Non-linear association of atherogenic index of plasma with bone mineral density a cross-sectional study.

INTRODUCTION: Although there has been abundant evidence of the association between dyslipidemia as a single factor and osteoporosis, the non-linear relationship between osteoporosis and the Atherogenic Index of Plasma (AIP) has not yet been thoroughly investigated. This study aimed to investigate the complex relationship between AIP and bone mineral density (BMD) to elucidate their interrelationship. METHODS: An analysis of 2007-2018 National Health and Nutrition Survey (NHANES) data was conducted for this study. The study enrolled 5,019 participants. Logarithmically multiplying triglycerides and high-density lipoprotein cholesterol yields the AIP (base 10). The measured variables consisted of BMD in the total femur (TF), femoral neck (FN), and lumbar spine (LS). The association between AIP and BMD was examined using a range of statistical models, such as weighted multivariable logistic regression, generalized additive model, etc. RESULTS: It was found that AIP was positively associated with BMD after adjusting for age, gender, race, socioeconomic status, degree of education, income, Consuming alcoholic beverages, osteoporosis status (Yes or No), ALT, AST, serum creatinine, and total calcium levels. Further studies supported the association link between elevated BMD and AIP. Furthermore, compared to men, females had a higher positive connection between AIP and BMD. In general, there was a curve in the reverse L-shape seen, with a point of change around 0.877, indicating a relationship between AIP and TF BMD. Moreover, a curve exhibiting an L-formed pattern, with a point of inflection at around 0.702, was seen between AIP and FN BMD. In addition, a J-shaped curve was seen, with a point of inflection at 0.092, which demonstrates the association between AIP and LS BMD. CONCLUSION: The AIP and TF BMD curves resemble inverted L shapes, as do the AIP and FN BMD curves. The relationship between AIP and LS BMD was further demonstrated by a J-shaped curve. The results indicate a possible association between AIP and bone mineral density, which should be explored in more detail.

Circ_0104652 Promotes the Proliferation and Migration of ox-LDL-Stimulated Vascular Smooth Muscle Cells via Stabilizing ADAMTS7 and HMGB1.

BACKGROUND: Atherosclerosis (AS) stands as the primary contributor to cardiovascular disease, a pervasive global health concern. Extensive research has underscored the pivotal role of circular RNAs (circRNAs) in cardiovascular disease development. However, the specific functions of numerous circRNAs in AS remain poorly understood. METHODS: Quantitative real-time PCR analysis revealed a significant upregulation of circ_0104652 in oxidized low-density lipoprotein (ox-LDL)-induced vascular smooth muscle cells (VSMCs). Loss-of-function experiments were subsequently employed to assess the impact of circ_0104652 on ox-LDL-induced VSMCs. RESULTS: Silencing circ_0104652 was found to impede the proliferation and migration while promoting the apoptosis of ox-LDL-stimulated VSMCs. Mechanistic assays unveiled that circ_0104652 stabilized ADAM metallopeptidase with thrombospondin type 1 motif 7 (ADAMTS7) and high mobility group box 1 (HMGB1) by recruiting eukaryotic translation initiation factor 4A3 (EIF4A3) protein. Rescue assays further confirmed that circ_0104652 exerted its influence on ox-LDL-induced VSMC proliferation through modulation of ADAMTS7 and HMGB1. CONCLUSIONS: This study elucidates the role of the circ_0104652/EIF4A3/ADAMTS7/HMGB1 axis in ox-LDL-stimulated VSMCs, providing valuable insights into the intricate mechanisms involved.

Bioinspired stormwater control measure for the enhanced removal of truly dissolved polycyclic aromatic hydrocarbons and heavy metals from urban runoff.

