[Effects of additives in blood collection tubes on testing the alcohol concentration in blood samples].

OBJECTIVE: To discuss blood collection tubes with different additives and their effects on the testing results of alcohol concentration in blood samples. METHODS: Blood samples from 10 volunteers were collected 2 hours after drinking with seven different types of disposable vacuum blood collection tubes, including ordinary tube without anticoagulant, coagulant tube, separating gel-coagulant tube, sodium citrate (1:4) tube, sodium citrate (1:9) tube, sodium citrate (9:1) tube and EDTA-K2 tube. The alcohol concentrations in these blood samples were analyzed by headspace gas chromatography. RESULTS: The concentration testing results of the same blood samples in different types of tubes were different from one to another. The sequence was as follows: separating gel-coagulant tube > coagulant tube > ordi- nary tube without anticoagulant > EDTA-K2 tube> sodium citrate (1:9) tube> sodium citrate (1:4) tube, whereas the results of the same blood sample in sodium citrate (1:9) tube and sodium citrate (9:1) tube showed no obvious difference. CONCLUSION: It is better to collect a suspicious drunk driver's blood sam- ple using a disposable vacuum blood collection tube, with the EDTA-K2 tube being preferred.

[A study in extraction method of buprenorphine in urine].

OBJECTIVE: The extraction method for separating buprenorphine from urine was described. METHODS: Buprenorphine was extracted with chloroform at pH7 or with SPE (extracted by organic support 401 at pH10.8, then eluted with chloroform), finally determined by GC-NPD. RESULTS: The extracted yields of the analyte in specimens were 86.6% by solution-phase extraction and 83.0% by solid-phase extraction (SPE) respectively. CONCLUSION: These two methods are simple and accurate for separating buprenorphine from urine.