Urothelial Malignancy After Normal Hematuria Clinic Investigations: Does Non-visible Hematuria Need Reinvestigation?

OBJECTIVE:  Hematuria is the most common referral to Urology. Most initial evaluations are normal; however there are few medium- to long-term studies about these patients after they are discharged. METHODS:  This study was a retrospective observational case-control study. Patients with normal initial investigations in our hematuria clinic (HC) over a 2-year period in 2012-2013 were included. We reviewed the electronic records of patients choosing January 1, 2021, as our reference date providing a median follow-up of 99 months. The primary aim of this study was to assess the missed urothelial malignancy (UM) rate in this cohort and also the UM rate in those re-referred to the HC. RESULTS:  The study included 573 patients of whom 24.6% (141/573) were re-referred to urology during the study period. The overall missed UM cancer rate was 0.5% and 0.2% died as a result in this follow-up period. The UM cancer rate in those re-referred was 4.3% and of those re-referred with visible hematuria (VH) the UM cancer rate was 5.7%. No patients re-referred with non-visible VH (NVH) were diagnosed with UM. The only urological death during this time was due to UM. CONCLUSION:  All urological malignancy and mortality remain very low even at mediumto long-term follow-up after an initial normal HC investigation. In this study, no patients with recurrent NVH developed UM; therefore, recurrent NVH is unlikely to need reinvestigation. The risk of UM in those re-referred with VH is low but more substantial and warrants reinvestigation, which should include computed tomography urogram imaging.

A Closer Look into White Adipose Tissue Biology and the Molecular Regulation of Stem Cell Commitment and Differentiation.

White adipose tissue (WAT) makes up about 20-25% of total body mass in healthy individuals and is crucial for regulating various metabolic processes, including energy metabolism, endocrine function, immunity, and reproduction. In adipose tissue research, "adipogenesis" is commonly used to refer to the process of adipocyte formation, spanning from stem cell commitment to the development of mature, functional adipocytes. Although, this term should encompass a wide range of processes beyond commitment and differentiation, to also include other stages of adipose tissue development such as hypertrophy, hyperplasia, angiogenesis, macrophage infiltration, polarization, etc.… collectively, referred to herein as the adipogenic cycle. The term "differentiation", conversely, should only be used to refer to the process by which committed stem cells progress through distinct phases of subsequent differentiation. Recognizing this distinction is essential for accurately interpreting research findings on the mechanisms and stages of adipose tissue development and function. In this review, we focus on the molecular regulation of white adipose tissue development, from commitment to terminal differentiation, and examine key functional aspects of WAT that are crucial for normal physiology and systemic metabolic homeostasis.

Reliability, interrelationships, and minimal detectable changes of strength and power metrics among well-trained rugby sevens players.

Despite the importance of strength and power in rugby skills and match outcomes, there exists a noticeable gap in the measurement consistency and estimation of a true change of typical assessments designed to assess these qualities. To address this gap, we investigated the between-session reliability, interrelationships, and minimal detectable changes (MDC) of commonly used strength and power measures in team sports. Sixteen national-level rugby 7 s players were tested on two occasions, one week apart. Both the best and average (of 2-3 trials) peak force, peak power, height, distance, and/or strength indices during countermovement jump (CMJ), drop jump (DJ), isometric mid-thigh pull (IMTP), plyometric push-up (PPU), and standing long jump (SLJ) were obtained. Furthermore, one-repetition maximum (1RM) strength for bench press and back squat, reactive strength index, and dynamic strength index were also determined. Reliability was assessed using intraclass correlation coefficients (ICC) and coefficients of variation (CV), and used for MDC calculations, and interrelationships between variables were determined using correlation coefficients. Reliability was excellent for bench press, back squat, and SLJ (ICCs > 0.91); high to excellent for IMTP peak force, all CMJ, and DJ (except best DJ height and contact time), and PPU peak force parameters (ICCs > 0.78), with < 10% CVs (except PPU peak power). MDCs were generally smaller for average than best values. Large to very large relationships (r = 0.60 to 0.85) were observed between bench press, back squat, and IMTP with selected parameters of CMJ and PPU (p < 0.05), but not in DJ and SLJ. In conclusion, selected measures of strength and power displayed high to excellent reproducibility, with average values (rather than best) offering more stable assessments, and "smaller" MDCs. Based upon the relationships, it can be inferred that maximising strength would likely contribute to enhanced explosive performance.