Stormwater harvesting (SWH) addresses the UN's Sustainable Development Goals (SDGs). Conventional stormwater control measures (SCMs) effectively remove particulate and colloidal contaminants from urban runoff; however, they fail to retain dissolved contaminants, particularly substances of concern like polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs), thereby hindering the SWH applicability. Here, inspired by protein folding in nature, we reported a novel biomimetic SCM for the efficient removal of dissolved PAHs and HMs from urban runoff. Lab-scale tests were conducted together with a more mechanistic investigation on how the contaminants were removed. By integrating hydrophobic organic chains with low-cost hydrophilic flocculant matrixes, our biomimetic flocculants achieved a 1.4-9.5 times removal of all detected dissolved PAHs and HMs, while enhancing the removal of a wide-spectrum of particulate and colloidal contaminants, compared to existing SCMs. Ecotoxicity, as indicated by newborn Daphnia magna as experimental organisms, was reduced from "acute toxicity" of the original runoff sample (toxic unit of ∼2.6) to "non-toxicity" (toxic unit < 0.4) of the treated water. The improved performance is attributed to the protein-folding-like features of the bioinspired flocculants providing: (i) stronger binding to PAHs (via hydrophobic association) and HMs (via coordination), and (ii) the ability of spontaneous aggregation. The bio-inspired approach in this work holds strong promise as an alternative or supplementary component in SCM systems, and is expected to contribute to sustainable water management practices in relation to SDGs.

Organic binding iron formation and its mitigation in cation exchange resin assisted anaerobic digestion of chemically enhanced primary sedimentation sludge.

Fe based chemically enhanced primary sedimentation (CEPS) is an effective method of capturing the colloidal particles and inorganic phosphorous (P) from wastewater but also produces Fe-CEPS sludge. Anaerobic digestion is recommended to treat the sludge for energy and phosphorus recovery. However, the aggregated sludge flocs caused by the coagulation limited sludge hydrolysis and P release during anaerobic digestion process. In this study, cation exchange resin (CER) was employed during anaerobic digestion of Fe-CEPS sludge with aims of prompting P release and carbon recovery. CER addition effectively dispersed the sludge flocs. However, the greater dispersion of sludge flocs could not translate to higher sludge hydrolysis. The maximum hydrolysis and acidification achieved at lower CER dosage of 0.5 g CER/g TS. It was observed that the extents of sludge hydrolysis and acidification had a strongly negative correlation with the organic binding iron (OBI) concentration. The presence of CER during anaerobic digestion favored Fe(III) reduction to Fe(II), and then further induced iron phase transformation, leading to the OBI formation from the released organic matters. Meanwhile, higher CER dosage resulted in higher P release efficiency and the maximum efficiency at 4 g CER/g TS was four times than that of the control. The reduction of BD-P, NaOH-P and HCl-P in solid phase contributed most P release into the supernatant. A new two-stage treatment process was further developed to immigrate the OBI formation and improve the carbon recovery efficiency. Through this process, approximately 45% of P was released, and 63% of carbon was recovered as methane from Fe-CEPS sludge via CER pretreatment.

Exploration and validation of the hub genes involved in hypoxia-induced endothelial-mesenchymal transition of systemic sclerosis.

OBJECTIVES: During the development of systemic sclerosis (SSc), endothelial-mesenchymal transition (EndoMT) has been shown to be one of the mechanisms leading to pulmonary fibrosis. However, the correlation between hypoxia and EndoMT was mostly unknown. METHODS: R software was used to analyse differentially expressed genes (DEGs) in vascular endothelial cells under hypoxic conditions, and fibroblasts derived from SSc-related pulmonary fibrotic tissues, respectively. Using a web-based online Venn diagram tool, we analysed overlapping genes of DEGs between endothelial cells and fibroblasts. Finally, the protein-protein interaction network of EndoMT hub genes were constructed using the STRING database. The hub genes were knockdown by transfection of siRNAs in the hypoxia model of HULEC-5a cells constructed by liquid paraffin closure and then used to detect the effect on EndoMT-related biomarkers by western blot. RESULTS: In this study, we found that INHBA, DUSP1, NOX4, PLOD2, BHLHE40 were upregulated in SSc fibroblasts and hypoxic-treated endothelial cells, while VCAM1, RND3, CCL2, and TXNIP were downregulated. In the hypoxia model of HULEC-5a cells, the expression of these 9 hub genes was confirmed by western blot. In addition, through Spearman's correlation analysis and Western blot, we confirmed that these hub genes were closely related to the EndoMT-related markers. The mechanisms of these hypoxia-induced EndoMT hub genes may be related to TGF-ß, Notch, Wnt, NF-κ B, TNF and mTOR signalling pathways. CONCLUSIONS: Our study provides new insights into the occurrence and development of SSc-related pulmonary fibrosis resulting from hypoxia-induced EndoMT.

Optimal emission reductions pathway for polybrominated diphenyl ethers in typical household e-waste dismantling products.

