RÉSUMÉ
Digital Breast Tomosynthesis (DBT) is a modern 3D Computed Tomography X-ray technique for the early detection of breast tumors, which is receiving growing interest in the medical and scientific community. Since DBT performs incomplete sampling of data, the image reconstruction approaches based on iterative methods are preferable to the classical analytic techniques, such as the Filtered Back Projection algorithm, providing fewer artifacts. In this work, we consider a Model-Based Iterative Reconstruction (MBIR) method well suited to describe the DBT data acquisition process and to include prior information on the reconstructed image. We propose a gradient-based solver named Scaled Gradient Projection (SGP) for the solution of the constrained optimization problem arising in the considered MBIR method. Even if the SGP algorithm exhibits fast convergence, the time required on a serial computer for the reconstruction of a real DBT data set is too long for the clinical needs. In this paper we propose a parallel SGP version designed to perform the most expensive computations of each iteration on Graphics Processing Unit (GPU). We apply the proposed parallel approach on three different GPU boards, with computational performance comparable with that of the boards usually installed in commercial DBT systems. The numerical results show that the proposed GPU-based MBIR method provides accurate reconstructions in a time suitable for clinical trials.
Sujet(s)
Algorithmes , Tumeurs du sein/imagerie diagnostique , Tumeurs du sein/anatomopathologie , Infographie , Mammographie/méthodes , Modèles théoriques , Interprétation d'images radiographiques assistée par ordinateur/méthodes , Femelle , Humains , Imagerie tridimensionnelle/méthodes , Fantômes en imagerie , Amélioration d'image radiographique/méthodes , Tomodensitométrie/méthodesRÉSUMÉ
Biofilms enhance rates of gene exchange, access to specific nutrients, and cell survivability. Haloarchaea in Deep Lake, Antarctica, are characterized by high rates of intergenera gene exchange, metabolic specialization that promotes niche adaptation, and are exposed to high levels of UV-irradiation in summer. Halorubrum lacusprofundi from Deep Lake has previously been reported to form biofilms. Here we defined growth conditions that promoted the formation of biofilms and used microscopy and enzymatic digestion of extracellular material to characterize biofilm structures. Extracellular DNA was found to be critical to biofilms, with cell surface proteins and quorum sensing also implicated in biofilm formation. Quantitative proteomics was used to define pathways and cellular processes involved in forming biofilms; these included enhanced purine synthesis and specific cell surface proteins involved in DNA metabolism; post-translational modification of cell surface proteins; specific pathways of carbon metabolism involving acetyl-CoA; and specific responses to oxidative stress. The study provides a new level of understanding about the molecular mechanisms involved in biofilm formation of this important member of the Deep Lake community.
Sujet(s)
Biofilms , Halorubrum/métabolisme , Halorubrum/physiologie , Protéomique/méthodes , Régions antarctiques , Biofilms/croissance et développement , Deoxyribonuclease I/métabolisme , Endopeptidase K/métabolisme , Halorubrum/cytologie , Halorubrum/ultrastructure , Voies et réseaux métaboliques , Microscopie de fluorescence , Plancton/métabolisme , Détection du quorumRÉSUMÉ
No systems have been reported for genetic manipulation of cold-adapted Archaea. Halorubrum lacusprofundi is an important member of Deep Lake, Antarctica (~10% of the population), and is amendable to laboratory cultivation. Here we report the development of a shuttle-vector and targeted gene-knockout system for this species. To investigate the function of acetamidase/formamidase genes, a class of genes not experimentally studied in Archaea, the acetamidase gene, amd3, was disrupted. The wild-type grew on acetamide as a sole source of carbon and nitrogen, but the mutant did not. Acetamidase/formamidase genes were found to form three distinct clades within a broad distribution of Archaea and Bacteria. Genes were present within lineages characterized by aerobic growth in low nutrient environments (e.g. haloarchaea, Starkeya) but absent from lineages containing anaerobes or facultative anaerobes (e.g. methanogens, Epsilonproteobacteria) or parasites of animals and plants (e.g. Chlamydiae). While acetamide is not a well characterized natural substrate, the build-up of plastic pollutants in the environment provides a potential source of introduced acetamide. In view of the extent and pattern of distribution of acetamidase/formamidase sequences within Archaea and Bacteria, we speculate that acetamide from plastics may promote the selection of amd/fmd genes in an increasing number of environmental microorganisms.
