Sujet(s)
Pontage aortocoronarien , Tomodensitométrie , Adulte , Aortographie , Coronarographie , Femelle , Études de suivi , Humains , Mâle , Adulte d'âge moyenRÉSUMÉ
From karyometrical measurements performed by us and from investigations of other authors it results that the obtained values of nuclear volumes are smaller than real ones. This is caused by different sectioning planes of measured nuclei. Previously published corrections seem not to reflect the real error as they are based on the analysis of the circle, and not of the sphere. In the presented paper a method is given, which may be applicated to interpretation of the results of morphometrical measurements. The proposed method is based on the well known in statistical analysis 3o rule. The obtained results were applicated to a practical example.
Sujet(s)
Caryométrie/méthodes , Animaux , Microtomie , Rats , Statistiques comme sujetRÉSUMÉ
The aim of the work was the analysis of distribution of karyometric measurements for semi- and ultrathin sections. The investigation was performed using spheres (diameter 16.2 mm), which were randomly cut using a microtome knife. The diameters of sections were measured with ocular with accuracy 0.1 mm. It has been shown, that the mean value of the measurements does not correspond to the real value of the spheres' diameter, and it is distinctly lower. The theoretical analysis explaining this fenomenon was performed. The obtained results allow for estimation of real diameter of spherical nuclei of the some size.