RÉSUMÉ
Plant genomes encode hundreds of receptor kinases and peptides, but the number of known plant receptor-ligand pairs is limited. We report that the Arabidopsis leucine-rich repeat receptor kinase LRR-RK MALE DISCOVERER 1-INTERACTING RECEPTOR LIKE KINASE 2 (MIK2) is the receptor for the SERINE RICH ENDOGENOUS PEPTIDE (SCOOP) phytocytokines. MIK2 is necessary and sufficient for immune responses triggered by multiple SCOOP peptides, suggesting that MIK2 is the receptor for this divergent family of peptides. Accordingly, the SCOOP12 peptide directly binds MIK2 and triggers complex formation between MIK2 and the BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) co-receptor. MIK2 is required for resistance to the important root pathogen Fusarium oxysporum. Notably, we reveal that Fusarium proteomes encode SCOOP-like sequences, and corresponding synthetic peptides induce MIK2-dependent immune responses. These results suggest that MIK2 may recognise Fusarium-derived SCOOP-like sequences to induce immunity against Fusarium. The definition of SCOOPs as MIK2 ligands will help to unravel the multiple roles played by MIK2 during plant growth, development and stress responses.
Sujet(s)
Protéines d'Arabidopsis/métabolisme , Arabidopsis/immunologie , Protéines fongiques/métabolisme , Protéines et peptides de signalisation intercellulaire/métabolisme , Maladies des plantes/immunologie , Protein kinases/métabolisme , Récepteurs de surface cellulaire/métabolisme , Arabidopsis/métabolisme , Arabidopsis/microbiologie , Protéines d'Arabidopsis/immunologie , Clonage moléculaire , Résistance à la maladie/immunologie , Protéines fongiques/immunologie , Fusarium/immunologie , Fusarium/métabolisme , Fusarium/pathogénicité , Protéines et peptides de signalisation intercellulaire/immunologie , Ligands , Maladies des plantes/microbiologie , Racines de plante/immunologie , Racines de plante/métabolisme , Racines de plante/microbiologie , Végétaux génétiquement modifiés , Protein kinases/immunologie , Protein-Serine-Threonine Kinases/métabolisme , Récepteurs de surface cellulaire/immunologie , Transduction du signal/immunologie , Nicotiana/génétique , Nicotiana/immunologieRÉSUMÉ
Arabidopsis thaliana accessions are universally resistant at the adult leaf stage to white rust (Albugo candida) races that infect the crop species Brassica juncea and Brassica oleracea We used transgressive segregation in recombinant inbred lines to test if this apparent species-wide (nonhost) resistance in A. thaliana is due to natural pyramiding of multiple Resistance (R) genes. We screened 593 inbred lines from an Arabidopsis multiparent advanced generation intercross (MAGIC) mapping population, derived from 19 resistant parental accessions, and identified two transgressive segregants that are susceptible to the pathogen. These were crossed to each MAGIC parent, and analysis of resulting F2 progeny followed by positional cloning showed that resistance to an isolate of A. candida race 2 (Ac2V) can be explained in each accession by at least one of four genes encoding nucleotide-binding, leucine-rich repeat (NLR) immune receptors. An additional gene was identified that confers resistance to an isolate of A. candida race 9 (AcBoT) that infects B. oleracea Thus, effector-triggered immunity conferred by distinct NLR-encoding genes in multiple A. thaliana accessions provides species-wide resistance to these crop pathogens.
Sujet(s)
Arabidopsis/immunologie , Brassica/microbiologie , Oomycetes/pathogénicité , Maladies des plantes/immunologie , Arabidopsis/génétique , Arabidopsis/microbiologie , Protéines d'Arabidopsis/génétique , Régulation de l'expression des gènes végétaux , Gènes de plante , Haplotypes , Immunité innée , Maladies des plantes/microbiologieRÉSUMÉ
Chitin, a major component of fungal cell walls, is a well-known pathogen-associated molecular pattern (PAMP) that triggers defense responses in several mammal and plant species. Here, we show that two chitooligosaccharides, chitin and chitosan, act as PAMPs in grapevine (Vitis vinifera) as they elicit immune signalling events, defense gene expression and resistance against fungal diseases. To identify their cognate receptors, the grapevine family of LysM receptor kinases (LysM-RKs) was annotated and their gene expression profiles were characterized. Phylogenetic analysis clearly distinguished three V. vinifera LysM-RKs (VvLYKs) located in the same clade as the Arabidopsis CHITIN ELICITOR RECEPTOR KINASE1 (AtCERK1), which mediates chitin-induced immune responses. The Arabidopsis mutant Atcerk1, impaired in chitin perception, was transformed with these three putative orthologous genes encoding VvLYK1-1, -2, or -3 to determine if they would complement the loss of AtCERK1 function. Our results provide evidence that VvLYK1-1 and VvLYK1-2, but not VvLYK1-3, functionally complement the Atcerk1 mutant by restoring chitooligosaccharide-induced MAPK activation and immune gene expression. Moreover, expression of VvLYK1-1 in Atcerk1 restored penetration resistance to the non-adapted grapevine powdery mildew (Erysiphe necator). On the whole, our results indicate that the grapevine VvLYK1-1 and VvLYK1-2 participate in chitin- and chitosan-triggered immunity and that VvLYK1-1 plays an important role in basal resistance against E. necator.
