RÉSUMÉ
A randomized prospective study of 191 patients with concomitant brain injury (CBI) of different severity has been carried out. All patients underwent surgery and received treatment in reanimation and intensive care departments. The main group consisted of 100 (52.4%) patients treated with cytoflavin in dosage 20-40 ml daily intravenously in drops during 10 days in addition to standard treatment. The comparison group included 91 (47.6%) patients who received standard treatment only. A positive effect of cytoflavin on clinical symptoms and laboratory characteristics of patients with CBI was identified. The decrease in severity measured with the APACHE II, higher activation of consciousness and improvement in the dynamics of neurological symptoms in these patients have resulted in the reduction in the duration of artificial lung ventilation and total hospital stay.
Sujet(s)
Lésions encéphaliques/thérapie , Soins de réanimation/méthodes , Métabolisme énergétique/physiologie , Flavine mononucléotide/usage thérapeutique , Homéostasie , IDP/usage thérapeutique , Nicotinamide/usage thérapeutique , Ventilation artificielle/méthodes , Succinates/usage thérapeutique , Adolescent , Adulte , Lésions encéphaliques/métabolisme , Association médicamenteuse , Femelle , Mortalité hospitalière/tendances , Humains , Durée du séjour/tendances , Mâle , Adulte d'âge moyen , Études prospectives , Résultat thérapeutique , Jeune adulteRÉSUMÉ
Complete genome sequencing was performed for Anabaena variabilis ATCC 29413 from the collection of the Chair of Genetics, Department of Biology, Moscow State University, Russia. In addition to known plasmids A, B, and C, a new circular low-copy plasmid was detected and named D. It was also sequenced completely and found to have 27051 bp. The plasmid contained the parA and parB genes of the partition system, two genes that encode replication proteins, a gene for site-specific recombinase, atype-I restriction-modification system, and several genes with unknown functions. Analysis by PCR revealed the presence of plasmid D in two epiphytic strains from Vietnam, i.e., Anabaena sp. 182 and Anabaena sp. 281, as well as in Anabaena sp. V5 and A. azollae (Newton's isolate).
Sujet(s)
Anabaena variabilis/génétique , Plasmides/génétique , Anabaena variabilis/isolement et purification , DNA nucleotidyltransferases , Gènes bactériens , Analyse de séquence d'ADN , VietnamSujet(s)
Archéobactéries/classification , Cyanobactéries/classification , Sources thermales/microbiologie , Archéobactéries/génétique , Archéobactéries/isolement et purification , Cyanobactéries/génétique , Cyanobactéries/isolement et purification , ADN ribosomique/génétique , ARN ribosomique 16S/génétique , Russie , Analyse de séquence d'ADN , Soufre/métabolismeRÉSUMÉ
AIM: To evaluate expression patterns of protein product of putative tumor suppressor gene TSC-22 in human astrocytic tumors by immunohistochemical approach. METHODS: Plasmid pET-23d-TSC22 was constructed for the expression of human TSC-22 protein in bacterial system, and polyclonal rabbit antibodies against recombinant TSC-22 were produced. Immunohistochemical analysis of TSC-22 and GFAP expression with the use of anti-human-TSC-22- and anti-human-GFAP-antibodies was performed on histological slides of astrocytic tumors. RESULTS: Immunohistochemical analysis has shown that the number of cells expressing TSC-22 was significantly lower in glioblastoma tissues than that in diffuse astrocytoma. Double immunohistochemical staining of astrocytic tumors using anti-human-TSC-2- and anti-human-GFAP-antibodies showed that both TSC-22 and GFAP expression is co-localized in astrocytes. CONCLUSION: TSC-22 protein is expressed in astrocytes, but not in macrophage/microglial cells. In more aggressive forms of astrocytic tumors decreased expression of TSC-22 mRNA correlates with its lowered expression on protein level.
Sujet(s)
Astrocytome/métabolisme , Tumeurs du cerveau/métabolisme , Régulation de l'expression des gènes tumoraux , Protéines de répression/biosynthèse , Séquence d'acides aminés , Astrocytes/métabolisme , Astrocytome/anatomopathologie , Séquence nucléotidique , Marqueurs biologiques tumoraux/analyse , Tumeurs du cerveau/anatomopathologie , Analyse de profil d'expression de gènes , Protéine gliofibrillaire acide/biosynthèse , Humains , Immunohistochimie , Microglie/métabolisme , Données de séquences moléculaires , Protéines recombinantes/biosynthèse , Protéines recombinantes/génétique , Protéines de répression/génétiqueRÉSUMÉ
Limbic-reticular dysfunction due to pathologic rearrangement in functional intracentral relations was evidenced in 85 syringomyelic patients. The dysfunction was initiated by the syringomyelic inflicted focus within the brainstem (syringobulbia) or spinal cord. Displayed were the psychoemotional, unspecific activational and hemodynamic correlates of this pathophysiological phenomenon. This is the first description of the radioactive iodine positive effect on limbic-reticular dysfunction in syringomyelia.