High Seroprevalence of Lawsonia intracellularis in Thoroughbred Farms in Southern Brazil.

The aim of the present study was to carry out a serological survey to identify the seroprevalence of Lawsonia intracellularis in six Thoroughbred farms in the Southern region of the state of Rio Grande do Sul, Brazil. During 2019 and 2020, blood samples from 686 Thoroughbred horses were obtained from six different breeding farms. Horses were divided into groups according to age: (1) broodmares (>5 years), (2) two-year-old foals, (3) yearlings, and (4) 0-6 months-old foals. Blood samples were collected by venipuncture of the external jugular vein. The detection of antibodies (IgG) against L. intracellularis was performed by Immunoperoxidase Monolayer Assay. The detection of specific antibodies (IgG) against L. intracellularis in the evaluated population was 51%. The highest detection (86.8%) of IgG was in the broodmares category, while the lowest (5.2%) was in foals of 0-6 months of age. Regarding the farms, the Farm 1 had the highest (67.4%) prevalence of seropositivity against L. intracellularis, while Farm 4 had the lowest (30.6%). There was no record of clinical manifestation of Equine Proliferative Enteropathy in the sampled animals. The results of this study show the high seroprevalence of L. intracellularis in Thoroughbred farms in the Southern of Rio Grande do Sul, suggesting a large and continuous exposure to the agent.

Humoral immunity is not altered in overweight pregnant Crioulo mares / Imunidade humoral não é alterada em éguas Crioulas gestantes com sobrepeso

Both pregnancy and obesity can influence significant changes in the immune system. On this basis, the present study proposes to evaluate the humoral immune response of overweight pregnant mares in response to a commercial vaccine. Thirty pregnant Crioulo mares were separated according to body condition score (BCS) into overweight (BCS≥7/9) or lean-control (BCS= 5-6/9). In each group, the animals were subdivided into vaccinated and controls. The mares were vaccinated against EHV-1 in two doses spaced 21 days apart and had their blood collected monthly, for five months, for antibody evaluation. Both vaccinated groups had an increase in specific neutralizing antibodies after the vaccine. However, after the second dose, there was no increase in antibodies in any of the groups. Vaccinated overweight and lean-control mares did not differ at any time point. Therefore, this study demonstrated that obesity does not influence the humoral immune response in pregnant Crioulo mares.(AU)

Tanto a gestação quanto a obesidade podem influenciar o desenvolvimento de alterações significativas no sistema imune, portanto, o presente estudo teve como objetivo avaliar a resposta imune humoral de éguas gestantes com sobrepeso em resposta a uma vacina comercial. Trinta éguas Crioulas gestantes foram separadas de acordo com o escore de condição corporal (ECC) em éguas com sobrepeso (ECC≥7/9) e éguas controles (ECC=5-6/9) e, ainda, em cada grupo, os animais também foram separados em vacinados e controles. As éguas foram vacinadas contra o EHV-1 em duas doses com intervalo de 21 dias, sendo realizadas coletas de sangue mensalmente durante cinco meses para avaliação de anticorpos neutralizantes. Ambos os grupos vacinados tiveram aumento de anticorpos neutralizantes específicos após a vacina, porém, após a segunda dose, não foi observado aumento de anticorpos em nenhum dos grupos. Nenhuma diferença foi observada entre éguas vacinadas com sobrepeso e as éguas controles em nenhum momento. Assim, este estudo demonstrou que a obesidade não é um fator que influencia a resposta imune humoral de éguas Crioulas gestantes.(AU)

Body and testicular biometry of Crioulo stallions / Biometria corporal e testicular de garanhões da raça Crioula

Brazilian Association of Crioulo Horse Breeders (ABCCC) establishes minimal and maximal body biometrics evaluation; although, nothing is mentioned about testicular measurements. Body and testicular growth are associated and related to age. We described data regarding body and testicular biometry of Crioulo stallions, with a comparative study between young and adult categories. Evaluation was performed in 56 stallions, Crioulo breed, split in: youngsters (3 and 4 years-old, n=16) and adults (above 5 years-old, n=40). Body biometry included weight, height, cannon bone and chest circumference, body score condition and neck fat accumulation. Testicular biometry included height, length, width, volume, combined volume and daily sperm output (DSO). Statistical analyses included descriptive statistic, Pearson correlation, comparison between means by Kruskal Wallis, being P < 0.05 considered significant. There was no significant difference between the parameters of body and testicular biometrics between categories young and adult. In relation to height, the average was very close to the lower limit established, while chest and cannon bone circumference were above the minimum recommended by ABCCC. Cannon bone circumference presented a positive correlation with height. Most of stallions presented excessive body fat, with a body score condition above 8 (scale 1-9). Neck fat accumulation presented a positive correlation with body score condition. Testicular height, length, width and volume presented a positive correlation between the ipsi and contralateral testicle, total testicular volume and DSO. In conclusion, no difference in the body and testicular biometric evaluation was observed between young and adult Crioulo stallions. Testicular and body growth are associated and also related to age, so our finding suggested that after 3-4 years-old most of Crioulo stallions have already reached maximum growth.

