Biological Significance of Probiotic Microorganisms from Kefir and Kombucha: A Review.

(1) Background: The human microbiota is essential for maintaining a healthy body. The gut microbiota plays a protective role against pathogenic bacteria. Probiotics are live microorganisms capable of preventing and controlling gastrointestinal and balancing the immune system. They also aid in better nutrients and vitamins absorption. Examples of natural probiotic cultures are kefir and kombucha. (2) Methods: Therefore, the aim of this review was to address the beneficial properties of probiotic kefir and kombucha using a Boxplot analysis to search for scientific data in the online literature up to January 2024: (Latin American and Caribbean Health Sciences (LILACS), PubMed, Medical Literature Analysis (MED-LINE), Science Direct, Google Scholar/Google Academic, Bioline Inter-national and Springer Link). Boxplots showed the summary of a set of data "Index Terms-Keywords" on kefir and kombucha in three languages (English, Portuguese and Spanish). (3) Results: Google Scholar was the database with the highest number of articles found, when the search for the keywords used in the study (containing ~4 × 106-~4 million articles available). This was Followed by the Science Direct database, containing ~3 × 106-~3 million articles available, and the BVS databases-Biblioteca Virtual de Saúde (Virtual Health Library) e Lilacs, both containing a value of ~2 × 106-~2 million articles available. The databases containing the smallest number of articles found were Nutrients and Medline, both containing a value of ≤0.1 × 106-≤100 thousand articles. (4) Conclusions: Scientific studies indicate that kefir and kombucha certainly contain various functional properties, such as antimicrobial, antitumor, anticarcinogenic and immunomodulatory activity, in addition to having a microbiological composition of probiotic bacteria and yeasts. Kefir and kombucha represent key opportunities in the food and clinic/medical fields.

An integrative review of Açaí (Euterpe oleracea and Euterpe precatoria): Traditional uses, phytochemical composition, market trends, and emerging applications.

The increasing trade and popularity of açaí prompt this review. Therefore, it is imperative to provide an overview of the fruit's characteristics and the available data on its marketing, research, and products derived from its pulp and seeds to comprehend the current state of the açaí industry. Concerning food applications, it was observed that there is still room for developing processes that effectively preserve the bioactive compounds of the fruit while also being economically feasible, which presents an opportunity for future research. A notable research trend has been focused on utilizing the fruit's seeds, a byproduct of açaí processing, which is still considered a significant technological challenge. Furthermore, the studies compiled in this review attest to the industry's considerable progress and ongoing efforts to demonstrate the various properties of açaí, driving the sector's exponential growth in Brazil and worldwide.

Biochar production from microalgae: a new sustainable approach to wastewater treatment based on a circular economy.

The generation of wastewater due to human activities are the main responsible for environmental problems. These problems are caused by the large amount of organic and inorganic pollutants related to the presence of pesticides, metals, pathogens, drugs and dyes. The photosynthetic treatment of effluents emerges as a sustainable and low-cost alternative for developing wastewater treatment systems based on a circular economy. Chemical compounds present in wastewater can be recovered and reused as a source of nutrients in microalgae cultivation to produce value-added bioproducts. The microalgal biomass produced in the cultivation with effluents has the potential to produce biochar. Biochar is carbon-rich charcoal that can be obtained by converting microalgae biomass through thermal decomposition of organic raw material under limited oxygen supply conditions. Pyrolysis, torrefaction, and hydrothermal carbonization are processes used for biochar synthesis. The application of microalgal biochar as an adsorbent material to remove several compounds present in effluents is an effective and fast treatment. This effectiveness is usually related to the unique physicochemical characteristics of the biochar, such as the presence of functional groups, ion exchange capacity, thermal stability, and high surface area, volume, and pore area. In addition, biochar can be reused in the adsorption process or applied in agriculture for soil correction. In this context, this review article describes the production, characterization, and use of microalgae biochar through a sustainable approach to wastewater treatment, emphasizing its potential in the circular economy. In addition, the article approaches the potential of microalgal biochar as an adsorbent material and its reuse after the adsorption of contaminants, as well as highlights the challenges and future perspectives on this topic.

Use of exogenous substrate in Chlorella cultivation: Strategy for biomass and polyhydroxybutyrate production.

