Crise global da atenção primária à saúde / Global crisis in primary health care / Crisis global de la atención primaria

Enquanto no Norte Global se discute uma crise na Atenção Primária à Saúde, a maioria dos países nunca chegou a constituir sistemas de saúde baseados propriamente numa atenção primária robusta. Nesse cenário, o Brasil apresenta uma tendência mais favorável, com conquistas importantes para a atenção primária e a medicina de família e comunidade nos últimos dez anos. Restam desafios a serem superados para que o Sistema Único de Saúde alcance níveis satisfatórios de acesso a seus serviços, com profissionais adequadamente formados e valorizados pela população.

While the Global North is discussing a crisis in primary health care, the majority of countries have never managed to establish health systems based on robust primary care. Brazil presents a more favorable trend, with important achievements for primary care and family practice over the last ten years. There are still challenges to be overcome so that the Unified Health System achieves satisfactory levels of access to its services, with professionals who are properly trained and valued by the public.

Mientras que en el Norte Global se habla de una crisis de la atención primaria, la mayoría de los países nunca han creado realmente sistemas sanitarios basados en una atención primaria robusta. Brasil, muestra una tendencia más favorable, con importantes logros para la atención primaria y la medicina familiar y comunitaria en los últimos diez años. Aún quedan retos por superar para que el Sistema Único de Salud alcance niveles satisfactorios de acceso a sus servicios, con profesionales debidamente formados y valorados por la población.

Leishmania (Sauroleishmania) tarentolae versus pathogenic species: comparative evaluation of protease activity, glycoconjugates, resistance to complement and metabolome composition.

BACKGROUND: Leishmania tarentolae is a non-pathogenic species found in lizards representing an important model for Leishmania biology. However, several aspects of this Sauroleishmania remain unknown to explain its low level of virulence. OBJECTIVES: We reported several aspects of L. tarentolae biology including glycoconjugates, proteolytic activities and metabolome composition in comparison to pathogenic species (Leishmania amazonensis, Leishmania braziliensis, Leishmania infantum and Leishmania major). METHODS: Parasites were cultured for extraction and purification of lipophosphoglycan (LPG), immunofluorescence probing with anti-gp63 and resistance against complement. Parasite extracts were also tested for proteases activity and metabolome composition. FINDINGS: Leishmania tarentolae does not express LPG on its surface. It expresses gp63 at lower levels compared to pathogenic species and, is highly sensitive to complement-mediated lysis. This species also lacks intracellular/extracellular activities of proteolytic enzymes. It has metabolic differences with pathogenic species, exhibiting a lower abundance of metabolites including ABC transporters, biosynthesis of unsaturated fatty acids and steroids, TCA cycle, glycine/serine/threonine metabolism, glyoxylate/dicarboxylate metabolism and pentose-phosphate pathways. MAIN CONCLUSIONS: The non-pathogenic phenotype of L. tarentolae is associated with alterations in several biochemical and molecular features. This reinforces the need of comparative studies between pathogenic and non-pathogenic species to elucidate the molecular mechanisms of virulence during host-parasite interactions.

Long-term occurrence of multiple antimicrobial drug resistant Klebsiella pneumoniae isolates harboring virulent potential in a tertiary hospital from Brazil.

Klebsiella pneumoniae strains are globally associated with a plethora of opportunistic and severe human infections and are known to spread genes conferring antimicrobial resistance. Some strains harbor virulence determinants that enable them to cause serious disease in any patient, both in the hospital and in the community. The aim of this study was to determine the frequency of antimicrobial resistance and virulence traits (by gene detection and string test) among 83 K. pneumoniae isolates obtained from patient cultures of a scholar tertiary hospital in the Midwestern Brazil (Brasília, DF). Antimicrobial susceptibility analysis showed that 94% (78/83) of the isolates presented one of the following resistance profiles: resistant (R, 39), multidrug-resistant (MDR, 29), or extensively drug-resistant (XDR, 10). Several MDR and XDR strains harbored multiple virulence genes and displayed hypermucoviscous phenotype. These characteristics were observed among isolates obtained throughout all the sample collection period (2013 - 2017). The K2 serotype gene, a molecular marker of hypervirulence, was detected in three isolates, one of which classified as XDR. Sequence typing revealed the occurrence of isolates belonged to high-risk (ST13) and multiple resistance-spreading clones (ST105). Thus, our findings showed the occurrence of virulent potential isolates that also presented MDR/XDR phenotypes from 2013 to 2015. This study also indicates the probable convergence of virulence and resistance since at least 2013 in Brazil.

Thoracic Spine Mobility and Posture: Correlation and Predictive Values in Physically Independent Older Adults.

