RÉSUMÉ
Arboviruses are an emerging threat to public health. Arbovirus transmission to vertebrates hinges on dissemination from the arthropod gastrointestinal tract, and ultimately infection of the arthropod salivary glands. Therefore, salivary gland immunity impacts arbovirus transmission; however, these immune responses are poorly understood. Here, we describe the utility of Drosophila melanogaster as a salivary gland infection model. First, we describe the use of a salivary gland-specific driver to launch RNA interference or virus replicon transgenes. Next, we infect flies with an arbovirus panel and find multiple viruses that infect Drosophila salivary glands, albeit inefficiently. We find that this infection is not controlled by antiviral RNA silencing; thus, we silence a panel of immune genes in the salivary glands, but do not observe changes in infection. These data suggest that Drosophila may be used to study salivary gland infection, and that there are likely unexplored pathways controlling infection of this tissue.
Sujet(s)
Arbovirus , Drosophila melanogaster , Modèles animaux , Animaux , Animal génétiquement modifié , Protéines Argonaute/génétique , Protéines Argonaute/métabolisme , Protéines de Drosophila/génétique , Protéines de Drosophila/métabolisme , Femelle , Interactions hôte-pathogène , Immunité innée , Interférence par ARN , Glandes salivaires/immunologie , Glandes salivaires/métabolisme , Glandes salivaires/virologie , Transduction du signal/génétique , Transduction du signal/immunologie , Vesiculovirus , Réplication virale , Virus ZikaRÉSUMÉ
Arthropod-borne viruses are diverse pathogens and are often associated with human disease. These viruses span multiple genera, including flaviviruses, alphaviruses, and bunyaviruses. In a high-throughput drug screen, we found that tenovin-1 was antiviral against the flaviviruses Zika virus and dengue virus. Tenovin-1 is a sirtuin inhibitor, and here we found that inhibition of sirtuins, but not inhibition of the related histone deacetylases, is potently antiviral against diverse arboviruses. Sirtuin inhibitors block infection of arboviruses in multiple human cell types. We found that sirtuin inhibitors arrest infection downstream of entry but that they do so at an early step, preventing the accumulation of viral RNA and protein. However, sirtuin inhibitors had no impact on the replication of flaviviral replicons, suggesting a defect in the establishment of replication. Consistent with this, we found that sirtuin inhibitors impacted double-stranded RNA (dsRNA) accumulation during flaviviral infection. Since these viruses infect vector insects, we also tested whether sirtuin inhibitors impacted infection of adult flies and found that these inhibitors blocked infection; therefore, they target highly conserved facets of replication. Taken together, these results suggest that sirtuin inhibitors represent a new class of potent host-targeting antivirals.IMPORTANCE Arthropod-borne viruses are diverse pathogens and are associated with human disease. Through high-throughput drug screening, we found that sirtuin inhibitors are potently antiviral against diverse arboviruses, including flaviviruses such as West Nile virus, bunyaviruses such as Rift Valley fever virus, and alphaviruses such as chikungunya virus. Sirtuin inhibitors block infection of these viruses in multiple human cell types. Moreover, we found that sirtuin inhibitors arrest infection downstream of entry but that they do so at an early step, preventing the accumulation of viral RNA and protein. Since these viruses infect vector insects, we also tested whether sirtuin inhibitors impacted infection of adult flies and found that these inhibitors blocked infection; therefore, they target highly conserved facets of replication. Taken together, these results suggest that sirtuin inhibitors represent a new class of potent host-targeting antivirals.
