RÉSUMÉ
Wastewater containing tetrabromobisphenol A (TBBPA), a commonly used flame retardant found in wastewater, can present significant toxic effects on biota, yet its impact on tropical freshwater environments is not well understood. This study explores the effectiveness of two independent anaerobic treatment systems, the acidogenic reactor (AR) and the methanogenic reactor (MR), for the ecotoxicity reduction of TBBPA-rich wastewater in four tropical freshwater species. Despite presenting good physicochemical performance and reduced toxicity of the influent for most species, AR and MR treatments remain acute and chronic toxicity. Overall, MR exhibited greater efficacy in reducing influent toxicity compared with AR. TBBPA bioaccumulation was observed in Chironomus sancticaroli after short-term exposure to 100% MR effluent. Multigenerational exposures highlighted changes in the wing length of C. sancticaroli, showing decreases after influent and AR exposures and increases after MR exposures. These findings underscore the need for ecotoxicological tools in studies of new treatment technologies, combining the removal of emerging contaminants with safeguarding aquatic biota. PRACTITIONER POINTS: Acidogenic and methanogenic reactors reduced the acute and chronic toxicity of wastewater containing tetrabromobisphenol A. Both treatments still exhibit toxicity, inducing short- and long-term toxic effects on four native tropical species. The aquatic species Pristina longiseta was most sensitive to effluents from acidogenic and methanogenic reactors. TBBPA concentrations recovered from Chironomus sancticaroli bioaccumulation analysis ranged from 1.07 to 1.35 µg g-1. Evaluating new treatment technologies with multiple species bioassays is essential for a comprehensive effluent toxicity assessment and ensuring aquatic safety.
Sujet(s)
Polybromobiphényles , Polluants chimiques de l'eau , Animaux , Polybromobiphényles/toxicité , Polybromobiphényles/métabolisme , Polluants chimiques de l'eau/toxicité , Polluants chimiques de l'eau/métabolisme , Anaérobiose , Eaux usées/composition chimique , Biote , Ignifuges/toxicité , Ignifuges/métabolisme , Élimination des déchets liquides/méthodes , Chironomidae/effets des médicaments et des substances chimiques , Chironomidae/métabolisme , Organismes aquatiques/effets des médicaments et des substances chimiques , Organismes aquatiques/métabolismeRÉSUMÉ
Aquatic worms are considered a suitable group to evaluate the effects of contaminants on the environment, although one of the main challenges is to use the species of local occurrence. Recently, Pristina longiseta was suggested to be used in acute bioassays. In this context, this study aimed to establish a chronic exposure for ecotoxicological bioassays using the cosmopolitan species of occurrence in Brazilian freshwater P. longiseta. Firstly, we tested three exposure times (4, 7, and 10 days) under the presence or absence of aeration for reproduction outputs. After determining the best configuration (7 days without aeration), we assessed the effects of the chronic exposures using the standardized reference substance potassium chloride (KCl), the antibiotic sulfamethoxazole (SMX), the flame retardant tetrabromobisphenol A (TBBPA), and the sugarcane vinasse. Our results showed suitability for applying the chronic exposure using P. longiseta and indicated the sensitivity of the offspring to KCl (EC50-7d = 0.51 g/L). Sulfamethoxazole and TBBPA caused a significant decrease in the offspring of P. longiseta (EC50-7d = 59.9 µg/L and < 62.5 µg/L, respectively). Sugarcane vinasse showed high toxicity for the species, and 4.26% of vinasse was calculated as EC50-7d. Therefore, the described protocol was successfully applied as an ecotoxicological bioassay to evaluate the effects of environmental contaminants on the reproduction rate of the freshwater worm P. longiseta.