The aim of this study was to investigate the influence of exogenous carbon supplementation and nitrogen source reduction on Chlorella fusca LEB 111 growth, biomass composition, and polyhydroxybutyrate accumulation. First, assays were performed with 50 % and 25 % reduced nitrogen source concentrations (NaNO3). In the second stage, the influence of culture supplementation with 10, 20, and 30 mg L-1 D-xylose, associated with 50 and 25 % reductions in NaNO3, was evaluated. The experiments conducted with a 25 % reduction in NaNO3 and supplementation with 10 mg L-1 D-xylose resulted in a positive effect on the biomass productivity of C. fusca LEB 111, with production as high as 354.4 mg L-1 d-1. The maximum concentration of PHB extracted from C. fusca LEB 111 was 3.7 % (w w-1) and was obtained when the microalgae were cultivated with a 25 % of reduction in NaNO3 and supplementation of D-xylose at 20 mg L-1. Therefore, this study brings new perspectives regarding reducing the use of nutritional sources and using exogenous carbon sources in using microalgae to produce molecules of high biotechnological potential.

Cost Reduction in the Production of Spirulina Biomass and Biomolecules from Indole-3-Acetic Acid Supplementation in Different Growth Phases.

Despite the great potential for the industrial application of microalgae, production costs are still too high to make them a competitive raw material for commodities. Therefore, studying more efficient cultivation strategies in biomass production and economic viability is necessary. In this sense, this work aimed to reduce the production costs of biomass and biomolecules using phytohormone indole-3-acetic acid in different phases of Spirulina sp. LEB 18 cultivation. The experiments were conducted on bench scale indoor for 30 days. In each couple of experiments, the phytohormone was added on different days. The supplementation of indole-3-acetic acid on half of the growth deceleration phase of the microalga showed a cost reduction of 27%, 34%, and 75% for biomass, proteins, and carbohydrates, respectively. In addition, the strategy increased the final biomass concentration and carbohydrate content at 31.2 and 33.8%, respectively, compared to the condition without phytohormone. This study is the starting point for implementing phytohormone supplementation in industrial microalgal cultures.

Phenolic compounds and antioxidant capacity of Pediastrum boryanum (Chlorococcales) biomass.

Bioactive compounds, synthesized by photosynthetic microorganisms, have drawn the attention of the pharmaceutical field. This study aimed at evaluating synthesis and in vitro antioxidant capacity of phenolic compounds produced by a microalgae species P. boryanum, which was grown in six different culture media (standard BG11, modified BG11/MBG11, standard WC, modified WC, WC*2 and basal). The highest concentrations of biomass (1.75 ± 0.01 g.L-1) and phenolic content (3.18 ± 0.00 mg.g-1) were obtained when P. boryanum was grown in MBG11 and phenolic acids were identified: gallic, protocatechuic, chlorogenic, hydroxybenzoic and vanillic ones. All extracts exhibited scavenger activity in the ABTS assay and inhibited peroxidase. However, phenolic compounds from P. boryanum grown in BG11 and MBG11 had the most potent scavenger activity in the DPPH assay. In sum, P. boryanum can be a new source of free phenolic compounds with potential antioxidant activity when grown in MBG11, since it yields high amounts of biomass and phenolic compounds.

Magnetic fields exhibit a positive impact on lipid and biomass yield during phototrophic cultivation of Spirulina sp.

This study aimed to investigate the effects of magnetic field (MF) application (1, 12 and 24 h day -1) to Spirulina sp. LEB 18 in different photosynthesis cycles (dark and/or light) during short (15 days) and long periods (50 days) of cultivation. MF application was performed via two sources: ferrite magnets and solenoids. At the end of cultivation, the biomass was characterized in terms of lipids, proteins, and carbohydrates. In the 15 day cultures, the highest maximum biomass concentrations (2.06 g L-1 and 1.83 g L-1) were observed when 30 mT was applied for 24 h day -1 or 12 h day -1 (on the light cycle), respectively. MF application throughout cultivation (24 h day -1) for more than 30 days is not recommended. In all conditions, there was an increase in the lipid concentration (from 14 to 45%). The protein profile suggested important changes in photosystems I and II due to MF application. Cell morphology was not altered by MF application. In conclusion, the effects on the metabolism of Spirulina sp. are directly related to the photosynthesis cycle and time period in which the MF was applied.