Sujet(s)
Acétanilides/pharmacologie , Antiviraux/pharmacologie , Arbovirus/effets des médicaments et des substances chimiques , Diptera/virologie , Interactions hôte-microbes/effets des médicaments et des substances chimiques , Sirtuines/antagonistes et inhibiteurs , Thiourée/analogues et dérivés , Animaux , Virus de la dengue/effets des médicaments et des substances chimiques , Diptera/effets des médicaments et des substances chimiques , Découverte de médicament , Femelle , Cellules HEK293 , Tests de criblage à haut débit , Humains , Thiourée/pharmacologie , Réplication virale/effets des médicaments et des substances chimiques , Virus Zika/effets des médicaments et des substances chimiquesRÉSUMÉ
The emerging arthropod-borne flavivirus Zika virus (ZIKV) is associated with neurological complications. Innate immunity is essential for the control of virus infection, but the innate immune mechanisms that impact viral infection of neurons remain poorly defined. Using the genetically tractable Drosophila system, we show that ZIKV infection of the adult fly brain leads to NF-kB-dependent inflammatory signaling, which serves to limit infection. ZIKV-dependent NF-kB activation induces the expression of Drosophila stimulator of interferon genes (dSTING) in the brain. dSTING protects against ZIKV by inducing autophagy in the brain. Loss of autophagy leads to increased ZIKV infection of the brain and death of the infected fly, while pharmacological activation of autophagy is protective. These data suggest an essential role for an inflammation-dependent STING pathway in the control of neuronal infection and a conserved role for STING in antimicrobial autophagy, which may represent an ancestral function for this essential innate immune sensor.
Sujet(s)
Autophagie/physiologie , Encéphale/immunologie , Drosophila melanogaster/immunologie , Immunité innée , Inflammation/immunologie , Transduction du signal/immunologie , Infection par le virus Zika/immunologie , Animaux , Anti-infectieux , Encéphale/virologie , Lignée cellulaire , Chlorocebus aethiops , Modèles animaux de maladie humaine , Drosophila melanogaster/génétique , Drosophila melanogaster/virologie , Encéphalite/immunologie , Encéphalite/virologie , Femelle , Humains , Mâle , Facteur de transcription NF-kappa B/immunologie , Neurones/immunologie , Neurones/virologie , Interférence par ARN/immunologie , Cellules Vero , Virus Zika/pathogénicitéRÉSUMÉ
RNA degradation is tightly regulated to selectively target aberrant RNAs, including viral RNA, but this regulation is incompletely understood. Through RNAi screening in Drosophila cells, we identified the 3'-to-5' RNA exosome and two components of the exosome cofactor TRAMP (Trf4/5-Air1/2-Mtr4 polyadenylation) complex, dMtr4 and dZcchc7, as antiviral against a panel of RNA viruses. We extended our studies to human orthologs and found that the exosome as well as TRAMP components hMTR4 and hZCCHC7 are antiviral. While hMTR4 and hZCCHC7 are normally nuclear, infection by cytoplasmic RNA viruses induces their export, forming a cytoplasmic complex that specifically recognizes and induces degradation of viral mRNAs. Furthermore, the 3' untranslated region (UTR) of bunyaviral mRNA is sufficient to confer virus-induced exosomal degradation. Altogether, our results reveal that signals from viral infection repurpose TRAMP components to a cytoplasmic surveillance role where they selectively engage viral RNAs for degradation to restrict a broad range of viruses.
Sujet(s)
Exosomes/métabolisme , Stabilité de l'ARN/physiologie , ARN viral/métabolisme , Animaux , Lignée cellulaire , Cytoplasme/métabolisme , Drosophila/virologie , Humains , Complexes multiprotéiques/génétique , Polyadénylation , Liaison aux protéines , Transport des protéines , Interférence par ARN , Infections à virus à ARN/métabolisme , Infections à virus à ARN/virologie , Virus à ARN/physiologie , Facteurs de transcription/métabolismeRÉSUMÉ
A unique facet of arthropod-borne virus (arbovirus) infection is that the pathogens are orally acquired by an insect vector during the taking of a blood meal, which directly links nutrient acquisition and pathogen challenge. We show that the nutrient responsive ERK pathway is both induced by and restricts disparate arboviruses in Drosophila intestines, providing insight into the molecular determinants of the antiviral "midgut barrier." Wild-type flies are refractory to oral infection by arboviruses, including Sindbis virus and vesicular stomatitis virus, but this innate restriction can be overcome chemically by oral administration of an ERK pathway inhibitor or genetically via the specific loss of ERK in Drosophila intestinal epithelial cells. In addition, we found that vertebrate insulin, which activates ERK in the mosquito gut during a blood meal, restricts viral infection in Drosophila cells and against viral invasion of the insect gut epithelium. We find that ERK's antiviral signaling activity is likely conserved in Aedes mosquitoes, because genetic or pharmacologic manipulation of the ERK pathway affects viral infection of mosquito cells. These studies demonstrate that ERK signaling has a broadly antiviral role in insects and suggest that insects take advantage of cross-species signals in the meal to trigger antiviral immunity.
