RÉSUMÉ
We present the MOlecular NETwork (MONET) ontology as a model to integrate data from different networks that govern cell function. To achieve this, different existing ontologies were analyzed and an integrated ontology was built in a way to make it possible to share and reuse knowledge, support interoperability between systems, and also allow the formulation of hypotheses through inferences. By studying the cell as an entity of a myriad of elements and networks of interactions, we aim to offer a means to understand the large-scale characteristics responsible for the behavior of the cell and to enable new biological insights.
Sujet(s)
Humains , Animaux , Algorithmes , Phénomènes physiologiques cellulaires , Modèles biologiques , Bases de données comme sujet , Analyse sur microréseau/méthodes , Simulation numériqueRÉSUMÉ
Extracellular recording techniques were used to study the effects of the nitric oxide releasing agents diethylamine-NO (DEA-NO) and S-nitroso-N-acetyl-penicillamine (SNAP) on synaptic transmission in the intermediate and medial part of the hyperstriatum ventrale (IMHV), a part of the domestic chick forebrain that is essential for some forms of early learning. The field response evoked by local electrical stimulation was recorded in the IMHV in an in vitro slice preparation. DEA-NO (100-200 mgr) significantly depressed the field response in a concentration dependent and reversible manner. However, the depression produced by perfusion with 400 mgr DEA-NO, was not reversed following washout of the drug. With 400 mgr DEA-NO, NO reaches a maximum concentration of 10 mgr at 2 min of perfusion, and then declines slowly. SNAP (400 mgr) produced an effect similar to 400 mgr DEA-NO. Neither the immediate nor the longer-term depressive effect of NO is mediated by activation of guanylyl cyclase because in the presence of both low and high doses of ODQ, a potent and selective inhibitor of NO-stimulated guanylyl cyclase, NO produced the same depression of the field response. There is evidence however that the IMHV possesses c-GMP responsive elements since direct perfusion of 8-Br-cGMP (1 mM) produced a long-term but not an immediate depression. The long-term depression produced by 400 mgr DEA-NO was eliminated in the presence of either a selective adenosine A(1) receptor antagonist or an ADP-ribosyltransferase inhibitor. It was also possible to prevent the long-term effect in the presence of tetraethyl ammonium a K(+)-channel blocker. These results suggest that the NO may be acting presynaptically in a synergistic fashion with the adenosine A(1) receptor to depress transmitter release.
Sujet(s)
Conditionnement psychologique/physiologie , Mémoire/physiologie , Monoxyde d'azote/métabolisme , Prosencéphale/croissance et développement , Prosencéphale/métabolisme , Animaux , Poulets , GMP cyclique/analogues et dérivés , GMP cyclique/pharmacologie , Potentiels évoqués/physiologie , Guanylate cyclase/métabolisme , Hydrazines/pharmacologie , Plasticité neuronale/physiologie , Neurones/effets des médicaments et des substances chimiques , Neurones/enzymologie , Donneur d'oxyde nitrique/pharmacologie , Oxydes d'azote , Pénicillamine/analogues et dérivés , Pénicillamine/pharmacologie , Poly(ADP-ribose) polymerases/métabolisme , Canaux potassiques/physiologie , Récepteurs alpha-1 adrénergiques/physiologie , Synapses/physiologie , Transmission synaptique/effets des médicaments et des substances chimiques , Transmission synaptique/physiologie , Tétraéthyl-ammonium/pharmacologie , Xanthines/pharmacologieRÉSUMÉ
1. The effect of several central nervous system active drugs was studied in vitro on ATPase-ADPase activity and acetylcholinesterase (AChE) activity from the cerebral cortex of adult rats. 2. Lithium (1.0-10.0 mM) had no effect on either ATPase-ADPase or acetylcholinesterase activity. 3. Imipramine (0.5-5.0 mM), desipramine (0.5-5.0 mM), amitriptyline (0.1-1.0 mM) and diazepam (0.5-2.0 mM) inhibited ATP and ADP hydrolysis at all concentrations tested. 4. AChE activity was altered by imipramine (1.0-2.0 mM) and by diazepam (0.5-2.0 mM). 5. The possible participation of ATP diphosphohydrolase and AChE in the action of these drugs cannot be ruled out. The probable reduction of ATP, ADP and acetylcholine hydrolysis by the inhibitory effect of these drugs is discussed.
