HER2-CD3-Fc Bispecific Antibody-Encoding mRNA Delivered by Lipid Nanoparticles Suppresses HER2-Positive Tumor Growth.

The human epidermal growth factor receptor 2 (HER2) is a transmembrane tyrosine kinase receptor and tumor-associated antigen abnormally expressed in various types of cancer, including breast, ovarian, and gastric cancer. HER2 overexpression is highly correlated with increased tumor aggressiveness, poorer prognosis, and shorter overall survival. Consequently, multiple HER2-targeted therapies have been developed and approved; however, only a subset of patients benefit from these treatments, and relapses are common. More potent and durable HER2-targeted therapies are desperately needed for patients with HER2-positive cancers. In this study, we developed a lipid nanoparticle (LNP)-based therapy formulated with mRNA encoding a novel HER2-CD3-Fc bispecific antibody (bsAb) for HER2-positive cancers. The LNPs efficiently transfected various types of cells, such as HEK293S, SKOV-3, and A1847, leading to robust and sustained secretion of the HER2-CD3-Fc bsAb with high binding affinity to both HER2 and CD3. The bsAb induced potent T-cell-directed cytotoxicity, along with secretion of IFN-λ, TNF-α, and granzyme B, against various types of HER2-positive tumor cells in vitro, including A549, NCI-H460, SKOV-3, A1847, SKBR3, and MDA-MB-231. The bsAb-mediated antitumor effect is highly specific and strictly dependent on its binding to HER2, as evidenced by the gained resistance of A549 and A1847 her2 knockout cells and the acquired sensitivity of mouse 4T1 cells overexpressing the human HER2 extracellular domain (ECD) or epitope-containing subdomain IV to the bsAb-induced T cell cytotoxicity. The bsAb also relies on its binding to CD3 for T-cell recruitment, as ablation of CD3 binding abolished the bsAb's ability to elicit antitumor activity. Importantly, intratumoral injection of the HER2-CD3-Fc mRNA-LNPs triggers a strong antitumor response and completely blocks HER2-positive tumor growth in a mouse xenograft model of human ovarian cancer. These results indicate that the novel HER2-CD3-Fc mRNA-LNP-based therapy has the potential to effectively treat HER2-positive cancer.

Interactive Table Synthesis with Natural Language.

Tables are a ubiquitous data format for insight communication. However, transforming data into consumable tabular views remains a challenging and time-consuming task. To lower the barrier of such a task, research efforts have been devoted to developing interactive approaches for data transformation, but many approaches still presume that their users have considerable knowledge of various data transformation concepts and functions. In this study, we leverage natural language (NL) as the primary interaction modality to improve the accessibility of average users to performing complex data transformation and facilitate intuitive table generation and editing. Designing an NL-driven data transformation approach introduces two challenges: a) NL-driven synthesis of interpretable pipelines and b) incremental refinement of synthesized tables. To address these challenges, we present NL2Rigel, an interactive tool that assists users in synthesizing and improving tables from semi-structured text with NL instructions. Based on a large language model and prompting techniques, NL2Rigel can interpret the given NL instructions into a table synthesis pipeline corresponding to Rigel specifications, a declarative language for tabular data transformation. An intuitive interface is designed to visualize the synthesis pipeline and the generated tables, helping users understand the transformation process and refine the results efficiently with targeted NL instructions. The comprehensiveness of NL2Rigel is demonstrated with an example gallery, and we further confirmed NL2Rigel's usability with a comparative user study by showing that the task completion time with NL2Rigel is significantly shorter than that with the original version of Rigel with comparable completion rates.

CAR-NK Cells Generated with mRNA-LNPs Kill Tumor Target Cells In Vitro and In Vivo.

Natural killer (NK) cells are cytotoxic lymphocytes that are critical for the innate immune system. Engineering NK cells with chimeric antigen receptors (CARs) allows CAR-NK cells to target tumor antigens more effectively. In this report, we present novel CAR mRNA-LNP (lipid nanoparticle) technology to effectively transfect NK cells expanded from primary PBMCs and to generate functional CAR-NK cells. CD19-CAR mRNA and BCMA-CAR mRNA were embedded into LNPs that resulted in 78% and 95% CAR expression in NK cells, respectively. BCMA-CAR-NK cells after transfection with CAR mRNA-LNPs killed multiple myeloma RPMI8226 and MM1S cells and secreted IFN-gamma and Granzyme B in a dose-dependent manner in vitro. In addition, CD19-CAR-NK cells generated with CAR mRNA-LNPs killed Daudi and Nalm-6 cells and secreted IFN-gamma and Granzyme B in a dose-dependent manner. Both BCMA-CAR-NK and CD19-CAR-NK cells showed significantly higher cytotoxicity, IFN-gamma, and Granzyme B secretion compared with normal NK cells. Moreover, CD19-CAR-NK cells significantly blocked Nalm-6 tumor growth in vivo. Thus, non-viral delivery of CAR mRNA-LNPs can be used to generate functional CAR-NK cells with high anti-tumor activity.