Sujet(s)
Amidohydrolases/génétique , Protéines d'archée/génétique , Régulation de l'expression des gènes archéens , Vecteurs génétiques/composition chimique , Halorubrum/génétique , Amidohydrolases/déficit , Régions antarctiques , Protéines d'archée/métabolisme , Dépollution biologique de l'environnement , Milieux de culture/composition chimique , Milieux de culture/pharmacologie , Délétion de gène , Génie génétique , Vecteurs génétiques/métabolisme , Halorubrum/classification , Halorubrum/effets des médicaments et des substances chimiques , Halorubrum/enzymologie , Humains , Phylogenèse , Matières plastiques/métabolisme , Cartographie de restriction , Transformation génétique , Polluants chimiques de l'eau/métabolismeRÉSUMÉ
Cold environments dominate the Earth's biosphere and the resident microorganisms play critical roles in fulfilling global biogeochemical cycles. However, only few studies have examined the molecular basis of thermosensing; an ability that microorganisms must possess in order to respond to environmental temperature and regulate cellular processes. Two component regulatory systems have been inferred to function in thermal regulation of gene expression, but biochemical studies assessing these systems in Bacteria are rare, and none have been performed in Archaea or psychrophiles. Here we examined the LtrK/LtrR two component regulatory system from the Antarctic archaeon, Methanococcoides burtonii, assessing kinase and phosphatase activities of wild-type and mutant proteins. LtrK was thermally unstable and had optimal phosphorylation activity at 10 °C (the lowest optimum activity for any psychrophilic enzyme), high activity at 0 °C and was rapidly thermally inactivated at 30 °C. These biochemical properties match well with normal environmental temperatures of M. burtonii (0-4 °C) and the temperature this psychrophile is capable of growing at in the laboratory (-2 to 28 °C). Our findings are consistent with a role for LtrK in performing phosphotransfer reactions with LtrR that could lead to temperature-dependent gene regulation.
Sujet(s)
Adaptation physiologique/génétique , Protéines d'archée/génétique , Basse température , Methanosarcinaceae/génétique , Séquence d'acides aminés , Régions antarctiques , Protéines d'archée/composition chimique , Protéines d'archée/métabolisme , Calorimétrie différentielle à balayage , Clonage moléculaire , Simulation numérique , Électrophorèse sur gel de polyacrylamide , Régulation de l'expression des gènes archéens , Methanosarcinaceae/métabolisme , Modèles moléculaires , Mutation , Phosphoric monoester hydrolases/composition chimique , Phosphoric monoester hydrolases/génétique , Phosphoric monoester hydrolases/métabolisme , Phosphorylation , Phosphotransferases/composition chimique , Phosphotransferases/génétique , Phosphotransferases/métabolisme , Domaines protéiques , Stabilité protéique , Similitude de séquences d'acides aminésRÉSUMÉ
Over recent years threats to racing have expanded to include naturally occurring biological molecules, such as peptides and proteins, and their synthetic analogues. Traditionally, antibodies have been used to enable detection of these compounds as they allow purification and concentration of the analyte of interest. The rapid expansion of peptide-based therapeutics necessitates a similarly rapid development of suitable antibodies or other means of enrichment. Potential alternative enrichment strategies include the use of aptamers, which offer the significant advantage of chemical synthesis once the nucleic acid sequence is known. A method was developed for the enrichment, detection and quantitation of gonadotropin-releasing hormone (GnRH) in equine urine using aptamer-based enrichment and LC-MS/MS. The method achieved comparable limits of detection (1 pg/mL) and quantification (2.5 pg/mL) to previously published antibody-based enrichment methods. The intra- and inter-assay precision achieved was less than 10% at both 5 and 20 pg/mL, and displayed a working dynamic range of 2.5-100 pg/mL. Significant matrix enhancement (170 ± 8%) and low analytical recovery (29 ± 15%) was observed, although the use of an isotopically heavy labelled GnRH peptide, GnRH (Pro(13)C5,(15)N), as the internal standard provides compensation for these parameters. Within the current limits of detection GnRH was detectable up to 1h post administration in urine and identification of a urinary catabolite extended this detection window to 4h. Based on the results of this preliminary investigation we propose the use of aptamers as a viable alternative to antibodies in the enrichment of peptide targets from equine urine.