Routine, Routine, Routine: Sleep Regularity and its Association with Sleep Metrics in Professional Rugby Union Athletes.

BACKGROUND: Maintaining a consistent sleep and wake time is often reported as a key component of circadian rhythmicity and quality sleep. However, the impact of sleep onset and offset time variability on overall sleep outcomes are underreported in elite athlete populations. This study investigated the relationship between sleep onset and offset time variability using the sleep regularity index (SRI) and measures of sleep and well-being in professional rugby union athletes. Twenty-three professional male rugby union athletes (mean ± SD, age: 23 ± 3 y) underwent sleep monitoring via wrist actigraphy for three weeks during a pre-season phase of training and completed a daily wellness questionnaire. Median SRI was calculated and used to stratify the trainees into two quantile groups: >76.4 SRI (Regular, n = 11) and < 76.4 SRI (Irregular, n = 12). RESULTS: The regular sleep group showed significantly longer total sleep duration (p = 0.02, d = 0.97) compared to the irregular group (7:42 ± 0:29 vs. 7:18 ± 0:20 h: min per night, respectively). Furthermore, while not statistically significant, the regular sleep group showed greater sleep efficiency and less wake episodes compared to irregular sleepers, as demonstrated by moderate effect sizes (d = 0.71 and 0.69, respectively). CONCLUSIONS: The results from this study indicate that minimizing variability in sleep onset and offset time is beneficial for increasing sleep duration and may improve sleep efficiency during pre-season training in elite male rugby union athletes. This study provides evidence for the importance of including sleep-wake routines as a key component of sleep education interventions.

Unveiling the Role of Oxidative Stress in Cochlear Hair Cell Death: Prospective Phytochemical Therapeutics against Sensorineural Hearing Loss.

Hearing loss represents a multifaceted and pervasive challenge that deeply impacts various aspects of an individual's life, spanning psychological, emotional, social, and economic realms. Understanding the molecular underpinnings that orchestrate hearing loss remains paramount in the quest for effective therapeutic strategies. This review aims to expound upon the physiological, biochemical, and molecular aspects of hearing loss, with a specific focus on its correlation with diabetes. Within this context, phytochemicals have surfaced as prospective contenders in the pursuit of potential adjuvant therapies. These compounds exhibit noteworthy antioxidant and anti-inflammatory properties, which hold the potential to counteract the detrimental effects induced by oxidative stress and inflammation-prominent contributors to hearing impairment. Furthermore, this review offers an up-to-date exploration of the diverse molecular pathways modulated by these compounds. However, the dynamic landscape of their efficacy warrants recognition as an ongoing investigative topic, inherently contingent upon specific experimental models. Ultimately, to ascertain the genuine potential of phytochemicals as agents in hearing loss treatment, a comprehensive grasp of the molecular mechanisms at play, coupled with rigorous clinical investigations, stands as an imperative quest.

A comparison of three different work to rest periods during intermittent sprint training on maintaining sprint effort performance.