Preparation of a novel Bi9O7.5S6/SnS composite film with improved photoelectric properties.

Atomically thin two-dimensional (2D) bismuth oxychalcogenides have been considered as promising candidates for high-speed and low-power photoelectronic devices due to their high charge carrier mobility and excellent environmental stability. However, the photoelectric performance of their bulk materials still falls short of expectations. Herein, a novel Bi9O7.5S6/SnS composite film with a type-II heterojunction was successfully prepared by combining hydrothermal and knife-coating techniques. The crystal structure, morphology, and optical properties were systematically investigated. Under 1 V bias voltage, the photocurrent of the Bi9O7.5S6/SnS composite film can be obtained as 107 µA cm-2, which is about 29.9 times and 93.9 times higher than that of bare Bi9O7.5S6 and SnS, respectively. The type-II heterojunction has played a significant role in improving the photoelectric performance of the Bi9O7.5S6/SnS composite film by facilitating the separation and transfer of photo-generated carriers. This work sheds light on the design and development of new bismuth-based composite materials for advanced photoelectric and photocatalytic applications.

mRNA-Lipid Nanoparticle (LNP) Delivery of Humanized EpCAM-CD3 Bispecific Antibody Significantly Blocks Colorectal Cancer Tumor Growth.

The epithelial cell adhesion molecule (EpCAM) is often overexpressed in many types of tumors, including colorectal cancer. We sequenced and humanized an EpCAM mouse antibody and used it to develop bispecific EpCAM-CD3 antibodies. Three different designs were used to generate bispecific antibodies such as EpCAM-CD3 CrossMab knob-in-hole, EpCAM ScFv-CD3 ScFv (BITE), and EpCAM ScFv-CD3 ScFv-human Fc designs. These antibody designs showed strong and specific binding to the EpCAM-positive Lovo cell line and T cells, specifically killed EpCAM-positive Lovo cells and not EpCAM-negative Colo741 cells in the presence of T cells, and increased T cells' IFN-gamma secretion in a dose-dependent manner. In addition, transfection of HEK-293 cells with EpCAM ScFv-CD3 ScFv human Fc mRNA-LNPs resulted in antibody secretion that killed Lovo cells and did not kill EpCAM-negative Colo741 cells. The antibody increased IFN-gamma secretion against Lovo target cells and did not increase it against Colo741 target cells. EpCAM-CD3 hFc mRNA-LNP transfection of several cancer cell lines (A1847, C30, OVCAR-5) also demonstrated functional bispecific antibody secretion. In addition, intratumoral delivery of the EpCAM-CD3 human Fc mRNA-LNPs into OVCAR-5 tumor xenografts combined with intravenous injection of T cells significantly blocked xenograft tumor growth. Thus, EpCAM-CD3 hFc mRNA-LNP delivery to tumor cells shows strong potential for future clinical studies.

The prevalence and risk factors of different degrees of stress urinary incontinence in Chinese women: A community-based cross-sectional study.

AIM: To investigate the prevalence of SUI and explore the factors that could influence the severity of SUI in adult females. DESIGN: A cross-sectional study. METHODS: A total of 1178 subjects were assessed using a risk-factor questionnaires and International Consultation on Incontinence Questionnaire Short Form (ICIQ-SF) and then divided into no SUI group, mild SUI group and moderate-to-severe SUI group according to the ICIQ-SF score. Univariate analysis between adjacent groups and ordered logistic regression models in three groups were then performed to analysis the possible associated factor with the progressive of SUI. RESULTS: The prevalence of SUI among adult women was 22.2% of them; 16.2% and 6% had mild SUI and moderate-to-severe SUI, respectively. Moreover, logistic analysis revealed that age, BMI, smoking, position preference for urination, urinary tract infections, urinary leaks during pregnancy, gynaecological inflammation and poor sleep quality were independent risk for the severity of SUI. CONCLUSION: SUI symptoms were mostly mild among Chinese females, specific risk factors such as unhealthy living habits and urination behaviours increased the risk of SUI and the aggravation of symptoms. Therefore, targeted interventions should be formulated for women to delay disease progression.

