RÉSUMÉ
Excitatory postsynaptic currents (EPSC) were recorded from pyramidal neurons of the rat hippocampal slices as well as the AMPA and NMDA components. A strong enough correlation between the amplitudes of the components provided a reliable evidence of their generation by the same synapse. Both components revealed similar LTP following afferent tetanisation. The data obtained do not support postsynaptic mechanisms of the LTP maintenance but suggest that increased presynaptic release represents a basic mechanism of the early LTP maintenance.
Sujet(s)
Hippocampe/physiologie , Potentialisation à long terme/physiologie , Récepteur de l'AMPA/physiologie , Récepteurs du N-méthyl-D-aspartate/physiologie , Synapses/physiologie , Animaux , Stimulation électrique , Antagonistes des acides aminés excitateurs/pharmacologie , Techniques in vitro , Techniques de patch-clamp , Rats , Rat Wistar , Récepteur de l'AMPA/antagonistes et inhibiteurs , Récepteurs du N-méthyl-D-aspartate/antagonistes et inhibiteursRÉSUMÉ
Application of picrotoxin to apical dendrites in the CA1 area of the rat hippocampal slices increased the duration of focal potentials (FP) in the stratum radiatum but did not affect the unit activity spikes' amplitude in the stratum pyramidalis, the finding suggesting a suppression of the feed-forward inhibition with no effect upon the recurrent one. The data obtained shows that, under conditions normally used for studying the NMDA-EPSP feed-forward, the inhibition is not invariably suppressed and may affect the results of the studies.