite (HAP), graphene oxide, and vivianite in one ternary nanocomposite with three phases and shapes led to an increase in cell viability to 97.6% ± 4 for the osteoblast cells in vitro. The obtained nanocomposites were investigated for their structural features using X-ray diffraction, while the microstructure features were analyzed using a scanning electron microscope (SEM) and a transmission electron microscope. The analysis showed a decrease in the crystal size to 13 nm, while the HAP grains reached 30 nm. The elongated shape of vivianite reached 200 nm on SEM micrographs. The monoclinic and hexagonal crystal systems of HAP and vivianite were presented in the ternary nanocomposite. The maximum roughness peak height reached 236.1 nm for the ternary nanocomposite from 203.3 nm, while the maximum height of the roughness parameter reached 440.7 nm for the di-nanocomposite of HAP/graphene oxide from 419.7 nm. The corrosion current density reached 0.004 µA/cm2 . The ferrous (Fe2+ ) and calcium (Ca2+ ) ions released were measured and confirmed. Therefore, the morphology of the nanocomposites affected bacterial activity. This was estimated as an inhibition zone and reached 14.5 ± 0.9 and 13.4 ± 1.1 mm for Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) after 24 h. The increase in viability and the antibacterial activity refer to the compatibility of the nanocomposite in different medical applications.
Sujet(s)
Graphite , Nanocomposites , Antibactériens/pharmacologie , Durapatite , Escherichia coli , Composés du fer II , Phosphates , Staphylococcus aureusRÉSUMÉ
Ternary nanocomposites, including graphene oxide (GO), hydroxyapatite (HAP), and cadmium selenite (CdSe) have been encapsulated into nanofibrous scaffolds of polylactic acid. These compositions were indexed as HAP@PLA (C1), CdSe@PLA (C2), HAP/CdSe@PLA (C3), HAP/GO@PLA (C4), and HAP/CdSe/GO@PLA (C5). Structural confirmation is executed by XRD and XPS techniques, while FESEM performs morphological characteristics. CdSe and GO dopants cause a significant increase in nanofiber diameter, HAP/GO@PLA (C4), showing thin surface fibers with fiber diameter up to 3.1 µm, followed by HAP/CdSe/GO@PLA (C4) composite that belongs to filament size up to 2.1 µm. On the other hand, the mechanical properties reveal that the dual dopant composites HAP/CdSe@PLA (C3) and HAP/GO@PLA (C4) hit the maximum tensile fracture values with 1.49 ± 0.3 and 0.99 ± 0.2 MPa. Further, the ternary C5 composite represents the lowest contact angle of 86.1 ± 3.7°. The antibacterial activity increased from 32.4 ± 9.7 and 28.4 ± 6.5% to be 85.3 ± 4.6 and 88.1 ± 5.6% for C1 and C5, respectively, against both E. coli and S. aureus in dark conditions. Moreover, the antibacterial potency enhanced from 75.4 ± 7.6 to be 83.5 ± 6.5 from dark to light conditions against E. coli for the composition of PLA containing the binary composition of HAP/CdSe.
Sujet(s)
Antibactériens/composition chimique , Composés du cadmium/composition chimique , Durapatite/composition chimique , Graphite/composition chimique , Nanocomposites , Nanofibres , Polyesters/composition chimique , Composés du sélénium/composition chimique , Structures d'échafaudage tissulaires , Cicatrisation de plaie , Antibactériens/pharmacologie , Composés du cadmium/pharmacologie , Adhérence cellulaire , Lignée cellulaire , Prolifération cellulaire , Préparation de médicament , Escherichia/effets des médicaments et des substances chimiques , Escherichia/croissance et développement , Fibroblastes/physiologie , Humains , Nanotechnologie , Composés du sélénium/pharmacologie , Staphylococcus aureus/effets des médicaments et des substances chimiques , Staphylococcus aureus/croissance et développement , Propriétés de surface , Résistance à la tractionRÉSUMÉ
Au@Se core-shell nanoparticles were obtained via laser ablation technique to be incorporated into polycaprolactone (PCL) nanofibrous scaffolds for wound healing applications at different contributions of Se nanoparticles (SeNPs). The synthesized layers were inspected via X-ray diffraction (XRD) and Fourier transformed infrared (FTIR). Additionally, microstructural and surface morphology were followed with different SeNPs contributions before and after fibroblast culturing. Moreover, Selenium dopant is affected Maximum roughness valley depth while it starts from 0.31⯵m at Au@0.0Se@PCL reaching 0.457⯵m at Au@12Se@PCL; however, after culturing starts from 0.3833⯵m reaching 0.41⯵m. Besides, the antibacterial activity was screened, showing the absence of inhibition zones in free selenium composition; however, it grows up reaching 8.3⯱â¯0.8, and 8.0⯱â¯0.8 for E. coli and S. aureus, respectively at the maximum contribution of selenium. SeNPs contributed composites show higher cell viability than Selenium free composite that it reaches its max in Au@8.0Se@PCL, recording 95.3⯱â¯2.3%. Composites show an excellent Wound dressing capability that its performance is directly proportional to selenium content. This significant enrichment of antibacterial activity and cell viability could recommend these composites for additional research in medical applications.