The posture undergoes changes during aging and may serve as a marker for the evaluation of the thoracic spine. This study aimed to correlate the variables for the evaluation of thoracic spine mobility and propose predictive equation models from the measurements of the thoracic Schober test and the digital inclinometer in older adults. The mobility of thoracic flexion and extension by levels (T1, T8 and T12) of 41 older adult subjects (66 ± 7 years) was quantified with a digital inclinometer (degrees) and Schober's test (cm). There was a moderate positive correlation between the digital inclinometer and the Schober test at T1 (r = .69), T12 (r = .60), and total flexion levels T1 to T12 (r = .74). Simple linear regression equations showed that thoracic Schober predicts thoracic mobility measures for these same levels. Moderate to strong correlations were observed between the inclinometer and the Schober Test measurements. The development of predictive equation models based on the thoracic Schober test could potentially enhance the ability to predict spinal mobility in physically independent older adults.

Leishmania (Sauroleishmania) tarentolae versus pathogenic species: comparative evaluation of protease activity, glycoconjugates, resistance to complement and metabolome composition

BACKGROUND Leishmania tarentolae is a non-pathogenic species found in lizards representing an important model for Leishmania biology. However, several aspects of this Sauroleishmania remain unknown to explain its low level of virulence. OBJECTIVES We reported several aspects of L. tarentolae biology including glycoconjugates, proteolytic activities and metabolome composition in comparison to pathogenic species (Leishmania amazonensis, Leishmania braziliensis, Leishmania infantum and Leishmania major). METHODS Parasites were cultured for extraction and purification of lipophosphoglycan (LPG), immunofluorescence probing with anti-gp63 and resistance against complement. Parasite extracts were also tested for proteases activity and metabolome composition. FINDINGS Leishmania tarentolae does not express LPG on its surface. It expresses gp63 at lower levels compared to pathogenic species and, is highly sensitive to complement-mediated lysis. This species also lacks intracellular/extracellular activities of proteolytic enzymes. It has metabolic differences with pathogenic species, exhibiting a lower abundance of metabolites including ABC transporters, biosynthesis of unsaturated fatty acids and steroids, TCA cycle, glycine/serine/threonine metabolism, glyoxylate/dicarboxylate metabolism and pentose-phosphate pathways. MAIN CONCLUSIONS The non-pathogenic phenotype of L. tarentolae is associated with alterations in several biochemical and molecular features. This reinforces the need of comparative studies between pathogenic and non-pathogenic species to elucidate the molecular mechanisms of virulence during host-parasite interactions.

Challenges of a two-phase orthodontic treatment of a mandibular lateral incisor-canine transposition and a maxillary palatally displaced canine: A case report.

INTRODUCTION: The management of eruption disturbances in orthodontics may be challenging and requires a careful diagnosis and treatment planning. This case report discusses the challenges of a two-phase orthodontic treatment of a patient presenting with a dental eruption pattern anomaly. PATIENT CONCERNS: A 10-year-old boy was presented with no complaints for a routine orthodontic evaluation during mixed dentition. PRIMARY DIAGNOSES: The patient was diagnosed with a skeletal Class I malocclusion with unilateral posterior crossbite, incomplete mandibular lateral incisor-canine transposition and a unilateral maxillary ectopic canine. INTERVENTIONS: Phase 1 started with rapid maxillary expansion to correct maxillary constriction and the ectopic eruption of the right maxillary canine. In the mandibular arch, phase 1 included the extraction of the left primary lateral incisor and canine, alignment of the left permanent lateral incisor and orthodontic traction of the left permanent canine. The duration of phase 1 was 14 months. Phase 2 involved a comprehensive course of orthodontic treatment and started when the patient was aged 13 years. This phase lasted 18 months. RESULTS: An adequate dental occlusion was obtained, and the treatment results were stable after an 18-month follow-up. CONCLUSION: In this case, the early diagnosis of the dental anomalies was valuable as it allowed an early intervention to be undertaken, which resulted in overall treatment simplification and potentially minimised the adverse effects. This case report reinforces the importance of a careful follow-up during mixed dentition.

Energetic responses of head-out water immersion at different temperatures during post-exercise recovery and its consequence on anaerobic mechanical power.

PURPOSE: While exercise recovery may be beneficial from a physiological point of view, it may be detrimental to subsequent anaerobic performance. To investigate the energetic responses of water immersion at different temperatures during post-exercise recovery and its consequences on subsequent anaerobic performance, a randomized and controlled crossover experimental design was performed with 21 trained cyclists. METHOD: Participants were assigned to receive three passive recovery strategies during 10 min after a Wingate Anaerobic Test (WAnT): control (CON: non-immersed condition), cold water immersion (CWI: 20 â„ƒ), and hot water immersion (HWI: 40 â„ƒ). Blood lactate, cardiorespiratory, and mechanical outcomes were measured during the WAnT and its recovery. Time constant (τ), asymptotic value, and area under the curve (AUC) were quantified for each physiologic parameter during recovery. After that, a second WAnT test and 10-min recovery were realized in the same session. RESULTS: Regardless the water immersion temperature, water immersion increased [Formula: see text] (+ 18%), asymptote ([Formula: see text]+ 16%, [Formula: see text] + 13%, [Formula: see text] + 17%, HR + 16%) and AUC ([Formula: see text]+ 27%, [Formula: see text] + 18%, [Formula: see text] + 20%, HR + 25%), while decreased [Formula: see text] (- 33%). There was no influence of water immersion on blood lactate parameters. HWI improved the mean power output during the second WAnT (2.2%), while the CWI decreased 2.4% (P < 0.01). CONCLUSION: Independent of temperature, water immersion enhanced aerobic energy recovery without modifying blood lactate recovery. However, subsequent anaerobic performance was increased only during HWI and decreased during CWI. Despite higher than in other studies, 20 °C effectively triggered physiological and performance responses. Water immersion-induced physiological changes did not predict subsequent anaerobic performance.