Sujet(s)
Aptamères nucléotidiques/composition chimique , Chromatographie en phase liquide/méthodes , Dopage sportif/prévention et contrôle , Hormone de libération des gonadotrophines/analyse , Equus caballus/urine , Fragments peptidiques/composition chimique , Spectrométrie de masse en tandem/méthodes , Animaux , Anticorps/composition chimique , Hormone de libération des gonadotrophines/composition chimique , Hormone de libération des gonadotrophines/isolement et purificationRÉSUMÉ
TRAM domain proteins present in Archaea and Bacteria have a ß-barrel shape with anti-parallel ß-sheets that form a nucleic acid binding surface; a structure also present in cold shock proteins (Csps). Aside from protein structures, experimental data defining the function of TRAM domains is lacking. Here, we explore the possible functional properties of a single TRAM domain protein, Ctr3 (cold-responsive TRAM domain protein 3) from the Antarctic archaeon Methanococcoides burtonii that has increased abundance during low temperature growth. Ribonucleic acid (RNA) bound by Ctr3 in vitro was determined using RNA-seq. Ctr3-bound M. burtoniiâ RNA with a preference for transfer (t)RNA and 5S ribosomal RNA, and a potential binding motif was identified. In tRNA, the motif represented the C loop; a region that is conserved in tRNA from all domains of life and appears to be solvent exposed, potentially providing access for Ctr3 to bind. Ctr3 and Csps are structurally similar and are both inferred to function in low temperature translation. The broad representation of single TRAM domain proteins within Archaea compared with their apparent absence in Bacteria, and scarcity of Csps in Archaea but prevalence in Bacteria, suggests they represent distinct evolutionary lineages of functionally equivalent RNA-binding proteins.
Sujet(s)
Protéines d'archée/composition chimique , Methanosarcinaceae/génétique , ARN des archées/composition chimique , Protéines de liaison à l'ARN/composition chimique , Régions antarctiques , Protéines d'archée/génétique , Protéines d'archée/métabolisme , Basse température , ARN des archées/métabolisme , ARN ribosomique 5S/composition chimique , ARN ribosomique 5S/métabolisme , ARN de transfert/composition chimique , ARN de transfert/métabolisme , Protéines de liaison à l'ARN/génétique , Protéines de liaison à l'ARN/métabolismeRÉSUMÉ
Although deconvolution can improve the quality of any type of microscope, the high computational time required has so far limited its massive spreading. Here we demonstrate the ability of the scaled-gradient-projection (SGP) method to provide accelerated versions of the most used algorithms in microscopy. To achieve further increases in efficiency, we also consider implementations on graphic processing units (GPUs). We test the proposed algorithms both on synthetic and real data of confocal and STED microscopy. Combining the SGP method with the GPU implementation we achieve a speed-up factor from about a factor 25 to 690 (with respect the conventional algorithm). The excellent results obtained on STED microscopy images demonstrate the synergy between super-resolution techniques and image-deconvolution. Further, the real-time processing allows conserving one of the most important property of STED microscopy, i.e the ability to provide fast sub-diffraction resolution recordings.
Sujet(s)
Infographie/instrumentation , Amélioration d'image/instrumentation , Amélioration d'image/méthodes , Microscopie confocale/instrumentation , Microscopie confocale/méthodes , Microscopie de fluorescence/instrumentation , Microscopie de fluorescence/méthodes , Algorithmes , Systèmes informatiques , Conception d'appareillage , Analyse de panne d'appareillage , Traitement du signal assisté par ordinateur/instrumentationRÉSUMÉ
The bulk of the Earth's biosphere is cold (e.g. 90% of the ocean's waters are ≤ 5°C), sustaining a broad diversity of microbial life. The permanently cold environments vary from the deep ocean to alpine reaches and to polar regions. Commensurate with the extent and diversity of the ecosystems that harbour psychrophilic life, the functional capacity of the microorganisms that inhabitat the cold biosphere are equally diverse. As a result, indigenous psychrophilic microorganisms provide an enormous natural resource of enzymes that function effectively in the cold, and these cold-adapted enzymes have been targeted for their biotechnological potential. In this review we describe the main properties of enzymes from psychrophiles and describe some of their known biotechnological applications and ways to potentially improve their value for biotechnology. The review also covers the use of metagenomics for enzyme screening, the development of psychrophilic gene expression systems and the use of enzymes for cleaning.