Background/objectives: Team sports are characterised by repeated maximal intensity bursts of activity, requiring significant energy contribution from the phosphagen pathways. The objective of this study was to evaluate the impact of different rest periods on repeated maximal intensity efforts. Methods: The effect of three different recovery periods (60 s, 90 s and 120 s) during a 10 × six-seconds intermittent sprint training protocol performed on a cycle ergometer was investigated. Thirteen part-time female athletes from two sports, Rugby Sevens and Netball competing for their state participated in the study. Peak Power (PPO), Mean Power (MPO), "total work" in the form of calorie expenditure, performance decrement, repetitions over 95% PPO, blood lactate, and RPE were recorded. Results: There was a significant effect of condition on MPO and calorie expenditure (p < 0.050). MPO was significantly lower for 60 s compared to 90 s (710.4 vs 734.4 W, ES = 0.27-0.42) and 120 s (710.4 vs 743.3 W, ES = 0.36-0.47). Calorie expenditure was significantly lower for 60 s compared to 90 s (4.41 vs 4.56 cal, ES = 0.25-0.46) and 120 s (4.41 vs 4.59 cal, ES = 0.40-0.48). There was a significant effect of time (60 s 11.7, 90 s 11.1.120 s 10.9 mmol/L, p < 0.010) but not condition (p = 0.617) for blood lactate accumulation, and a significant difference in session RPE between 60 and both 90 s and 120 s (60 s 15.5, 90 s 14.2. p = 0.034 120 s 13.9, p = 0.039). Conclusion: Shorter recovery durations resulted in decreased mean power and calorie expenditure, but higher RPE when compared to longer recovery periods. All three recovery periods may have fallen between the fast and slow phases of PCr resynthesis of approximately 20 and 180 s resulting in partial but not complete recovery. Total training time should be a consideration when determining what protocol to implement.

The Development of a Resting Metabolic Rate Prediction Equation for Professional Male Rugby Union Players.

Determining resting metabolic rate (RMR) is an important aspect when calculating energy requirements for professional rugby union players. Prediction equations are often used for convenience to estimate RMR. However, the accuracy of current prediction equations for professional rugby union players remains unclear. The aims of this study were to examine the RMR of professional male rugby union players compared to nine commonly used prediction equations and develop and validate RMR prediction equations specific to professional male rugby union players. One hundred and eight players (body mass (BM) = 102.9 ± 13.3 kg; fat-free mass (FFM) = 84.8 ± 10.2 kg) undertook Dual-energy X-ray Absorptiometry scans to assess body composition and indirect calorimetry to determine RMR. Mean RMR values of 2585 ± 176 kcal∙day-1 were observed among the group with forwards (2706 ± 94 kcal·day-1), demonstrating significantly (p < 0.01; d = 1.93) higher RMR compared to backs (2465 ± 156 kcal·day-1), which appeared to be due to their higher BM and FFM measures. Compared to the measured RMR for the group, seven of the nine commonly used prediction equations significantly (p < 0.05) under-estimated RMR (-104-346 kcal·day-1), and one equation significantly (p < 0.01) over-estimated RMR (192 kcal·day-1). This led to the development of a new prediction equation using stepwise linear regression, which determined that the strongest predictor of RMR for this group was FFM alone (R2 = 0.70; SEE = 96.65), followed by BM alone (R2 = 0.65; SEE = 104.97). Measuring RMR within a group of professional male rugby union players is important, as current prediction equations may under- or over-estimate RMR. If direct measures of RMR cannot be obtained, we propose the newly developed prediction equations be used to estimate RMR within professional male rugby union players. Otherwise, developing team- and/or group-specific prediction equations is encouraged.

The relationship between cardiorespiratory fitness and blood pressure among airline pilots: a mediation analysis of body composition.

OBJECTIVE: Blood pressure (BP), cardiorespiratory fitness (CRF), and body composition are independently associated with health outcomes, yet the relationship between these variables has not been explored among airline pilots. The aim of this study was to evaluate the relationship between CRF and BP, and further examine whether the relationship is mediated by body composition. METHODS: A cross-sectional study was conducted among 356 airline pilots in New Zealand. We measured height, body mass, BP, waist circumference, skinfolds, and CRF (via a WattBike cycle ergometer submaximal VO 2max test). Partial correlation coefficients were estimated to examine the relationships between all variables while controlling for age and sex. Haye's PROCESS macro and the Sobel test were utilized for the mediation analysis. RESULTS: All body composition variables (body mass index, waist circumference and body fat percentage) were positively correlated with all BP variables (systolic pressure, diastolic pressure and mean arterial pressure) ( P  < 0.001). CRF was negatively correlated with all body composition and BP variables ( P  < 0.001). The Sobel test and indirect effect were significant ( P  < 0.001), confirming that all body composition variables partially mediate the relationship between CRF and all blood pressure variables. CONCLUSION: Lower CRF is associated with higher blood pressure, and body composition partially mediates the relationship between these health risk factors. These findings highlight the importance of physical fitness and healthy body composition in the management of blood pressure among this occupational group.