Sujet(s)
Protéines d'Arabidopsis/métabolisme , Ascomycota/physiologie , Chitine/analogues et dérivés , Maladies des plantes/immunologie , Immunité des plantes/génétique , Protein-Serine-Threonine Kinases/métabolisme , Vitis/enzymologie , Arabidopsis/enzymologie , Arabidopsis/génétique , Arabidopsis/immunologie , Protéines d'Arabidopsis/génétique , Chitine/métabolisme , Chitine/pharmacologie , Chitosane , Oligosaccharides , Phylogenèse , Maladies des plantes/microbiologie , Protéines végétales/génétique , Protéines végétales/métabolisme , Protein-Serine-Threonine Kinases/génétique , Vitis/génétique , Vitis/immunologieRÉSUMÉ
Plants are constantly exposed to would-be pathogens and pests, and thus have a sophisticated immune system to ward off these threats, which otherwise can have devastating ecological and economic consequences on ecosystems and agriculture. Plants employ receptor kinases (RKs) and receptor-like proteins (RLPs) as pattern recognition receptors (PRRs) to monitor their apoplastic environment and detect non-self and damaged-self patterns as signs of potential danger. Plant PRRs contribute to both basal and non-host resistances, and treatment with pathogen-/microbe-associated molecular patterns (PAMPs/MAMPs) or damage-associated molecular patterns (DAMPs) recognized by plant PRRs induces both local and systemic immunity. Here, we comprehensively review known PAMPs/DAMPs recognized by plants as well as the plant PRRs described to date. In particular, we describe the different methods that can be used to identify PAMPs/DAMPs and PRRs. Finally, we emphasize the emerging biotechnological potential use of PRRs to improve broad-spectrum, and potentially durable, disease resistance in crops.
Sujet(s)
Résistance à la maladie/génétique , Immunité des plantes , Protéines végétales/génétique , Récepteurs de reconnaissance de motifs moléculaires/génétique , Produits agricolesRÉSUMÉ
Plants actively perceive and respond to perturbations in their cell walls which arise during growth, biotic and abiotic stresses. However, few components involved in plant cell wall integrity sensing have been described to date. Using a reverse-genetic approach, we identified the Arabidopsis thaliana leucine-rich repeat receptor kinase MIK2 as an important regulator of cell wall damage responses triggered upon cellulose biosynthesis inhibition. Indeed, loss-of-function mik2 alleles are strongly affected in immune marker gene expression, jasmonic acid production and lignin deposition. MIK2 has both overlapping and distinct functions with THE1, a malectin-like receptor kinase previously proposed as cell wall integrity sensor. In addition, mik2 mutant plants exhibit enhanced leftward root skewing when grown on vertical plates. Notably, natural variation in MIK2 (also named LRR-KISS) has been correlated recently to mild salt stress tolerance, which we could confirm using our insertional alleles. Strikingly, both the increased root skewing and salt stress sensitivity phenotypes observed in the mik2 mutant are dependent on THE1. Finally, we found that MIK2 is required for resistance to the fungal root pathogen Fusarium oxysporum. Together, our data identify MIK2 as a novel component in cell wall integrity sensing and suggest that MIK2 is a nexus linking cell wall integrity sensing to growth and environmental cues.
