RÉSUMÉ
In the realm of skin injury management, the expedited closure of wounds, prevention of scar formation, and enhancement of the healing process are of critical significance. The creation of economical dressings that effectively facilitate swift wound sealing in the initial phase of skin trauma while curbing scar development represents a promising avenue for clinical utility. Within the context of this investigation, we synthesized a novel hydrogel composed of chitosan (CS), carboxylated poly(vinyl alcohol) (PVA-COOH) via a Schiff base reaction between carboxylated PVA and chitosan, yielding networks abundant in amide bonds. Following this, a chitosan/carboxylated PVA/poly(N-isopropylacrylamide) hydrogel (CNP) was engineered by incorporating poly-N-isopropylacrylamide chains for interpenetration at ambient temperature. Our findings indicate that the CNP hydrogel exhibits favorable degradability and swelling characteristics. Moreover, it possesses favorable antimicrobial efficacy and biocompatibility. In a murine full-thickness skin injury model, the hydrogel was found to expedite wound healing by augmenting granulation tissue formation, mitigating wound inflammation, and promoting angiogenesis.
RÉSUMÉ
Current limitations in mechanical performance and foreign body reactions (FBR) often lead to implant failure, restricting the application of bioceramic scaffolds. This study presents a novel 3D-printed scaffold that combines the release of anti-inflammatory drugs with osteogenic stimulation. Initially, the inorganic and organic phases were integrated to ensure the scaffold's mechanical integrity through catechol chemistry and the electrostatic interactions between tannic acid and quaternary ammonium chitosan. Subsequently, layers of polydopamine-encapsulated puerarin-loaded zeolitic imidazolate framework-8 (ZIF-8) were self-assembled onto the stent's surface, creating the drug-loaded scaffold that improved drug release without altering the scaffold's structure. Compared with unloaded scaffolds, the puerarin-loaded scaffold demonstrated excellent osteogenic differentiation properties along with superior anti-inflammatory and osteogenic effects in a range of in vitro and in vivo studies. RNA sequencing clarified the role of the TNF and NF/κB signaling pathways in these effects, further supporting the scaffold's osteogenic potential. This study introduces a novel approach for creating drug-loaded scaffolds, providing a unique method for treating cancellous bone defects.