Waste printed circuit boards (WPCBs) and waste epoxy resin powder (WERP) generated after crushing are the most crucial hazardous materials in the recycling process of household e-waste. In this study, a sustainable treatment approach was established in response to the drawbacks of traditional treatment methods. The baseline and hypothetical scenarios were as follows: (1) scenario 1 (S1): WPCBs mechanical treatment, WERP safe landfill; (2) scenario 2 (S2): WPCBs mechanical treatment, WERP imitation stone bricks production. Based on the material flow analysis and comprehensive evaluation, the most profitable and environmentally friendly scenario was selected and assumed to be promoted in Jiangsu area and China from 2013 to 2029. The analysis result showed that S2 had the best economic performance and polybrominated diphenyl ethers (PBDEs) emission reductions potential. S2 is the best option that can gradually replace the traditional recycling model. With the promotion of S2, China would reduce the emissions of PBDEs by 700.8 kg. Meanwhile, it could save $542.2 million in WERP landfill costs, produce 1260.2 kt of imitation stone bricks, and generate $2308.5 million in economic benefits. In conclusion, this study can offer a new idea for dismantling products treatment of household e-waste and provide scientific knowledge to improve the sustainable management.

Neuroprotective Effects of Poria cocos (Agaricomycetes) Essential Oil on Aß1-40-Induced Learning and Memory Deficit in Rats.

Alzheimer's disease (AD) is a neurodegenerative disease that has become a leading cause of death in recent years. The present study aimed to explore the possible prophylactic effects of Poria cocos essential oil (PCEO) against memory deficits in Aß rats. Adult male Wistar rats were given Aß1-42 via ICV injection. The effect of 30 d administration of PCEO by oral gavage was investigated. Novel object recognition (NOR) test, Morris water maze (MWM) test, and passive avoidance memory retention (PAM) task were performed. Aß decreased the cognitive memory in NOR, spatial memory in MWM, and passive avoidance memory in PAM tests. In contrast, PCEO improved learning and memory in the treated group. The PCEO treatment halts the activity of AChE in the hippocampus and cortex of the AD rats. The central neuronal degeneration in Aß-injected rats was not only ascertained by the histopathological changes but also confirmed indirectly by the concomitant increase in GFAP immunostaining. The beneficial effects in AD of increasing cellular GPx, GR, CAT, Na+ K+ ATPase and GST through the administration of PCEO may not only result in protection against neurodegeneration but also result in improvement in cognitive function. PCEO may be recommended as a prophylactic and/or adjunct medication for neurodegenerative diseases.

Hydrothermal oxidation degradation of dioxins in fly ash with water-washing and added Ce-Mn catalyst.

A comprehensive analysis of the effects of the temperature, reaction time, liquid-solid ratio (L/S), and initial pH on the hydrothermal degradation of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) (which are both PCDD/Fs) in municipal solid waste incineration (MSWI) fly ash is presented. Consequently, the hydrothermal degradation reaction is catalyzed using Ce-Mn catalyst under low-temperature conditions to study the effect of the catalyst on the degradation efficiency of PCDD/Fs. The experimental results show that temperature is the most critical factor for the reaction. When the hydrothermal oxidation temperature reaches 280 °C (reaction time = 120 min, original pH = 8.5, L/S = 4 mL/g), the toxicity equivalent (I-TEQ) of PCDD/Fs is only 5.4 ng TEQ/kg, and the degradation efficiency reaches 99.71%. Under these conditions, 2,3,4,7,8-P5CDF makes the highest contribution to I-TEQ degradation, reaching 37.4%. There are four main pathways for the reaction of 2,3,4,7,8-P5CDF with hydroxyl radicals. A comparison of the PCDD/F concentrations of different products shows that the addition of 0.5%, 1.0%, and 1.5% of the Ce-Mn catalyst reduces the degradation efficiency by 8.79%, 1.40%, and 0.07%, respectively, which indicates that the addition of a small quantity of Ce-Mn catalyst does not facilitate the degradation of PCDD/Fs. The addition of the catalyst significantly decreases the degradation efficiency of low-chlorinated homologs but has a relatively small effect on that of high-chlorinated homologs. Therefore, it is concluded that Ce-Mn catalysts are more likely to promote resynthesis than degradation of PCDD/Fs.

Reduced Utilization Process Leaching Toxicity by Stabilizing Heavy Metals in Fly Ash from MSW Incineration Based on Hydrothermal Oxidation.