Sujet(s)
Acetylcholinesterase/métabolisme , Adenosine triphosphatases/métabolisme , Apyrase/métabolisme , Agents du système nerveux central/pharmacologie , Cortex cérébral/effets des médicaments et des substances chimiques , Cortex cérébral/enzymologie , Acetylcholinesterase/analyse , Amitriptyline/pharmacologie , Animaux , Apyrase/analyse , Désipramine/pharmacologie , Diazépam/pharmacologie , Hydrolases/analyse , Hydrolases/métabolisme , Imipramine/pharmacologie , Techniques in vitro , Lithium/pharmacologie , Rats , Synaptosomes/métabolismeRÉSUMÉ
Aluminum chloride (AlCl3), a neurotoxic compound, inhibited ATP diphosphohydrolase activity of synaptosomes obtained from cerebral cortex of adult rats. The metal ion significantly inhibited ATPase and ADPase activities of the enzyme at all concentrations tested in vitro (0.01, 0.05, 0.5, 5, and 10 mM) in the presence of 1.5 mM calcium. When tested in the absence of Ca2+, and with increasing amounts of Al3+, enzyme activity remained below basal levels, suggesting that the trivalent cation Al3+ is not a substitute for the divalent cation Ca2+ in ATP-Ca2+ and ADP-Ca2+ complexes. The Al3+ inhibition was competitive with respect to Ca2+. The enzyme inhibition was reversed by the addition of deferoxamine (DFO). NaF significantly inhibited ATP diphosphohydrolase activity, and this inhibition was reversed by the addition of Ca2+ to the medium. Such inhibition was not potentiated by AlF4, which is an inhibitor of cation-transport ATPases.
Sujet(s)
Composés de l'aluminium/toxicité , Apyrase/métabolisme , Astringents/toxicité , Cortex cérébral/effets des médicaments et des substances chimiques , Chlorures/toxicité , Synaptosomes/enzymologie , Adenosine triphosphatases/antagonistes et inhibiteurs , Chlorure d'aluminium , Animaux , Apyrase/antagonistes et inhibiteurs , Calcium/métabolisme , Cortex cérébral/enzymologie , Chélateurs/pharmacologie , Déferoxamine/pharmacologie , Interactions médicamenteuses , Synergie des médicaments , Antienzymes/toxicité , L-Lactate dehydrogenase/métabolisme , Mâle , Protéines/métabolisme , Rats , Rat Wistar , Fluorure de sodium/toxicité , Transmission synaptique/effets des médicaments et des substances chimiques , Synaptosomes/effets des médicaments et des substances chimiquesRÉSUMÉ
ATP diphosphohydrolase (EC 3.6.1.5; apyrase) is an enzyme that can promote ATP and ADP hydrolysis to AMP plus inorganic phosphate and depends on divalent cations such as Ca2+ or Mg2+. In previous papers we described this enzyme in the synaptosomal fraction from the central and peripheral nervous system. The present report examines whether cadmium acetate could affect the in vitro activity of the enzyme in the synaptosomal fraction from the cerebral cortex of adult male Wistar rats. Cadmium (Cd2+), a heavy metal with neurotoxic effects, inhibited the enzyme in a concentration-dependent manner. All concentrations tested (0.05-1.0 mM) significantly inhibited the hydrolysis of both substrates (ATP and ADP), with the exception of 0.05 mM on ATP hydrolysis. The kinetic data indicate a noncompetitive inhibition between the cations Cd2+ and Ca2+.
Sujet(s)
Acétates , Apyrase/antagonistes et inhibiteurs , Cadmium/pharmacologie , Cortex cérébral/enzymologie , Synaptosomes/enzymologie , ADP/métabolisme , Adenosine triphosphatases/métabolisme , Adénosine triphosphate/métabolisme , Animaux , Apyrase/métabolisme , Hydrolyse , Cinétique , Mâle , Rats , Rat Wistar , Spécificité du substratRÉSUMÉ
Cerebral ischemia causes cell death of vulnerable neurons in mammalian brain. Wistar adult rats (male and female, weighing 180-280 g) were submitted to 2 min, 10 min, or to 2 and 10 min (separated by a 24-h interval) of transient forebrain ischemia by the four-vessel occlusion method. Animals subjected to the longer ischemic episodes had massive necrosis of pyramidal CA1 cells of the hippocampus, while animals receiving double ischemia (2 + 10 min) showed neuronal tolerance to the ischemic insult. ATP-diphosphohydrolase activity from hippocampal synaptosomes was assayed in these three groups (N = 6 animals/group) under two conditions: no reperfusion and 5-min of reperfusion. The control values for ATPase and ADPase activities were 144.7 +/- 18.8 and 60.6 +/- 5.24 nmol Pi min-1 mg protein-1, respectively. The 10-min group without reperfusion showed an enhancement of approximately 20% for ATPase and ADPase activities. In reperfused rats, only the 2-min group had a 20% increase in both enzymatic activities. We suggest that modulation of ATP-diphosphohydrolase activity might be involved in molecular events that follow both ischemia and reperfusion.