Sujet(s)
Biotechnologie/méthodes , Microbiologie de l'environnement , Enzymes/métabolisme , Enzymes/effets des radiations , Basse température , Enzymes/isolement et purificationRÉSUMÉ
Methanococcoides burtonii is a member of the Archaea that was isolated from Ace Lake in Antarctica and is a valuable model for studying cold adaptation. Low temperature transcriptional regulation of global gene expression, and the arrangement of transcriptional units in cold-adapted archaea has not been studied. We developed a microarray for determining which genes are expressed in operons, and which are differentially expressed at low (4°C) or high (23°C) temperature. Approximately 55% of genes were found to be arranged in operons that range in length from 2 to 23 genes, and mRNA abundance tended to increase with operon length. Analysing microarray data previously obtained by others for Halobacterium salinarum revealed a similar correlation between operon length and mRNA abundance, suggesting that operons may play a similar role more broadly in the Archaea. More than 500 genes were differentially expressed at levels up to ≈ 24-fold. A notable feature was the upregulation of genes involved in maintaining RNA in a state suitable for translation in the cold. Comparison between microarray experiments and results previously obtained using proteomics indicates that transcriptional regulation (rather than translation) is primarily responsible for controlling gene expression in M. burtonii. In addition, certain genes (e.g. involved in ribosome structure and methanogenesis) appear to be regulated post-transcriptionally. This is one of few experimental studies describing the genome-wide distribution and regulation of operons in archaea.
Sujet(s)
Régulation de l'expression des gènes archéens , Methanosarcinaceae/génétique , Methanosarcinaceae/métabolisme , Température , Adaptation physiologique/génétique , Régions antarctiques , Protéines d'archée/génétique , Analyse de profil d'expression de gènes , Opéron , ProtéomiqueRÉSUMÉ
The adaptive response of the marine bacterium Sphingopyxis alaskensis RB2256 to solar radiation (both visible and ultraviolet) was assessed by a quantitative proteomic approach using iTRAQ (isobaric tags for relative and absolute quantification). Both growth phase (mid-log and stationary phase) and duration (80 min or 8 h) of different light treatments (combinations of visible light, UV-A and UV-B) were assessed relative to cultures maintained in the dark. Rates of total protein synthesis and viability were also assessed. Integrating knowledge from the physiological experiments with quantitative proteomics of the 12 conditions tested provided unique insight into the adaptation biology of UV and visible light responses of S. alaskensis. High confidence identifications were obtained for 811 proteins (27% of the genome), 119 of which displayed significant quantitative differences. Mid-log-phase cultures produced twice as many proteomic changes as stationary-phase cultures, while extending the duration of irradiation exposure of stationary-phase cultures did not increase the total number of quantitative changes. Proteins with significant quantitative differences were identified that were characteristic of growth phase and light treatment, and cellular processes, pathways and interaction networks were determined. Key factors of the solar radiation adaptive response included DNA-binding proteins implicated in reducing DNA damage, detoxification of toxic compounds such as glyoxal and reactive oxygen species, iron sequestration to minimize oxidative stress, chaperones to control protein re/folding, alterations to nitrogen metabolism, and specific changes to transcriptional and translational processes.
Sujet(s)
Protéome/effets des radiations , Sphingomonadaceae , Lumière du soleil , Protéines bactériennes/composition chimique , Protéines bactériennes/effets des radiations , Régulation de l'expression des gènes bactériens/effets des radiations , Viabilité microbienne/effets des radiations , Biosynthèse des protéines/effets des radiations , Protéomique , Eau de mer/microbiologie , Sphingomonadaceae/physiologie , Sphingomonadaceae/effets des radiations , Spectrométrie de masse en tandem , Rayons ultraviolets , Microbiologie de l'eauRÉSUMÉ
The activity-stability-structure relationship of the cold-active alkaline phosphatase from Red Arctic shrimp, Pandalus borealis (SAP) was studied by chemically modifying aliphatic (C-H) or amino (NH2) groups using benzophenone tetracarboxylic derivatives in either a light (UV-A) or dark reaction. The response of the cold-adapted enzyme was compared to a similarly modified calf alkaline phosphatase (CAP). MALDI-TOF-MS was used to determine the extent and nature of the modifications in both SAP and CAP. On average 2 to 4 amino acid residues were linked to a BP-modifier, with up to 18 to 21 amino acids modified in a smaller portion of the material. The effect of the modifications on kinetic and thermodynamic properties varied with the enzyme and type of modification. The aliphatic-group modified SAP demonstrated typical characteristics of a mesophilic enzyme, consistent with an activity-stability trade-off where gain in thermostability was attained at the expense of decreased activity. In contrast, the activity of the amino-group modified SAP attained an even more psychrophilic character with respect to its kinetic (increase in kcat and Km) and thermodynamic (reduction in deltaH#) properties. Interestingly, the amino-group modified SAP also acquired higher thermostability, thus demonstrating that both activity and stability can be simultaneously enhanced using chemical modification. The study demonstrates the applicability of benzophenone chemical modification for improving the thermal properties of enzymes from psychrophiles and mesophiles.
