Zircon U-Pb geochronology and trace element dataset from the Southern Rocky Mountain Volcanic Field, Colorado, USA.

This contribution provides in-situ LA-ICP-MS U-Pb ages and trace element determinations of zircons from dacitic to rhyolitic lavas, ignimbrites and intrusions in the Southern Rocky Mountain Volcanic Field (SRMVF) in Colorado, USA. The data record a period of intense magmatic activity in the Oligocene-early Miocene (∼37-22 Ma) which gave rise to some of the largest explosive ignimbrites in the geological record (e.g. the Fish Canyon Tuff). Age data are drift corrected, but not corrected for radiation dosage or Th disequilibrium, in order to allow users to apply their own algorithms. Xenocrysts (much older crystals up to 2 Ga from the Proterozoic basement) are included in this record.

Interactions of protamine with the marine bacterium, Pseudoalteromonas sp. NCIMB 2021.

UNLABELLED: Pseudoalteromonas sp. NCIMB 2021 (NCIMB 2021) was grown in synthetic seawater (SSW) containing pyruvate, in the presence (SSW(++) ) and absence (SSW(-) ) of divalent cations. Cultures contained single cells. Addition of the cationic antibacterial peptide (CAP), protamine, did not inhibit, but rather increased, the growth of NCIMB 2021 in SSW(++) and caused the bacteria to grow in chains. Bacterial growth was assessed using turbidity, cell counts and the sodium salt of resazurin. In SSW(-) , NCIMB 2021 was no longer resistant to protamine. The minimum inhibitory concentration (MIC) was 5 mg ml(-1) . SIGNIFICANCE AND IMPACT OF THE STUDY: Protamine is a cationic antimicrobial peptide (CAP), which is active against a variety of bacteria. This is the first in-depth study of the interaction of protamine with a marine bacterium, Pseudoalteromonas sp. NCIMB 2021. Our results show that protamine is only active in seawater in the absence of divalent cations. In the presence of the divalent cations, Mg(2+) and Ca(2+) , protamine enhances the growth of Pseudoalteromonas sp. NCIMB 2021 and produces chains rather than individual cells. These are important considerations when deciding on applications for protamine and in terms of understanding its mechanism of action.

Neuropsychological assessment of battered women: a pilot study.

Mean performance of 19 battered women was significantly lower than that of 9 control women on the Halstead-Reitan Neuropsychological Battery, the Wechsler Memory Scale-Revised, and the Quick Neurological Screening Test. 58% of the battered women scored in the impaired range on the Halstead-Reitan Impairment Index; and 53% scored in the impaired range on the Quick Neurological Screening Test. More definitive research was recommended.

A cognitive developmental approach to understanding how children cope with disasters.

TOPIC: This paper applies cognitive developmental theory to explain how preschoolers, school-age children, and adolescents process and respond to disasters. PURPOSE: To help clinicians understand the disaster experience from a child's point of view, recognize age-specific reactions, identify symptoms that may signal coping difficulties, and plan effective interventions. SOURCES: Case examples from the author's work with flood victims illustrate typical reactions for children (preschool to adolescents) within a family context, along with developmentally appropriate interventions. CONCLUSIONS: Children affected by disasters need nursing interventions geared toward their particular developmental level and sensitive to their perception of the disaster.

Human coronavirus 229E infects polarized airway epithelia from the apical surface.

Gene transfer to differentiated airway epithelia with existing viral vectors is very inefficient when they are applied to the apical surface. This largely reflects the polarized distribution of receptors on the basolateral surface. To identify new receptor-ligand interactions that might be used to redirect vectors to the apical surface, we investigated the process of infection of airway epithelial cells by human coronavirus 229E (HCoV-229E), a common cause of respiratory tract infections. Using immunohistochemistry, we found the receptor for HCoV-229E (CD13 or aminopeptidase N) localized mainly to the apical surface of airway epithelia. When HCoV-229E was applied to the apical or basolateral surface of well-differentiated primary cultures of human airway epithelia, infection primarily occurred from the apical side. Similar results were noted when the virus was applied to cultured human tracheal explants. Newly synthesized virions were released mainly to the apical side. Thus, HCoV-229E preferentially infects human airway epithelia from the apical surface. The spike glycoprotein that mediates HCoV-229E binding and fusion to CD13 is a candidate for pseudotyping retroviral envelopes or modifying other viral vectors.