A Associação Brasileira de Criadores de Cavalos Crioulos (ABCCC) define parâmetros mínimos e máximos para algumas aferições biométricas no corpo do animal, embora nada seja mencionado sobre medidas testiculares. O crescimento corporal e testicular estão associados e relacionados a idade do animal. Objetivamos descrever parâmetros relacionados a biometria corporal e testicular de garanhões Crioulos, comparando o grupo de jovens e adultos. Foi realizada avaliação em 56 garanhões Crioulos, divididos em: jovens (três e quatro anos, n=16) e adultos (acima de cinco anos, n=40). A biometria corporal incluiu peso, altura, circunferência de canela, perímetro torácico, escore corporal e acúmulo de gordura no pescoço. A biometria testicular incluiu altura, comprimento, largura, volume de cada testículo, volume combinado dos testículos e estimativa de produção diária de espermatozoides (DSO). Com os dados, foi realizada estatística descritiva, correlação de Pearson, comparação entre médias com Kruskal Wallis, sendo considerado significativo quando P < 0,05. Não foi observada diferença significativa entre as avaliações biométricas e testiculares de garanhões das categorias jovens e adultos. Em relação à altura, a média foi muito próxima ao limite inferior estabelecido, enquanto a circunferência torácica e de canela estavam acima do limite mínimo recomendado pela ABCCC. A circunferência de canela apresentou correlação positiva com altura. A maioria dos garanhões apresentou alta deposição de gordura, com condição corporal acima de 8 (escala 1-9). O acúmulo de gordura no pescoço apresentou correlação positiva com escore corporal. A altura, largura, comprimento e volume testicular apresentaram correlação positiva com testículo ipsi e contralateral, volume testicular total e DSO. Em conclusão, não houve diferença entre dados de biometria corporal e testicular de garanhões Crioulos adultos e jovens. O crescimento corporal e testicular estão associados e relacionados a idade, sendo assim, nossos resultados sugerem que após 3-4 anos de idade, a maioria dos garanhões Crioulos já atingiu seu pico de desenvolvimento.

Outcome of a ceratohyiodectomy in a criollo mare with temporohyoid osteoarthropathy

Background: Temporohyoid osteoarthropathy (THO) is a progressive disease that causes acute onset of peripheral vestibular signs with or without facial paralysis. Ankylosis of temporhyoid joint occurs which predispose to fractures of theinvolved bones and consequently causes the commonly neurological signs observed. Clinical signs vary depending on thestage of the disease and the nerves affected. Surgical treatment is advised to improve survival rates in which the ceratohyoidectomy is currently known as the most advantageous. The aim of the present study is to report a case and outcome ofa ceratohyoidectomy procedure in a Criollo mare presenting THO of the right temporohyoid joint.Case: A 17-year-old Criollo mare was referred to the Equine Clinical Hospital of the Federal University of Pelotas witha 5-day history of facial paralysis on the right side, head tilt and difficulty to chew and swallow. Auricular, palpebraland labial ptosis along with deviation of the lip and nostril to the left were observed. A corneal ulcer was also identifiedin the right eye. Complementary imaging exams (endoscopy of the guttural pouches and radiography of the head) wereperformed and showed thickening of the right stylohyoid bone confirming a diagnosis of THO. Anti-inflammatory andantibiotic therapy were administered and the corneal ulcer was treated with topical antibiotics and autologous serum. Dueto rapid deterioration of clinical signs, the mare was referred to surgery. A ceratohyoidectomty procedure was performedunder general anesthesia. In this procedure, the ceratohyoid bone was disarticulated from the ceratohyoid-basihyoid jointand removed. During the procedure, a branch of the linguofacial vein was accidentally incised causing hemorrhage, thebranch was identified and successfully ligated. Recovery was uneventful. Supportive treatment with anti-inflammatory andantibiotics was continued after surgery and ...(AU)

Changes in cholesterol, triglycerides and body composition in pregnant mares

Background: Mares are very different from other species during pregnancy, and studies on the physiologicalchanges of this period are important. During late pregnancy, the distribution of weight and body fat are oftenused as indicators of adequate nutrition. This is a physiological period that results in an increase in metabolicdemand. There is a tendency for the Criollo breed to have a higher body condition score that becomes moreevident during pregnancy, a period when mares tend to gain more weight. The current study monitored serumcholesterol and triglyceride levels in pregnant mares during late gestation to determine a possible correlationwith the distribution of fat or body weight.Materials, Methods & Results: Four body parameters of 34 Criollo-type mares in late gestation were evaluated: body weight measured with a weight scale, body weight using a commercial weight tape, total body fatand fat thickness and the serum levels of total cholesterol and triglycerides. The fat thickness was measuredin an ultrasound device and the prediction of total body fat was calculated using an equation. According to thedays prior foaling, biometric monitoring and blood collection were carried out in five periods: F-90 (± 90 daysprior to foaling) n = 33; F-60 (± 60 days prior to foaling) n = 33; F-30 (± 30 days prior to foaling) n = 31; F-15(± 15 days prior to foaling) n = 29 and Foaling (at day of foaling) n = 14. Mares were monitored daily andaccompanied foaling was also performed, ensuring collection at the right time. Comparisons of means wereperformed between variables in addition to the Pearson correlation test. Statistical significance was establishedat P < 0.05. There was no difference in relation to the period in body composition measurements (P > 0.05). Astrong positive correlation was observed between the average weights (P < 0.001). The fat thickness showeda correlation between the weights (P < 0.01). There was no correlation with...(AU)

Outcome of a ceratohyiodectomy in a criollo mare with temporohyoid osteoarthropathy