Sujet(s)
Phosphatase alcaline/métabolisme , Benzophénones/composition chimique , Phosphatase alcaline/composition chimique , Bactéries/enzymologie , Benzophénones/métabolisme , Stabilité enzymatique/physiologie , Cinétique , Spectrométrie de masse MALDI , TempératureRÉSUMÉ
The oceans of the world are nutrient-limited environments that support a dynamic diversity of microbial life. Heterotrophic prokaryotes proliferate in oligotrophic regions and affect nutrient transformation and remineralization thereby impacting directly on the all marine biota. An important challenge in studying the microbial ecology of oligotrophic environments has been the isolation of ecologically important species. This goal has been recognized not only for its relevance in defining the dynamics of community composition, but for enabling physiological studies of competitive species and inferring their impact on the microbial food web. This review describes the successful isolation attempts of the ultramicrobacterium, Sphingopyxis alaskensis (formerly described as Sphingomonas alaskensis) using extinction dilution culturing methods. It then provides a comprehensive perspective of the unique physiological and genetic properties that have been identified that distinguish it from typical copiotrophic species. These properties are described through studies of the growth phase and growth rate control of macromolecular synthesis, stress resistance and global gene expression (proteomics). We also discuss the importance of integrating ecological and physiological approaches for studying microorganisms in marine environments.
Sujet(s)
Écosystème , Sphingomonas/physiologie , Microbiologie de l'eau , Alaska , ADN bactérien/génétique , Mer du Nord , Océan Pacifique , Sphingomonas/génétique , Sphingomonas/ultrastructureRÉSUMÉ
Numerous studies have established the importance of picoplankton (microorganisms of < or =2 microm in length) in energy flow and nutrient cycling in marine oligotrophic environments, and significant effort has been directed at identifying and isolating heterotrophic picoplankton from the world's oceans. Using a method of diluting natural seawater to extinction followed by monthly subculturing for 12 months, a bacterium was isolated that was able to form colonies on solid medium. The strain was isolated from a 10(5) dilution of seawater where the standing bacterial count was 3.1 x 10(5) cells ml(-1). This indicated that the isolate was representative of the most abundant bacteria at the sampling site, 1.5 km from Cape Muroto, Japan. The bacterium was characterized and found to be ultramicrosized (less than 0.1 microm(3)), and the size varied to only a small degree when the cells were starved or grown in rich media. A detailed molecular (16S rRNA sequence, DNA-DNA hybridization, G+C mol%, genome size), chemotaxonomic (lipid analysis, morphology), and physiological (resistance to hydrogen peroxide, heat, and ethanol) characterization of the bacterium revealed that it was a strain of Sphingomonas alaskensis. The type strain, RB2256, was previously isolated from Resurrection Bay, Alaska, and similar isolates have been obtained from the North Sea. The isolation of this species over an extended period, its high abundance at the time of sampling, and its geographical distribution indicate that it has the capacity to proliferate in ocean waters and is therefore likely to be an important contributor in terms of biomass and nutrient cycling in marine environments.