Sujet(s)
Protéines d'Arabidopsis/génétique , Paroi cellulaire/génétique , Racines de plante/génétique , Protein kinases/génétique , Récepteurs de surface cellulaire/génétique , Stress physiologique/génétique , Arabidopsis/effets des médicaments et des substances chimiques , Arabidopsis/génétique , Protéines d'Arabidopsis/biosynthèse , Paroi cellulaire/effets des médicaments et des substances chimiques , Cellulose/biosynthèse , Cyclopentanes/métabolisme , Résistance à la maladie/génétique , Fusarium/pathogénicité , Régulation de l'expression des gènes végétaux/effets des médicaments et des substances chimiques , Lignine/biosynthèse , Oxylipines/métabolisme , Maladies des plantes/génétique , Maladies des plantes/microbiologie , Racines de plante/effets des médicaments et des substances chimiques , Protein kinases/biosynthèse , Chlorure de sodium/toxicité , Stress physiologique/effets des médicaments et des substances chimiquesRÉSUMÉ
The plant cell wall, often the site of initial encounters between plants and their microbial pathogens, is composed of a complex mixture of cellulose, hemicellulose, and pectin polysaccharides as well as proteins. The concept of damage-associated molecular patterns (DAMPs) was proposed to describe plant elicitors like oligogalacturonides (OGs), which can be derived by the breakdown of the pectin homogalacturon by pectinases. OGs act via many of the same signaling steps as pathogen- or microbe-associated molecular patterns (PAMPs) to elicit defenses and provide protection against pathogens. Given both the complexity of the plant cell wall and the fact that many pathogens secrete a wide range of cell wall-degrading enzymes, we reasoned that the breakdown products of other cell wall polymers may be similarly biologically active as elicitors and may help to reinforce the perception of danger by plant cells. Our results indicate that oligomers derived from cellulose are perceived as signal molecules in Arabidopsis (Arabidopsis thaliana), triggering a signaling cascade that shares some similarities to responses to well-known elicitors such as chitooligomers and OGs. However, in contrast to other known PAMPs/DAMPs, cellobiose stimulates neither detectable reactive oxygen species production nor callose deposition. Confirming our idea that both PAMPs and DAMPs are likely to cooccur at infection sites, cotreatments of cellobiose with flg22 or chitooligomers led to synergistic increases in gene expression. Thus, the perception of cellulose-derived oligomers may participate in cell wall integrity surveillance and represents an additional layer of signaling following plant cell wall breakdown during cell wall remodeling or pathogen attack.
Sujet(s)
Arabidopsis/métabolisme , Paroi cellulaire/métabolisme , Cellulose/métabolisme , Oligosaccharides/métabolisme , Arabidopsis/génétique , Arabidopsis/microbiologie , Protéines d'Arabidopsis/génétique , Paroi cellulaire/génétique , Paroi cellulaire/microbiologie , Cellobiose/métabolisme , Diholoside/métabolisme , Résistance à la maladie/génétique , Analyse de profil d'expression de gènes/méthodes , Régulation de l'expression des gènes végétaux , Interactions hôte-pathogène , Mutation , Pectine/métabolisme , Maladies des plantes/génétique , Maladies des plantes/microbiologie , Végétaux génétiquement modifiés , Régions promotrices (génétique)/génétique , Pseudomonas syringae/physiologie , RT-PCR , Plant/génétique , Plant/métabolisme , Plant/microbiologie , Facteurs de transcription/génétiqueRÉSUMÉ
Mitogen-activated protein kinases (MAPKs) mediate plant immune responses to pathogenic bacteria. However, less is known about the cell autonomous negative regulatory mechanism controlling basal plant immunity. We report the biological role of Arabidopsis thaliana MAPK phosphatase AP2C1 as a negative regulator of plant basal resistance and defense responses to Pseudomonas syringae. AP2C2, a closely related MAPK phosphatase, also negatively controls plant resistance. Loss of AP2C1 leads to enhanced pathogen-induced MAPK activities, increased callose deposition in response to pathogen-associated molecular patterns or to P. syringae pv. tomato (Pto) DC3000, and enhanced resistance to bacterial infection with Pto. We also reveal the impact of AP2C1 on the global transcriptional reprogramming of transcription factors during Pto infection. Importantly, ap2c1 plants show salicylic acid-independent transcriptional reprogramming of several defense genes and enhanced ethylene production in response to Pto. This study pinpoints the specificity of MAPK regulation by the different MAPK phosphatases AP2C1 and MKP1, which control the same MAPK substrates, nevertheless leading to different downstream events. We suggest that precise and specific control of defined MAPKs by MAPK phosphatases during plant challenge with pathogenic bacteria can strongly influence plant resistance.