Stress response of Rhamdia quelen to the interaction stocking density - Feeding regimen.

This study aimed to verify the effect of different feeding and stocking conditions during 14 days on the gene expression of several hormones and enzymes related to the stress cascade and metabolic parameters in silver catfish Rhamdia quelen under the following experimental conditions: 1) fed at low stocking density (2.5 kg m-3, LSD-F); 2) fed at high stocking density (32 kg m-3, HSD-F); 3) food-deprived at LSD (LSD-FD); and 4) food-deprived at HSD (HSD-FD). Fish from LSD-F and HSD-F groups were fed daily (1 % of their body mass), while fish from food-deprived groups (LSD-FD and HSD-FD) were not fed during the experimental time. Plasma metabolic parameters (glucose, lactate, triglycerides, and proteins) and hepatosomatic index (HSI) were evaluated. In addition, mRNA expression of genes related to the stress axis (crh, pomca, pomcb, nr3c2, star, hsd11b2 and hsd20b), heat shock protein family (hsp90 and hspa12a), sodium-dependent noradrenaline transporter (slc6a2), and growth axis (gh and igf1) were also assessed. Specific growth rate and HSI decreased in food-deprived fish regardless of stocking density. The HSD-FD group showed weight loss compared to the HSD-F, LSD-F, and LSD-FD groups. Plasma glucose and triglycerides were reduced in food-deprived groups, while lactate and protein levels did not change. The expression of key players of the stress response (crh, pomca, pomcb, hsd11b2, nr3c2, and hsp90b) and growth (gh and igf1) pathways were differently regulated depending on the experimental condition, whereas no statistical difference between treatments was found for hsd20b, scl6a2, hspa12a, and star mRNAs expression. This study suggests that LSD acts as a stressor affecting negatively the physiological status of fed fish, as demonstrated by the reduction in growth rates, altered metabolic orchestration, and a higher crh mRNA expression. In addition, food deprivation also increased mRNA expression of other assessed genes (nr3c2, hsp90b, pomca, and pomcb) in fish from the HSD group, indicating higher responsiveness to stress in this stocking density when combined with food deprivation.

Diálogos interinstitucionais na judicialização da saúde como estratégia de sustentabilidade do SUS / Inter-institutional dialogues in the judicialization of health as a sustainability strategy for the SUS

RESUMO Este estudo teve por objetivo explorar as estratégias dialógicas adotadas na condução dos processos de saúde ajuizados pela Defensoria Pública no município de Barreiras-BA, de modo a demonstrar que o caráter complexo e policêntrico da judicialização exige uma atuação articulada entre os atores envolvidos, voltada à garantia de sustentabilidade do Sistema Único de Saúde. Adotou-se, para tanto, metodologia descritiva e retrospectiva, com foco em base documental. Os dados foram obtidos a partir de uma relação de processos elaborada pela Defensoria Pública atuante no município, seguida da consulta da íntegra dos autos no site do Tribunal de Justiça da Bahia. Foram analisados 94 processos, ajuizados entre janeiro de 2019 a dezembro de 2021. A análise das práticas dialógicas teve como referencial a Teoria da Ação Comunicativa de Habermas. Concluiu-se que a Defensoria possui protagonismo na etapa pré-processual, buscando, em um agir comunicativo, a resolução extrajudicial dos conflitos. Por outro lado, a postura dos órgãos destinatários demonstrou falha na gestão pública da saúde, que age de forma estratégica e não tem a resolução extrajudicial como prioridade. Restou evidente, ademais, a necessidade de fortalecimento do Núcleo de Apoio Técnico do Poder Judiciário (NAT-Jus), ainda pouco consolidado no Judiciário baiano.

ABSTRACT This study aims to explore the dialogic strategies adopted in the conduct of health processes filed by the Public Defender's Office in Barreiras-BA, in order to demonstrate that the complex and polycentric character of judicialization requires an articulated action between the actors involved, aimed at guaranteeing the sustainability of the Unified Health System. A descriptive and retrospective methodology was adopted, focusing on a documental basis. The data were obtained from a list of cases prepared by the Public Defender's Office operating in the municipality, followed by consultation of the entirety of the records on the website of the Court of Justice of Bahia. 94 cases were analyzed, filed between January 2019 and December 2021. The analysis of dialogic practices was based on Habermas' Theory of Communicative Action. It was concluded that the Defender's Office has a leading role in the pre-procedural stage, seeking, in a communicative action, the extrajudicial resolution of conflicts. On the other hand, the attitude of Organs recipient bodies showed a failure in public health management, which acts strategically and does not have extrajudicial resolution as a priority. Furthermore, the need to strengthen the NAT-Jus remained evident, which is still poorly consolidated in the Bahian Judiciary.