Sujet(s)
Eau de mer/microbiologie , Sphingomonas/classification , Sphingomonas/isolement et purification , Composition en bases nucléiques , Numération de colonies microbiennes , ADN bactérien/analyse , ADN bactérien/composition chimique , ADN bactérien/génétique , ADN ribosomique/analyse , ADN ribosomique/composition chimique , ADN ribosomique/génétique , Acides gras/analyse , Réaction de choc thermique , Données de séquences moléculaires , Hybridation d'acides nucléiques , Océan Pacifique , Phylogenèse , ARN ribosomique 16S/génétique , Analyse de séquence d'ADN , Sphingomonas/génétique , Sphingomonas/ultrastructureRÉSUMÉ
The marine oligotrophic ultramicrobacterium Sphingomonas alaskensis RB2256 has a physiology that is distinctly different from that of typical copiotrophic marine bacteria, such as Vibrio angustum S14. This includes a high level of inherent stress resistance and the absence of starvation-induced stress resistance to hydrogen peroxide. In addition to periods of starvation in the ocean, slow, nutrient-limited growth is likely to be encountered by oligotrophic bacteria for substantial periods of time. In this study we examined the effects of growth rate on the resistance of S. alaskensis RB2256 to hydrogen peroxide under carbon or nitrogen limitation conditions in nutrient-limited chemostats. Glucose-limited cultures of S. alaskensis RB2256 at a specific growth rate of 0.02 to 0.13 h(-1) exhibited 10,000-fold-greater viability following 60 min of exposure to 25 mM hydrogen peroxide than cells growing at a rate of 0.14 h(-1) or higher. Growth rate control of stress resistance was found to be specific to carbon and energy limitation in this organism. In contrast, V. angustum S14 did not exhibit growth rate-dependent stress resistance. The dramatic switch in stress resistance that was observed under carbon and energy limitation conditions has not been described previously in bacteria and thus may be a characteristic of the oligotrophic ultramicrobacterium. Catalase activity varied marginally and did not correlate with the growth rate, indicating that hydrogen peroxide breakdown was not the primary mechanism of resistance. More than 1,000 spots were resolved on silver-stained protein gels for cultures growing at rates of 0.026, 0.076, and 0.18 h(-1). Twelve protein spots had intensities that varied by more than twofold between growth rates and hence are likely to be important for growth rate-dependent stress resistance. These studies demonstrated the crucial role that nutrient limitation plays in the physiology of S. alaskensis RB2256, especially under oxidative stress conditions.
Sujet(s)
Peroxyde d'hydrogène/pharmacologie , Sphingomonas/effets des médicaments et des substances chimiques , Sphingomonas/croissance et développement , Catalase/métabolisme , Milieux de culture/composition chimique , Résistance microbienne aux médicaments , Électrophorèse bidimensionnelle sur gel , Régulation de l'expression des gènes bactériens , Glucose/métabolisme , Eau de mer/microbiologieRÉSUMÉ
Low-temperature-adapted archaea are abundant in the environment, yet little is known about the thermal adaptation of their proteins. We have previously compared elongation factor 2 (EF-2) proteins from Antarctic (Methanococcoides burtonii) and thermophilic (Methanosarcina thermophila) methanogens and found that the M. burtonii EF-2 had greater intrinsic activity at low temperatures and lower thermal stability at high temperatures (T. Thomas and R. Cavicchioli, J. Bacteriol. 182:1328-1332, 2000). While the gross thermal properties correlated with growth temperature, the activity and stability profiles of the EF-2 proteins did not precisely match the optimal growth temperature of each organism. This indicated that intracellular components may affect the thermal characteristics of the EF-2 proteins, and in this study we examined the effects of ribosomes and intracellular solutes. At a high growth temperature the thermophile produced high levels of potassium glutamate, which, when assayed in vitro with EF-2, retarded thermal unfolding and increased catalytic efficiency. In contrast, for the Antarctic methanogen adaptation to growth at a low temperature did not involve the accumulation of stabilizing organic solutes but appeared to result from an increased affinity of EF-2 for GTP and high levels of EF-2 in the cell relative to its low growth rate. Furthermore, ribosomes greatly stimulated GTPase activity and moderately stabilized both EF-2 proteins. These findings illustrate the different physiological strategies that have evolved in two phylogenetically related but thermally distinct methanogens to enable EF-2 to function satisfactorily.