Hydrothermal oxidation is an effective approach to reduce leaching toxicity of fly ash from municipal solid waste incineration during utilization process. Herein, the effects of temperature, time, pH and Ce-Mn catalyst dosage on the stabilization of heavy metals in fly ash during hydrothermal oxidation were studied. The temperature of hydrothermal oxidation was positively correlated to the stabilization effect. However, the reaction time and pH emerged unstable effect. The amount of Ce-Mn catalyst had a slightly positive effect on the stabilizing at low doses, but it can be inhibited by excessive dose. The leaching concentrations of all heavy metals accorded with National Wastewater Discharge Standard of China (GB 8978-1996) under the optimal parameters, especially for Pb, Ni and Cu. The heavy metals were transformed from unstable fractions to residue fractions during hydrothermal process, among which the proportion of Cu and Zn residue fractions significantly increased and further reduced leaching toxicity.

A case of atypical reninoma with mild hypertension and normal plasma renin activity but elevated plasma renin concentration.

BACKGROUND: Reninoma is a rare, benign renal neoplasm. Typical clinical features include severe hypertension, secondary hyperaldosteronism, hypokalaemia and metabolic alkalosis caused by the overproduction of renin. CASE PRESENTATION: A 25-year-old lean Chinese woman with no family history of hypertension was hospitalized for stage 1 hypertension that gradually developed over two years. Endocrine investigation showed hyperreninemia without hyperaldosteronism and hypokalaemia. Interestingly, although the patient had an elevated plasma renin concentration (PRC), her plasma renin activity (PRA) was in the normal range. Abdominal contrast-enhanced computed tomography (CT) scanning revealed a solid, low-density, renal cortical mass with delayed enhancement. Selective renal vein sampling (SRVS) was performed, and a lateralization of the renin secretion from the left kidney was found. Enucleation of the tumour led to a rapid remission of hypertension and hyperreninemia. Based on pathological findings, the patient was diagnosed with reninoma. Immunohistochemical staining of the tumour was positive for Renin, CD34, Vimentin, and synaptophysin (Syn) and negative for somatostatin receptor 2 (SSTR2) and chromogranin A (CgA). CONCLUSIONS: Reninoma can present as mild hypertension without hyperaldosteronism and hypokalaemia. The clinical features of reninoma may depend on the degree of activation of the renin-angiotensin-aldosterone system (RAAS). PRC should be incorporated in the differential diagnosis of secondary hypertension.

Analysis of mercury emissions and cycles in typical industrial city clusters: a case study in China.

China's Suzhou (SZ), Wuxi (WX), and Changzhou (CZ) (collectively referred to as the SXC area) have developed economies and advanced industrial production, which are typical industrial city clusters. To analyze the Hg flow characteristics, we quantified the Hg emissions and circulation according to six categories (including industrial production, agricultural livestock, vehicle exhaust, solid waste, atmospheric deposition, and runoff). The results showed that the Hg emission from coal accounting for 40.99% of the total circulation. The amount of Hg circulating in SZ is obviously higher than those in WX and CZ, accounting for 47.88% of the total regional emissions. The Hg pollution in SXC area represent an optimistic level. Except that the Hg concentration in the soil in WX is slightly higher than the first-level soil quality standard, the water and atmosphere in the three cities and the soil in SZ and CZ all meet the highest national standards. The study provides in-depth statistics on the Hg cycle characteristics of typical industrial urban agglomerations. It is beneficial to the management of Hg and provides a basis for the implementation of different schemes in different stages of production and emission, so as to effectively prevent the occurrence of serious heavy metal poisoning hazards. This research idea is widely used and can be applied to other regions and other heavy metal elements.

Characteristics and long-term prognosis of patients with reduced, mid-range, and preserved ejection fraction: A systemic review and meta-analysis.