Sujet(s)
Apyrase/métabolisme , Hippocampe/enzymologie , Accident ischémique transitoire/enzymologie , Synaptosomes/enzymologie , Adenosine triphosphatases/métabolisme , Animaux , Femelle , Mâle , Rats , Rat Wistar , Reperfusion , Facteurs tempsRÉSUMÉ
Cerebral ischemia causes cell death of vulnerable neurons in mammalian brain. Wistar adult rats (male and female, weighing 180-280 g) were submitted to 2 min, 10 min, or to 2 and 10 min (separated by a 24-h interval) of transient forebrain ischemia by the four-vessel occlusion method. Animals subjected to the longer ischemic episodes had massive necrosis of pyramidal CA1 cells of the hippocampus, while animals receiving double ischemia (2 + 10 min) showed neuronal tolerance to the ischemic insult. ATP-diphosphohydrolase activity from hippocampal synaptosomes was assayed in these three groups (N = 6 animals/group) under two conditions: no reperfusion and 5-min of reperfusion. The control values for ATPase and ADPase activities were 144.7 +/- 18.8 and 60.6 +/- 5.24 nmol Pi min-1 mg protein-1, respectively. The 10-min group without reperfusion showed an enhancement of approximately 20 for ATPase and ADPase activities. In reperfused rats, only the 2-min group had a 20 increase in both enzymatic activities. We suggest that modulation of ATP-diphosphohydrolase activity might be involved in molecular events that follow both ischemia and reperfusion.
Sujet(s)
Animaux , Mâle , Femelle , Rats , Apyrase , Accident ischémique transitoire/enzymologie , Hippocampe , Synaptosomes , Adenosine triphosphatases , Rat Wistar , Reperfusion , Facteurs tempsRÉSUMÉ
ATP diphosphohydrolase (EC 3.6.1.5; apyrase) is an enzyme that can promote ATP and ADP hydrolysis to AMP plus inorganic phosphate and depends on divalent cations such as Ca2+ or Mg2+. In previous papers we described this enzyme in the synaptosomal fraction from the central and peripheral nervous system. The present report examines whether cadmium acetate could affect the in vitro activity of the enzyme in the synaptosomal fraction from the cerebral cortex of adult male Wistar rats. Cadmium (Cd2+), a heavy metal with neurotoxic effects, inhibited the enzyme in a concentration-dependent manner. All concentrations tested (0.05-1.0 mM) significantly inhibited the hydrolysis of both substrates (ATP and ADP), with the exception of 0.05 mM on ATP hydrolysis. The kinetic data indicate a noncompetitive inhibition between the cations Cd2+ and Ca2+.
Sujet(s)
Animaux , Mâle , Rats , Apyrase , Cadmium , Cortex cérébral/enzymologie , Synaptosomes , ADP , Adenosine triphosphatases , Adénosine triphosphate , Apyrase , Hydrolyse , Cinétique , Rat Wistar , Spécificité du substratRÉSUMÉ
Data from the literature have demonstrated that synaptosomal preparations from various sources can hydrolyze externally added ATP. Various authors characterized this activity as an ecto-ATPase. In the present report, we demonstrate that synaptosomal preparations obtained from the cerebral cortex of rats show ATPase activity that could not be dissociated from ADPase activity, suggesting that an ATP-diphosphohydrolase is involved in ATP and ADP hydrolysis. Furthermore, the ATP and ADP hydrolysis could not be attributed to associations of enzymes that could mimic an ATP-diphosphohydrolase because none of the following activities were detected in our assay conditions inorganic pyrophosphatase, adenylate kinase, or nonspecific phosphatases. A possible association between an ATPase and an ADPase was excluded on the basis of both the kinetics and much additional data on inhibitors, ion dependence, pH, etc. The present results demonstrate that in synaptosomal preparations from cerebral cortex an ATP-diphosphohydrolase is involved, at least in part, in ATP and ADP hydrolysis.