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Int J Syst Evol Microbiol ; 69(7): 2023-2027, 2019 Jul.
Article de Anglais | MEDLINE | ID: mdl-31066656

RÉSUMÉ

A Gram-stain-negative, rod-shaped strain isolated from pig-production environments was identified as a new species within the genus Yersinia using multifaceted genomic and biochemical approaches. The genome of this strain was closed using a hybrid assembly approach combining both high accuracy short read sequencing data with long read sequencing technology. Phylogenetic analysis of the 16S rRNA gene showed ~98 % similarity to Yersinia kristensenii and ~98 % similarity to Yersinia enterocolitica. Average nucleotide identity (OrthoANI) values were calculated as 85.79 % to Y. kristensenii ATCC 33638T and 85.73 % to Y. enterocolitica ATCC 9610T thereby providing evidence that this isolate should be considered as a novel species. The type strain is CFS1934T (=NCTC 14222T=LMG 31076T).


Sujet(s)
Phylogenèse , Suidae/microbiologie , Yersinia/classification , Animaux , Techniques de typage bactérien , Composition en bases nucléiques , ADN bactérien/génétique , Irlande , Tonsille palatine/microbiologie , ARN ribosomique 16S/génétique , Analyse de séquence d'ADN , Yersinia/isolement et purification
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