[Incorporation of telehealth in primary healthcare and associated factors in Brazil]. / Incorporação de telessaúde na atenção primária à saúde no Brasil e fatores associados.

The study aimed to analyze the Brazil Networks Telehealth Program in the context of primary healthcare (PHC) in Brazil and to identify related factors. This cross-sectional study used data from the second cycle of the Program for Improvement of Access and Quality of Basic Healthcare (PMAQ-AB). The sample consisted of 29,756 healthcare teams who joined the program voluntarily. Independent variables included contextual characteristics (region and population size), healthcare unit (type, telephone access, broadband, number of physicians and nurses, consultation offices, community health workers' room, meeting room, existence of telehealth) and health team characteristics (institutional support). Crude and Poisson regression-adjusted analyses assessed which variables are associated with greater use of telehealth. Prevalence of use of telehealth was 32.7% in the total sample and 73.3% among teams with the Program implemented. Tele-education was the most frequently used modality. Higher rates of prevalence of use were found in the South and Southeast of Brazil, in municipalities with up to 30,000 inhabitants, with telehealth implemented in the unit, with presence of at least one physician, and with at least one telephone available in the service. Institutional support had a 40% positive impact on prevalence of use of telehealth. Infrastructure variables such as Internet and availability of rooms with different purposes appeared not to significantly impact the use of these technologies, indicating that institutional support and the implementation of telehealth itself in the unit are more important for increasing adherence to the Program.

O estudo teve como objetivo analisar a utilização do Programa Nacional Telessaúde Brasil Redes, no âmbito da atenção primária à saúde no Brasil, identificando os fatores relacionados. Estudo transversal a partir da base de dados do segundo ciclo do Programa Nacional de Melhoria do Acesso e da Qualidade da Atenção Básica (PMAQ-AB). A amostra foi composta por 29.756 equipes de saúde que, voluntariamente, aderiram ao programa. As variáveis independentes abrangeram características contextuais (região e porte populacional), da unidade de saúde (tipo, acesso a telefone, banda larga, número de médicos e enfermeiros, consultórios, sala de agentes comunitários de saúde (ACS), sala de reuniões, existência de telessaúde) e da equipe de saúde (apoio institucional). Análises bruta e ajustada por meio de regressão de Poisson avaliaram quais variáveis são associadas ao maior uso do telessaúde. A prevalência de utilização do telessaúde foi de 32,7% no total da amostra e 73,3% entre equipes com o programa implantado. Teleducação foi a modalidade mais frequentemente usada. Maiores prevalências de utilização foram encontradas das regiões Sul e Sudeste, em municípios com até 30 mil habitantes, com telessaúde implantado na unidade, presença de pelo menos um médico e disponibilidade de pelo menos um telefone no estabelecimento. O apoio institucional teve impacto positivo em 40% na prevalência de utilização do telessaúde. Variáveis de estrutura, como Internet e disponibilidade de salas com distintas finalidades, parecem não impactar significativamente a utilização dessas tecnologias, sinalizando que fatores como apoio institucional e a própria implantação do telessaúde na unidade são mais importantes para aumentar a adesão ao programa.

El estudio tuvo como objetivo analizar la utilización del Programa Nacional Telesalud Brasil Redes, en el ámbito de la atención primaria en salud en Brasil, identificando los factores relacionados. Estudio transversal a partir de la base de datos del 2º ciclo del Programa Nacional de Mejoría de Acceso y Calidad de la Atención Básica (PMAQ-AB). La muestra estuvo compuesta por 29.756 equipos de salud que voluntariamente se adhirieron al programa. Las variables independientes abarcaron características contextuales (región y tamaño poblacional), de la unidad de salud (tipo, acceso a teléfono, banda ancha, número de médicos y enfermeros, consultorios, sala de agentes comunitarios de salud, sala de reuniones, existencia de telesalud) y del equipo de salud (apoyo institucional). El análisis bruto y ajustado mediante regresión de Poisson evaluaron qué variables se asocian a un mayor uso de telesalud. La prevalencia de utilización de telesalud fue de un 32,7% en el total de la muestra y un 73,3% entre equipos con el programa implantado. Teleducación fue la modalidad más frecuentemente usada. Se encontraron mayores prevalencias de utilización en las regiones Sur y Sudeste, en municipios con hasta 30.000 habitantes, con telesalud implantada en la unidad, presencia de por lo menos un médico y disponibilidad de por lo menos un teléfono en el establecimiento. El apoyo institucional tuvo un impacto positivo en un 40% de la prevalencia de utilización de la telesalud. Variables de estructura como Internet y disponibilidad de salas con distintas finalidades parecen no impactar significativamente en la utilización de estas tecnologías, señalando que los factores como el apoyo institucional y la propia implantación de la telesalud en la unidad son más importantes para aumentar la adhesión al programa.

Effect of early treatment with fluvoxamine on risk of emergency care and hospitalisation among patients with COVID-19: the TOGETHER randomised, platform clinical trial.