Sujet(s)
Thérapie laser , Nanofibres , Escherichia coli , Polyesters , Staphylococcus aureus , Cicatrisation de plaieRÉSUMÉ
Series of nanofibrous composites of polycaprolactone (PCL) were fabricated in different compositions of modified hydroxyapatite (HAP). The encapsulated HAP was co-doped with Ag/vanadate ions at different Ag contributions. XRD and FTIR techniques confirmed the powder and fibrous phase formation. Further, the morphological and mechanical behaviors of the electrospun nanofibrous scaffolds containing hydroxyapatite were investigated. The nanofibrous phases were biologically evaluated via studying contact angle, antibacterial, cell viability, and in vitro growth of human fibroblasts cell line (HFB4). It is obvious that silver ions cause gradual deviation in powder grains from wafer-like to cloudy grains. The maximum height of the roughness (Rt) ranged from 902.0 to 956.9 nm, while the valley depth of the roughness (Rv) ranged from 308.3 to 442.8 nm, for the lowest and the highest additional Ag ions for powdered phases. Moreover, the highest contribution of silver through the nanofibrous phases leads to the formation of lowest filaments size ranged from 0.07 to 0.53 µm. Further, the fracture strength was increased exponentially from 2.51 ± 0.35 MPa at zero concentration of silver ions up to 4.23 ± 0.64 MPa at 0.6 Ag/V-HAP@PCL. The fibrous phases were biologically evaluated in terms of antibacterial, cell viability, and in vitro growth of human fibroblasts cell line (HFB4). The nanofibrous composition of 0.8 Ag/V-HAP@PCL reached the maximum potential against E. coli and S. aureus and recorded 20.3 ± 1.1 and 19.8 ± 1.2 mm, respectively. This significant performance of the antibacterial activity and cell viability of co-doped HAP distributed through PCL could recommend these compositions for more research in biological applications, including wound healing.
RÉSUMÉ
The development of a scaffold matrix to promote wound healing is a critical requirement to improve the health care system. For this purpose, electrospun scaffolds of polycaprolactone (PCL) have been encapsulated with hydroxyapatite (HAP) doped with different contributions Ag ions. The obtained scaffolds have been investigated by XRD, FTIR and FESEM. It was shown that scaffolds were configured as cross-linked network with diameters around 0.6, 0.9, 2.1, and 2.5 µm for 0.0Ag/Fe-HAP@PCL, 0.4Ag/Fe-HAP@PCL, 0.6Ag/Fe-HAP@PCL, and 0.8Ag/Fe-HAP@PCL, respectively. Additionally, the composition of 0.8Ag/Fe-HAP@PCL exhibited the highest roughness average of 34 nm, while the inorganic root of co-dopant HAP recorded 44.8 nm. The mechanical properties have been investigated and showed that the maximum strain at break was about 129.31 ± 5.4% at no additional Ag ions, and reached its lowest value of 103.02 ± 3.5% at 0.2Ag/Fe-HAP@PCL. On the other hand, cell viability increased from 94.74 ± 4 to 98.9 ± 4% for 0.0Ag/Fe-HAP@PCL and 0.6Ag/Fe-HAP@PCL, respectively. Further, the antibacterial activity was investigated and exhibited that the inhibition zones of E. coli increased from 0.0 at 0.0Ag/Fe-HAP@PCL to 7.5 ± 1.3 mm for 0.8Ag/Fe-HAP@PCL. Moreover, the in vitro cell attachment showed that fibroblast cells proliferated and spread on the fibers' surface and through scaffolds' porosity.
