Unraveling the dynamic interplay of microbial communities associated to Lupinus angustifolius in response to environmental and cultivation conditions.

Microorganisms form dynamic communities with plants, providing benefits such as nutrient acquisition and stress resilience. Understanding how these microorganisms are affected by environmental factors such as growth conditions and soil characteristics are essential for harnessing these communities for sustainable agriculture practices and their response to climate change. The microbiome associated to Lupinus angustifolius, a legume native in Europe, with a high protein value and stress resilience was characterized for the first time. Using 16S rRNA gene and ITS amplicon sequencing, we characterized the compositional and temporal changes of the bacterial and fungal communities associated to the soil, rhizosphere, and plant compartments where Lupinus angustifolius grows naturally. Our results suggest that the main difference in the soil microbial communities is related to the edaphic properties, although environmental factors such as temperature, humidity or rainfall also influenced the composition of the soil microbial communities. We also characterized the bacterial communities associated with the rhizosphere, roots, nodules, and leaves of wild plants collected in the field and compared them against plants obtained under greenhouse conditions. In the plant compartments, the bacterial composition appeared to be more affected by the growing conditions (field vs greenhouse), than by soil characteristics or location. These results can be used to identify key taxa that may play crucial roles in the development and adaptation of the host plant and its associated microbiota to environmental changes and highlight the importance of characterizing the plant microbiomes in their natural habitats. Soil, influenced by climatic seasons, shapes the plant microbiome assembly. Lupinus recruits a core microbiome across rhizosphere, roots, nodules, and leaves, that is stable across locations. However, cultivation conditions may alter microbiome dynamics, impacting the adaptability of its components. Wild plants show a resilient and adaptable microbiome while germination and cultivation in greenhouse conditions alter its composition and vulnerability.

Prevalencia de depresión durante la gestación / Prevalence of depression during pregnancy

Objetivos: Estimar la prevalencia de depresión durante la gestación y estudiar posibles factores asociados a ésta. Material y Métodos: Entre los meses de marzo y julio del 2004, se incluyeron 203 pacientes embarazadas que acudían al Consultorio Externo de Obstetricia del Hospital Nacional Cayetano Heredia en Lima, Perú. Se registraron los datos personales, obstétricos, socioeconómicos y psiquiátricos de las gestantes, y se les aplicó la Escala de Depresión Postnatal de Edimburgo (EPDS). Resultados: Un 34,7% de las embarazadas tuvo puntajes de la EPDS > 13,5. La prevalencia estimada de depresión mayor en las pacientes gestantes, tomando en cuenta la sensibilidad y especificidad de la EPDS para el punto de corte 13,5, fue 22,25%. Se obtuvieron puntajes totales de EPDS con una media de 11,3 y una desviación estándar de 6,2. Los factores asociados a mayores niveles de depresión medidos con la EPDS fueron: ser soltera (p<0,001), ausencia de educación superior (p=0,039), embarazo no deseado por la pareja (p=0,004) o por la paciente (p=0,021) e historia de depresión previa a la gestación (p=0,003). Conclusión: La prevalencia estimada de depresión durante la gestación en las pacientes ambulatorias de nuestro estudio es más elevada que la encontrada en la literatura mundial en mujeres embarazadas y postparto, sin embargo, similar a la hallada en otro estudio realizado en el Perú con gestantes. Los factores asociados con mayores puntajes en la EPDS fueron ser soltera, no tener educación superior, no desear la gestación y haber presentado diagnóstico previo de depresión.

Objective: To estimate the prevalence of depression during pregnancy and to study the factors probably associated with it. Methods: We interviewed 203 pregnant women who went to Hospital Nacional Cayetano Heredia ObstetricsÆ Clinical Office in Lima, Perú, between March and July of 2004. We asked for personal, obstetric, socioeconomic and psychiatric factors; and applied the Edinburgh Postnatal Depression Scale (EPDS). Results: EPDS scores were greater than 13.5 in 34.7% of the subjects. The estimated prevalence of major depression, calculated taking into account the sensitivity and specificity of EPDS for a threshold of 13.5, was 22.25%. The average score of EPDS was 11.3, and the standard deviation 6.2. We found association between depression levels and single marital status (p<0.001), absence of superior education (p=0.039), absence of partnerÆs desire for pregnancy (p=0.004), absence of patientÆs desire for pregnancy (p=0.021) and history of previous depression (p=0.003). Conclusion: The estimated prevalence of depression during pregnancy in our outpatients was higher than that found in the literature in pregnant or postpartum women, however was similar to that found in another Peruvian study in pregnant women. The factors associated with having higher scores of EPDS were single marital status, absence of superior education, unwanted pregnancy and history of depression.

Urticaria pigmentosa: a propósito de un caso / Urticaria pigmentosa: Apropos of a case

Se presentó el caso de un paciente masculino, con manchas hiperpigmentadas en la piel con un tiempo de evolución de un año y prurito discreto. Se constataron lesiones máculo papulosas generalizadas respetando palmas, plantas y mucosas, con signo de Darier positivo. El estudio histopatológico de piel fue compatible con una Mastocitosis indolente, variedad Urticaria pigmentosa. La respuesta al tratamiento aplicado, fue satisfactoria, el paciente se encuentra estable, sin progresión de la enfermedad...(AU)

Al rescate del extractor de comedón en la microcirugía de acné: Colón 2003 / To recover the stractor of comedon in the microsurgery of acne: Colón 2003

El acné es una dermatosis crónica autolimitada que afecta el folículo pilosebáceo, representa la enfermedad cutánea más frecuente en los adolescentes, ésta no reacciona de manera máxima a un grupo aislado de fármacos y se requiere de la combinación de medicamentos tópicos, sistémicos y la microcirugía para complementar el tratamiento. Decidimos rescatar el instrumento extractor de comedón para el drenaje de las lesiones cutáneas elaborándolo a partir de instrumentos desechados en la clínica dental, con un material accesible de bajo costo y lo más semejante posible al importado con la posibilidad de la generalización en el país...(AU)