Sujet(s)
Protéines d'Arabidopsis/génétique , Arabidopsis/génétique , Régulation de l'expression des gènes végétaux , Phosphoprotein Phosphatases/génétique , Immunité des plantes , Protein Tyrosine Phosphatases/génétique , Pseudomonas syringae/physiologie , Arabidopsis/immunologie , Arabidopsis/microbiologie , Protéines d'Arabidopsis/métabolisme , Mitogen-Activated Protein Kinases/métabolisme , Phosphoprotein Phosphatases/métabolisme , Protein Tyrosine Phosphatases/métabolismeRÉSUMÉ
High humidity has a strong influence on the development of numerous diseases affecting the above-ground parts of plants (the phyllosphere) in crop fields and natural ecosystems, but the molecular basis of this humidity effect is not understood. Previous studies have emphasized immune suppression as a key step in bacterial pathogenesis. Here we show that humidity-dependent, pathogen-driven establishment of an aqueous intercellular space (apoplast) is another important step in bacterial infection of the phyllosphere. Bacterial effectors, such as Pseudomonas syringae HopM1, induce establishment of the aqueous apoplast and are sufficient to transform non-pathogenic P. syringae strains into virulent pathogens in immunodeficient Arabidopsis thaliana under high humidity. Arabidopsis quadruple mutants simultaneously defective in a host target (AtMIN7) of HopM1 and in pattern-triggered immunity could not only be used to reconstitute the basic features of bacterial infection, but also exhibited humidity-dependent dyshomeostasis of the endophytic commensal bacterial community in the phyllosphere. These results highlight a new conceptual framework for understanding diverse phyllosphere-bacterial interactions.
Sujet(s)
Arabidopsis/microbiologie , Interactions hôte-pathogène , Humidité , Maladies des plantes/microbiologie , Feuilles de plante/microbiologie , Pseudomonas syringae/pathogénicité , Eau/métabolisme , Arabidopsis/immunologie , Arabidopsis/métabolisme , Protéines d'Arabidopsis/métabolisme , Protéines bactériennes/métabolisme , Facteurs d'échange de nucléotides guanyliques , Homéostasie , Tolérance immunitaire , Maladies des plantes/immunologie , Immunité des plantes , Feuilles de plante/immunologie , Pseudomonas syringae/génétique , Pseudomonas syringae/immunologie , Pseudomonas syringae/métabolisme , Symbiose , Virulence/immunologieRÉSUMÉ
Plants are continuously monitoring the presence of microorganisms to establish an adapted response. Plants commonly use pattern recognition receptors (PRRs) to perceive microbe- or pathogen-associated molecular patterns (MAMPs/PAMPs) which are microorganism molecular signatures. Located at the plant plasma membrane, the PRRs are generally receptor-like kinases (RLKs) or receptor-like proteins (RLPs). MAMP detection will lead to the establishment of a plant defense program called MAMP-triggered immunity (MTI). In this review, we overview the RLKs and RLPs that assure early recognition and control of pathogenic or beneficial bacteria. We also highlight the crucial function of PRRs during plant-microbe interactions, with a special emphasis on the receptors of the bacterial flagellin and peptidoglycan. In addition, we discuss the multiple strategies used by bacteria to evade PRR-mediated recognition.
RÉSUMÉ
⢠The role of flagellin perception in the context of plant beneficial bacteria still remains unclear. Here, we characterized the flagellin sensing system flg22-FLAGELLIN SENSING 2 (FLS2) in grapevine, and analyzed the flagellin perception in the interaction with the endophytic plant growth-promoting rhizobacterium (PGPR) Burkholderia phytofirmans. ⢠The functionality of the grapevine FLS2 receptor, VvFLS2, was demonstrated by complementation assays in the Arabidopsis thaliana fls2 mutant, which restored flg22-induced H2O2 production and growth inhibition. Using synthetic flg22 peptides from different bacterial origins, we compared recognition specificities between VvFLS2 and AtFLS2. ⢠In grapevine, flg22-triggered immune responses are conserved and led to partial resistance against Botrytis cinerea. Unlike flg22 peptides derived from Pseudomonas aeruginosa or Xanthomonas campestris, flg22 peptide derived from B. phytofirmans triggered only a small oxidative burst, weak and transient defense gene induction and no growth inhibition in grapevine. Although, in Arabidopsis, all the flg22 epitopes exhibited similar biological activities, the expression of VvFLS2 into the fls2 background conferred differential flg22 responses characteristic for grapevine. ⢠These results demonstrate that VvFLS2 differentially recognizes flg22 from different bacteria, and suggest that flagellin from the beneficial PGPR B. phytofirmans has evolved to evade this grapevine immune recognition system.
