RÉSUMÉ
Objective To evaluate the feasibility, safety and efficacy of intra-arterial thrombolytic therapy on elderly patients (≥ 80 years old) with acute ischemic stroke. Methods The clinical data of 86 patients with acute ischemic stroke, received intra-arterial thrombolytic therapy, were retrospectively analyzed; according to age differences, these patients were divided into advanced age group (≥80 years old, n=21) and common age group (<80 years old, n=65); and control group (≥80 years old, not receiving thrombolytic therapy, n=50) was established. The recanalization rate and early clinical improvement rate, and the incidence, recover rate and death rate of symptomatic intracerebral hemorrhage were evaluated in these patients after treatment. Results No significant differences in the favorite recanalization rate and short-term outcome, and the incidence of symptom intracranial hemorrhage were noted between the advanced age group and common age group (P=0.528, P=0.102,P=0.353). The incidence of symptom intracranial hemorrhage in the advanced age group was obviously higher than that in the control group (P=0.034); the recover rate of symptom ntracranial hemorrhage in the advanced age group (42.9%) was obviously lower than that in the common age group (50.8%), but significantly higher than that in the control group (16%, P=0.042, P=0.017). The mortality of the advanced age group was similar to that of the control group (23.8% versus 28%, P=0.816), but higher than that of common age group (23.8% versus 10.8%, P=0.034). Conclusion Relatively high feasibility, safety and efficacy of intra-arterial thrombolytic therapy are noted in elderly patients (≥80 years old) with acute ischemic stroke, demonstrating that the use of intra-arterial thrombolytic therapy in very elderly patients should not be avoided but pursued advisably.
RÉSUMÉ
Objective To investigate the inhibitory effect of minocycline (Mino) on lipopolysaccharide (LPS)-induced activation ofmicroglias and its srbsequent protective effects on nigral dopaminergic neurons. Methods A total of 25 male Sprague-Dawley (SD) rats were randomly allocated into three groups, sole LPS intervention group (LPS group)(n=10), LPS+Mino intervention group (n=10)(LPS+Mino group) and 5 rats injected with normal saline as the control group. Behavioral changes of all the rats were observed at 7 and 14 d, and the immunohistochemistry, in situ hybridization and Western blot were applied to observe the activation of microglias and the expression levels of positive neurons, mRNA and protein of tyrosine hydroxylase (TH) and OX-42. Results The rotation time of the LPS+Mino group was significantly lower than that of the LPS group at 7 and 14 d; the majority of microglias were activated in both the LPS and LPS+Mino groups, and the number of "amoeboid" microglias in the latter group was obviously lower than that in the former group, a few "ramified" microglias still existing in the latter group; Western blot assay showed that the protein levels of OX-42 expressed in the operative side midbrain of the two groups after induction were significantly higher than that in the control group, and the increment of (0.91±0.04) in the LPS+Mino group was significantly lower than that of (1.03±0.03) in the LPS group (P＜0.01); the number of TH positive neurons and the levels of TH mRNA and protein in the operative side midbrain of both groups after LPS treatment were obviously lower than in the control group, and the decreases of (21.54 ±4.89,39.87 ±7.03and 0.42±0.03) in the LPS group were significantly higher than that of (53.41±8.36,65.12±9.06 and 0.63±0.04) in the LPS+Mino group (P＜0.01). Conclusion Mino may protect nigral dopaminergic neurons against lipopolysaccharide-induced neurotoxicity by inhibiting microglia activation and subsequently prevent the progression of LPS-induced Parkinson's disease (PD).[ Key words ] Minocycline; Lipopolysaccharide; Dopaminergic neurons; Microglia;Parkinson's disease
RÉSUMÉ
Objective To further investigated the effect of minocycline on the inhibition of microglial activation and subsequent protection of nigral DA neuron.Methods 20 rats injected with LPS in the substantia nigra (SN) were randomly divided into two groups (LPS group and LPS+Minocycline group).The behavior was observed on the 7~(th) d and 14~(th) d.The immunohistoehemistry,in situ hybridization and Western-blot were used to detect the levels of positive neuron,mRNA,protein of TH and OX-42. Results The slightly rotational behavior was observed in LPS+Minoeyeline group.The majority of mieroglias were activated in the two groups.Some microglia in the SNpc remained ramified in LPS+ Minocycline group.The numbers of hypertophie microglia in LPS+Minoeyeline group were less than that in LPS group.Western-blot showed that the protein of OX-42 in two LPS groups was higher than in normal group(P