Sujet(s)
Oligochaeta , Polybromobiphényles , Polluants chimiques de l'eau , Animaux , Polybromobiphényles/analyse , Dosage biologique , Reproduction , Polluants chimiques de l'eau/toxicitéRÉSUMÉ
Safe drinking water' supply is an essential service and depends directly on the water treatment that produces water treatment sludge (WTS) as a product, whose final destination varies and remains a challenge. The ecotoxicity assessment of the WTS address the ecological implications of the WTS disposal but these information is still scarce in the literature. In this sense, we did a systematic review of the ecotoxicological studies on WTS using databases from six platforms. From the 785 papers recovered; 16 studies were eligible and showed the ecotoxicity assays' applicability to evaluate the WTS. We discussed WTS ecotoxicity considering sample characterization; terrestrial and aquatic toxicity assays; and WTS challenges. WTS proved to be a highly heterogeneous matrix composed mainly of coagulant precipitates, including Al and Fe. Studies lack consensus concerning the most representative/sensitive species for evaluating WTS' toxicity. Crustaceans were the most studied aquatic group, although algae species were more sensitive. Besides, soil ecotoxicity assessed only plant growth, and a single study used the earthworm. Even papers used bioassays to indicate the recycling WTS' feasibility, there is a lack of specific legislation regarding the WTS reuse. Furthermore, are necessary a regulation for WTS management that involves an ecological risk assessment.
Sujet(s)
Oligochaeta , Polluants chimiques de l'eau , Purification de l'eau , Animaux , Biote , Écotoxicologie , Eaux d'égout , Polluants chimiques de l'eau/analyse , Polluants chimiques de l'eau/toxicitéRÉSUMÉ
The use of ecotoxicological bioassays has been increasing due to the importance of understanding the effects of substances on biota and to help environmental agencies determine water and sediment qualities. The use of Chironomus sp. in laboratory bioassays is extensive, but there is still a lack of studies regarding the application of extended ecotoxicological tests, which evaluate different population generation responses and show a detailed impact on their development. The present study investigated the response of Chironomus sancticaroli, a Brazilian endemic insect, to 17α-ethinylestradiol hormone, caffeine anhydrous, LAS (linear alkylbenzene sulfonate), and environmental samples (sediments) in relation to wing length, fecundity, and larval length over three generations. Statistically, differences for all contaminants between P (parental), F1, and F2 generations (p ≤ 0.05) were observed, indicating that in the environment, the organism may be negatively affected when exposed to contaminants in successive generations. Different ecological responses were also noted in comparison to classic acute (96h) and chronic (10 days) ecotoxicological tests using Chironomus sancticaroli. Our results highlight that the generation test can present more detailed results regarding the effects of stressors on the organism's life cycle than do the classic bioassays.
Sujet(s)
Chironomidae , Polluants chimiques de l'eau , Animaux , Brésil , Écotoxicologie , Eau douce , Sédiments géologiques , Polluants chimiques de l'eau/analyse , Polluants chimiques de l'eau/toxicitéRÉSUMÉ
An accurate and sensitive ultrasound-dispersive liquid-liquid microextraction technique followed by high-performance liquid chromatography separation coupled with electrospray ionization tandem mass spectrometry detection method to determine the presence of tetrabromobisphenol A (TBBPA) in complex environmental matrices is proposed. The miniaturized procedure was used to extract and quantify the analyte in domestic sewage, anaerobic sludge, and the aquatic test organism species Daphnia magna and Chironomus sancticaroli, which are standardized organisms for ecotoxicity bioassays. Limits of detection of 2 ng L-1 (domestic sewage), 2 ng g-1 (anaerobic sludge), 0.25 ng g-1 (D. magna), and 5 ng g-1 (C. tentans) were obtained. The presence of TBBPA was determined in domestic sewage and anaerobic sludge from an anaerobic batch bioreactor at a concentration of 0.2 ± 0.03 µg L-1 and 507 ± 79 ng g-1 , respectively. In D. magna and C. sancticaroli exposed to TBBPA in an acute toxicity bioassay, the micropollutant accumulated at 3.74 and 8.87 µg g-1 , respectively. The proposed method is a simple and cost-effective tool to determine TBBPA environmental occurrence and biomagnification potential compared with conventional extraction methods. To the best of our knowledge, this is the first liquid-liquid miniaturized extraction method to be applied to D. magna and C. sancticaroli. Environ Toxicol Chem 2020;39:2147-2157. © 2020 SETAC.