Sujet(s)
Methanosarcina/métabolisme , Methanosarcinaceae/métabolisme , Facteur-2 d'élongation de la chaîne peptidique/métabolisme , Ribosomes/métabolisme , Technique de Western , Basse température , Test ELISA , dGTPases/métabolisme , Température élevée , Spectroscopie par résonance magnétique , Methanosarcina/croissance et développement , Methanosarcinaceae/croissance et développement , Facteur-2 d'élongation de la chaîne peptidique/composition chimiqueRÉSUMÉ
Sphingomonas sp. strain RB2256 is representative of the ultramicrobacteria that proliferate in oligotrophic marine waters. While this class of bacteria is well adapted for growth with low concentrations of nutrients, their ability to respond to complete nutrient deprivation has not previously been investigated. In this study, we examined two-dimensional protein profiles for logarithmic and stationary-phase cells and found that protein spot intensity was regulated by up to 70-fold. A total of 72 and 177 spots showed increased or decreased intensity, respectively, by at least twofold during starvation. The large number of protein spots (1,500) relative to the small genome size (ca. 1.5 Mb) indicates that gene expression may involve co- and posttranslational modifications of proteins. Rates of protein and RNA synthesis were examined throughout the growth phase and up to 7 days of starvation and revealed that synthesis was highly regulated. Rates of protein synthesis and cellular protein content were compared to ribosome content, demonstrating that ribosome synthesis was not directly linked to protein synthesis and that the function of ribosomes may not be limited to translation. By comparing the genetic capacity and physiological responses to starvation of RB2256 to those of the copiotrophic marine bacterium Vibrio angustum S14 (J. Ostling, L. Holmquist, and S. Kjelleberg, J. Bacteriol. 178:4901-4908, 1996), the characteristics of a distinct starvation response were defined for Sphingomonas strain RB2256. The capacity of this ultramicrobacterium to respond to starvation is discussed in terms of the ecological relevance of complete nutrient deprivation in an oligotrophic marine environment. These studies provide the first evidence that marine oligotrophic ultramicrobacteria may be expected to include a starvation response and the capacity for a high degree of gene regulation.
Sujet(s)
Sphingomonas/physiologie , Protéines bactériennes/biosynthèse , Protéines bactériennes/isolement et purification , Division cellulaire , Milieux de culture , Glucose/métabolisme , Cinétique , ARN bactérien/biosynthèse , Eau de mer/microbiologie , Sphingomonas/croissance et développementRÉSUMÉ
The first structural analysis of elongation factor G (EF-G) from a cold-adapted bacterium is presented. EF-G is an essential protein involved in the elongation process during protein synthesis and is therefore thought to play a crucial role in the low-temperature adaptation of cold-adapted microorganisms. To define its importance, the EF-G gene (fus) from the psychrotolerant bacterium Arthrobacter globiformis SI55 was cloned and sequenced. The deduced primary structure of the elongation factor is composed of 700 amino acids with a predicted molecular mass of 77.4 kDa. A three-dimensional model of the protein was constructed based on the known crystal structures of structurally homologous proteins. Structural features that might potentially be important for activity and flexibility at low temperature were deduced by comparisons with models of the EF-G proteins from the closely related mesophiles Micrococcus luteus and Mycobacterium tuberculosis. These features include a loss in the number of salt bridges in intradomain and interdomain positions, increased solvent interactions mediated by greater charge and polarity on domain surfaces, loop insertions, loss of proline residues in loop structures, and an increase of hydrophobicity in core regions. Specific changes have also been identified in the catalytic domain (G domain) and sites of potential ribosome interaction, which may directly affect guanosine triphosphate (GTP) hydrolysis and elongation rates at low temperature.