Magnetic field as promoter of growth in outdoor and indoor assays of Chlorella fusca.

This study aimed to evaluate the influence of a magnetic field (MF) intensity of 25 mT on Chlorella fusca cultivation in outdoor and indoor conditions, and evaluate the changes in the macromolecules, pigment content and protein profile. C. fusca was cultivated for 15 d in raceway photobioreactor. MF was applied for 24 h d-1 and 1 h d-1. In outdoor cultivation, MF applied for 24 h d-1 increased 23% in the biomass concentration, while indoor assays resulted in an increase in both modes, with biomass production increasing between 70 and 85%. Biomass composition was altered when MF was applied for 1 h d-1 in indoor assays; the highest protein content was achieved (32.7%). Nitrate consumption was higher in outdoor assays, while MF application did not alter the protein profile. The results showed that combining the outdoor conditions with MF is advantageous, as higher biomass concentration can be achieved with lower energy expenditure.

Spirulina sp. LEB 18-extracted phycocyanin: Effects on liposomes' physicochemical parameters and correlation with antiradical/antioxidant properties.

This study describes the physicochemical properties of soybean asolectin (ASO) liposomes loaded with phycocyanin (Phy) extracted from Spirulina sp. LEB 18. The effects of Phy in the liposomes' properties were investigated by Fourier transform infrared spectroscopy (FTIR), 1H and 31P nuclear magnetic resonance (NMR), zeta (ζ)-potential, dynamic light scattering (DLS) and ultraviolet-visible (UV-vis) techniques. Phy restricted the motion of ASO polar and interface groups and disrupted the package arrangement of the lipid hydrophobic regions, as a likely effect of dipolar and π interactions related to its amino acid residues and pyrrole portions. These interactions were correlated to antiradical/antioxidant Phy responses obtained by 2,2-diphenyl-1-picrylhidrazil (DPPH) assay, thiobarbituric acid reactive substances (TBARS) and ferric reducing antioxidant power (FRAP) methods, and discussed to bring new chemical perspectives about Phy-loaded liposomes-related nutraceutical applications in inflammatory and viral infection processes.

Renewal of nanofibers in Chlorella fusca microalgae cultivation to increase CO2 fixation.

This study explored strategies to increase the CO2 fixation ability of microalgae by renewing polymeric nanofibers in cultures of Chlorella fusca LEB 111. Nanofibers composed of 10% (w v-1) polyacrylonitrile (PAN)/dimethylformamide (DMF) containing 4% (w v-1) iron oxide nanoparticles (NPsFe2O3) were added to photobioreactors. The nanomaterial was renewed in the test cultures as follows: renewal only on day 7; renewal only on day 15; or renewal on both days 7 and 15 (i.e., double renewal). The highest biomass concentration (2.53 g L-1) and CO2 biofixation rate (141.5 mg L-1 d-1) were obtained by cultivating with double renewal, resulting in values 21.6% and 23% higher, respectively, than those obtained by cultivation without renewal. The application of nanofiber renewal in the cultivation of C. fusca LEB 111 shows the potential to increase CO2 biofixation, which may contribute to reducing the atmospheric concentrations of this main greenhouse gas intensifier.

Increased lipid synthesis in the culture of Chlorella homosphaera with magnetic fields application.

This study aimed to investigate the influence of different intensities (15, 30 and 60 mT) and exposure times (1 h d-1, 24 h d-1) of magnetic fields (MF) on the stimulation of lipid synthesis by the microalga Chlorella homosphaera. The growth and biochemical characterization of protein, carbohydrate and lipid content were determined. Biomass concentration increased by 20.6% (30 mT, 1 h d-1) and 12.4% (60 mT, 1 h d-1) in the presence of MF. However, biomass decreased by 33.0% (15 mT, 1 h d-1) in relation to control cultivation (CC). The stress caused by the MF application stimulated lipid synthesis and biomass production. In all evaluated conditions, MF application showed a positive effect on lipid production; the application of 60 mT or 30 mT for 1 h d-1 increased lipid productivity by 108.4% and 135.1%, respectively. MF application with ferrite magnets was thus efficient to stimulate lipid synthesis.