Background: Temporohyoid osteoarthropathy (THO) is a progressive disease that causes acute onset of peripheral vestibular signs with or without facial paralysis. Ankylosis of temporhyoid joint occurs which predispose to fractures of theinvolved bones and consequently causes the commonly neurological signs observed. Clinical signs vary depending on thestage of the disease and the nerves affected. Surgical treatment is advised to improve survival rates in which the ceratohyoidectomy is currently known as the most advantageous. The aim of the present study is to report a case and outcome ofa ceratohyoidectomy procedure in a Criollo mare presenting THO of the right temporohyoid joint.Case: A 17-year-old Criollo mare was referred to the Equine Clinical Hospital of the Federal University of Pelotas witha 5-day history of facial paralysis on the right side, head tilt and difficulty to chew and swallow. Auricular, palpebraland labial ptosis along with deviation of the lip and nostril to the left were observed. A corneal ulcer was also identifiedin the right eye. Complementary imaging exams (endoscopy of the guttural pouches and radiography of the head) wereperformed and showed thickening of the right stylohyoid bone confirming a diagnosis of THO. Anti-inflammatory andantibiotic therapy were administered and the corneal ulcer was treated with topical antibiotics and autologous serum. Dueto rapid deterioration of clinical signs, the mare was referred to surgery. A ceratohyoidectomty procedure was performedunder general anesthesia. In this procedure, the ceratohyoid bone was disarticulated from the ceratohyoid-basihyoid jointand removed. During the procedure, a branch of the linguofacial vein was accidentally incised causing hemorrhage, thebranch was identified and successfully ligated. Recovery was uneventful. Supportive treatment with anti-inflammatory andantibiotics was continued after surgery and ...

Occurrence of Gasterophilus spp. in weanling foals in southern Brazil

The occurrence of gastrointestinal myiasis caused by Gasterophilus spp. larvae (Diptera: Oestridae) in adult horses has been widely characterized, however data on natural infestation in young foals have been lacking. This observation may be related to the absence of conclusive diagnosis in these individuals, most likely due to logistical or financial constraints. Gastric ulceration is a problem and a significant cause of morbidity in foals, particularly during the weaning stage; therefore, gasterophilosis should be included in the differential diagnosis. The primary purpose of this study was to determine the prevalence of gasterophilosis in 4- to 6-month-old weanling foals. Seventy-one healthy weanling foals were enrolled in the study. Physical assessment blood sampling and was completed in all foals before the commencement of the experiment. Gastroscopy examinations were performed under sedation (Detomidine 0.01 - 0.02 mg/kg) with a flexible endoscope inserted through nasogastric via. The lumen of the stomach was examined in order to search for botflies' larvae. Following gastroscopy, foals were classified into 2 groups based on the presence of Gasterophilus spp. larvae in their stomachs: 1) Infected and 2) Not Infected. Infected foals received a single dose of commercial trichlorfon and albendazole equine oral gel and were stalled for 24 h. The passed feces were thoroughly examined, searching for elimination of larvae. A total of 64% of the foals (n = 45/71) harbored Gasterophilus spp. larvae in the stomach. Mild hyperemia in the gastric mucosa was observed in the attachment sites of the parasites. Physical assessment and hematological parameters' data were analyzed with Shapiro-Wilk normality test. Comparison between groups for clinical signs, hematological parameters and Gasterophilus spp. infection rates were evaluated using the Kruskal-Wallis test or Student's t-test. Statistical significance was set at P < 0.05. There were no statistically significant variations in physical and hematological parameters between foals that were affected and those that were not infected. The larvae found in the feces were subjected to morphological examination, which confirmed the diagnosis of Gasterophilus intestinalis. No systemic clinical indications compatible with Gasterophilus spp. infestation were seen, as described in previous studies of horses infected with the parasite. No botfly eggs were observed in the hair of evaluated foals. During gastroscopy, mild hyperemic lesions in the gastric mucosa were observed in the larvae fixation sites. Although no changes in clinical or hematological parameters were noted, the confirmation of parasite presence is a cause for concern due to horses' tolerance for low infestation levels and poor diagnosis. Additionally, the presence of this myiasis in foals may be a significant stressor during the weaning period and should be included in the differential diagnosis of recurring abdominal pain. Furthermore, infected foals might be a reservoir for the parasite and, contribute to the elimination and spread of the larvae in the environment. Thus, inclusion of young horses in deworming protocols targeted to botfly larvae is needed. This is the first report of Gasterophilus intestinalis myiasis in foals in Brazil. Further research is necessary to fully understand the epidemiology and prevalence of this condition in young horses in Brazil, based on the findings of this study.(AU)

Complete avulsion of the hoof capsule and subsequent testicular degeneration in a criollo stallion