AIMS: Patients with heart failure (HF) have a poor prognosis and are categorized by ejection fraction. We performed a meta-analysis to compare baseline characteristics and long-term outcomes of patients with heart failure with reduced (HFrEF), mid-range (HFmrEF), and preserved ejection fraction (HFpEF). METHODS AND RESULTS: A total of 27 prospective studies were included. Patients with HFpEF were older and had a higher proportion of females, hypertension, diabetes, and insufficient neuroendocrine antagonist treatments, while patients with HFrEF and HFmrEF had a higher prevalence of coronary heart disease and chronic kidney disease. After more than 1-year of follow-up, all-cause mortality was significantly lower in patients with HFmrEF 9388/25 042 (37.49%) than those with HFrEF 39 333/90 023 (43.69%) and HFpEF 24 828/52 492 (47.30%) (p < .001). Cardiovascular mortality was lowest in patients with HFpEF 1130/9904 (11.41%), highest in patients with HFrEF 3419/16 277 (21.07%) mainly coming from HF death and sudden cardiac death, and middle in patients with HFmrEF 699/5171 (13.52%) and the non-cardiovascular mortality was on the contrary. Subgroup analysis showed that in high-risk patients with atrial fibrillation, the all-cause mortality of HFpEF was significantly higher than both HFrEF and HFmrEF (p < .001). HF hospitalization was lowest in patients with HFmrEF 1822/5285 (34.47%), highest in patients with HFrEF 12 607/28 590 (44.10%) and middle in patients with HFpEF 8686/22 763 (38.16%) and the composite of all-cause mortality and HF hospitalization was also observed similar results. CONCLUSIONS: In summary, patients with HFmrEF had the lowest incidence of all-cause mortality and HF hospitalization, while the highest all-cause mortality and HF hospitalization rates were HFpEF and HFrEF patients, respectively.

Co-utilization of lake sediment and blue-green algae for porous lightweight aggregate (ceramsite) production.

Lake sediment and algal sludge with large output posed significant environmental risks. In this work, an idea of co-utilization of both solid wastes for the production of ceramsite (a sort of porous lightweight aggregates as building materials) was proposed and validated for the first time. The treatment process contained a dewatering step by a flocculation-pressure filtration method, and a sintered ceramsite preparation step. Effects of flocculant type and dosage on the dewatering performance were studied in the first step. An environmental-friendly amphoteric starch flocculant with a dosage of 12 mg/(g dried sample) was found to achieve the best dewatering performance. Effects of raw material mass ratio, sintering temperature and time in the second step were investigated. Under the optimal conditions (60 wt% of dewatered sediment; 20 wt% of dewatered algal sludge; 20 wt% of additives (fly ash: calcium oxide: kaolin = 2:1:2); sintering temperature: 1100 °C; time: 35 min), the obtained ceramsite met the Chinese National Standard as a qualified building material, with reliable environmental safety according to the leaching results for both heavy metals and microcystins. Both environmental and economic benefits of the proposed treatment were assessed. The process completely followed the rules of "reduction, harmlessness and resource utilization" for solid waste treatment and disposal; Meanwhile, the profit of the proposed ceramsite production could be more than 2.3 US dollar/m3. The co-utilization method in this work acted as a good example for the comprehensive management of solid wastes in water-rich areas.

The change of water content and role of microbe in the sludge drying process.

In this study, the pure terephthalic acid sludge was treated by a drying process which was an economical and environmentally method. The water change and metabolic pathways for bacterial community function prediction were analyzed. The microbial community changes were studied by high-throughput sequencing to draw the dynamic succession model. Then multiple statistics was used to determine the key factors of the reduction. The results showed that the main stage of water loss in the PTA sludge drying process was the high temperature period where the water lost by evaporation accounted for more than 90% of the total removal. The main metabolic pathways for bacterial community function were amino acid (7.72%-8.71% of Kyoto encyclopedia of genes and genomes relative abundance and 8.26%-9.51% of Cluster of orthologous groups of proteins relative abundance) and carbohydrate metabolism. The model describing the dynamic succession of microbial communities showed that the dominant bacteria changed from Nitrospira, Novosphingobium and Azohydromonas to Pseudomonas, Paeniglutamicibac and Pelotomaculum. The key factors for water loss were Gemmatimonas, Novosphingobium and Azohydromonas with the correlation coefficients of 0.887, 0.772, 0.783, respectively; the key factors for dry matter loss were Pelotomaculum, total organic matter, dissovlved organic carbon and carbon/nitrogen ratio; the key factors for toxic substance loss were Brevundimonas, Novosphingobium and Gemmatimonas. These results provided theoretical support for the application and demonstration for hazardous waste sludge reduction.

Performance and mechanism of high-speed vermicomposting of dewatered sludge using a new type of laboratory earthworm reactor.