Non-mechanical self-alignment system for free-space optical communications based on a cascaded liquid crystal optical antenna.

Free-space optical (FSO) communication has attracted extensive attention in recent years. To maintain a reliable FSO link, two main issues need to be addressed: beam drift and vibration. In this paper, we demonstrate a non-mechanical self-alignment system based on a cascaded liquid crystal optical antenna, in which a frequency decoupled hybrid integration Kalman filter (FDHI-KF) method is proposed to achieve predictive beam drift tracking and vibration mitigation. By leveraging the integrated control on our lab-made liquid crystal phase modulation devices, and implementing the adaptive algorithm on a heterogeneous field programmable gate array (FPGA), this system is capable of realizing precise self-alignment without any moving parts. Experiments are conducted to verify its performance in practical applications. We envision it to set a benchmark for future liquid crystal non-mechanical beam-steering systems in FSO communications.

Rigel: Transforming Tabular Data by Declarative Mapping.

We present Rigel, an interactive system for rapid transformation of tabular data. Rigel implements a new declarative mapping approach that formulates the data transformation procedure as direct mappings from data to the row, column, and cell channels of the target table. To construct such mappings, Rigel allows users to directly drag data attributes from input data to these three channels and indirectly drag or type data values in a spreadsheet, and possible mappings that do not contradict these interactions are recommended to achieve efficient and straightforward data transformation. The recommended mappings are generated by enumerating and composing data variables based on the row, column, and cell channels, thereby revealing the possibility of alternative tabular forms and facilitating open-ended exploration in many data transformation scenarios, such as designing tables for presentation. In contrast to existing systems that transform data by composing operations (like transposing and pivoting), Rigel requires less prior knowledge on these operations, and constructing tables from the channels is more efficient and results in less ambiguity than generating operation sequences as done by the traditional by-example approaches. User study results demonstrated that Rigel is significantly less demanding in terms of time and interactions and suits more scenarios compared to the state-of-the-art by-example approach. A gallery of diverse transformation cases is also presented to show the potential of Rigel's expressiveness.

Atomic Horizons Interpretation on Enhancing Electrochemical Performance of Ni-Rich NCM Cathode via W Doping: Dual Improvements in Electronic and Ionic Conductivities from DFT Calculations and Experimental Confirmation.

The rapid capacity degradation and poor rate capability hinder the application of Rich-Ni layered LiNix Coy Mnz O2 (NCM) as cathode materials for high-energy lithium-ion batteries. In this study, density functional theory (DFT) calculations, combined with conventional electrochemical measurements, reveal from the atomic view that the dual improvements in electronic and ionic conductivities are the main facts for the property enhancement. The bandgap of the cathode material is reduced to 1.1623 eV due to the increased number of electrons near the Fermi level after W intercalation. Such improved electronic conductivity subsequently leads to a suppressed polarization and reduced resistance, enabling an improved cycle life of up to 93.97% after 100 cycles at 0.5 C. Furthermore, the doping with W6+ also introduced a strong WO bond into the layered structure so that the thickness of the Li slab is expanded to 2.6476 Å, which reduces the energy barrier from 0.355 to 0.308 eV for the migration of Li+ within the Li slab, as confirmed by the DFT calculation. Consequently, the rate performance is greatly improved due to the reduced diffusion energy, with a specific capacity of 159.11 mAg-1 even at 5 C rate, indicating high potential for future applications.

Fabrication and enhanced photoelectric properties of a novel Bi9O7.5S6/CdS composite film.

Layered bismuth oxychalcogenides have been demonstrated as potential candidates for high-speed and low-power electronics due to their outstanding environmental stability and high carrier mobility, but the photoelectric performance of bulk species is still far from satisfactory. Herein, a novel Bi9O7.5S6/CdS composite film with a type-II heterojunction has been successfully prepared by combining chemical bath deposition (CBD) and spin-coating technologies. The structure, morphology, optical and photoelectric properties of the samples were investigated systematically. The photoelectric current of the Bi9O7.5S6/CdS composite film was obtained as 32.49 µA cm-2 at 1 V, which is about 13.9-fold and 3.3-fold higher than those of bare Bi9O7.5S6 and CdS. An enhanced photoelectric response and photostability were achieved in the Bi9O7.5S6/CdS composite film, and can be appropriately attributed to the improved separation and transfer of photogenerated carriers driven by the type-II heterojunction. This work offers a promising route to develop high-performance visible-light photoelectric devices with type-II heterojunctions.