Background: Complete avulsion of the hoof in horses, also known as exungulation, is not a commonly reported injury and usually leads to euthanasia due to the great amount of tissue loss, intense pain, secondary complications, expensive and lengthy treatment. It can involve deep structures and cause different complications leading to chronic lameness. In stallions affected by such injury, the reproductive tract and performance may also be affected. The aim of this study was to report a case of complete avulsion of the right front hoof in a Criollo stallion and subsequent bilateral testicular degeneration. Case: A 10-year-old Criollo stallion was referred to the Veterinary Clinical Hospital of the Federal University of Pelotas (HCV- UFPel) with a complete avulsion of the left front hoof. At admission, the stallion had clinical parameters compatible with intense pain and blood loss. Evaluation of the wound demonstrated that the distal end of the third phalanx (P3) was exposed but no fracture was detected on radiological evaluation. No other structure was apparently affected. Initially, anti-inflammatory (phenylbutazone) and opioid (morphine) was given for pain control and supportive fluid therapy was started to restore hydration. Antibiotic (Sulfamethoxazole with trimethoprim) was administered for 10 days. Continued therapy with phenylbutazone, pentoxifylline, omeprazole and supplementation with methionine, biotin and zinc was also given. Local treatment was carried out by cleaning the wound, applying an antimicrobial ointment and dressing it with a bandage. Wound management was adapted according to the evolution and healing process. The stallion was kept in stall rest during its hospitalization time. In the second month after the injury, accumulation of liquid in the scrotum was observed. Clinical and ultrasound evaluation lead to a presumptive diagnosis of testicular degeneration. The stallion was discharged after three months when the wound was almost healed and the hoof had started to grow. Six month later, a follow up by the referring vet showed that the hoof was almost completely grown and the x-ray assessment demonstrated a dorsal rotation and resorption of the distal end of the third phalanx. Discussion: The stallion of this report had a complete avulsion of the hoof capsule caused by trauma. Conservative treatment was established including wound cleaning and dressing to avoid contamination, control of pain and inflammation, antimicrobial care and supplementation to support hoof growth. Time period for wound healing and hoof growth was in agreement with other cases described previously. Bone sequestrum of the distal end of the third phalanx, and detachment of a fragment were observed in this case, followed by bone resorption. The stallion was closely monitored to prevent laminitis in the contralateral limb and no alterations were detected during the treatment period. Testicular degeneration was observed, probably caused as a consequence of hoof avulsion and due to a long period of stall rest. Degenerative alterations in testicles interfere with thermoregulation and spermatogenesis, affecting semen quality and reproductive performance. Rotation of the third phalanx was also observed six months later caused by the hoof loss. In conclusion, the patient of this report had a complete regrowth of the hoof capsule although a long intensive treatment was necessary to achieve this result. As a consequence, testicles degeneration may happen impairing its function as a stallion.(AU)

Changes in cholesterol, triglycerides and body composition in pregnant mares

Background: Mares are very different from other species during pregnancy, and studies on the physiologicalchanges of this period are important. During late pregnancy, the distribution of weight and body fat are oftenused as indicators of adequate nutrition. This is a physiological period that results in an increase in metabolicdemand. There is a tendency for the Criollo breed to have a higher body condition score that becomes moreevident during pregnancy, a period when mares tend to gain more weight. The current study monitored serumcholesterol and triglyceride levels in pregnant mares during late gestation to determine a possible correlationwith the distribution of fat or body weight.Materials, Methods & Results: Four body parameters of 34 Criollo-type mares in late gestation were evaluated: body weight measured with a weight scale, body weight using a commercial weight tape, total body fatand fat thickness and the serum levels of total cholesterol and triglycerides. The fat thickness was measuredin an ultrasound device and the prediction of total body fat was calculated using an equation. According to thedays prior foaling, biometric monitoring and blood collection were carried out in five periods: F-90 (± 90 daysprior to foaling) n = 33; F-60 (± 60 days prior to foaling) n = 33; F-30 (± 30 days prior to foaling) n = 31; F-15(± 15 days prior to foaling) n = 29 and Foaling (at day of foaling) n = 14. Mares were monitored daily andaccompanied foaling was also performed, ensuring collection at the right time. Comparisons of means wereperformed between variables in addition to the Pearson correlation test. Statistical significance was establishedat P 0.05). Astrong positive correlation was observed between the average weights (P < 0.001). The fat thickness showeda correlation between the weights (P < 0.01). There was no correlation with...

Monitoramento do parto em éguas / Monitoring the parturition in mares

O objetivo do presente é explorar aspectos da fisiologia do final da gestação em éguas, com foco na maturação do feto e monitoramento do parto nessa espécie. Inúmeras são as particularidades na endocrinologia da gestação, que culminam em uma ampla variação no tempo de gestação na égua e uma tardia maturação orgânica no potro, que se completa somente após o nascimento. A primeira fase do parto é considerada prodrômica, marcada pela inquietação e desconforto na égua, e movimentação do potro para apresentação longitudinal anterior, posição dorso-sacra e atitude estendida. A segunda fase do parto é o momento crítico e rápido que inicia com a ruptura do corioalantóide e não deve ultrapassar 30 minutos, desde a ruptura da placenta até a expulsão completa do potro neonato. A terceira fase é caracterizada pela expulsão da placenta, a qual deve ser avaliada pelo obstetra. O monitoramento do potro neonato é marcado pela observação dos reflexos posturais e comportamentais, além do exame físico e exames complementares. O momento do parto é decisivo na vida do potro neonato, sendo necessária a compreensão que o monitoramento dessa etapa é uma estratégia de ação que só alcança sucesso quando realizado de forma continuada ao acompanhamento obstétrico sistemático.