Sujet(s)
Burkholderia/physiologie , Endophytes/croissance et développement , Épitopes/immunologie , Flagelline/immunologie , Protéines végétales/métabolisme , Récepteurs de surface cellulaire/métabolisme , Vitis/immunologie , Vitis/microbiologie , Séquence d'acides aminés , Arabidopsis/physiologie , Botrytis/effets des médicaments et des substances chimiques , Botrytis/physiologie , Burkholderia/croissance et développement , Membrane cellulaire/effets des médicaments et des substances chimiques , Membrane cellulaire/métabolisme , Numération de colonies microbiennes , Simulation numérique , Résistance à la maladie/effets des médicaments et des substances chimiques , Endophytes/effets des médicaments et des substances chimiques , Flagelline/pharmacologie , Test de complémentation , Données de séquences moléculaires , Mutation/génétique , Maladies des plantes/immunologie , Maladies des plantes/microbiologie , Immunité des plantes/effets des médicaments et des substances chimiques , Protéines végétales/composition chimique , Espèces réactives de l'oxygène/métabolisme , Récepteurs de surface cellulaire/composition chimique , Spécificité d'espèce , Vitis/effets des médicaments et des substances chimiques , Vitis/croissance et développementRÉSUMÉ
Effective plant defense strategies rely in part on the perception of non-self determinants, so-called microbe-associated molecular patterns (MAMPs), by transmembrane pattern recognition receptors leading to MAMP-triggered immunity. Plant resistance against necrotrophic pathogens with a broad host range is complex and yet not well understood. Particularly, it is unclear if resistance to necrotrophs involves pattern recognition receptors. Here, we partially purified a novel proteinaceous elicitor called sclerotinia culture filtrate elicitor1 (SCFE1) from the necrotrophic fungal pathogen Sclerotinia sclerotiorum that induces typical MAMP-triggered immune responses in Arabidopsis thaliana. Analysis of natural genetic variation revealed five Arabidopsis accessions (Mt-0, Lov-1, Lov-5, Br-0, and Sq-1) that are fully insensitive to the SCFE1-containing fraction. We used a forward genetics approach and mapped the locus determining SCFE1 sensitivity to receptor-like protein30 (RLP30). We also show that SCFE1-triggered immune responses engage a signaling pathway dependent on the regulatory receptor-like kinases brassinosteroid insensitive1-associated receptor kinase1 (BAK1) and Suppressor of BIR1-1/evershed (SOBIR1/EVR). Mutants of RLP30, BAK1, and SOBIR1 are more susceptible to S. sclerotiorum and the related fungus Botrytis cinerea. The presence of an elicitor in S. sclerotiorum evoking MAMP-triggered immune responses and sensed by RLP30/SOBIR1/BAK1 demonstrates the relevance of MAMP-triggered immunity in resistance to necrotrophic fungi.
Sujet(s)
Protéines d'Arabidopsis/immunologie , Arabidopsis/immunologie , Ascomycota/pathogénicité , Maladies des plantes/génétique , Immunité des plantes , Protein-Serine-Threonine Kinases/immunologie , Arabidopsis/génétique , Arabidopsis/microbiologie , Protéines d'Arabidopsis/génétique , Résistance à la maladie/génétique , Protéines fongiques/métabolisme , Données de séquences moléculaires , Maladies des plantes/microbiologie , Végétaux génétiquement modifiés/génétique , Végétaux génétiquement modifiés/immunologie , Végétaux génétiquement modifiés/microbiologie , Protein-Serine-Threonine Kinases/génétiqueRÉSUMÉ
Pathogens target important components of host immunity to cause disease. The Pseudomonas syringae type III-secreted effector HopU1 is a mono-ADP-ribosyltransferase required for full virulence on Arabidopsis thaliana. HopU1 targets several RNA-binding proteins including GRP7, whose role in immunity is still unclear. Here, we show that GRP7 associates with translational components, as well as with the pattern recognition receptors FLS2 and EFR. Moreover, GRP7 binds specifically FLS2 and EFR transcripts in vivo through its RNA recognition motif. HopU1 does not affect the protein-protein associations between GRP7, FLS2 and translational components. Instead, HopU1 blocks the interaction between GRP7 and FLS2 and EFR transcripts in vivo. This inhibition correlates with reduced FLS2 protein levels upon Pseudomonas infection in a HopU1-dependent manner. Our results reveal a novel virulence strategy used by a microbial effector to interfere with host immunity.
