RÉSUMÉ
PURPOSE:: To investigate the potential protective effects of enoxaparin against the adverse events of carbon dioxide (CO2) pneumoperitoneum. METHODS:: Thirty four rats were divided into three groups: Group 1 (sham) underwent insertion of Veress needle into the abdomen and 90 min of anesthesia with no gas insufflation. The animals in control and enoxaparin groups were subjected to 90 min of 14 mmHg CO2 pneumoperitoneum. Enoxaparin (100 u/kg) was administered subcutaneously to the rats in enoxaparin group one hour before the operation. After 90 min of pneumoperitoneum, the rats were allowed for reperfusion through 60 min. Blood and liver samples were obtained for biochemical and histopathological examination. RESULTS:: Treatment with enoxaparin decreased the histopathological abnormalities when compared with the control group. The highest levels of oxidative stress parameters were found in control group. The use of enoxaparin decreased the levels of all oxidative stress parameters, but the difference between the control and enoxaparin groups was not statistically significant. CONCLUSION:: Enoxaparin ameliorated the harmful effects of high pressure CO2 pneumoperitoneum on the liver.
Sujet(s)
Anticoagulants/usage thérapeutique , Dioxyde de carbone/effets indésirables , Énoxaparine/usage thérapeutique , Foie/effets des médicaments et des substances chimiques , Oxygène/administration et posologie , Pneumopéritoine artificiel/effets indésirables , Animaux , Dioxyde de carbone/administration et posologie , Modèles animaux de maladie humaine , Femelle , Foie/anatomopathologie , Stress oxydatif/physiologie , Pneumopéritoine artificiel/méthodes , Pression , Rats , Rat Wistar , Thromboembolie/prévention et contrôleRÉSUMÉ
ABSTRACT PURPOSE: To investigate the potential protective effects of enoxaparin against the adverse events of carbon dioxide (CO2) pneumoperitoneum. METHODS: Thirty four rats were divided into three groups: Group 1 (sham) underwent insertion of Veress needle into the abdomen and 90 min of anesthesia with no gas insufflation. The animals in control and enoxaparin groups were subjected to 90 min of 14 mmHg CO2 pneumoperitoneum. Enoxaparin (100 u/kg) was administered subcutaneously to the rats in enoxaparin group one hour before the operation. After 90 min of pneumoperitoneum, the rats were allowed for reperfusion through 60 min. Blood and liver samples were obtained for biochemical and histopathological examination. RESULTS: Treatment with enoxaparin decreased the histopathological abnormalities when compared with the control group. The highest levels of oxidative stress parameters were found in control group. The use of enoxaparin decreased the levels of all oxidative stress parameters, but the difference between the control and enoxaparin groups was not statistically significant. CONCLUSION: Enoxaparin ameliorated the harmful effects of high pressure CO2 pneumoperitoneum on the liver.
Sujet(s)
Animaux , Femelle , Rats , Oxygène/administration et posologie , Pneumopéritoine artificiel/effets indésirables , Dioxyde de carbone/effets indésirables , Énoxaparine/usage thérapeutique , Foie/effets des médicaments et des substances chimiques , Anticoagulants/usage thérapeutique , Pneumopéritoine artificiel/méthodes , Pression , Thromboembolie/prévention et contrôle , Dioxyde de carbone/administration et posologie , Rat Wistar , Stress oxydatif/physiologie , Modèles animaux de maladie humaine , Foie/anatomopathologieRÉSUMÉ
PURPOSE:To investigate the potential protective effects of enoxaparin against the adverse events of carbon dioxide (CO2) pneumoperitoneum.METHODS:Thirty four rats were divided into three groups: Group 1 (sham) underwent insertion of Veress needle into the abdomen and 90 min of anesthesia with no gas insufflation. The animals in control and enoxaparin groups were subjected to 90 min of 14 mmHg CO2 pneumoperitoneum. Enoxaparin (100 u/kg) was administered subcutaneously to the rats in enoxaparin group one hour before the operation. After 90 min of pneumoperitoneum, the rats were allowed for reperfusion through 60 min. Blood and liver samples were obtained for biochemical and histopathological examination.RESULTS:Treatment with enoxaparin decreased the histopathological abnormalities when compared with the control group. The highest levels of oxidative stress parameters were found in control group. The use of enoxaparin decreased the levels of all oxidative stress parameters, but the difference between the control and enoxaparin groups was not statistically significant.CONCLUSION:Enoxaparin ameliorated the harmful effects of high pressure CO2 pneumoperitoneum on the liver.(AU)