The purpose of this article is to examine the physiological characteristics of the final third of pregnancy in mares, with a particular emphasis on fetal maturation and parturition monitoring. There are numerous peculiarities in the endocrinology of equine pregnancy, culminating in a wide variation in the length of gestation in the mare and a delayed systemic maturation in the foal, which is completed only after birth. The first stage of labor, also known as prodromal stage, is characterized by restlessness and discomfort of the mare, as well as the foal's transition to anterior longitudinal presentation, dorso-sacral position, and extended attitude. The second stage labor is a critical and rapid period that begins with the chorioallantois rupture and should last no more than 30 minutes, from the rupture of the placenta to the complete expulsion of the neonatal foal. The third stage is characterized by the elimination of the placenta, which must be evaluated by the veterinarian. The assessment of the neonatal foal is performed by the observation of righting reflexes, in addition to physical examination and complementary exams. The parturition is a decisive moment in the life of the foal, and it is necessary to understand that monitoring this stage is an action strategy that only achieves success when carried out continuously with systematic obstetric follow-up.

Síndrome do mau ajustamento neonatal em potros: foco em neuroesteróides / Foal mal adjustment syndrome: focus on neurosteroids

O processo de transição do feto para a vida extra-uterina é considerado um período crítico que requer complexas adaptações fisiológicas do potro neonato. Eventos estressores de origem hipóxicoisquêmicas no periparto podem desencadear um quadro de encefalopatia neonatal equina, também conhecida como síndrome do mau ajustamento neonatal. O diagnóstico é feito baseado na avaliação clínica e na anamnese e avaliação do histórico da gestação. Casos leves a moderados tem prognóstico favorável. É imprescindível o entendimento da endocrinologia da gestação, do papel dos neuroesteróides no desenvolvimento do sistema nervoso fetal para que o estabelecimento precoce da terapia adequada seja realizado de maneira bem sucedida. Assim, o objetivo do presente é abordar os principais aspectos clínicos e fisiopatológicos da Síndrome do Mau Ajustamento Neonatal em neonatos equinos, com foco especial no papel dos neuroesteróides durante a maturação cerebral do feto no terço final da gestação e na transição para a vida extra-uterina.

The transition from fetus to extrauterine life is considered a critical period that requires complex physiological adaptations on the part of the newborn foal. Peripartum hypoxic-ischemic stressors can result in equine neonatal encephalopathy, also known to as neonatal maladjustment syndrome. The diagnosis is made based on clinical examination, anamnesis, and a review of the mare’s pregnancy history. Cases that are mild to moderate in severity have a favorable prognosis. It is critical to understand the endocrinology of pregnancy and the role of neurosteroids in the development of the fetal nervous system in order to successfully initiate appropriate therapy early. Thus, the purpose of this article is to discuss the major clinical and pathophysiological aspects of neonatal maladjustment syndrome in equine neonates, with a particular emphasis on the role of neurosteroids during fetal brain maturation in the final third of pregnancy and during the transition to extrauterine life.

Small intestine strangulating obstruction by mesenteric pedunculated lipoma in a criollo mare

Background: The abdominal lipomas are benign tumors of hyperplastic growth that originate from mesenteric adiposetissue, most commonly in the small intestine. Pedunculated lipoma strangulation occurs when the pedicle wraps aroundan intestinal loop and its mesentery, obstructing the intestinal lumen and blood supply of the affected segment. The aim ofthe present study is to report a case of a Criollo mare presenting a strangulating obstruction of the jejunum and ileum bya pedunculated lipoma, focusing the discussion in the causes and epidemiology of this alteration.Case: A 24-year-old Criollo mare with a body condition score 8 out of 9 was referred to the Veterinary Clinical Hospitalof the Federal University of Pelotas (HCV-UFPel) presenting acute abdominal pain. At the hospital, the mare was presenting mild signs of abdominal discomfort. At the initial clinical exam, the mare had an increased heart and respiratory rates,normal body temperature, hyperemic mucous membranes, capillary refil time of 3 s and absent gut sounds in all fourquadrants during auscultation. Mild dehydration was present, increased fibrinogen and serum lactate. Rectal palpationrevealed distention of the small intestine by gas, during abdominal ultrassonography the small intestinal wall thicknesswas increased Peritoneal lactate was 11 mmol/L and protein was 6 mg/dL. Due to the clinical findings during the exam, anexploratory celiotomy was performed. Necrosis of the final third of jejunum and the entire extension of ileum was found.Examining the compromised intestinal portion, it was observed a linear structure strangulating two segments of the smallintestine. Taking into account the extensive area of irreversible necrosis and the advanced age of the patient, euthanasiawas performed. The mare was then referred for necropsy and findings confirmed...(AU)

Gestação gemelar em éguas (parte II): diagnóstico e intervenção / Twin pregnancies in mares, part II: diagnosis and management / Embarazos geme/ares en yeguas, parte II: diagnóstico y manejo

Gestações gemelares em equinos resultam em perdas embrionárias, aborto no terço final ou nascimento de potros debilitados. O objetivo desta revisão é discutir os métodos de diagnóstico e intervenção da gestação gemelar. O diagnóstico deve ser realizado na fase de migração embrionária, entre 12 e 15 dias. A ruptura de uma das vesículas é o método mais eficaz promovendo perdas similares as de uma gestação simples. Caso não seja possível intervir nesta fase, pode-se realizar o acompanhamento até cerca de 30 dias para observar a redução natural. Não ocorrendo a redução, existem técnicas de maior risco que podem ser realizadas, dentre elas a aspiração transvaginal, manipulação craniana do feto, deslocamento craniocervical ou injeção de intracardíaca-pulmonar.A eficiência da manipulação está relacionada a capacidade técnica do profissional e método utilizado, sempre buscando preservar a integridade da égua gestante.