Sujet(s)
ADP ribose transferases/métabolisme , Protéines d'Arabidopsis/métabolisme , Arabidopsis/microbiologie , Maladies des plantes/immunologie , Protein kinases/métabolisme , Pseudomonas syringae/immunologie , ARN messager/génétique , Protéines de liaison à l'ARN/métabolisme , Récepteurs de reconnaissance de motifs moléculaires/métabolisme , Virulence/immunologie , ADP ribose transferases/génétique , Arabidopsis/immunologie , Arabidopsis/métabolisme , Protéines d'Arabidopsis/génétique , Protéines bactériennes/génétique , Protéines bactériennes/métabolisme , Technique de Western , Membrane cellulaire , Test de retard de migration électrophorétique , Immunité innée , Immunoprécipitation , Maladies des plantes/génétique , Maladies des plantes/microbiologie , Biosynthèse des protéines , Protein kinases/génétique , Pseudomonas syringae/génétique , Pseudomonas syringae/pathogénicité , ARN des plantes/génétique , Protéines de liaison à l'ARN/génétique , Réaction de polymérisation en chaine en temps réel , Récepteurs de reconnaissance de motifs moléculaires/génétique , RT-PCRRÉSUMÉ
The molecular mechanisms underlying the trade-off between plant innate immunity and steroid-mediated growth are controversial. Here, we report that activation of the transcription factor BZR1 is required and sufficient for suppression of immune signaling by brassinosteroids (BR). BZR1 induces the expression of several WRKY transcription factors that negatively control early immune responses. In addition, BZR1 associates with WRKY40 to mediate the antagonism between BR and immune signaling. We reveal that BZR1-mediated inhibition of immunity is particularly relevant when plant fast growth is required, such as during etiolation. Thus, BZR1 acts as an important regulator mediating the trade-off between growth and immunity upon integration of environmental cues. DOI: http://dx.doi.org/10.7554/eLife.00983.001.
Sujet(s)
Protéines d'Arabidopsis/physiologie , Arabidopsis/croissance et développement , Arabidopsis/immunologie , Protéines nucléaires/physiologie , Arabidopsis/génétique , Protéines de liaison à l'ADN , Gènes de planteRÉSUMÉ
Perception of pathogen-associated molecular patterns (PAMPs), such as bacterial flagellin (or the peptide flg22), by surface-localized receptors activates defense responses and subsequent immunity. In a previous forward-genetic screen aimed at the identification of Arabidopsis (Arabidopsis thaliana) flagellin-insensitive (fin) mutants, we isolated fin4, which is severely affected in flg22-triggered reactive oxygen species (ROS) bursts. Here, we report that FIN4 encodes the chloroplastic enzyme ASPARTATE OXIDASE (AO), which catalyzes the first irreversible step in the de novo biosynthesis of NAD. Genetic studies on the role of NAD have been hindered so far by the lethality of null mutants in NAD biosynthetic enzymes. Using newly identified knockdown fin alleles, we found that AO is required for the ROS burst mediated by the NADPH oxidase RBOHD triggered by the perception of several unrelated PAMPs. AO is also required for RBOHD-dependent stomatal closure. However, full AO activity is not required for flg22-induced responses that are RBOHD independent. Interestingly, although the fin4 mutation dramatically affects RBOHD function, it does not affect functions carried out by other members of the RBOH family, such as RBOHC and RBOHF. Finally, we determined that AO is required for stomatal immunity against the bacterium Pseudomonas syringae. Altogether, our work reveals a novel specific requirement for AO activity in PAMP-triggered RBOHD-dependent ROS burst and stomatal immunity. In addition, the availability of viable mutants for the chloroplastic enzyme AO will enable future detailed studies on the role of NAD metabolism in different cellular processes, including immunity, in Arabidopsis.
Sujet(s)
Amino-acid oxidoreductases/métabolisme , Protéines d'Arabidopsis/métabolisme , Arabidopsis/enzymologie , Arabidopsis/immunologie , Immunité des plantes/immunologie , Stomates de plante/immunologie , Acide abscissique/pharmacologie , Arabidopsis/microbiologie , Arabidopsis/physiologie , Chitine/pharmacologie , Flagelline/pharmacologie , Gènes de plante/génétique , Mutagenèse par insertion/génétique , Mutation/génétique , NADPH oxidase/métabolisme , Acide nicotinique/pharmacologie , Racines de plante/effets des médicaments et des substances chimiques , Racines de plante/enzymologie , Racines de plante/croissance et développement , Stomates de plante/effets des médicaments et des substances chimiques , Stomates de plante/microbiologie , Stomates de plante/physiologie , Pseudomonas syringae/effets des médicaments et des substances chimiques , Pseudomonas syringae/physiologie , Espèces réactives de l'oxygène/métabolisme , Récepteurs de reconnaissance de motifs moléculaires/métabolismeRÉSUMÉ
Plants and animals use innate immunity as a first defense against pathogens, a costly yet necessary tradeoff between growth and immunity. In Arabidopsis, the regulatory leucine-rich repeat receptor-like kinase (LRR-RLK) BAK1 combines with the LRR-RLKs FLS2 and EFR in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and the LRR-RLK BRI1 in brassinosteroid (BR)-mediated growth. Therefore, a potential tradeoff between these pathways mediated by BAK1 is often postulated. Here, we show a unidirectional inhibition of FLS2-mediated immune signaling by BR perception. Unexpectedly, this effect occurred downstream or independently of complex formation with BAK1 and associated downstream phosphorylation. Thus, BAK1 is not rate-limiting in these pathways. BRs also inhibited signaling triggered by the BAK1-independent recognition of the fungal PAMP chitin. Our results suggest a general mechanism operative in plants in which BR-mediated growth directly antagonizes innate immune signaling.