Application of Static Magnetic Fields on the Mixotrophic Culture of Chlorella minutissima for Carbohydrate Production.

Magnetic field (MF) can interact with the metabolism of microalgae and has an effect (positive or negative) on the synthesis of molecules. In addition to MF, the use of pentose as a carbon source for cultivating microalgae is an alternative to increase carbohydrate yield. This study aimed at evaluating the MF application on the mixotrophic culture of Chlorella minutissima in order to produce carbohydrates. MF of 30 mT was generated by ferrite magnets and applied diurnally for 12 days. The addition of 5% pentose, MF application of 30 mT, and nitrogen concentration reduced (1.25 mM of KNO3) was the best conditions to obtain higher carbohydrate concentrations. MF application of 30 mT increased biomass and carbohydrate contents in 30% and 163.1%, respectively, when compared with the assay without MF application. The carbohydrate produced can be used for bioethanol production.

Development of a colorimetric pH indicator using nanofibers containing Spirulina sp. LEB 18.

A colorimetric pH indicator was developed using nanofibers of poly(lactic acid) (PLA) and polyethylene oxide (PEO) combined with biomass of the microalga Spirulina sp. LEB 18. This study evaluates the potential use of microalgal biomass encapsulated in polymer nanofibers to develop a colorimetric pH indicator. Nanofibers containing the biomass were exposed to solutions with different pH values (pH 1-10), and color variations were measured using a colorimeter. The wettability analysis of the nanofibers showed hydrophilicity (zero angle with water), which allows ions to interact with the biomass, indicating a fast color response as a function of pH. When subjected to pH variations, indicators containing 1, 2 or 3% (w v-1) of biomass provided ΔΕ values >12, indicating an absolute difference in color. Therefore, this innovative material has the potential to be applied as a intelligent indicator to verify food quality through a visual signal of the product condition.

Polyhydroxybutyrate production and increased macromolecule content in Chlamydomonas reinhardtii cultivated with xylose and reduced nitrogen levels.

The aim of the current study was to evaluate the production of macromolecules such as polyhydroxybutyrate under pentose supplementation and reduced nitrogen levels in Chlamydomonas reinhardtii. Two batches of experiments were carried out: (1) reduction in the nitrogen (NH4Cl) concentration to 6 and 4 g L-1 and (2) supplementation of 10, 20 and 30 mg L-1 D-xylose together with a reduction in the NH4Cl concentration (6 and 4 g L-1). The addition of 20 mg L-1 D-xylose together with 6 g L-1 NH4Cl resulted in polyhydroxybutyrate production (206.0 mg L-1). The reduction of 8 to 6 g L-1 NH4Cl did not trigger a reduction in the production of either proteins (68.3% w w-1) or carbohydrates (23.3% w w-1) in the cells. The current study demonstrated that nutritional modifications, which until now have been unexplored in C. reinhardtii, triggered the production of macromolecules (polymers, carbohydrates and proteins) with high biotechnological potential.

Spirulina sp. LEB 18 cultivation in a raceway-type bioreactor using wastewater from desalination process: Production of carbohydrate-rich biomass.

This study aimed to evaluate the biomass production of Spirulina sp. LEB 18 cultivated in wastewater from the desalination process. The outdoor cultivations (210 L) were performed using as culture medium 100% wastewater supplemented with 25% of Zarrouk constituents (Tcs). In parallel, it was performed a control assay using 100% Zarrouk constituents. The biomass production in Tcs assay (1.14 g L-1) was only 9% lower than the control assay (1.25 g L-1). The Tcs assay showed a higher content of carbohydrates (52.29%), lipids (12.79%) and ash (2.69%) compared to the control assay (47.91; 7.59 and 1.29%, respectively). The biomass from the control and Tcs assays had mostly monounsaturated fatty acids C15:1 and C18:2n6t. The Spirulina sp. LEB 18 could use efficiently the nutrients from the wastewater, showing high removal efficiency of NO3- (96.99%), PO4 (83.11%) and Z (96.43%). At the same time, high added value biomolecules were produced for different purposes.

Role of light emitting diode (LED) wavelengths on increase of protein productivity and free amino acid profile of Spirulina sp. cultures.