Eccentric Force-Velocity-Load Relationship in Trained Rugby Union Athletes.

ABSTRACT: McNeill, C, Beaven, CM, McMaster, DT, Ward, P, and Gill, N. Eccentric force-velocity-load relationship in trained rugby union athletes. J Strength Cond Res 38(3): 549-555, 2024-The force-velocity relationship is traditionally believed to resemble a hyperbolic shape, known as the "force-velocity curve." However, there is less evidence regarding this relationship during eccentric muscle action in multijoint isotonic exercise, especially in applied settings. The purpose of this study was to investigate the force-velocity-load relationship in an incremental eccentric back squat test. In addition, 37 professional male rugby union athletes were recruited to participate. Separate generalized linear mixed models were used to analyze the effect of barbell load on relative eccentric peak force (REPF), relative eccentric mean force (REMF), eccentric peak velocity (EPV), and eccentric mean velocity (EMV). A significant effect of load ( p < 0.05) was observed for each of the eccentric variables tested. Each increase in barbell load tended to result in a linear increase in REMF and a decrease in EMV and EPV; however, we observed a plateauing effect for REPF as load increased. These results show that for "peak" variables lighter loads produced similar magnitudes of force, but generally moved at higher velocities than heavier loads. These observations suggest that the eccentric force-velocity-load relationship may vary depending on the parameters used. Quantifying rapid, multijoint eccentric performance is justified as it seems to provide valuable insight into individual athletic capability and training program design. Further research may investigate the responsiveness of the qualities to training and the causal nature of eccentric characteristics and athletic performance.

Kinetic Analysis, Potentiation, and Fatigue During Vertical and Horizontal Plyometric Training: An In-Depth Investigation Into Session Volume.

Despite previous support for plyometric training, optimal dosing strategies remain unclear. PURPOSE: To investigate vertical and horizontal jump kinetic performance following a low-volume plyometric stimulus with progressively increased session jump volume. METHODS: Sixteen academy rugby players (20.0 [2.0] y; 103.0 [17.6] kg; 184.3 [5.5] cm) volunteered for this study. Vertical and horizontal jump sessions were conducted 1 week apart and consisted of a 40-jump low-volume plyometric stimulus using 4 exercises, after which volume was progressively increased to 200 jumps, using countermovement jump (CMJ) for vertical sessions and horizontal broad jump (HBJ) for horizontal sessions. Jump performance was assessed via force-plate analysis at baseline (PRE-0), following the low-volume plyometric stimulus (P-40), and every subsequent 10 jumps until the end of the session (P-50, P-60, P-70, ... P-200). RESULTS: The low-volume stimulus was effective in potentiating HBJ (2% to 5%) but not CMJ (0% to -7%) performance (P < .001). The HBJ performance enhancements were maintained throughout the entire high-volume session, while CMJ realized small but significant decrements (-5% to -7%) in jump height P-50 to P-80 before recovering to presession values. Moreover, increases in eccentric impulse (5% to 24%; P < .001) in both sessions were associated with decreased or maintained concentric impulse, indicating a breakdown in performance-augmenting mechanisms and less effective power transfer concentrically after moderate volumes. CONCLUSION: Practitioners should consider kinetic differences between HBJ and CMJ with increasing volume to better inform and understand session dosing strategies.

Automatic-Scoring Actigraph Compares Favourably to a Manually-Scored Actigraph for Sleep Measurement in Healthy Adults.