Sujet(s)
Arabidopsis/immunologie , Arabidopsis/microbiologie , Brassinostéroïdes/pharmacologie , Immunité des plantes/effets des médicaments et des substances chimiques , Pseudomonas/métabolisme , Récepteurs de reconnaissance de motifs moléculaires/métabolisme , Transduction du signal/effets des médicaments et des substances chimiques , Arabidopsis/effets des médicaments et des substances chimiques , Arabidopsis/enzymologie , Protéines d'Arabidopsis/métabolisme , Activation enzymatique/effets des médicaments et des substances chimiques , Flagelline/pharmacologie , Immunité des plantes/immunologie , Protein kinases/métabolisme , Protein-Serine-Threonine Kinases/métabolisme , Pseudomonas/effets des médicaments et des substances chimiques , Transduction du signal/immunologie , Stéroïdes hétérocycliques/pharmacologieRÉSUMÉ
In plant innate immunity, the leucine-rich repeat receptor kinase FLS2 recognizes the bacterial pathogen-associated molecular pattern (PAMP) flagellin. The molecular mechanisms underlying PAMP perception are not fully understood. Here, we reveal that the gaseous phytohormone ethylene is an integral part of PAMP-triggered immunity. Plants mutated in the key ethylene-signaling protein EIN2 are impaired in all FLS2-mediated responses, correlating with reduced FLS2 transcription and protein accumulation. The EIN3 and EIN3-like transcription factors, which depend on EIN2 activity for their accumulation, directly control FLS2 expression. Our results reveal a direct role for ethylene in regulation of an innate immune receptor.
Sujet(s)
Protéines d'Arabidopsis/génétique , Régulation de l'expression des gènes végétaux , Protéines nucléaires/génétique , Protein kinases/génétique , Récepteurs immunologiques/génétique , Facteurs de transcription/génétique , Arabidopsis/génétique , Arabidopsis/immunologie , Protéines d'Arabidopsis/biosynthèse , Protéines de liaison à l'ADN , Éthylènes/pharmacologie , Immunité innée/effets des médicaments et des substances chimiques , Protein kinases/biosynthèse , Récepteurs immunologiques/biosynthèseRÉSUMÉ
BACKGROUND: Plant non-specific lipid transfer proteins (nsLTPs) are encoded by multigene families and possess physiological functions that remain unclear. Our objective was to characterize the complete nsLtp gene family in rice and arabidopsis and to perform wheat EST database mining for nsLtp gene discovery. RESULTS: In this study, we carried out a genome-wide analysis of nsLtp gene families in Oryza sativa and Arabidopsis thaliana and identified 52 rice nsLtp genes and 49 arabidopsis nsLtp genes. Here we present a complete overview of the genes and deduced protein features. Tandem duplication repeats, which represent 26 out of the 52 rice nsLtp genes and 18 out of the 49 arabidopsis nsLtp genes identified, support the complexity of the nsLtp gene families in these species. Phylogenetic analysis revealed that rice and arabidopsis nsLTPs are clustered in nine different clades. In addition, we performed comparative analysis of rice nsLtp genes and wheat (Triticum aestivum) EST sequences indexed in the UniGene database. We identified 156 putative wheat nsLtp genes, among which 91 were found in the 'Chinese Spring' cultivar. The 122 wheat non-redundant nsLTPs were organized in eight types and 33 subfamilies. Based on the observation that seven of these clades were present in arabidopsis, rice and wheat, we conclude that the major functional diversification within the nsLTP family predated the monocot/dicot divergence. In contrast, there is no type VII nsLTPs in arabidopsis and type IX nsLTPs were only identified in arabidopsis. The reason for the larger number of nsLtp genes in wheat may simply be due to the hexaploid state of wheat but may also reflect extensive duplication of gene clusters as observed on rice chromosomes 11 and 12 and arabidopsis chromosome 5. CONCLUSION: Our current study provides fundamental information on the organization of the rice, arabidopsis and wheat nsLtp gene families. The multiplicity of nsLTP types provide new insights on arabidopsis, rice and wheat nsLtp gene families and will strongly support further transcript profiling or functional analyses of nsLtp genes. Until such time as specific physiological functions are defined, it seems relevant to categorize plant nsLTPs on the basis of sequence similarity and/or phylogenetic clustering.