CHMM Object Detection Based on Polygon Contour Features by PSM.

Since the conventional split-merge algorithm is sensitive to the object scale variance and splitting starting point, a piecewise split-merge polygon-approximation method is proposed to extract the object contour features. Specifically, the contour corner is used as the starting point for the contour piecewise approximation to reduce the sensitivity of the contour segment for the starting point; then, the split-merge algorithm is used to implement the polygon approximation for each contour segment. Both the distance ratio and the arc length ratio instead of the distance error are used as the iterative stop condition to improve the robustness to the object scale variance. Both the angle and length as two features describe the shape of the contour polygon; they have a strong coupling relationship since they affect each other along the contour order relationship. To improve the description correction of the contour, these two features are combined to construct a Coupled Hidden Markov Model to detect the object by calculating the probability of the contour feature. The proposed algorithm is validated on ETHZ Shape Classes and INRIA Horses standard datasets. Compared with other contour-based object-detection algorithms, the proposed algorithm reduces the feature number and improves the object-detection rate.

Universal dimension reduced phase compensation algorithm for an optical phased array.

Optical phased arrays (OPAs) can achieve non-mechanical beam deflection. Many types of OPA face the problem of low deflection efficiency due to the phase distortion induced by mutual coupling between nearby channels. In this Letter, a universal optimization algorithm is proposed to compensate for this structural phase distortion, in which the adjacent sampling principal component analysis (AS-PCA) method is introduced to reduce the dimension of the solution space. Simulations and experimental results on different classes of OPA verified that this method can considerably optimize the deflection beam with a rapid convergence speed, irrespective of the scale of OPA, and maintain the universal feature, laying the foundation for large-scale, high-density OPA in-line optimization. We envision it to become a general method on different platforms.

Effectiveness of internet-based support interventions on patients with breast cancer: a systematic review and narrative synthesis.

OBJECTIVE: To identify the elements of internet-based support interventions and assess their effectiveness at reducing psychological distress, anxiety and/or depression, physical variables (prevalence, severity and distress from physical symptoms) and improving quality of life, social support and self-efficacy among patients with breast cancer. DESIGN: Systematic review and narrative synthesis. DATA SOURCES: Web of Science, Cochrane Library, PubMed, MEDLINE, PsycINFO, CINAHL, CNKI, Wanfang and VIP from over the past 5 years of each database to June 2021. ELIGIBILITY CRITERIA FOR STUDY SELECTION: Included were randomised controlled trials (RCTs) or quasi-experimental (QE) studies focusing on internet-based support interventions in patients with breast cancer. DATA EXTRACTION AND SYNTHESIS: Reviewers independently screened, extracted data and assessed risk of bias (Cochrane Collaboration' risk of bias tool, Joanna Briggs Institute reviewer's manual). Narrative synthesis included the effect and elements of internet-based support interventions for women with breast cancer. RESULTS: Out of 2842 articles, 136 qualified articles were preliminarily identified. After further reading the full text, 35 references were included, including 30 RCTs and five QE studies. Internet-based support interventions have demonstrated positive effects on women's quality of life and physical variables, but inconsistent effectiveness has been found on psychological distress, symptoms of anxiety and/or depression, social support and self-efficacy. CONCLUSIONS: Internet-based support interventions are increasingly being used as clinically promising interventions to promote the health outcomes of patients with breast cancer. Future research needs to implement more rigorous experimental design and include sufficient sample size to clarify the effectiveness of this internet-based intervention. PROSPERO REGISTRATION NUMBER: CRD42021271380.

Bispecific BCMA-CD3 Antibodies Block Multiple Myeloma Tumor Growth.