Introduction Actigraphy has been used widely in sleep research due to its non-invasive, cost-effective ability to monitor sleep. Traditionally, manually-scored actigraphy has been deemed the most appropriate in the research setting; however, technological advances have seen the emergence of automatic-scoring wearable devices and software. Methods A total of 60-nights of sleep data from 20-healthy adult participants (10 male, 10 female, age: 26 ± 10 years) were collected while wearing two devices concomitantly. The objective was to compare an automatic-scoring device (Fatigue Science Readiband™ [AUTO]) and a manually-scored device (Micro Motionlogger® [MAN]) based on the Cole-Kripke method. Manual-scoring involved trained technicians scoring all 60-nights of sleep data. Sleep indices including total sleep time (TST), total time in bed (TIB), sleep onset latency (SOL), sleep efficiency (SE), wake after sleep onset (WASO), wake episodes per night (WE), sleep onset time (SOT) and wake time (WT) were assessed between the two devices using mean differences, 95% levels of agreement, Pearson-correlation coefficients ( r ), and typical error of measurement (TEM) analysis. Results There were no significant differences between devices for any of the measured sleep variables ( p ≥0.05). All sleep indices resulted in very-strong correlations ( all r ≥0.84) between devices. A mean difference between devices of <1 minutes for TST was associated with a TEM of 15.5 minute (95% CI =12.3 to 17.7 minutes). Conclusion Given there were no significant differences between devices in the current study, automatic-scoring actigraphy devices may provide a more practical and cost-effective alternative to manually-scored actigraphy in healthy populations.

The associations between physical-test performance and match performance in women's Rugby Sevens players.

Evaluating the relationships between physical-test and match performance in team sports could be useful for training prescription and athlete evaluation. Here we investigated these relationships in women's Rugby Sevens. Thirty provincial-representative players performed Bronco-fitness, countermovement-jump, acceleration, speed, and strength tests within two weeks before a two-day tournament. Match-running and match-action performance measures were provided by GPS units and video analysis. Generalised and general linear mixed models were employed to estimate the effect of a two standard-deviation difference in physical-test measures on match measures. Effect magnitudes were assessed via standardisation (using the between-player SD) and, for effects on tries scored, also via match winning (based on simulating matches). Evidence for substantial and trivial true magnitudes was provided by one-sided interval-hypothesis tests and Bayesian analysis. There was good evidence of positive effects of many physical-test measures on match high-intensity running, with large effects for jump height and acceleration. There was some evidence of small-moderate positive effects of speed and Bronco, and of small-moderate negative effects of maximal strength and jump height, on match total running and high intensity changes in speed. The evidence was generally inadequate for associations between physical-test measures and match actions, but there was good evidence of small-large positive effects of back squat and jump height on tries scored. Enhancing players' jump height and back-squat performance might therefore increase the likelihood of match success in women's Rugby Sevens.

Daytime naps improve afternoon power and perceptual measures in elite rugby union athletes-a randomized cross-over trial.

Daytime naps are used by elite athletes in both training and match-day settings. Currently, there are limited interventional studies on the efficacy of napping on physical performance in elite team-sport athletes. Therefore, the objective was to investigate the effect of a daytime nap (<1 hour) on afternoon performance of peak power, reaction time, self-reported wellness, and aerobic performance in professional rugby union athletes. A randomized cross-over design was carried out among 15 professional rugby union athletes. Athletes performed nap (NAP) and no nap (CON) conditions on two occasions, separated by 1 week. Baseline testing of reaction time, self-reported wellness, and a 6-second peak power test on a cycle ergometer were completed in the morning, followed by 2 × 45-minute training sessions, after which athletes completed the NAP or CON condition at 1200 hours. Following the nap period, baseline measures were retested in addition to a 30-minute fixed-intensity interval cycle and a 4-minute maximal effort cycling test. A significant group × time interaction was determined for 6-second peak power output (+157.6 W, p < 0.01, d = 1.53), perceived fatigue (-0.2 AU, p = 0.01, d = 0.37), and muscle soreness (-0.1 AU, p = 0.04, d = 0.75) in favor of the NAP condition. A significantly lower perceived exertion rating (-1.2 AU, p < 0.01, d = 1.72) was recorded for the fixed-intensity session in favor of NAP. This study highlights that utilizing daytime naps between training sessions on the same day improved afternoon peak power and lowered perceptions of fatigue, soreness, and exertion during afternoon training in professional rugby union athletes.