BACKGROUND: Recent evidence indicates a potential therapeutic role of fluvoxamine for COVID-19. In the TOGETHER trial for acutely symptomatic patients with COVID-19, we aimed to assess the efficacy of fluvoxamine versus placebo in preventing hospitalisation defined as either retention in a COVID-19 emergency setting or transfer to a tertiary hospital due to COVID-19. METHODS: This placebo-controlled, randomised, adaptive platform trial done among high-risk symptomatic Brazilian adults confirmed positive for SARS-CoV-2 included eligible patients from 11 clinical sites in Brazil with a known risk factor for progression to severe disease. Patients were randomly assigned (1:1) to either fluvoxamine (100 mg twice daily for 10 days) or placebo (or other treatment groups not reported here). The trial team, site staff, and patients were masked to treatment allocation. Our primary outcome was a composite endpoint of hospitalisation defined as either retention in a COVID-19 emergency setting or transfer to tertiary hospital due to COVID-19 up to 28 days post-random assignment on the basis of intention to treat. Modified intention to treat explored patients receiving at least 24 h of treatment before a primary outcome event and per-protocol analysis explored patients with a high level adherence (>80%). We used a Bayesian analytic framework to establish the effects along with probability of success of intervention compared with placebo. The trial is registered at ClinicalTrials.gov (NCT04727424) and is ongoing. FINDINGS: The study team screened 9803 potential participants for this trial. The trial was initiated on June 2, 2020, with the current protocol reporting randomisation to fluvoxamine from Jan 20 to Aug 5, 2021, when the trial arms were stopped for superiority. 741 patients were allocated to fluvoxamine and 756 to placebo. The average age of participants was 50 years (range 18-102 years); 58% were female. The proportion of patients observed in a COVID-19 emergency setting for more than 6 h or transferred to a teritary hospital due to COVID-19 was lower for the fluvoxamine group compared with placebo (79 [11%] of 741 vs 119 [16%] of 756); relative risk [RR] 0·68; 95% Bayesian credible interval [95% BCI]: 0·52-0·88), with a probability of superiority of 99·8% surpassing the prespecified superiority threshold of 97·6% (risk difference 5·0%). Of the composite primary outcome events, 87% were hospitalisations. Findings for the primary outcome were similar for the modified intention-to-treat analysis (RR 0·69, 95% BCI 0·53-0·90) and larger in the per-protocol analysis (RR 0·34, 95% BCI, 0·21-0·54). There were 17 deaths in the fluvoxamine group and 25 deaths in the placebo group in the primary intention-to-treat analysis (odds ratio [OR] 0·68, 95% CI: 0·36-1·27). There was one death in the fluvoxamine group and 12 in the placebo group for the per-protocol population (OR 0·09; 95% CI 0·01-0·47). We found no significant differences in number of treatment emergent adverse events among patients in the fluvoxamine and placebo groups. INTERPRETATION: Treatment with fluvoxamine (100 mg twice daily for 10 days) among high-risk outpatients with early diagnosed COVID-19 reduced the need for hospitalisation defined as retention in a COVID-19 emergency setting or transfer to a tertiary hospital. FUNDING: FastGrants and The Rainwater Charitable Foundation. TRANSLATION: For the Portuguese translation of the abstract see Supplementary Materials section.

Instruments used to measure the quality of life related to oral health: integrated review / Instrumentos utilizados para medir a qualidade de vida relacionada à saúde oral: revisão integrativa

ABSTRACT This study aimed to identify the instruments used to measure the quality of life related to oral health (HRQoL) as well as the measures that were adapted for the Portuguese language of Brazil. This is an integrative review whose inclusion criteria were papers in Portuguese, English and Spanish, published and indexed in databases Medline (PubMed) and Virtual Health Library (BVS). One thousand two hundred and sixty papers submitted, fifty-five were considered eligible for review, from which the following information was extracted: instrument; composition; community; sample size; measured domains / dimensions; and author, year, country. Subsequently, the data related to the translation and cultural adaptation processes for the Portuguese language of Brazil were collected in order to evaluate the psychometric properties of each study. The studies of this review show that the HRQoL theme has remained in evidence since the creation of the first instruments and seems not to be close to being exhausted. Of the 36 instruments presented, only 17 articles were identified in the databases assessed for cross-cultural adaptation to Brazilian Portuguese and validation of psychometric properties. Thus, despite the enormous dissemination of instruments, the need for translation, adaptation in the language and analysis of psychometric properties was pointed out in this study.