Twin pregnancies in horses result in embryonic losses, abortion in the final third or birth of debilitated foals. The aim of this review is to discuss the diagnosis and intervention methods of twin pregnancy. The diagnosis should be made in the embryonic migration phase, between 12and 15 days. Rupture of one of the vesicles is the most effective method promoting losses similar to those of a single pregnancy. If it is not possible to manipulate at this stage, observations can be done for up to 30 days to observe the natural reduction. If there is no reduction, there are higher risk techniques that can be performed, including transvaginal aspiration, cranial manipulation of the fetus, craniocervical dislocation or intracardiac-pulmonary injection. The efficiency of manipulationis related to the professional's technical capacity and method, always seeking to preserve the integrity of the pregnant mare.

Los embarazos gemelares en yeguas resultan en pérdidas embrionarias, aborto involuntario en el tercio final o nacimiento de potros debilitados. EI objetivo de esta revisión es analizar el diagnóstico y los métodos de intervención dei embarazo gemelar. EI diagnóstico debe hacerse en Ia fase de migración embrionaria, entre 12 y 15 días. La ruptura de una de las vesículas es el método más efectivo que promueve pérdidas similares a las de un embarazo simple. Si no es posible intervenir en esta etapa, se puede hacer un seguimiento de hasta 30 días para observar la reducción natural. Si no hay reducción, se pueden realizar técnicas de mayor riesgo, que incluyen la aspiración transvaginal, la manipulación craneal del feto, la dislocación craneocervical o la inyección intracardiaco-pulmonar. La eficiencia del manejo está relacionada con la capacidad técnica y elmétodo dei profesional, siempre buscando preservar la integridad de la yegua prenada.

Small intestine strangulating obstruction by mesenteric pedunculated lipoma in a criollo mare

Background: The abdominal lipomas are benign tumors of hyperplastic growth that originate from mesenteric adiposetissue, most commonly in the small intestine. Pedunculated lipoma strangulation occurs when the pedicle wraps aroundan intestinal loop and its mesentery, obstructing the intestinal lumen and blood supply of the affected segment. The aim ofthe present study is to report a case of a Criollo mare presenting a strangulating obstruction of the jejunum and ileum bya pedunculated lipoma, focusing the discussion in the causes and epidemiology of this alteration.Case: A 24-year-old Criollo mare with a body condition score 8 out of 9 was referred to the Veterinary Clinical Hospitalof the Federal University of Pelotas (HCV-UFPel) presenting acute abdominal pain. At the hospital, the mare was presenting mild signs of abdominal discomfort. At the initial clinical exam, the mare had an increased heart and respiratory rates,normal body temperature, hyperemic mucous membranes, capillary refil time of 3 s and absent gut sounds in all fourquadrants during auscultation. Mild dehydration was present, increased fibrinogen and serum lactate. Rectal palpationrevealed distention of the small intestine by gas, during abdominal ultrassonography the small intestinal wall thicknesswas increased Peritoneal lactate was 11 mmol/L and protein was 6 mg/dL. Due to the clinical findings during the exam, anexploratory celiotomy was performed. Necrosis of the final third of jejunum and the entire extension of ileum was found.Examining the compromised intestinal portion, it was observed a linear structure strangulating two segments of the smallintestine. Taking into account the extensive area of irreversible necrosis and the advanced age of the patient, euthanasiawas performed. The mare was then referred for necropsy and findings confirmed...

Peripheral blood markers of sepsis in foals born from mares with experimentally induced ascending placentitis.

BACKGROUND: Neonatal sepsis is a leading cause of neonatal death during the first-week postfoaling. Despite recent advancements in the diagnosis and treatment of sepsis in the newborn foal, the non-specific clinical signs and subtle nature of this disease may result in delayed diagnosis until severe progression of the disease; thus, early detection of sepsis remains critical for a favourable outcome. This study aimed to identify early blood markers as predictive of sepsis on foals. METHODS: Thirty-five foals were allocated into three groups: healthy control foals (n=7) and foals born from mares with placentitis: septic foals (n=9) and non-septic foals (n=19). Blood samples were obtained immediately after foaling and at 12, 24 and 48 hours. All samples were assessed for glucose, lactate, triglycerides, total cholesterol, urea, creatinine, total solids, fibrinogen, gamma-glutamyl transferase (GGT), serum amyloid A (SAA) and alpha-fetoprotein (AFP) concentrations. RESULTS: At foaling, glucose and GGT concentrations were lower in septic foals (P<0.001). Of interest, SAA, AFP, creatinine and total cholesterol were higher in septic foals at parturition (P<0.05). At 12 hours, lactate, triglycerides and total cholesterol concentrations were higher in septic foals. When evaluated at 24 and 48 hours, higher concentrations of SAA and AFP were found in placentitis foals than in the control group. CONCLUSIONS: Total cholesterol and lactate appear to be suitable markers for sepsis during the first 24 hours postpartum. Septic foals displayed altered energy metabolisms as determined by increased triglycerides and cholesterol concentrations, hypoglycaemia at birth and reduced activity of the GGT and increased lactate and urea concentrations. Sepsis was associated with high concentrations of SAA and AFP.