Sujet(s)
Durapatite , Argent , Adhérence cellulaire , Escherichia coli , Polyesters , Ingénierie tissulaire , Structures d'échafaudage tissulairesRÉSUMÉ
Designing proper nanofibrous scaffolds for wound healing applications is a necessity for improving the health care system. Hydroxyapatite (HAP), zirconia (ZrO2), and graphene oxide (GO) nanosheets have been encapsulated in mono, di, or tri phases into nanofibrous scaffolds of polylactic acid (PLA). The structure of nanofibrous scaffolds is confirmed using XRD, XPS, while FESEM inspected the surface morphology. The surface morphology detection exhibited that the scaffolds have been formed in networked nanofibers with diameters from 1.19 to 2.38 to 0.59-1.42 µm, while the maximum height of the roughness increased from 610.4 to 809 nm for HAP@PLA and HAP/ZrO2/GO@PLA, respectively. The contact angle was measured and showed a decreasing trend from 101.2 ± 4.1° and 89.1 ± 5.4° for HAP@PLA and HAP/ZrO2/GO@PLA nanofibrous scaffolds. Moreover, the mechanical properties were examined and revealed that ZrO2 dopant induced a significant enhancement into the tensile strength, which increased from 3.49 ± 0.3 to 8.45 ± 1.1 MPa for the nanofibrous scaffolds of HAP@PLA and HAP/ZrO2/GO@PLA, respectively. The incorporation of ternary phases into PLA nanofibers promoted the cell viability to be around 98.2 ± 5%. The antibacterial potency has been investigated and showed that the activity increased to 69.2 ± 3.6 and 78.1 ± 4.5% against E. coli and S. aureus, respectively. Additionally, human fibroblasts proliferated on the surface and pores of nanofibrous scaffolds and significantly grown upon the compositional variation.
Sujet(s)
Nanofibres , Antibactériens/pharmacologie , Durapatite , Escherichia coli , Graphite , Humains , Polyesters , Staphylococcus aureus , Structures d'échafaudage tissulaires , Cicatrisation de plaieRÉSUMÉ
A combination of nanostructured zinc oxide (ZnO) or graphene oxide or both of them with cellulose acetate (CA) enhances a new functionality of nanofibers aiming to improve bio-composite materials for wound healing application. The obtained nanofibers have been investigated using XRD, FTIR, and FESEM. It was observed that the maximum height of the roughness increased from 253 to 651.9 nm for both GO and ZnO/GO in the powdered phase, while it plunged from 613 to 482 nm and developed to 801 nm for ZnO@CA, GO@CA, and ZnO/GO@CA, receptively. Further, the mechanical properties of the obtained scaffolds have been tested and displayed a tremendous variation of tensile strength from 5.44 ± 0.81 to 12.87 ± 0.93 and 8.82 ± 1.2 MPa, while the toughness increased from 23.29 ± 1.4 to 68.95 ± 4.5 and 57.75 ± 3.6 MJ/m3 for ZnO@CA, GO@CA and ZnO/GO@CA, receptively. Moreover, the cell viability was investigated and showed a progression of 97.38 ± 3.9% for ZnO/GO@CA. Furthermore, the adhesion of human fibroblasts cell line towards the obtained nanofibrous scaffolds were examined and displayed that cells were proliferated and spread considerably through the scaffolds, whereas their filopodia have followed the morphology of the fibers.
Sujet(s)
Nanofibres , Oxyde de zinc , Antibactériens/usage thérapeutique , Cellulose/analogues et dérivés , Graphite , Humains , Cicatrisation de plaieRÉSUMÉ
Skin wounds can lead to numerous complications with dangerous health consequences. In this work, magnetite nanoparticles were doped with different concentrations of antimicrobial silver (Ag) ions and incorporated into the electrospun nanofibrous ε-polycaprolactone (PCL) scaffolds. Nanoparticles and scaffolds with various Ag contents were characterized using a range of physicochemical techniques. Ag entered magnetite as cations and preferentially positioned at tetrahedral sites, introducing lattice distortions and topographic irregularities. Amorphization of the structure due to accommodation of Ag expanded the lattice in the bulk and contracted it on the surface, where broadened distribution of Fe-O coordinations was detected. Promoting spin canting and diminishing the double exchange interaction through altered distribution of ferric and ferrous ions, Ag softened the magnetism of magnetite. By making the nanoparticle structure more defective, Ag modified the interface with the polymer and promoted the protrusion of the nanoparticles from the surface of the polymeric nanofibers, thus increasing their roughness and hydrophilicity, with positive repercussions on cell adhesion and growth. Both the viability of human melanocytes and the antibacterial activity against E. coli and S. aureus increased with the concentration of Ag in the magnetite phase of the scaffolds. Skin wound healing rate in rats also increased in direct proportion with the concentration of Ag in the magnetite phase, and no abnormalities in the dermal and epidermal tissues were visible on day 10 in the treatment group. These results imply an excellent potential of these composite nanofibrous scaffolds for use as wound dressings and in other reconstructive skin therapies.
