[Screening of Germination and Seedling Conditions of Trollius chinensis Seeds from Zhangjiakou].

Objective: To screen the germination and seedling conditions of Trollius chinensis seeds from Chongli county, Zhangjiakou city. To provide the theoretical basis and practical technology for establishing technology system of seedling and cultivation of Trollius chinensis seeds in Zhangjiakou city. Methods: The data were collected and analyzed by the contrast test of greenhouse. Results: The most suitable conditions of Trollius chinensis seedling treatment were as follows, the concentration of GA3 was 800 mg / m L at 4 ℃,and under this condition in infusing for 4 days, and placed in the artificial climate chamber, the temperature was 20 ℃,light treatment for 4 days with 12 h light and 12 h darkness, and sprayed PEG 6000( 30 g / L). Trollius chinesis seeds were sowed on the 4th day, peat soil was selected as matrix, and the germination rate reached more than 85%. Conclusion: According to the characteristics of the seedling appearance and the conditions of the seedlings after transplanting, the seedling growth is robust and the survival rate is much higher.

[Spectroscopic study on binding of indomethacin to bovine serum albumin].

The interaction between indomethacin (IM) and bovine serum albumin (BSA) under physiological condition was studied using fluorescence and absorption spectra. It was proved that fluorescence quenching of BSA by IM is deduced by combining static quenching with nonradiative energy transfer. The binding constants were obtained by fluorescence quenching method. The main sorts of binding force were determined according to the thermodynamic parameters. The binding distances and energy transfer efficiencies between indomethacin and bovine serum albumin were obtained based on the theory of Forster nonradiative energy transfer. Combining the results of UV absorption and fluorescence spectroscopic methods, the binding mode of IM with BSA was discussed.