BCMA antigen is overexpressed in multiple myeloma cells and has been shown to be a promising target for novel cellular and antibody therapeutics. The humanized BCMA (clone 4C8A) antibody that effectively targeted multiple myeloma in a CAR (chimeric antigen receptor) format was used for designing several formats of bispecific BCMA-CD3 antibodies. Several different designs of univalent and bivalent humanized BCMA-CD3 CrossMAB and BCMA-FAB-CD3 ScFv-Fc antibodies were tested for binding with BCMA-positive cells and T cells and for killing by real time cytotoxic activity and IFN-gamma secretion with CHO-BCMA target cells and with multiple myeloma MM1S and H929 cell lines. All BCMA-CD3 antibodies demonstrated specific binding by FACS to CHO-BCMA, multiple myeloma cells, and to T cells with affinity Kd in the nM range. All antibodies with T cells specifically killed CHO-BCMA and multiple myeloma cells in a dose-dependent manner. The BCMA-CD3 antibodies with T cells secreted IFN-gamma with EC50 in the nM range. In addition, three BCMA bispecific antibodies had high in vivo efficacy using an MM1S xenograft NSG mouse model. The data demonstrate the high efficacy of novel hBCMA-CD3 antibodies with multiple myeloma cells and provide a basis for future pre-clinical and clinical development.

Fast, closed-loop iterative system-on-chip of deflection efficiency enhancement for a liquid crystal optical phased array.

To implement a liquid crystal optical phased array (LC-OPA) on a practical free-space laser communication terminal, there are two essential parameters: insertion loss and the closed-loop bandwidth required to meet the dynamic linking condition of the acquisition-tracking-pointing sub-system. Real-time hardware platforms and deflection efficiency optimization algorithms have been suggested since the invention of LC-OPA. In this paper, the so-called ZYNQ platform, a field-programmable-gate-array-based heterogeneous system-on-chip (SoC), is utilized to keep real-time response and accelerate data generation, such as beam steering, beamforming, beam enhancement, etc. In addition, a novel, to the best of our knowledge, optimization algorithm is proposed on the concept of dimension reduction of the number of objective variables. After deploying on this heterogeneous SoC platform, numerical simulations and experimental results both verify that, compared to the conventional PC-based system, the integrated SoC platform offers 15.8 times faster iterative speed, a rapid convergence rate, and excellent robustness, yet with less usage of power, physical size, and monetary cost. The efficiency enhancement process costs only a few seconds at any angle, laying the foundation for practical in-line applications.

A Design of FPGA-Based Neural Network PID Controller for Motion Control System.

In the actual industrial production process, the method of adaptively tuning proportional-integral-derivative (PID) parameters online by neural network can adapt to different characteristics of different controlled objects better than the controller with PID. However, the commonly used microcontroller unit (MCU) cannot meet the application scenarios of real time and high reliability. Therefore, in this paper, a closed-loop motion control system based on BP neural network (BPNN) PID controller by using a Xilinx field programmable gate array (FPGA) solution is proposed. In the design of the controller, it is divided into several sub-modules according to the modular design idea. The forward propagation module is used to complete the forward propagation operation from the input layer to the output layer. The PID module implements the mapping of PID arithmetic to register transfer level (RTL) and is responsible for completing the output of control amount. The main state machine module generates enable signals that control the sequential execution of each sub-module. The error backpropagation and weight update module completes the update of the weights of each layer of the network. The peripheral modules of the control system are divided into two main parts. The speed measurement module completes the acquisition of the output pulse signal of the encoder and the measurement of the motor speed. The pulse width modulation (PWM) signal generation module generates PWM waves with different duty cycles to control the rotation speed of the motor. A co-simulation of Modelsim and Simulink is used to simulate and verify the system, and a test analysis is also performed on the development platform. The results show that the proposed system can realize the self-tuning of PID control parameters, and also has the characteristics of reliable performance, high real-time performance, and strong anti-interference. Compared with MCU, the convergence speed is far more than three orders of magnitude, which proves its superiority.

Predicting plant diversity in beach wetland downstream of Xiaolangdi reservoir with UAV and satellite multispectral images.