RÉSUMÉ
For wound healing applications, a scaffold of biocompatible/porous networks is crucial to support cell proliferation and spreading. Therefore,ϵ-polycaprolactone (PCL) nanofibrous scaffolds containing co-dopants of strontium/selenium in hydroxyapatite (HAP) were modified with different contributions of graphene oxide (GO) via the laser ablation technique. The obtained compositions were investigated using XRD, TEM and FESEM. It was evident that fiber diameters were in the range of 0.15-0.30µm and 0.35-0.83µm at the lowest and highest concentration of GO respectively, while the maximum height of the roughness progressed to 393 nm. The toughness behavior was promoted from 5.77 ± 0.21 to 9.16 ± 0.29 MJ m-3upon GO from the lowest to the highest contribution, while the maximum strain at break reached 148.1% ± 0.49% at the highest concentration of GO. The cell viability indicated that the fibrous scaffold was biocompatible. The investigation of the HFB4 cell attachments towards the fibrous compositions showed that with the increase of GO, cells tended to grow intensively through the scaffolds. Furthermore, the proliferation of cells was observed to be rooted in the porous structure and spreading on the surface of the scaffold. This progression of cells with an increase in GO content may provide a simple strategy not only to enhance the mechanical properties, but also to manipulate a nanofibrous scaffold with proper behaviors for biomedical applications.
Sujet(s)
Durapatite , Graphite , Nanofibres/composition chimique , Ingénierie tissulaire/méthodes , Structures d'échafaudage tissulaires/composition chimique , Adhérence cellulaire/effets des médicaments et des substances chimiques , Lignée cellulaire , Prolifération cellulaire/effets des médicaments et des substances chimiques , Survie cellulaire/effets des médicaments et des substances chimiques , Durapatite/composition chimique , Durapatite/pharmacologie , Graphite/composition chimique , Graphite/pharmacologie , Humains , Polyesters , Sélénium/composition chimique , Sélénium/pharmacologie , Strontium/composition chimique , Strontium/pharmacologieRÉSUMÉ
Recent advancements in the synthesis, properties, and applications of chitosan as the second after cellulose available biopolymer in nature were discussed in this review. A general overview of processing and production procedures from A to Z was highlighted. Chitosan exists in three polymorphic forms which differ in degree of crystallinity (α, ß, and γ). Thus, the degree of deacetylation, crystallinity, surface area, and molecular mass significantly affect most applications. Otherwise, the synthesis of chitosan nanofibers is suffering from many drawbacks that were recently treated by co-electrospun with other polymers such as polyvinyl alcohol (PVA), polyethylene oxide (PEO), and polycaprolactone (PCL). Ultimately, this review focuses on the area of new trend utilization of chitosan nanoparticles as nanospheres and nanocapsules, in cartilage and bone regenerative medicine. Owing to its biocompatibility, bioavailability, biodegradability, and costless synthesis, chitosan is a promising biopolymeric structure for water remediation, drug delivery, antimicrobials, and tissue engineering.