Increasing epithelial junction permeability enhances gene transfer to airway epithelia In vivo.

Gene transfer to airway epithelia is the most direct approach for treating the progressive lung disease associated with cystic fibrosis. However, the transduction efficiency is poor when viral vectors are applied to the mucosal surface. We reported previously that gene transfer via the apical surface of human airway epithelia in vitro was improved by formulating vectors with ethyleneglycol-bis-(2-aminoethyl ether)- N,N,N',N'-tetraacetic acid (EGTA) in a hypotonic buffer. First, we investigated the mechanism for this enhancement. When 100-nm fluorescent beads were applied to the apical surface in the presence of EGTA, paracellular deposition of the particles was noted. Transmission electron microscopy verified that the epithelial junction complex was disrupted under these conditions. The Ca(2+) chelators EGTA, 1,2-bis (2-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid (BAPTA), and ethylenediaminetetraacetic acid all caused a rapid, reversible drop in transepithelial resistance and facilitated gene transfer with retrovirus or adenovirus in vitro. When Ca(2+) chelators were applied to rabbit tracheal epithelia or human nasal epithelia in vivo, the transepithelial voltage decreased, and amiloride sensitivity was lost, suggesting that epithelial junctions opened. Importantly, this novel formulation enhanced both retroviral- and adenoviral-mediated gene transfer to rabbit tracheal epithelia in vivo. This technique may have applications for vector or drug delivery to airway epithelia and other polarized cells.

The cascade model: an alternative to comorbidity in the pathogenesis of posttraumatic stress disorder.

Comorbidity has been used extensively to explain the numerous co-occurring psychiatric syndromes accompanying chronic posttraumatic stress disorder (PTSD). A cascade model is proposed as an alternative to comorbidity for the pathogenesis and clinical course of the condition. This model allows for a dynamic, integrated conceptualization of disease progression in PTSD. Findings in the clinical, epidemiological, neurobiological, and psychosocial literature which might support this model are described. Conceptual and heuristic difficulties and/or potential objections to the model are also examined. Finally, diagnostic and treatment implications as well as potential research applications of the model are discussed.

Dealing with difficult students in the classroom.

Despite the proliferation of literature on dealing with difficult people, little has been written on how to manage problematic students in the classroom. Every teacher encounters difficult students because the classroom is merely a microcosm of the outside world. Nursing educators strive to create an open and caring atmosphere in their classes, but it is still necessary to intervene when students disrupt the class by showing disrespect for others or by getting the discussion off track. The authors describe the most common difficult student roles seen in the classroom and suggest strategies for dealing with them.

The "to feed or not to feed" dilemma.

Advance directives can promote and improve communication between the patient and the provider and ideally safeguard the resident's interests by directing medical care. Participation on the facility's ethics committee allows the dietitian to become involved in the legal and ethical issues regarding feeding and to promote the use of specific advance directives for nutrition and hydration. Consider the goals of patient care carefully and assure that they are individualized and patient-centered. Continually reassess nutritional status and collaborate with the health care team in recommending interventions and care plans for each case.

Should there be a clinical typology of posttraumatic stress disorder?

OBJECTIVE: The current classification of posttraumatic stress disorder in acute, chronic and delayed-onset types is incomplete and of limited usefulness. The present paper explores the possibilities of a clinically-based typology that would reflect both the patients' syndromic presentation and modern research findings. METHOD: Review of current clinical and research literature, with a critical examination of proposed typologies, parameters utilised, applicability and relevance; elaboration of theoretical and practical bases of a clinical typology of posttraumatic stress disorder. RESULTS: None of the existing posttraumatic stress disorder typologies presents a clinically comprehensive scope. A typology supported by literature findings and clinical observations is proposed. The six clinical types are depressive, dissociative, somatomorphic, psychotomorphic, organomorphic and 'neurotic-like'. Substance abuse and personality disorder-like variants remain as areas of investigation. CONCLUSIONS: The proposed typology, while accepting the nuclear manifestations of posttraumatic stress disorder, highlights prominent coexisting symptoms that define the clinical appearance of different patients. The typological approach in posttraumatic stress disorder does not carry the ambiguities of comorbidity, and facilitates a more specific and appropriate management of the cases.