Perfil bioquímico do líquido amniótico de éguas com placentite ascendente e sua relação com parâmetros séricos do potro neonato / Biochemical profile of amniotic fluid in mares with ascending placentitis and its relationship with serum biochemical parameters in neonatal foals

O objetivo do presente estudo foi identificar os parâmetros bioquímicos do líquido amniótico (LA) no momento do parto de éguas com placentite ascendente e relacionar com parâmetros bioquímicos séricos dos neonatos. Foram utilizadas 36 éguas gestantes da raça Puro Sangue Inglês, divididas em dois grupos: placentite (n=5) e sadias (n=31). A placentite foi definida de acordo com a avaliação clínica, exame ultrassonográfico e histopatologia das placentas. Essas éguas foram submetidas a terapia antimicrobiana, antiinflamatórios e altrenogest. A coleta do LA foi realizada na segunda fase do parto e as amostras sanguíneas dos neonatos imediatamente após o nascimento. Foi observada menor concentração de creatinina no LA de éguas com placentite. Potros provenientes de éguas com placentite foram menores em peso e altura ao nascimento, e apresentaram concentrações elevadas de ureia e reduzidas de PPT na avaliação sérica. Foi observado correlação positiva moderada entre creatinina e ureia no LA e correlação positiva fraca entre a creatinina do LA e sérica dos potros. Concluiu-se que o perfil bioquímico do LA e sérico dos neonatos oriundos de éguas com placentite ascendente diferem de gestações sadias. Sendo que esses parâmetros podem ser utilizados como indicadores de alterações placentárias em éguas.(AU)

The aim of this study was to identify the biochemical parameters of amniotic fluid (AF) at parturition in mares with ascending placentitis, and to relate with biochemical parameters in neonatal foals serum. Thirty-six pregnant Thoroughbred mares were divided in 2 groups: mares with placentitis (n=5) and healthy mares (n=31). Placentitis was identified based on clinical signs, ultrasound evaluation and histopathological features in the placenta. Mares with placentitis were treated with antibiotics, antiinflammatories and altrenogest. AF was collected during the second stage of labor and blood samples were taken immediately after foaling. Lower concentrations of creatinine were found in the AF of mares with placentitis. Foals from mares with placentitis showed smaller height and lower birthweight, also showed increase of urea and decrease of total protein levels in blood serum. A moderate positive correlation between urea and creatinine in AF was observed, and a weak positive correlation was found between creatinine in AF and the serum of foals. In conclusion, biochemical composition of AF and of blood serum of neonatal foals from mares with ascending placentitis are different of healthy mares. So, those analysis could be useful to identify placental changes in mares.(AU)

Perfil bioquímico do líquido amniótico de éguas com placentite ascendente e sua relação com parâmetros séricos do potro neonato / Biochemical profile of amniotic fluid in mares with ascending placentitis and its relationship with serum biochemical parameters in neonatal foals

O objetivo do presente estudo foi identificar os parâmetros bioquímicos do líquido amniótico (LA) no momento do parto de éguas com placentite ascendente e relacionar com parâmetros bioquímicos séricos dos neonatos. Foram utilizadas 36 éguas gestantes da raça Puro Sangue Inglês, divididas em dois grupos: placentite (n=5) e sadias (n=31). A placentite foi definida de acordo com a avaliação clínica, exame ultrassonográfico e histopatologia das placentas. Essas éguas foram submetidas a terapia antimicrobiana, antiinflamatórios e altrenogest. A coleta do LA foi realizada na segunda fase do parto e as amostras sanguíneas dos neonatos imediatamente após o nascimento. Foi observada menor concentração de creatinina no LA de éguas com placentite. Potros provenientes de éguas com placentite foram menores em peso e altura ao nascimento, e apresentaram concentrações elevadas de ureia e reduzidas de PPT na avaliação sérica. Foi observado correlação positiva moderada entre creatinina e ureia no LA e correlação positiva fraca entre a creatinina do LA e sérica dos potros. Concluiu-se que o perfil bioquímico do LA e sérico dos neonatos oriundos de éguas com placentite ascendente diferem de gestações sadias. Sendo que esses parâmetros podem ser utilizados como indicadores de alterações placentárias em éguas.

The aim of this study was to identify the biochemical parameters of amniotic fluid (AF) at parturition in mares with ascending placentitis, and to relate with biochemical parameters in neonatal foals’ serum. Thirty-six pregnant Thoroughbred mares were divided in 2 groups: mares with placentitis (n=5) and healthy mares (n=31). Placentitis was identified based on clinical signs, ultrasound evaluation and histopathological features in the placenta. Mares with placentitis were treated with antibiotics, antiinflammatories and altrenogest. AF was collected during the second stage of labor and blood samples were taken immediately after foaling. Lower concentrations of creatinine were found in the AF of mares with placentitis. Foals from mares with placentitis showed smaller height and lower birthweight, also showed increase of urea and decrease of total protein levels in blood serum. A moderate positive correlation between urea and creatinine in AF was observed, and a weak positive correlation was found between creatinine in AF and the serum of foals. In conclusion, biochemical composition of AF and of blood serum of neonatal foals from mares with ascending placentitis are different of healthy mares. So, those analysis could be useful to identify placental changes in mares.