Sujet(s)
Bio-impression/méthodes , Chitosane/composition chimique , Chitosane/pharmacologie , Systèmes de délivrance de médicaments/méthodes , Nanocapsules/composition chimique , Nanofibres/composition chimique , Nanoparticules/composition chimique , Ingénierie tissulaire/méthodes , Anti-infectieux/pharmacologie , Matériaux biocompatibles/composition chimique , Nanocapsules/ultrastructure , Nanoparticules/ultrastructure , Solubilité , Structures d'échafaudage tissulaires/composition chimiqueRÉSUMÉ
Coating with hydroxyapatite (HAP) presents a mainstream strategy for rendering bioinert titanium implants bioactive. However, the low porosity of pure HAP coatings does not allow for the infiltration of the surface of the metallic implant with the host cells. Polymeric scaffolds do enable this osseointegration effect, but their bonding onto titanium presents a challenge because of the disparity in hydrophilicity. Here, we demonstrate the inability of a composite scaffold composed of carbonated HAP (CHAP) nanoparticles interspersed within electrospun ε-polycaprolactone (PCL) nanofibers to bind onto titanium. To solve this challenge, an intermediate layer of graphene nanosheets was deposited in a pulsed laser deposition process, which facilitated the bonding of the scaffold. The duration of the deposition of graphene (0, 5, 10, 15, and 20 min) and the thickness of its mesolayer affected numerous physical and chemical properties of the material, including the surface atomic proportion of carbon bonds, the orientation and interlinking of the polymeric nanofibers, and the surface roughness, which increased in direct proportion with the thickness of the graphene mesolayer. Because the polymeric scaffold did not adhere onto the surface of pure titanium, no cells were detected growing on it in vitro. In contrast, human fibroblasts adhered, spread, and proliferated well on all the substrates sputtered with both graphene and the composite scaffold. The orientations of cytoskeletal filopodia and lamellipodia were largely determined by the topographic orientation of the nanofibers and the geometry of the surface pores, attesting to the important effects that the presence of a scaffold has on the cellular behavior. The protection of titanium from corrosion in the simulated body fluid (SBF) was enhanced by coating with graphene and the composite scaffold, with the most superior resistance to the attack of the corrosive ions being exhibited by the substrate subjected to the shortest duration of the graphene deposition because of the highest atomic ratio of C-C to C-O bonds detected in it. Overall, some properties of titanium, such as roughness and wettability, were improved monotonously with an increase in the thickness of the graphene mesolayer, while others, such as cell viability and resistance to corrosion, required optimization, given that they were diminished at higher graphene mesolayer thicknesses. Nevertheless, every physical and chemical property of titanium analyzed was significantly improved by coating with graphene and the composite scaffold. This type of multilayer design evidently holds a great promise in the design of biomaterials for implants in orthopedics and tissue engineering.
Sujet(s)
Durapatite , Graphite , Matériaux revêtus, biocompatibles , Humains , Prothèses et implants , TitaneRÉSUMÉ
The development of a scaffold matrix that can inhibit bacterial infection and promote wound healing simultaneously is an essential demand to improve the health care system. Hydroxyapatite (HAP) doped with different concentrations of silver ions (Ag+) were incorporated into electrospun nanofibrous scaffolds of polycaprolactone (PCL) using the electrospinning technique. The formed phase was identified using XRD, while the morphological and roughness behavior were investigated using FESEM. It was shown that scaffolds were configured in randomly distributed nanofibers with diameters around of 0.19-0.40, 0.31-0.54, 1.36, 0.122-0.429 µm for 0.0Ag-HAP@PCL, 0.2Ag-HAP@PCL, 0.6Ag-HAP@PCL, and 0.8Ag-HAP@PCL, respectively. Moreover, the maximum roughness peak height increased significantly from 179 to 284 nm, with the lowest and highest contributions of Ag. The mechanical properties were examined and displayed that the tensile strength increased from 3.11 ± 0.21 MPa to its highest value at 3.57 ± 0.31 MPa for 0.4Ag-HAP@PCL. On the other hand, the cell viability also was enhanced with the addition of Ag and improved from 97.1 ± 4.6% to be around 102.3 ± 3.1% at the highest contribution of Ag. The antibacterial activity was determined, and the highest imbibition zones were achieved at the highest Ag dopant to be 12.5 ± 1.1 mm and 11.4 ± 1.5 mm against E. coli and S. aureus. The in vitro cell proliferation was observed through human fibroblasts cell lone (HFB4) and illustrated that cells were able to grow and spread not only on the fibers' surface but also, they were spreading and adhered through the deep pores.