LEDs have specific wavelengths that can positively influence the production of microalga biomass and biomolecules of interest. Filling the gaps in the literature, this study evaluated the effect of different LED wavelengths and photoperiods on protein productivities and free amino acid (FAA) profile of Spirulina sp. LEB 18 cultures. The best protein productivity results were obtained in red and green LED cultures using integral and partial photoperiods, respectively. In these experiments, protein productivities increased 2 and 1.6 times, respectively, compared to the control culture using fluorescent light. Green LEDs in partial photoperiod provided also the highest concentrations of essential and non-essential FAA, about 1.8 and 2.3 times higher, respectively, than control cultures. LEDs showed to be a promising sustainable light source for increasing protein productivity and FAA concentration in Spirulina sp. LEB 18 cultures.

Magnetic fields: biomass potential of Spirulina sp. for food supplement.

This study evaluated the influence of the magnetic field on the chemical composition of Spirulina sp. LEB 18 and its digestibility and protein solubility. The highest protein digestibility of biomass was obtained at 30 °C and with 2.5 g L-1 NaNO3 (78.4%) in the medium, and the highest solubility was found in the cultivated biomass exposed to 60 mT, 30 °C and 2.5 g L-1 NaNO3 (89%, pH 6). MF application did not modify the protein concentration of biomass, but reduced the carbohydrate concentration by 69.1%, showing that the biomass obtained in the culture submitted to MF may be used as an ingredient in the development of protein supplements.

Progress in the physicochemical treatment of microalgae biomass for value-added product recovery.

Interest in microalgae-derived products is growing, mostly due to their unique characteristics and range of industrial applications. To obtain different products, one must employ specific pretreatments that retain the properties of the biologically active compounds extracted from microalgae biomass; thus, new extraction techniques require frequent upgrades. Due to increased interest in economically viable and ecologically friendly processes, new extraction methods that can be incorporated into microalgae biorefinery systems have become the main focus of research. Therefore, this review aims to address the potential applications, future prospects, and economic scenario of the new physicochemical treatments used in the extraction of bioactive microalgae compounds.

Microalgae starch: A promising raw material for the bioethanol production.

Ethanol is currently the most successful biofuel and can be produced from microalgal biomass (third-generation). Ethanol from microalgal biomass has advantages because it does not use arable land and reduces environmental impacts through the sequestration of CO2 from the atmosphere. In this way, micro and macroalgal starch, which is structurally similar to that from higher plants can be considered a promise raw material for the production of bioethanol. Thus, strategies can be used to intensify the carbohydrate concentration in the microalgal biomass enabling the production of third-generation bioethanol. The microalgae biomass can be destined to biorefineries so that the residual biomass generated from the extraction processes is used for the production of high value-added products. Therefore, the process will have an impact on reducing the production costs and the generation of waste. In this context, this review aims to bring concepts and perspectives on the production of third-generation bioethanol, demonstrating the microalgal biomass potential as a carbon source to produce bioethanol and supply part of the world energy demand. The main factors that influence the microalgal cultivation and fermentation process, as well as the processes of transformation of biomass into the easily fermentable substrate are also discussed.

Brackish Groundwater from Brazilian Backlands in Spirulina Cultures: Potential of Carbohydrate and Polyunsaturated Fatty Acid Production.

The composition of brackish groundwater from Brazilian backlands contains important elements necessary for metabolism in microalgae. This study evaluated the use of 100% brackish groundwater with different amounts of Zarrouk nutrients for Spirulina sp. LEB 18 cultivation. The growth parameters and biomass composition, including the concentrations of proteins, carbohydrates, ash, lipids, and fatty acids, were evaluated. The best growth parameter results were obtained in the assay using 100% brackish groundwater and only 25% of Zarrouk nutrients, which were equal to those obtained for the control culture. The concentrations of carbohydrates and polyunsaturated fatty acids were increased by as much as 4- and 3.3-fold, respectively, when brackish groundwater was used in the cultures. The lipid profile demonstrated that the biomass had the potential for use in biodiesel production. The use of brackish groundwater is a sustainable, economical way to obtain high-quality biomass for different applications during Spirulina sp. LEB 18 cultivation.