Accurate and timely acquisition of plant diversity information downstream of the reservoir is helpful to understand the impact mechanism of reservoir operation on wetland plant diversity and formulate reasonable water and sediment regulation strategies. In this study, we conducted field surveys in two communities (Phragmites australis and Tamarix chinensis) at a typical wetland in the lower reaches of Xiaolangdi Reservoir on the Yellow River, and employed UAV and Gaofen 1B multispectral images to estimate the wetland plant diversity. Results showed that most diversity indexes had a higher correlation with the mean of spectral vegetation indexes (DVI, RVI, NDVI, SAVI, and MSAVI). The diversity indexes (C_SP and C_SW) constructed by relative coverage had a better overall correlation with spectral indexes. Interestingly, opposite correlations were found between Tamarix chinensis and Phragmites australis plots. We further gave a deep insight into the interspecific associations in Phragmites australis and Tamarix chinensis plots with the variance ratio (VR) method. It was found that plant species in Tamarix chinensis plot showed positive association (VR > 1), with a VR value of 1.095. Plant species in Phragmites australis plot had a negative association (VR < 1), with a VR value of 0.983. In Phragmites australis plot, C_SP and C_SW showed a significant decreasing trend (r2 of 0.36 and 0.33 respectively, and P values less than 0.001) with the increase of Phragmites australis coverage. Moreover, the effect of spatial resolution was not significant on plant diversity estimation. Correlations between remote sensing indexes and diversity indexes were improved with the quadrat size changing from 2 m × 2 m to 4 m × 4 m. These findings demonstrate promising approaches for remote sensing of wetland plant diversity and indicate that the type of wetland plant community determines the relationship between diversity index and spectral index.

Multiband Spectrum Sensing and Power Allocation for aCognitive Radio-Enabled Smart Grid.

As part of an Internet of Things (IoT) framework, the Smart Grid (SG) relies on advanced communication technologies for efficient energy management and utilization. Cognitive Radio (CR), which allows Secondary Users (SUs) to opportunistically access and use the spectrum bands owned by Primary Users (PUs), is regarded as the key technology of the next-generation wireless communication. With the assistance of CR technology, the quality of communication in the SG could be improved. In this paper, based on a hybrid CR-enabled SG communication network, a new system architecture for multiband-CR-enabled SG communication is proposed. Then, some optimization mathematical models are also proposed to jointly find the optimal sensing time and the optimal power allocation strategy. By using convex optimization techniques, several optimal methods are proposed to maximize the data rate of multiband-CR-enabled SG while considering the minimum detection probabilities to the active PUs. Finally, simulations are presented to show the validity of the proposed methods.

Novel CS1 CAR-T Cells and Bispecific CS1-BCMA CAR-T Cells Effectively Target Multiple Myeloma.

Multiple myeloma (MM) is a hematological cancer caused by abnormal proliferation of plasma cells in the bone marrow, and novel types of treatment are needed for this deadly disease. In this study, we aimed to develop novel CS1 CAR-T cells and bispecific CS1-BCMA CAR-T cells to specifically target multiple myeloma. We generated a new CS1 (CD319, SLAM-7) antibody, clone (7A8D5), which specifically recognized the CS1 antigen, and we applied it for the generation of CS1-CAR. CS1-CAR-T cells caused specific killing of CHO-CS1 target cells with secretion of IFN-gamma and targeted multiple myeloma cells. In addition, bispecific CS1-BCMA-41BB-CD3 CAR-T cells effectively killed CHO-CS1 and CHO-BCMA target cells, killed CS1/BCMA-positive multiple myeloma cells, and secreted IFN-gamma. Moreover, CS1-CAR-T cells and bispecific CS1-BCMA CAR-T cells effectively blocked MM1S multiple myeloma tumor growth in vivo. These data for the first time demonstrate that novel CS1 and bispecific CS1-BCMA-CAR-T cells are effective in targeting MM cells and provide a basis for future clinical trials.

Evolution of Structural and Electronic Properties of TiSe2 under High Pressure.

A pressure-induced structural phase transition and its intimate link with the superconducting transition was studied for the first time in TiSe2 up to 40 GPa at room temperature using X-ray diffraction, transport measurement, and first-principles calculations. We demonstrate the occurrence of a first-order structural phase transition at 4 GPa from the standard trigonal structure (S.G.P3̅m1) to another trigonal structure (S-G-P3̅c1). Additionally, at 16 GPa, the P3̅c1 phase spontaneously transforms into a monoclinic C2/m phase, and above 24 GPa, the C2/m phase returns to the initial P3̅m1 phase. Electrical transport results show that metallization occurs above 6 GPa. The charge density wave observed at ambient pressure is suppressed upon compression up to 2 GPa with the emergence of superconductivity at 2.5 GPa, with a critical temperature (Tc) of 2 K. A structural transition accompanies the emergence of superconductivity that persists up to 4 GPa. The results demonstrate that the pressure-induced phase transitions explored by the experiments along with the theoretical predictions may open the door to a new path for searching and controlling the phase diagrams of transition metal dichalcogenides.