Sujet(s)
Arabidopsis/génétique , Protéines de transport/génétique , Oryza/génétique , Protéines végétales/génétique , Triticum/génétique , Séquence d'acides aminés , Protéines d'Arabidopsis/génétique , Bases de données génétiques , Étiquettes de séquences exprimées , Gènes de plante , Variation génétique , Génome végétal , Génomique , Données de séquences moléculaires , Famille multigénique , Phylogenèse , Similitude de séquences d'acides aminés , Spécificité d'espèce , Séquences répétées en tandemRÉSUMÉ
Plant non-specific lipid transfer proteins (nsLTPs) are encoded by a multigene family and support physiological functions, which remain unclear. We adapted an efficient ligation-mediated polymerase chain reaction (LM-PCR) procedure that enabled isolation of 22 novel Triticum aestivum nsLtp (TaLtp) genes encoding types 1 and 2 nsLTPs. A phylogenetic tree clustered the wheat nsLTPs into ten subfamilies comprising 1-7 members. We also studied the activity of four type 1 and two type 2 TaLtp gene promoters in transgenic rice using the 1-Glucuronidase reporter gene. The activities of the six promoters displayed both overlapping and distinct features in rice. In vegetative organs, these promoters were active in leaves and root vascular tissues while no beta-Glucuronidase (GUS) activity was detected in stems. In flowers, the GUS activity driven by the TaLtp7.2a, TaLtp9.1a, TaLtp9.2d, and TaLtp9.3e gene promoters was associated with vascular tissues in glumes and in the extremities of anther filaments whereas only the TaLtp9.4a gene promoter was active in anther epidermal cells. In developing grains, GUS activity and GUS immunolocalization data evidenced complex patterns of activity of the TaLtp7.1a, TaLtp9.2d, and TaLtp9.4a gene promoters in embryo scutellum and in the grain epicarp cell layer. In contrast, GUS activity driven by TaLtp7.2a, TaLtp9.1a, and TaLtp9.3e promoters was restricted to the vascular bundle of the embryo scutellum. This diversity of TaLtp gene promoter activity supports the hypothesis that the encoded TaLTPs possess distinct functions in planta.
Sujet(s)
Protéines de transport/métabolisme , Oryza/métabolisme , Régions promotrices (génétique) , Triticum/génétique , Séquence d'acides aminés , Protéines de transport/génétique , Fleurs/métabolisme , Fluorimétrie , Gènes rapporteurs , Glucuronidase/métabolisme , Immunohistochimie , Données de séquences moléculaires , Famille multigénique , Oryza/génétique , Oryza/croissance et développement , Racines de plante/métabolisme , Végétaux génétiquement modifiés/métabolisme , Graines/croissance et développement , Graines/métabolisme , Analyse de séquence d'ADN , Similitude de séquences d'acides aminés , Transformation génétiqueRÉSUMÉ
Nine cDNA clones encoding non-specific lipid transfer proteins (nsLTPs) were isolated from Triticum aestivum and Triticum durum cDNA libraries and characterized. One cDNA is predicted to encode a type 2 nsLTP (7 kDa) while others encode type 1 nsLTPs (9 kDa). All encoded proteins contain an N-terminal signal sequence and possess the characteristic features of nsLTPs. The genomic structures of the wheat nsLtp genes show that type 2 TaLtp7.1a, TaLtp7.2a and type 1 TaLtp9.2b genes lack introns while the other type 1 genes consist of one intron. Construction of a phylogenic tree of Poaceae nsLTPs shows that wheat nsLTPs can be divided into eleven distinct groups and are closely related to barley sequences. Using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, the expression patterns of nine nsLtp genes were studied during wheat seed development and germination. We identified three different profiles of nsLtp gene transcript accumulation. Whereas TdLtp7.1a, TdLtp9.4a and TdLtp9.7a transcripts were detected during all maturation stages, TdLtp7.2a, TdLtp9.2a, TdLtp9.3a, TdLtp9.5a and TdLtp9.6a transcripts were only present in the first and TdLtp9.1a in the last stages of seed development. Moreover, these nine wheat nsLtp genes are not seed-specific and are also expressed in the coleoptile of young seedlings. The present study revealed the complexity of the wheat nsLtp gene family and showed that the expression of nsLtp genes is developmentally regulated in the seeds, suggesting a specific function for each of the corresponding proteins.