RESUMO Objetivou-se identificar os instrumentos utilizados para medir a qualidade de vida relacionada à saúde bucal (QVRSB) bem como as medidas que foram adaptadas para a língua portuguesa do Brasil. Trata-se de uma revisão integrativa, cujos critérios de inclusão foram artigos nos idiomas português, inglês e espanhol, publicados e indexados nas bases de dados Medline (PubMed) e Biblioteca Virtual em Saúde. Dentre os 1.260 artigos levantados, 55 foram considerados elegíveis para a revisão dos quais se extraíram as seguintes informações: instrumento; composição; público-alvo; tamanho da amostra; domínios/dimensões medidas; e autor, ano, local. Posteriormente, foram levantados os dados referentes aos processos de tradução e adaptação cultural para o idioma português do Brasil com a finalidade de avaliar os dados referentes às propriedades psicométricas de cada estudo. Os estudos desta revisão mostram que a temática QVRSB se mantem em evidência desde a criação dos primeiros instrumentos e parece não estar perto de se esgotar. Dos 36 instrumentos apresentados, apenas 17 artigos foram identificados nas bases de dados avaliadas quanto a adaptação transcultural para o idioma português do Brasil e validação das propriedades psicométricas. Assim, apesar da enorme difusão de instrumentos, a necessidade de tradução, adaptação no idioma e análise das propriedades psicométricas foi apontada neste estudo.

Incorporação de telessaúde na atenção primária à saúde no Brasil e fatores associados / Incorporation of telehealth in primary healthcare and associated factors in Brazil / Incorporación de la telesalud en la atención primaria en salud en Brasil y factores asociados

O estudo teve como objetivo analisar a utilização do Programa Nacional Telessaúde Brasil Redes, no âmbito da atenção primária à saúde no Brasil, identificando os fatores relacionados. Estudo transversal a partir da base de dados do segundo ciclo do Programa Nacional de Melhoria do Acesso e da Qualidade da Atenção Básica (PMAQ-AB). A amostra foi composta por 29.756 equipes de saúde que, voluntariamente, aderiram ao programa. As variáveis independentes abrangeram características contextuais (região e porte populacional), da unidade de saúde (tipo, acesso a telefone, banda larga, número de médicos e enfermeiros, consultórios, sala de agentes comunitários de saúde (ACS), sala de reuniões, existência de telessaúde) e da equipe de saúde (apoio institucional). Análises bruta e ajustada por meio de regressão de Poisson avaliaram quais variáveis são associadas ao maior uso do telessaúde. A prevalência de utilização do telessaúde foi de 32,7% no total da amostra e 73,3% entre equipes com o programa implantado. Teleducação foi a modalidade mais frequentemente usada. Maiores prevalências de utilização foram encontradas das regiões Sul e Sudeste, em municípios com até 30 mil habitantes, com telessaúde implantado na unidade, presença de pelo menos um médico e disponibilidade de pelo menos um telefone no estabelecimento. O apoio institucional teve impacto positivo em 40% na prevalência de utilização do telessaúde. Variáveis de estrutura, como Internet e disponibilidade de salas com distintas finalidades, parecem não impactar significativamente a utilização dessas tecnologias, sinalizando que fatores como apoio institucional e a própria implantação do telessaúde na unidade são mais importantes para aumentar a adesão ao programa.

The study aimed to analyze the Brazil Networks Telehealth Program in the context of primary healthcare (PHC) in Brazil and to identify related factors. This cross-sectional study used data from the second cycle of the Program for Improvement of Access and Quality of Basic Healthcare (PMAQ-AB). The sample consisted of 29,756 healthcare teams who joined the program voluntarily. Independent variables included contextual characteristics (region and population size), healthcare unit (type, telephone access, broadband, number of physicians and nurses, consultation offices, community health workers' room, meeting room, existence of telehealth) and health team characteristics (institutional support). Crude and Poisson regression-adjusted analyses assessed which variables are associated with greater use of telehealth. Prevalence of use of telehealth was 32.7% in the total sample and 73.3% among teams with the Program implemented. Tele-education was the most frequently used modality. Higher rates of prevalence of use were found in the South and Southeast of Brazil, in municipalities with up to 30,000 inhabitants, with telehealth implemented in the unit, with presence of at least one physician, and with at least one telephone available in the service. Institutional support had a 40% positive impact on prevalence of use of telehealth. Infrastructure variables such as Internet and availability of rooms with different purposes appeared not to significantly impact the use of these technologies, indicating that institutional support and the implementation of telehealth itself in the unit are more important for increasing adherence to the Program.

El estudio tuvo como objetivo analizar la utilización del Programa Nacional Telesalud Brasil Redes, en el ámbito de la atención primaria en salud en Brasil, identificando los factores relacionados. Estudio transversal a partir de la base de datos del 2º ciclo del Programa Nacional de Mejoría de Acceso y Calidad de la Atención Básica (PMAQ-AB). La muestra estuvo compuesta por 29.756 equipos de salud que voluntariamente se adhirieron al programa. Las variables independientes abarcaron características contextuales (región y tamaño poblacional), de la unidad de salud (tipo, acceso a teléfono, banda ancha, número de médicos y enfermeros, consultorios, sala de agentes comunitarios de salud, sala de reuniones, existencia de telesalud) y del equipo de salud (apoyo institucional). El análisis bruto y ajustado mediante regresión de Poisson evaluaron qué variables se asocian a un mayor uso de telesalud. La prevalencia de utilización de telesalud fue de un 32,7% en el total de la muestra y un 73,3% entre equipos con el programa implantado. Teleducación fue la modalidad más frecuentemente usada. Se encontraron mayores prevalencias de utilización en las regiones Sur y Sudeste, en municipios con hasta 30.000 habitantes, con telesalud implantada en la unidad, presencia de por lo menos un médico y disponibilidad de por lo menos un teléfono en el establecimiento. El apoyo institucional tuvo un impacto positivo en un 40% de la prevalencia de utilización de la telesalud. Variables de estructura como Internet y disponibilidad de salas con distintas finalidades parecen no impactar significativamente en la utilización de estas tecnologías, señalando que los factores como el apoyo institucional y la propia implantación de la telesalud en la unidad son más importantes para aumentar la adhesión al programa.