Sujet(s)
Arbovirus/immunologie , Drosophila melanogaster/immunologie , Drosophila melanogaster/métabolisme , Système de signalisation des MAP kinases , Aedes/immunologie , Aedes/métabolisme , Aedes/virologie , Phénomènes physiologiques nutritionnels chez l'animal , Animaux , Arbovirus/pathogénicité , Système digestif/immunologie , Système digestif/métabolisme , Système digestif/virologie , Drosophila melanogaster/virologie , Femelle , Interactions hôte-pathogène/immunologie , Immunité innée , Vecteurs insectes/immunologie , Vecteurs insectes/métabolisme , Vecteurs insectes/virologie , Insuline/pharmacologie , Système de signalisation des MAP kinases/génétique , Système de signalisation des MAP kinases/immunologie , Interférence par ARNRÉSUMÉ
Bunyaviruses are an emerging group of medically important viruses, many of which are transmitted from insects to mammals. To identify host factors that impact infection, we performed a genome-wide RNAi screen in Drosophila and identified 131 genes that impacted infection of the mosquito-transmitted bunyavirus Rift Valley fever virus (RVFV). Dcp2, the catalytic component of the mRNA decapping machinery, and two decapping activators, DDX6 and LSM7, were antiviral against disparate bunyaviruses in both insect cells and adult flies. Bunyaviruses 5' cap their mRNAs by "cap-snatching" the 5' ends of poorly defined host mRNAs. We found that RVFV cap-snatches the 5' ends of Dcp2 targeted mRNAs, including cell cycle-related genes. Loss of Dcp2 allows increased viral transcription without impacting viral mRNA stability, while ectopic expression of Dcp2 impedes viral transcription. Furthermore, arresting cells in late S/early G2 led to increased Dcp2 mRNA targets and increased RVFV replication. Therefore, RVFV competes for the Dcp2-accessible mRNA pool, which is dynamically regulated and can present a bottleneck for viral replication.
Sujet(s)
Drosophila melanogaster/génétique , Drosophila melanogaster/virologie , Génome d'insecte/génétique , Orthobunyavirus/physiologie , Coiffes des ARN/métabolisme , Facteurs de transcription , Réplication virale/physiologie , Aedes/virologie , Animaux , Points de contrôle du cycle cellulaire , Lignée cellulaire , Protéines de Drosophila/génétique , Protéines de Drosophila/métabolisme , Drosophila melanogaster/métabolisme , Régulation de l'expression des gènes , Coiffes des ARN/génétique , Interférence par ARN , ARN messager/génétique , ARN messager/métabolisme , Facteurs de transcription/génétique , Facteurs de transcription/métabolismeRÉSUMÉ
Innate immune responses are characterized by precise gene expression whereby gene subsets are temporally induced to limit infection, although the mechanisms involved are incompletely understood. We show that antiviral immunity in Drosophila requires the transcriptional pausing pathway, including negative elongation factor (NELF) that pauses RNA polymerase II (Pol II) and positive elongation factor b (P-TEFb), which releases paused Pol II to produce full-length transcripts. We identify a set of genes that is rapidly transcribed upon arbovirus infection, including components of antiviral pathways (RNA silencing, autophagy, JAK/STAT, Toll, and Imd) and various Toll receptors. Many of these genes require P-TEFb for expression and exhibit pausing-associated chromatin features. Furthermore, transcriptional pausing is critical for antiviral immunity in insects because NELF and P-TEFb are required to restrict viral replication in adult flies and vector mosquito cells. Thus, transcriptional pausing primes virally induced genes to facilitate rapid gene induction and robust antiviral responses.