To solve the problem of the traditional vermicomposting cycle being too long, a new type of laboratory earthworm reactor was developed for high-speed vermicomposting of sludge. The earthworm reactor was established based on the model of first creating an optimal living environment for earthworms and then introducing sludge into the environment for vermicomposting. In addition, we selected four different materials to condition sludge to optimize the treatment efficiency and shorten the vermicomposting cycle. The results revealed that the use of the new earthworm reactor for high-speed vermicomposting can shorten the vermicomposting cycle to 61.33 h, which is 1/30 of the traditional method. Compared to the traditional method, the vermicompost obtained from high-speed vermicomposting had better stability and maturity (C/N: 14.96, humification index: 4.69, Germination index: 78.84%, TOC: 88.5 mg/g and ash content: 686 mg/g). Besides, the FT-IR, SEM, EEM, and enzyme activity from the earthworm analysis results show that the addition of vermicompost (raw material) was beneficial to the stability and mineralization of the final vermicompost for dewatered sludge vermicomposting.

Pilot-scale vermicomposting of sewage sludge mixed with mature vermicompost using earthworm reactor of frame composite structure.

To improve the efficiency of sludge vermicomposting, a new cost-effective method is provided. It uses a new earthworm reactor with a frame composite structure for vermicomposting and reuses mature vermicompost to condition the sludge. Under the optimum conditions (proportion of earthworm droppings: 15%; thickness of sludge laying: 6 cm; moisture content of initial sludge mixture: 75%), the method of continuous operation described herein works well and presents three advantages compared with the traditional vermicomposting method: the short time required for vermicomposting (20.25 h); covering a small area (5 m2/t·d); and a low cost. In addition, the vermicompost obtained from sludge vermicomposting shows better stability and maturity (C/N: 14.96; GI: 86.42%; TOC: 188.5 mg/g; ash content: 516.2 mg/g). The investigation of the associated mechanisms, including 3D-EEM, TGA, SEM and microbial community analyses, revealed that the addition of mature vermicompost can speed up the progress of decomposition and humification of organic matter in sludge. The process of vermicomposting and adding mature vermicompost significantly modified the microbial community of sewage sludge, and the changes in microorganisms in vermicompost were related to the microorganisms in the earthworm gut.

Full-scale thermophilic aerobic co-composting of blue-green algae sludge with livestock faeces and straw.

A high incidence of harmful algal bloom in eutrophic surface waters causes many environmental problems. Thermophilic aerobic composting enables effective treatment and disposal of algal sludge that remains after the dewatering of algae slurries, and provides a value-added organic fertiliser. Previous studies have either only dealt with the composting of a single waste component or were conducted at a lab-/pilot-scale; however, this work is a comprehensive assessment of full-scale mechanized thermophilic aerobic co-composting of algal sludge and other typical biomass-based wastes, including chicken faeces and rice straw, in a water-rich rural area in the Tai lake basin, China. With the optimised feedstock material mass ratio (6.0:1.8:1.0 for straw:algae:faeces; initial C/N ratio of 20; and initial moisture of 60 wt%), the co-composting process effectively achieved the reduction, harmlessness, and reuse of waste. The moisture content (28.36 wt% of wet weight), organic matter content (57.91 wt% of dried weight), total nutrient content (6.59 wt% for TN + TP + TK of dried weight), and heavy metal contents as well as the pH of the final product fully met the Chinese National Agricultural Organic Fertiliser Standard requirements. The reduction rates of microcystin and toxic volatile fatty acid contents were higher than 99.5%, and the seed germination index of the product was 114.5%. A notable economic benefit with a gross profit margin of 167-434% of the process was highlighted. Investigation of the associated mechanisms, including statistical analysis, spectral characterisation, micro-morphological observation, and microbial community analysis, revealed that a decreased particle sizes with a looser structure and an efficient humification effect, resulting from the work of several identified dominant microbial species, contributed to the high product quality. The current study provided a demonstration of the promising full-scale co-composting technology for comprehensive management of the environment in water-rich rural areas and the construction of a sustainable watershed.

Intensity analysis of chromium cycling in south Jiangsu region of China.