Machine learning analysis to predict health outcomes among emergency department users in Southern Brazil: a protocol study.

OBJECTIVE: Emergency services are essential to the organization of the health care system. Nevertheless, they face different operational difficulties, including overcrowded services, largely explained by their inappropriate use and the repeated visits from users. Although a known situation, information on the theme is scarce in Brazil, particularly regarding longitudinal user monitoring. Thus, this project aims to evaluate the predictive performance of different machine learning algorithms to estimate the inappropriate and repeated use of emergency services and mortality. METHODS: To that end, a study will be conducted in the municipality of Pelotas, Rio Grande do Sul, with around five thousand users of the municipal emergency department. RESULTS: If the study is successful, we will provide an algorithm that could be used in clinical practice to assist health professionals in decision-making within hospitals. Different knowledge dissemination strategies will be used to increase the capacity of the study to produce innovations for the organization of the health system and services. CONCLUSION: A high performance predictive model may be able to help decisionmaking in the emergency services, improving quality of care.

Multidrug-Resistant (MDR) Klebsiella variicola Strains Isolated in a Brazilian Hospital Belong to New Clones.

Klebsiella variicola is mainly associated with opportunistic infections and frequently identified as Klebsiella pneumoniae. This misidentification implies a wrong epidemiology result as well as incorrect attribution to K. pneumoniae as the etiology of some severe infections. Recently, huge efforts have been made to study K. variicola, however, the biological aspects of this species are still unclear. Here we characterized five K. variicola strains initially identified as K. pneumoniae, with a Vitek-2 System and 16S rRNA sequencing. One-step multiplex polymerase chain reaction and Whole Genome Sequencing (WGS) identified them as K. variicola. Additionally, WGS analysis showed that all the strains are closely related with K. variicola genomes, forming a clustered group, apart from K. pneumoniae and K. quasipneumoniae. Multilocus sequence typing analysis showed four different sequence types (STs) among the strains and for two of them (Kv97 and Kv104) the same ST was assigned. All strains were multidrug-resistant (MDR) and three showed virulence phenotypes including invasion capacity to epithelial cells, and survival in human blood and serum. These results showed the emergence of new K. variicola clones with pathogenic potential to colonize and cause infection in different tissues. These characteristics associated with MDR strains raise great concern for human health.

Effect of Early Treatment With Hydroxychloroquine or Lopinavir and Ritonavir on Risk of Hospitalization Among Patients With COVID-19: The TOGETHER Randomized Clinical Trial.

Importance: Data on the efficacy of hydroxychloroquine or lopinavir-ritonavir for the treatment of high-risk outpatients with COVID-19 in developing countries are needed. Objective: To determine whether hydroxychloroquine or lopinavir-ritonavir reduces hospitalization among high-risk patients with early symptomatic COVID-19 in an outpatient setting. Design, Setting, and Participants: This randomized clinical trial was conducted in Brazil. Recently symptomatic adults diagnosed with respiratory symptoms from SARS-CoV-2 infection were enrolled between June 2 and September 30, 2020. The planned sample size was 1476 patients, with interim analyses planned after 500 patients were enrolled. The trial was stopped after the interim analysis for futility with a sample size of 685 patients. Statistical analysis was performed in December 2020. Interventions: Patients were randomly assigned to hydroxychloroquine (800 mg loading dose, then 400 mg daily for 9 days), lopinavir-ritonavir (loading dose of 800 mg and 200 mg, respectively, every 12 hours followed by 400 mg and 100 mg, respectively, every 12 hours for the next 9 days), or placebo. Main Outcomes and Measures: The primary outcomes were COVID-19-associated hospitalization and death assessed at 90 days after randomization. COVID-19-associated hospitalization was analyzed with a Cox proportional hazards model. The trial included the following secondary outcomes: all-cause hospitalization, viral clearance, symptom resolution, and adverse events. Results: Of 685 participants, 632 (92.3%) self-identified as mixed-race, 377 (55.0%) were women, and the median (range) age was 53 (18-94) years. A total of 214 participants were randomized to hydroxychloroquine; 244, lopinavir-ritonavir; and 227, placebo. At first interim analysis, the data safety monitoring board recommended stopping enrollment of both hydroxychloroquine and lopinavir-ritonavir groups because of futility. The proportion of patients hospitalized for COVID-19 was 3.7% (8 participants) in the hydroxychloroquine group, 5.7% (14 participants) in the lopinavir-ritonavir group, and 4.8% (11 participants) in the placebo group. We found no significant differences between interventions for COVID-19-associated hospitalization (hydroxychloroquine: hazard ratio [HR], 0.76 [95% CI, 0.30-1.88]; lopinavir-ritonavir: HR, 1.16 [95% CI, 0.53-2.56] as well as for the secondary outcome of viral clearance through day 14 (hydroxychloroquine: odds ratio [OR], 0.91 [95% CI, 0.82-1.02]; lopinavir-ritonavir: OR, 1.04 [95% CI, 0.94-1.16]). At the end of the trial, there were 3 fatalities recorded, 1 in the placebo group and 2 in the lopinavir-ritonavir intervention group. Conclusions and Relevance: In this randomized clinical trial, neither hydroxychloroquine nor lopinavir-ritonavir showed any significant benefit for decreasing COVID-19-associated hospitalization or other secondary clinical outcomes. This trial suggests that expedient clinical trials can be implemented in low-income settings even during the COVID-19 pandemic. Trial Registration: ClinicalTrials.gov Identifier: NCT04403100.