Sujet(s)
Arbovirus/pathogénicité , Drosophila/virologie , Immunité innée , ARN viral/métabolisme , Transcription génétique , Animaux , Arbovirus/immunologie , Drosophila/immunologie , Analyse de profil d'expression de gènesRÉSUMÉ
To understand the functional similarities of fly and mammalian taste receptors, we used a top-down approach that first established the fly sweetener-response profile. We employed the fruit fly Drosophila melanogaster, an omnivorous human commensal, and determined its sensitivity to an extended set of stimuli that humans find sweet. Flies were tested with all sweeteners in 2 assays that measured their taste reactivity (proboscis extension assay) and their ingestive preferences (free roaming ingestion choice test). A total of 21 sweeteners, comprised of 11 high-potency sweeteners, 2 amino acids, 5 sugars, 2 sugar alcohols, and a sweet salt (PbCl2), were tested in both assays. We found that wild-type Drosophila responded appetitively to most high-potency sweeteners preferred by humans, even those not considered sweet by rodents or new world monkeys. The similarities in taste preferences for sweeteners suggest that frugivorous/omnivorous apes and flies have evolved promiscuous carbohydrate taste detectors with similar affinities for myriad high-potency sweeteners. Whether these perceptual parallels are the result of convergent evolution of saccharide receptor-binding mechanisms remains to be determined.
Sujet(s)
Drosophila melanogaster/physiologie , Préférences alimentaires/physiologie , Édulcorants/composition chimique , Goût/physiologie , Acides aminés/composition chimique , Animaux , Évolution biologique , Glucides/composition chimique , Comportement alimentaire/physiologie , Femelle , Humains , Plomb/composition chimique , Mâle , Réflexe/physiologie , Polyols/composition chimiqueRÉSUMÉ
Two central issues in polyglutamine-induced neurodegeneration are the influence of the normal function of the disease protein and modulation by protein quality control pathways. By using Drosophila, we now directly link host protein function and disease pathogenesis to ubiquitin pathways in the polyglutamine disease spinocerebellar ataxia type 3 (SCA3). Normal human ataxin-3--a polyubiquitin binding protein with ubiquitin protease activity--is a striking suppressor of polyglutamine neurodegeneration in vivo. This suppressor activity requires ubiquitin-associated activities of the protein and is dependent upon proteasome function. Our results highlight the critical importance of host protein function in SCA3 disease and a potential therapeutic role of ataxin-3 activity for polyglutamine disorders.
Sujet(s)
Dégénérescence nerveuse/génétique , Dégénérescence nerveuse/prévention et contrôle , Protéines de tissu nerveux/génétique , Peptides/toxicité , Ubiquitine/métabolisme , Animaux , Animal génétiquement modifié , Ataxine-3 , Drosophila melanogaster/génétique , Humains , Maladie de Machado-Joseph/génétique , Mutagenèse dirigée , Protéines de tissu nerveux/métabolisme , Protéines nucléaires , Polymorphisme génétique , Protéines de répressionRÉSUMÉ
We tested whether proteins implicated in Huntington's and other polyglutamine (polyQ) expansion diseases can cause axonal transport defects. Reduction of Drosophila huntingtin and expression of proteins containing pathogenic polyQ repeats disrupt axonal transport. Pathogenic polyQ proteins accumulate in axonal and nuclear inclusions, titrate soluble motor proteins, and cause neuronal apoptosis and organismal death. Expression of a cytoplasmic polyQ repeat protein causes adult retinal degeneration, axonal blockages in larval neurons, and larval lethality, but not neuronal apoptosis or nuclear inclusions. A nuclear polyQ repeat protein induces neuronal apoptosis and larval lethality but no axonal blockages. We suggest that pathogenic polyQ proteins cause neuronal dysfunction and organismal death by two non-mutually exclusive mechanisms. One mechanism requires nuclear accumulation and induces apoptosis; the other interferes with axonal transport. Thus, disruption of axonal transport by pathogenic polyQ proteins could contribute to early neuropathology in Huntington's and other polyQ expansion diseases.