Gestation in a mare with facial deviation (Wry Nose) / Gestação em uma égua com desvio facial (Wry Nose)

Background: Wry nose is a congenital deformity that causes respiratory obstruction and decreased oxygenation rate.Gestation in a wry nose mare may be considered a risk to the neonate since it depends on the maternal environment fordevelopment. Compromised oxygenation during pregnancy can lead to fetal distress and cause consequences on fetaldevelopment. However, depending on the degree of the impairment, the fetus may still be able to adapt. The aim of thepresent study was to report the gestation in a mare with facial deviation until term and to assess blood gases in the mareand neonate, and to evaluate the histomorphometry of the placenta.Case: A Criollo breed mare presenting facial deviation (Wry Nose) was donated to Equine Medicine Research Group(ClinEq) of the Federal University of Pelotas (UFPel) due to the presence of the physical deformity. When the mare wasfive years old, it was inseminated and had a pregnancy confirmed. At the fifth month of gestation, evaluation of fetalaorta diameter, fetal orbital diameter and combined thickness of the uterus and placenta (CTUP) started to be performedmonthly to assess gestation health. The assessment of the fetal orbit and aorta diameter revealed a linear increase of bothvariables with the progress of gestation indicating a normal fetal development. CTUP remained in the normal referencerange, presenting no alterations during the gestational length. The mare foaled at 324 days of gestation a coat showing nocongenital deformities. The foaling was monitored until the complete passage of fetal membranes. A complete clinical andhematological evaluation of the foal was carried out after birth. The foal showed normal adaptive behavior, clinical andhematological parameters during the first hours of life, although presenting physical signs of immaturity. Venous bloodsamples were collected from the mare at 315 days of gestation, immediately after foaling and...(AU)

Immunolocalization of Leptin and its Receptor (ObR-b) in Equine Placenta at Term and Plasma Level Measurement in the Late Gestation.

Placental tissues from humans, rodents, and farm animal contain leptin and its receptor. Expression of leptin has already been described in horses, although there is no description about immunolocalization of leptin and its receptor. The aim of the present study was to investigate the presence and distribution of leptin and ObR-b in the equine placenta at term by immunofluorescence staining, and the changes on plasma leptin concentrations during late gestation. The present study involved eight Criollo-type mares carrying healthy pregnancies. Blood samples were collected during the third trimester of gestation, at foaling, and at 24 hours after foaling. Leptin concentrations were analyzed via radioimmunoassay. Plasma leptin concentrations did not change from the 8th to the 10th month of gestation and displayed a discrete decrease 24 hours after parturition (P = .07). The expression of leptin and ObR-b was observed in the cytoplasm of pseudostratified epithelial cells in the areolar region and in the epithelium of microcotyledons. Also, leptin receptor was allocated in the apical surface of the cells. The presence of leptin and its receptor (ObR-b) in the placenta of mares at term supports an endocrine and autocrine/paracrine action of leptin within this organ.

Gestação gemelar em éguas (parte 1): Desenvolvimento e Fatores de Risco / Twin pregnancies in mares, part I: Development and risk factors / Embarazos gemelares en yeguas, parte l: desarrollo y factores de riesgo

Gestações gemelares em equinos são responsáveis por abortos no terço final de gestação ou nascimento de potros debilitados. O objetivo desta revisão é discutir os fatores de risco para o de gêmeos, sendo mais frequentes em éguas Puro Sangue Inglês, de Polo Argentino e Brasileiro e de Hipismo quando comparadas a Árabes e Pôneis. Éguas solteiras terão maior incidência do múltiplas ovulações e gestações gemelares e éguas mais velhas possuem maiores taxas de múltiplas ovulações, mas não de gêmeos. O uso de indutores de ovulação pode elevar as taxas de ovulações duplas e à medida que a estação reprodutiva progride, aumenta a incidência de gestações gemelares. O conhecimento desses fatores de risco é importante para a realização do diagnóstico e intervenção adequada para evitar prejuízos causados por essas gestações.

Twin pregnancies in horses are responsible for abortion in the late gestation or birth of debilitated foals. The aim of this review is to discuss the risk factors for the development of twin pregnancies. The multiple ovulations are responsible for the occurrence of twins, being more frequent in Thoroughbred, Polo Argentino and Brazilian Sport Horse when compared to Arabs and Ponies. Single mares will have a higher incidence of multiple ovulations and twin pregnancies and older mares have higher rates of multiple ovulations but not twins. The use of ovulation inducers may increase double ovulation rates and as the reproductive season progresses, the incidence of twin pregnancies increases. Knowledge of these risk factors is important for proper diagnosis and intervention to avoid loses caused by this pregnancies.

Los embarazos gemelares en yeguas son pueden causar abortos en la gestación tardía o el nacimiento de potros debilitados. El objetivo de esta revisión es analizar los factores de riesgo para el desarrollo de embarazos gemelares. Las ovulaciones múltiples son responsables por la ocurrencia de gemelos, siendo más frecuentes en yeguas Pura Sangre Inglés, de Polo Argentino y Brasileiro de Hipismo en comparación con las Árabes y Ponis. Las yeguas solteras tendrán una mayor incidencia de ovulaciones múltiples y embarazos gemeiares, y las yeguas mayores tienen tasas más altas de ovulaciones múltiples pero no gemelos. El uso de inductores de la ovulación puede aumentar las tasas de ovulación doble y a medida que avanza la temporada reproductiva aumenta la incidencia de embarazos gemelares. El conocimiento de estos factores de riesgo es importante para un diagnóstico e intervención adecuados para eviar daños causados por estos embarazos.