Sujet(s)
Durapatite , Nanofibres , Antibactériens/pharmacologie , Bandages , Adhérence cellulaire , Escherichia coli , Humains , Polyesters , Argent , Staphylococcus aureus , Structures d'échafaudage tissulairesRÉSUMÉ
The use of ions as therapeutic agents has the potential to minimize the use of small-molecule drugs and biologics for the same purpose, thus providing a potentially more economic and less adverse means of treating, ameliorating or preventing a number of diseases. Hydroxyapatite (HAp) is a solid compound capable of accommodating foreign ions with a broad range of sizes and charges and its properties can dramatically change with the incorporation of these ionic additives. While most ionic substitutes in HAp have been monatomic cations, their lesser atomic weight, higher diffusivity, chaotropy and a lesser residence time on surfaces theoretically makes them prone to exert a lesser influence on the material/cell interaction than the more kosmotropic oxyanions. Selenite ion as an anionic substitution in HAp was explored in this study for its ability to affect the short-range and the long-range crystalline symmetry and solubility as well as for its ability to affect the osteoclast activity. We combined microstructural, crystallographic and spectroscopic analyses with quantum mechanical calculations to understand the structural effects of doping HAp with selenite. Integration of selenite ions into the crystal structure of HAp elongated the crystals along the c-axis, but isotropically lowered the crystallinity. It also increased the roughness of the material in direct proportion with the content of the selenite dopant, thus having a potentially positive effect on cell adhesion and integration with the host tissue. Selenite in total acted as a crystal structure breaker, but was also able to bring about symmetry at the local and global scales within specific concentration windows, indicating a variety of often mutually antagonistic crystallographic effects that it can induce in a concentration-dependent manner. Experimental determination of the lattice strain coupled with ab initio calculations on three different forms of carbonated HAp (A-type, B-type, AB-type) demonstrated that selenite ions initially substitute carbonates in the crystal structure of carbonated HAp, before substituting phosphates at higher concentrations. The most energetically favored selenite-doped HAp is of AB-type, followed by the B-type and only then by the A-type. This order of stability was entailed by the variation in the geometry and orientation of both the selenite ion and its neighboring phosphates and/or carbonates. The incorporation of selenite in different types of carbonated HAp also caused variations of different thermodynamic parameters, including entropy, enthalpy, heat capacity, and the Gibbs free energy. Solubility of HAp accommodating 1.2 wt% of selenite was 2.5 times higher than that of undoped HAp and the ensuing release of the selenite ion was directly responsible for inhibiting RAW264.7 osteoclasts. Dose-response curves demonstrated that the inhibition of osteoclasts was directly proportional to the concentration of selenite-doped HAp and to the selenite content in it. Meanwhile, selenite-doped HAp had a significantly less adverse effect on osteoblastic K7M2 and MC3T3-E1 cells than on RAW264.7 osteoclasts. The therapeutically promising osteoblast vs. osteoclast selectivity of inhibition was absent when the cells were challenged with undoped HAp, indicating that it is caused by selenite ions in HAp rather than by HAp alone. It is concluded that like three oxygens building the selenite pyramid, the coupling of (1) experimental materials science, (2) quantum mechanical modeling and (3) biological assaying is a triad from which a deeper understanding of ion-doped HAp and other biomaterials can emanate.
Sujet(s)
Durapatite , Ostéoblastes , Ostéoclastes , Acide sélénieuxRÉSUMÉ
Composite structures are at the frontier of materials science and engineering and polymeric/ceramic composites present one of their most prospective subsets. Prior studies have shown both improvements and deteriorations of properties of polymers upon the addition of ceramic phases to them, but not many studies have dealt with the direct comparison of chemically distinct inorganic additives. The goal of this study was to compare the properties of ε-polycaprolactone (PCL) thin films supplemented with alumina, graphene, carbonated hydroxyapatite or titania particles, individually, in identical amounts (12 wt%). The composite films were analyzed for their phase composition, grain size, morphology, surface roughness, porosity, cell response, mechanical properties and electrochemical performance. Each additive imparted one or more physical or biological properties onto PCL better than others. Thus, alumina increased the microhardness of the films better than any other additive, with the resulting values exceeding 10 MPa. It also led to the formation of a composite with the least porosity and the greatest stability to degradation in simulated body fluid based on open circuit potential (OCP) measurements and electrochemical impedance spectroscopy (EIS). Titania made the surface of PCL roughest, which in combination with its high porosity explained why it was the most conducive to the growth of human fibroblasts, alongside being most prone to degradation in wet, corrosive environments and having the highest Poisson's ratio. Graphene, in contrast, made the surface of PCL smoothest and the bulk structure most porous, but also most conductive, with the OCP of -37 mV. The OCP of PCL supplemented with carbonated hydroxyapatite had the highest OCP of -134 mV and also the highest mechanical moduli, including the longitudinal (781 MPa), the shear (106 MPa), the bulk (639 MPa), and the elastic (300 MPa). The only benefit of the deposition of multilayered PCL films supplemented with all four inorganic additives was to enable a relatively high resistance to degradation. This study demonstrates that the properties of thin PCL films could be effectively optimized through the simple choice of appropriate inorganic additives dispersed in them. There is no single additive that proves ideal for improving all the properties of interest in PCL thin films, but their choice should be adjusted to the actual application. One such method of compositional optimization could prove crucial in the effort to develop biocomposites for superior performance in tissue engineering applications.