Sujet(s)
Séquence d'acides aminés/génétique , Arthrobacter/génétique , Protéines bactériennes/génétique , Climat froid , Facteur G d'élongation de la chaîne peptidique/génétique , Protéines bactériennes/composition chimique , Micrococcus luteus/génétique , Modèles moléculaires , Données de séquences moléculaires , Mycobacterium tuberculosis/génétique , Facteur G d'élongation de la chaîne peptidique/composition chimique , Structure tertiaire des protéines , Alignement de séquences , Analyse de séquence d'ADN , Similitude de séquences d'acides aminés , Relation structure-activitéRÉSUMÉ
DEAD-box RNA helicases, by unwinding duplex RNA in bacteria and eukaryotes, are involved in essential cellular processes, including translation initiation and ribosome biogenesis, and have recently been implicated in enabling bacteria to survive cold-shock and grow at low temperature. Despite these critical physiological roles, they have not been characterized in archaea. Due to their presumed importance in removing cold-stabilised secondary structures in mRNA, we have characterised a putative DEAD-box RNA helicase gene (deaD) from the Antarctic methanogen, Methanococcoides burtonii. The encoded protein, DeaD is predicted to contain a core element involved in ATP hydrolysis and RNA-binding, and an unusual C-terminal domain that contains seven perfect, trideca-peptide, direct repeats that may be involved in RNA binding. Alignment and phylogenetic analyses were performed on the core regions of the M. burtonii and other DEAD-box RNA helicases. These revealed a loose but consistent clustering of archaeal and bacterial sequences and enabled the generation of a prokaryotic-specific consensus sequence. The consensus highlights the importance of residues other than the eight motifs that are often associated with DEAD-box RNA helicases, as well as de-emphasising the importance of the "A" residue within the "DEAD" motif. Cells growing at 4 degrees C contained abundant levels of deaD mRNA, however no mRNA was detected in cells growing at 23 degrees C (the optimal temperature for growth). The transcription initiation site was mapped downstream from an archaeal box-A element (TATA box), which preceded a long (113 nucleotides) 5'-untranslated region (5'-UTR). Within the 5'-UTR was an 11 bp sequence that closely matches (nine out of 11) cold-box elements that are present in the 5'-UTRs of cold-shock induced genes from bacteria. To determine if the archaeal 5'-UTR performs an analagous function to the bacterial 5'-UTRs, the archaeal deaD 5'-UTR was transcribed in E. coli under the control of the cspA promoter and transcriptional terminator. It has previously been reported that overexpression of the cspA 5'-UTR leads to an extended cold-shock response due to the 5'-UTR titrating cellular levels of a cold-shock repressor protein(s). In our hands, the cold-shock protein profiles resulting from overexpression of Escherichia coli cspA and M. burtonii deaD 5'-UTRs were similar, however they did not differ from those for the overexpression of a control plasmid lacking a 5'-UTR. In association with other recent data from E. coli, our results indicate that the role of the 5'-UTR in gene regulation is presently unclear. Irrespective of the mechanisms, it is striking that highly similar 5'-UTRs with cold-box elements are present in cold induced genes from E. coli, Anabaena and M. burtonii. This is the first study examining low temperature regulation in archaea and provides initial evidence that gene expression from a cold adapted archaeon involves a bacterial-like transcriptional regulatory mechanism. In addition, it provides the foundation for further studies into the function and regulation of DEAD-box RNA helicases in archaea, and in particular, their roles in low temperature adaptation.
Sujet(s)
Basse température , Régulation de l'expression des gènes archéens/génétique , Methanosarcinaceae/enzymologie , Methanosarcinaceae/génétique , RNA helicases/composition chimique , RNA helicases/génétique , Régions 5' non traduites/génétique , Régions 5' non traduites/physiologie , Adaptation physiologique/génétique , Motifs d'acides aminés , Séquence d'acides aminés , Régions antarctiques , Protéines d'archée/biosynthèse , Protéines d'archée/génétique , Protéines bactériennes/génétique , Séquence nucléotidique , Clonage moléculaire , Séquence consensus/génétique , Dosage génique , Gènes d'archée/génétique , Données de séquences moléculaires , Phylogenèse , RNA helicases/métabolisme , ARN messager/analyse , ARN messager/génétique , Éléments de réponse/génétique , Alignement de séquences , Transcription génétique/génétiqueRÉSUMÉ
Despite the presence and abundance of archaea in low-temperature environments, little information is available regarding their physiological and biochemical properties. In order to investigate the adaptation of archaeal proteins to low temperatures, we purified and characterized the elongation factor 2 (EF-2) protein from the Antarctic methanogen Methanococcoides burtonii, which was expressed in Escherichia coli, and compared it to the recombinant EF-2 protein from a phylogenetically related thermophile, Methanosarcina thermophila. Using differential scanning calorimetry to assess protein stability and enzyme assays for the intrinsic GTPase activity, we identified biochemical and biophysical properties that are characteristic of the cold-adapted protein. This includes a higher activity at low temperatures caused by a decrease of the activation energy necessary for GTP hydrolysis and a decreased activation energy for the irreversible denaturation of the protein, which indicates a less thermostable structure. Comparison of the in vitro properties of the proteins with the temperature-dependent characteristics of growth of the organisms indicates that additional cytoplasmic factors are likely to be important for the complete thermal adaptation of the proteins in vivo. This is the first study to address thermal adaptation of proteins from a free-living, cold-adapted archaeon, and our results indicate that the ability of the Antarctic methanogen to adapt to the cold is likely to involve protein structural changes.