RÉSUMÉ
It has been hypothesized that oils containing high levels of omega-3 polyunsaturated fatty acids, such as canola and fish oil, could counteract some of the adverse effects induced by phthalates. In the present study, the influence of different oily vehicles on di-butyl phthalate (DBP)-induced testicular toxicity and lipid profile was investigated. Pregnant Wistar rats were treated by oral gavage from gestation days 13 to 20 with DBP (500 mg/kg/day) diluted in three different vehicles: corn, canola or fish oil. Male fetuses were analyzed on gestation day 20. DBP exposure lowered intratesticular testosterone levels and anogenital distance, regardless of the vehicle used. The percentage of seminiferous cords containing multinucleated gonocytes and cord diameter was increased in DBP-exposed groups, compared with vehicle controls, with no difference between the three DBP-exposed groups. Clustering of Leydig cells was seen in all DBP groups. Lipid profile indicated that administration of canola and fish oil can increase the content of omega-3 fatty acids in rat testis. However, content of omega-3 was diminished in DBP-treated groups. Overall, our results indicate that different oily vehicles did not alter fetal rat testicular toxicity induced by a high DBP dose.
Sujet(s)
Phtalate de dibutyle/toxicité , Perturbateurs endocriniens/toxicité , Acides gras omega-3/métabolisme , Métabolisme lipidique/effets des médicaments et des substances chimiques , Exposition maternelle/effets indésirables , Véhicules pharmaceutiques/métabolisme , Testicule/effets des médicaments et des substances chimiques , Animaux , Huile de maïs/composition chimique , Huile de maïs/métabolisme , Phtalate de dibutyle/administration et posologie , Perturbateurs endocriniens/administration et posologie , Polluants environnementaux/administration et posologie , Polluants environnementaux/toxicité , Acides gras monoinsaturés/composition chimique , Acides gras monoinsaturés/métabolisme , Acides gras omega-3/composition chimique , Femelle , Développement foetal/effets des médicaments et des substances chimiques , Huiles de poisson/composition chimique , Huiles de poisson/métabolisme , Mâle , Véhicules pharmaceutiques/composition chimique , Plastifiants/administration et posologie , Plastifiants/toxicité , Grossesse , Huile de colza , Rats , Processus de détermination du sexe/effets des médicaments et des substances chimiques , Testicule/embryologie , Testicule/métabolisme , Testostérone/métabolismeRÉSUMÉ
Artemisinins combination therapy (ACT) is the first choice therapy for falciparum malaria. Data on the safety of ACTs in pregnancy are limited and controversial and the use is not recommended on the first trimester. To evaluate the effects of isolated and combined artesunate (AS)/mefloquine (MQ) on embryo rats, pregnant rats were treated orally with AS (15 and 40 mg/kg body weight (bwt)/day), MQ (30 and 80 mg/kg bwt/day) and AS/MQ (15/30 and 40/80 mg/kg bwt/day) on days 9-11 post coitum (pc). The dams were euthanized on day 12 pc and gestational and embryos histological parameters were evaluated. Embryolethality and histopathological anomalies were significant when AS was given alone or combined with MQ. Combination of AS and MQ did not enhance their toxicity compared to their separate administrations; on the other side, there was a reduction in the toxic effects of the AS when combined with MQ. Isolated MQ did not induce developmental toxicity.