Healthy Nutrition, Physical Activity, and Sleep Hygiene to Promote Cardiometabolic Health of Airline Pilots: A Narrative Review.

Background: Airline pilots experience unique occupational demands that may contribute to adverse physical and psychological health outcomes. Epidemiological reports have shown a substantial prevalence of cardiometabolic health risk factors including excessive body weight, elevated blood pressure, poor lifestyle behaviors, and psychological fatigue. Achieving health guidelines for lifestyle behavior nutrition, physical activity, and sleep are protective factors against the development of noncommunicable diseases and may mitigate the unfavorable occupational demands of airline pilots. This narrative review examines occupational characteristics for sleep, nutrition, and physical activity and outlines evidence-based strategies to inform health behavior interventions to mitigate cardiometabolic health risk factors among airline pilots. Methods: Literature sources published between 1990 and 2022 were identified through electronic searches in PubMed, MEDLINE (via OvidSP), PsychINFO, Web of Science, and Google Scholar databases, and a review of official reports and documents from regulatory authorities pertaining to aviation medicine and public health was conducted. The literature search strategy comprised key search terms relating to airline pilots, health behaviors, and cardiometabolic health. The inclusion criteria for literature sources were peer-reviewed human studies, meta-analyses, systematic reviews, and reports or documents published by regulatory bodies. Results: The results of the review show occupational factors influencing nutrition, sleep, and physical activity behaviors and delineate evident occupational disruptions to these lifestyle behaviors. Evidence from clinical trials demonstrates the efficacy of nutrition, sleep, and physical activity interventions for enhancing the cardiometabolic health of airline pilots. Conclusion: This narrative review suggests that implementing evidence-based interventions focused on nutrition, physical activity, and sleep could help mitigate cardiometabolic health risk factors among airline pilots, who are particularly susceptible to adverse health outcomes due to unique occupational demands.

New Zealand Youth Rugby Sevens: A Comparative Match Demands Study.

Rugby sevens has established itself on the world stage since its inclusion in the 2016 Olympics. Participation among New Zealand (NZ) youth has surged. Sevens games have specific high demands, but little is known about these competitive demands in regards to youth. Two NZ male youth squads (U15, n = 13; U19, n = 14) were monitored during a national sevens tournament. Microsensor technology captured heart rate (HR) and kinematic performance. The rating of perceived exertion (RPE) was collected for U15 matches only. U19 and U15 players ran 108 ± 11 and 116 ± 13 m·min-1 at an average speed (VAVG) of 6.5 ± 0.6 and 6.9 ± 0.8 km·h-1. Peak speeds (VPEAK) reached 33.7 km·h-1, and high-intensity running distance (HIRD) averaged 252 ± 102 m. U15 (44.3 ± 9.2 game-1) and U19 (39.4 ± 6.1 game-1) showed different sprint rates. U15 covered more moderate-velocity distance (20-80% VMAX) and less low-velocity distance (<20% VMAX). RPE was 13 ± 1 (U15). An average HR of 90.0 ± 3.9% HRMAX was recorded. Upwards of 57% of game time was played at >95% HRMAX. Youth sevens competition is specifically demanding. U15 can experience greater loads than older peers in rugby. Coaches can use this information to optimize players' physical development.

Ginsenoside Rc from Panax Ginseng Ameliorates Palmitate-Induced UB/OC-2 Cochlear Cell Injury.