Machine learning analysis to predict health outcomes among emergency department users in Southern Brazil: a protocol study / Análise de aprendizado de máquina para prever resultados de saúde entre usuários de serviços de emergência no sul do Brasil: estudo de protocolo

ABSTRACT: Objective: Emergency services are essential to the organization of the health care system. Nevertheless, they face different operational difficulties, including overcrowded services, largely explained by their inappropriate use and the repeated visits from users. Although a known situation, information on the theme is scarce in Brazil, particularly regarding longitudinal user monitoring. Thus, this project aims to evaluate the predictive performance of different machine learning algorithms to estimate the inappropriate and repeated use of emergency services and mortality. Methods: To that end, a study will be conducted in the municipality of Pelotas, Rio Grande do Sul, with around five thousand users of the municipal emergency department. Results: If the study is successful, we will provide an algorithm that could be used in clinical practice to assist health professionals in decision-making within hospitals. Different knowledge dissemination strategies will be used to increase the capacity of the study to produce innovations for the organization of the health system and services. Conclusion: A high performance predictive model may be able to help decisionmaking in the emergency services, improving quality of care.

RESUMO: Objetivo: Os serviços de emergência são fundamentais na organização da rede de atenção à saúde. Não obstante, apresentam diferentes dificuldades para seu funcionamento. Entre essas, destaca-se a superlotação dos serviços, a qual, em boa medida, é explicada pelo uso inadequado do serviço e reutilização frequente por parte de usuários. Apesar do conhecimento dessa situação, as informações sobre a temática são escassas no Brasil, ainda mais as relacionadas ao acompanhamento longitudinal dos usuários. Assim, este projeto objetiva avaliar a performance preditiva de diferentes algoritmos de machine learning para estimar o uso inapropriado e a reutilização dos serviços de emergência e a mortalidade. Métodos: Para isso, será realizado um estudo no município de Pelotas, Rio Grande do Sul, com um pouco mais de cinco mil usuários do pronto socorro municipal. Resultados: Caso o estudo seja bem-sucedido, será disponibilizado um algoritmo com potencial para ser usado na prática clínica para auxiliar profissionais de saúde na tomada de decisão no contexto hospitalar. Diferentes estratégias de difusão dos conhecimentos serão utilizadas para aumentar a capacidade do estudo de produzir inovações para a organização do sistema e serviços de saúde. Conclusão: Um modelo preditivo de alto desempenho pode auxiliar na tomada de decisão nos serviços de emergência, melhorando a qualidade do atendimento.

Intranasal administration of budesonide-loaded nanocapsule microagglomerates as an innovative strategy for asthma treatment.

The co-existence with rhinitis limits the control of asthma. Compared with oral H1 receptor antagonists, intranasal corticosteroids have been demonstrated to provide greater relief of all symptoms of rhinitis and are recommended as first-line treatment for allergic rhinitis. Intrinsic limitations of nasal delivery, such as the presence of the protective mucous layer, the relentless mucociliary clearance, and the consequent reduced residence time of the formulation in the nasal cavity, limit budesonide efficacy to the treatment of local nasal symptoms. To overcome these limitations and to enable the treatment of asthma via nasal administration, we developed a budesonide-loaded lipid-core nanocapsule (BudNC) microagglomerate powder by spray-drying using a one-step innovative approach. BudNC was obtained, as a white powder, using L-leucine as adjuvant with 75 ± 6% yield. The powder showed a bimodal size distribution curve by laser diffraction with a principal peak just above 3 µm and a second one around 0.45 µm and a drug content determined by HPLC of 8.7 mg of budesonide per gram. In vivo after nasal administration, BudNC showed an improved efficacy in terms of reduction of immune cell influx; production of eotaxin-1, the main inflammatory chemokine; and arrest of airways remodeling when compared with a commercial budesonide product in both short- and long-term asthma models. In addition, data showed that the results in the long-term asthma model were more compelling than the results obtained in the short-term model. Graphical abstract.