Unique patterns of comorbidity in posttraumatic stress disorder from different sources of trauma.

While there are many studies of comorbidity in combat veterans with posttraumatic stress disorder (PTSD), studies of PTSD from other sources of trauma (e.g., disasters, crimes, and civil violence) are just beginning to emerge. This is the first formal review comparing patterns of comorbidity in PTSD from different sources of trauma. Specific attention is given to the relative frequencies of substance abuse, depression, generalized anxiety, phobic, panic, somatization, psychotic, and personality disorders. The findings reveal that although similarities exist, the comorbidity profiles differ according to the type of trauma experienced and the population studied. Additionally, the evidence suggests that the associated psychiatric disorders are not truly comorbid, but are interwoven with the PTSD.

Medical information: from Surgeon General to superhighway.

Few of those engaged in teaching or research can still be unaware of the existence of the Internet, a global network which has been created by the interconnection of computers in universities, research bodies, commercial organizations, and government departments from all over the World. This network of networks has become know as the 'information superhighway'; it is growing exponentially, acquiring new users by the minute, and expanding, in unexpected and exciting ways, the speed of dissemination, and the format and presentation, of scientific information. This article attempts to put this burgeoning growth in a historical context, and seeks to identify some starting points for Internet/World Wide Web exploration for medical and scientific researchers who wish to harness this uniquely important instrument for their own purposes.

Parallel process in the supervision of child psychotherapy.

Parallel process is a phenomenon in which therapists unconsciously replicate the problems and dynamics of their clients during supervision. It has the unconscious aim of provoking the supervisor to demonstrate how to handle a perplexing situation. Recognizing and resolving parallel process when it occurs is an important supervisory skill. This paper focuses on the unique characteristics of parallel process in child psychotherapy. It reviews various types and manifestations, discusses several case examples, and outlines strategies for intervention.

Effects of extended hospitalization: a one-year follow-up study.

The C. F. Menninger Memorial Hospital is conducting an ongoing follow-up study of the effects of extended hospitalization. The authors report key indicators of outcome at one year postdischarge for 110 patients hospitalized on extended care units for at least 180 days. Outcome is determined by postdischarge rates of rehospitalization, postdischarge suicide attempts, and occupational functioning at the time of the one-year follow-up interview. After comparing these areas with preadmission levels of symptomatology and functioning, the authors report significant improvement in each category at the time of follow-up.

Outcome at discharge for patients in an ongoing follow-up study of hospital treatment.

Methodological and practical difficulties have limited the growth of knowledge about outcome of psychiatric hospital treatment. The authors report on outcome at hospital discharge for 103 long-term and 93 short-term patients treated at the C. F. Menninger Memorial Hospital, part of an ongoing follow-up study of hospital treatment. Discharge outcome is based mainly on ratings of symptoms, global functioning, and therapeutic alliance as well as on patients' reports of satisfaction. At discharge both long- and short-term patients were found to have low levels of symptoms (based on the Brief Psychiatric Rating Scale) and a relatively adequate level of functioning (in the 51-to-60 range on the Global Assessment Scale) and to have been highly satisfied with treatment.

A review of the borderline diagnosis for children.

The concept of the "borderline child" has gained increasing attention in the past 10 years. New diagnostic criteria are being proposed, and research is attempting to identify the salient features of the syndrome. This article examines the childhood borderline concept, reviews some new diagnostic systems, identifies the major diagnostic controversies, lists considerations for differential diagnosis, and discusses implications for practice. The intent is to present the state of the art of the diagnosis and to discuss some potential problems with its use. Unique aspects of the childhood syndrome in contrast to the adult disorder are highlighted. Overlap with other disorders, questions about the continuity of symptoms, lack of attention to developmental variables, and countertransference issues are a few of the problems identified.