By 2050, at least 700 million people will require hearing therapy while 2.5 billion are projected to suffer from hearing loss. Sensorineural hearing loss (SNHL) arises from the inability of the inner ear to convert fluid waves into neural electric signals because of injury to cochlear hair cells that has resulted in their death. In addition, systemic chronic inflammation implicated in other pathologies may exacerbate cell death leading to SNHL. Phytochemicals have emerged as a possible solution because of the growing evidence of their anti-inflammatory, antioxidant, and anti-apoptotic properties. Ginseng and its bioactive molecules, ginsenosides, exhibit effects that suppress pro-inflammatory signaling and protect against apoptosis. In the current study, we investigated the effects of ginsenoside Rc (G-Rc) on UB/OC-2 primary murine sensory hair cell survival in response to palmitate-induced injury. G-Rc promoted UB/OC-2 cell survival and cell cycle progression. Additionally, G-Rc enhanced the differentiation of UB/OC-2 cells into functional sensory hair cells and alleviated palmitate-induced inflammation, endoplasmic reticulum stress, and apoptosis. The current study offers novel insights into the effects of G-Rc as a potential adjuvant for SNHL and warrants further studies elucidating the molecular mechanisms.

Operation early-bird: Investigating altered light exposure in military barracks on sleep and performance-a placebo-controlled study.

The manipulation of light exposure in the evening has been shown to modulate sleep, and may be beneficial in a military setting where sleep is reported to be problematic. This study investigated the efficacy of low-temperature lighting on objective sleep measures and physical performance in military trainees. Sixty-four officer-trainees (52 male/12 female, mean ± SD age: 25 ± 5 years) wore wrist-actigraphs for 6 weeks during military training to quantify sleep metrics. Trainee 2.4-km run time and upper-body muscular-endurance were assessed before and after the training course. Participants were randomly assigned to either: low-temperature lighting (LOW, n = 19), standard-temperature lighting with a placebo "sleep-enhancing" device (PLA, n = 17), or standard-temperature lighting (CON, n = 28) groups in their military barracks for the duration of the course. Repeated-measures ANOVAs were run to identify significant differences with post hoc analyses and effect size calculations performed where indicated. No significant interaction effect was observed for the sleep metrics; however, there was a significant effect of time for average sleep duration, and small benefits of LOW when compared with CON (d = 0.41-0.44). A significant interaction was observed for the 2.4-km run, with the improvement in LOW (Δ92.3 s) associated with a large improvement when compared with CON (Δ35.9 s; p = 0.003; d = 0.95 ± 0.60), but not PLA (Δ68.6 s). Similarly, curl-up improvement resulted in a moderate effect in favour of LOW (Δ14 repetitions) compared with CON (Δ6; p = 0.063; d = 0.68 ± 0.72). Chronic exposure to low-temperature lighting was associated with benefits to aerobic fitness across a 6-week training period, with minimal effects on sleep measures.

A global perspective on collision and non-collision match characteristics in male rugby union: Comparisons by age and playing standard.

This study quantified and compared the collision and non-collision match characteristics across age categories (i.e. U12, U14, U16, U18, Senior) for both amateur and elite playing standards from Tier 1 rugby union nations (i.e. England, South Africa, New Zealand). Two-hundred and one male matches (5911 min ball-in-play) were coded using computerised notational analysis, including 193,708 match characteristics (e.g. 83,688 collisions, 33,052 tackles, 13,299 rucks, 1006 mauls, 2681 scrums, 2923 lineouts, 44,879 passes, 5568 kicks). Generalised linear mixed models with post-hoc comparisons and cluster analysis compared the match characteristics by age category and playing standard. Overall significant differences (p < 0.001) between age category and playing standard were found for the frequency of match characteristics, and tackle and ruck activity. The frequency of characteristics increased with age category and playing standard except for scrums and tries that were the lowest at the senior level. For the tackle, the percentage of successful tackles, frequency of active shoulder, sequential and simultaneous tackles increased with age and playing standard. For ruck activity, the number of attackers and defenders were lower in U18 and senior than younger age categories. Cluster analysis demonstrated clear differences in all and collision match characteristics and activity by age category and playing standard. These findings provide the most comprehensive quantification and comparison of collision and non-collision activity in rugby union demonstrating increased frequency and type of collision activity with increasing age and playing standard. These findings have implications for policy to ensure the safe development of rugby union players throughout the world.