Suzhou (SZ), Wuxi (WX) and Changzhou (CZ) (collectively called the SXC area) in southern Jiangsu Province surround Tai Lake on three sides and have an important impact on its ecology. The emission and circulation of Cr in the three cities were quantified according to the six categories (including industry production, agricultural livestock, vehicle exhaust, solid waste, atmospheric subsidence and runoff) to analyze its regional characteristics and source category characteristics and to build a Cr cycle diagram to evaluate the pollution situation. The results showed that the Cr emissions from solid waste were the highest and mostly came from industrial sludge, accounting for 76.4% of the total circulation. The Cr emissions from SZ and WX were significantly higher than those of CZ, accounting for 47.0% and 42.9% of the regional total. The Cr in the excrement of pigs and poultry, dry sedimentation and surface runoff exceeded 100 tons every year, which needed to be valued. The Cr concentration in the surface water, soil and atmosphere in SXC area all met with the highest national standards. Studies have shown that the sediments and benthic organisms in the west and north of Tai Lake were already in a low-pollution state, but which was overall acceptable. Through this study, Cr circulation was clarified in typical areas, which was convenient for the monitoring and management of heavy metal pollution in the areas surrounding Tai Lake.

Identification of a novel SDHB c.563 T > C mutation responsible for Paraganglioma syndrome and genetic analysis of the SDHB gene in China: a case report.

BACKGROUND: Pheochromocytoma/paraganglioma (PPGL) is a rare neuroendocrine tumor. Succinate dehydrogenase (SDH) deficiency has been confirmed to be associated with PPGL in various studies. SDHB mutations play an important role in PPGL. However, genetic screening of PPGL patients has not been widely carried out in clinics in China, and only a few related studies have been reported. CASE PRESENTATION: We report a case of a 23-year-old woman with paraganglioma (PGL) caused by a novel missense SDHB mutation, c.563 T > C (p.Leu188Pro), who presented with paroxysmal hypertension. Computed tomography (CT) and magnetic resonance imaging (MRI) revealed a PGL in the right retroperitoneum and no metastasis. The patient was treated with surgical excision and did not have postsurgerical paroxysmal hypertension. In addition, we searched the literature related to variations in SDHB genes in Chinese patients with PPGL using multiple online databases, including PubMed, China Hospital Knowledge Database and Wanfang Data. Ultimately, 14 studies (published between 2006 and 2019) comprising 34 cases of SDHB-related PGL or pheochromocytoma (PCC) were found. In total, 35 patients were enrolled in this study, and 25 mutations were identified. The common genetic alterations of SDHB in China were c.136C > T (11.4%), c.18C > A (11.4%) and c.725G > A (8.5%). Some carriers of SDHB mutations (28.1%) developed metastatic PPGL, and a high frequency of head and neck PGLs (HNPGLs) (59.4%) was reported. CONCLUSIONS: We describe a classic case with a novel SDHB c.563 T > C mutation. Based on our literature review, common SDHB gene mutations in Chinese PPGL patients are c.136C > T, c.18C > A and c.725G > A.

Determinants of carotid intima-media thickness in asymptomatic elders: a population-based cross-sectional study in rural China.

Objective: To examine the mean carotid intima-media thickness (CIMT) and potentially relevant determinants of increased CIMT in elders. Method: Stroke-free and cardiovascular disease-free residents aged ≥65 years were recruited in a low-income population in China. B-mode ultrasonography was performed to measure CIMT. Results: A total of 1039 individuals (47.9% men) were recruited. The mean CIMT value was 0.60 (SD: 0.09) mm. The mean CIMT was 24.07 (SEM: 6.52) µm greater in men than in women (P < 0.001) and 28.29 (SEM: 7.47) µm greater in patients with hypertension than in those without hypertension (P < 0.001). Moreover, the mean CIMT increased by 1.53 (SEM: 0.49) µm for each 1-year increase in age (P = 0.002). However, the mean CIMT decreased by 5.55 (SEM: 2.40) µm and 6.45 (SEM: 2.62) µm for every 1-mmol/L increase in triglyceride concentration and the high-density lipoprotein cholesterol: low-density lipoprotein cholesterol ratio, respectively (P < 0.05). However, high triglyceride level was negatively associated with mean CIMT only among individuals without metabolic syndrome (P = 0.036). Discussion: These findings suggest that there is an urgent need to delay atherosclerosis progression and reduce the stroke burden by managing hypertension, especially for men. Moreover, to decrease the stroke burden in rural China, caution is advised regarding lipid-lowering treatment in elderly patients without metabolic syndrome.