The safety of rugby union, especially the tackle, has previously been questioned but limited data are available to understand the collision and non-collision match characteristics between different age categories and playing standards.The frequency of collision and non-collision match characteristics increase with age and playing standard except for the frequency of scrums and tries which are lowest at the Senior Elite level. The activity of the tackle and ruck are also different between age categories and playing standards.Hierarchical cluster analysis demonstrated clear differences in all and collision match characteristics between junior (i.e. U12, U14, U16), and amateur (i.e. U18 and senior) and elite (i.e. U18 and senior) playing levels.Governing bodies and practitioners should be aware of the differences in collision and non-collision match characteristics by age and playing standard, when reviewing future versions of rugby union.

Additive energetic contributions of multiple peptide positions determine the relative promiscuity of viral and human sequences for PDZ domain targets.

Protein-protein interactions that involve recognition of short peptides are critical in cellular processes. Protein-peptide interaction surface areas are relatively small and shallow, and there are often overlapping specificities in families of peptide-binding domains. Therefore, dissecting selectivity determinants can be challenging. PDZ domains are a family of peptide-binding domains located in several intracellular signaling and trafficking pathways. These domains are also directly targeted by pathogens, and a hallmark of many oncogenic viral proteins is a PDZ-binding motif. However, amidst sequences that target PDZ domains, there is a wide spectrum in relative promiscuity. For example, the viral HPV16 E6 oncoprotein recognizes over double the number of PDZ domain-containing proteins as the cystic fibrosis transmembrane conductance regulator (CFTR) in the cell, despite similar PDZ targeting-sequences and identical motif residues. Here, we determine binding affinities for PDZ domains known to bind either HPV16 E6 alone or both CFTR and HPV16 E6, using peptides matching WT and hybrid sequences. We also use energy minimization to model PDZ-peptide complexes and use sequence analyses to investigate this difference. We find that while the majority of single mutations had marginal effects on overall affinity, the additive effect on the free energy of binding accurately describes the selectivity observed. Taken together, our results describe how complex and differing PDZ interactomes can be programmed in the cell.

A Contactless App-Based Intervention to Improve Health Behaviors in Airline Pilots: A Randomized Trial.

INTRODUCTION: There is a need for enhanced preventive health care among airline pilots to mitigate the prevalence of cardiometabolic health risk factors. STUDY DESIGN: A randomized, waitlist-controlled trial was utilized to evaluate the effectiveness of a smartphone-based app intervention for improving health behaviors and cardiometabolic health parameters. SETTING/PARTICIPANTS: A total of 186 airline pilots (aged 43.2±9.1 years; male, 64%) were recruited and participated in the trial during 2022. INTERVENTION: This intervention was a personalized, 16-week smartphone-based app multicomponent physical activity, healthy eating, and sleep hygiene intervention. MAIN OUTCOME MEASURES: Outcome measures of objective health (Cooper's 12-minute exercise test, resting heart rate, push ups, plank isometric hold, body mass), subjective health (self-rated health, perceived psychological stress and fatigue), and health behaviors (weekly physical activity, sleep quality and duration, fruit and vegetable intake) were collected at baseline and after intervention. The waitlist control completed the same measures. RESULTS: Significant interactions for time Χ group from baseline to 16 weeks were found for all outcome measures (p<0.001). Significant between-group differences for positive health changes in favor of the intervention group were found after intervention for all outcome measures (p<0.05, d=0.4-1.0) except for self-rated health, body mass, and Pittsburgh Sleep Quality Index score. CONCLUSIONS: Study findings show that an app-based health behavior intervention can elicit positive cardiometabolic health changes among airline pilots over 16 weeks, associated with trivial to large effect sizes. TRIAL REGISTRATION: The trial protocol was prospectively registered at The Australian New Zealand Clinical Trials Registry (ACTRN12622000288729).