RÉSUMÉ
ABSTRACT Objectives The aim of this study was to explore the effect of capsaicin on expression patterns of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) and trigeminal subnucleus caudalis (Vc) following experimental tooth movement. Material and Methods Male Sprague-Dawley rats were used in this study and divided into small-dose capsaicin+force group, large-dose capsaicin+force group, saline+force group, and no force group. Closed coil springs were used to mimic orthodontic forces in all groups except for the no force group, in which springs were inactivated. Capsaicin and saline were injected into periodontal tissues. Rats were euthanized at 0 h, 12 h, 1 d, 3 d, 5 d, and 7 d following experimental tooth movement. Then, TG and Vc were obtained for immunohistochemical staining and western blotting against CGRP. Results Immunohistochemical results indicated that CGRP positive neurons were located in the TG, and CGRP immunoreactive fibers were distributed in the Vc. Immunohistochemical semiquantitative analysis and western blotting analysis demonstrated that CGRP expression levels both in TG and Vc were elevated at 12 h, 1 d, 3 d, 5 d, and 7 d in the saline + force group. However, both small-dose and large-dose capsaicin could decrease CGRP expression in TG and Vc at 1 d and 3 d following experimental tooth movement, as compared with the saline + force group. Conclusions These results suggest that capsaicin could regulate CGRP expression in TG and Vc following experimental tooth movement in rats.
Sujet(s)
Animaux , Mâle , Mouvement dentaire/méthodes , Sous-noyau caudal du noyau spinal du nerf trijumeau/effets des médicaments et des substances chimiques , Capsaïcine/pharmacologie , Peptide relié au gène de la calcitonine/effets des médicaments et des substances chimiques , Ganglion trigéminal/effets des médicaments et des substances chimiques , Agents du système nerveux sensoriel/pharmacologie , Valeurs de référence , Facteurs temps , Sous-noyau caudal du noyau spinal du nerf trijumeau/composition chimique , Algie faciale , Immunohistochimie , Chlorure de sodium , Répartition aléatoire , Peptide relié au gène de la calcitonine/analyse , Technique de Western , Ganglion trigéminal/composition chimique , Reproductibilité des résultats , Rat Sprague-DawleyRÉSUMÉ
Objectives: The aim of this study was to explore the effect of capsaicin on expression patterns of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) and trigeminal subnucleus caudalis (Vc) following experimental tooth movement. Material and Methods: Male Sprague-Dawley rats were used in this study and divided into small-dose capsaicin+force group, large-dose capsaicin+force group, saline+force group, and no force group. Closed coil springs were used to mimic orthodontic forces in all groups except for the no force group, in which springs were inactivated. Capsaicin and saline were injected into periodontal tissues. Rats were euthanized at 0 h, 12 h, 1 d, 3 d, 5 d, and 7 d following experimental tooth movement. Then, TG and Vc were obtained for immunohistochemical staining and western blotting against CGRP. Results: Immunohistochemical results indicated that CGRP positive neurons were located in the TG, and CGRP immunoreactive fibers were distributed in the Vc. Immunohistochemical semiquantitative analysis and western blotting analysis demonstrated that CGRP expression levels both in TG and Vc were elevated at 12 h, 1 d, 3 d, 5 d, and 7 d in the saline + force group. However, both small-dose and large-dose capsaicin could decrease CGRP expression in TG and Vc at 1 d and 3 d following experimental tooth movement, as compared with the saline + force group. Conclusions: These results suggest that capsaicin could regulate CGRP expression in TG and Vc following experimental tooth movement in rats.
Sujet(s)
Peptide relié au gène de la calcitonine/effets des médicaments et des substances chimiques , Capsaïcine/pharmacologie , Agents du système nerveux sensoriel/pharmacologie , Mouvement dentaire/méthodes , Sous-noyau caudal du noyau spinal du nerf trijumeau/effets des médicaments et des substances chimiques , Ganglion trigéminal/effets des médicaments et des substances chimiques , Animaux , Technique de Western , Peptide relié au gène de la calcitonine/analyse , Algie faciale , Immunohistochimie , Mâle , Répartition aléatoire , Rat Sprague-Dawley , Valeurs de référence , Reproductibilité des résultats , Chlorure de sodium , Facteurs temps , Sous-noyau caudal du noyau spinal du nerf trijumeau/composition chimique , Ganglion trigéminal/composition chimiqueRÉSUMÉ
OBJECTIVE: To establish normal ranges of F-wave measures in China as compared to published data in Brazil and Japan. METHOD: We studied F waves in 127 healthy Chinese subjects, aged 21-78 years old, stimulating the median, ulnar, tibial and peroneal nerves distally and proximaly. RESULT: The F-wave latencies had a linear correlation to the subject height in all nerves tested, showing a steeper (P < 0.05) regression line in Brazil compared to China and Japan for the tibial nerve. The Chinese population also had a higher distally elicited F-wave persistence and faster F wave conduction velocities than previously reported for both ulnar and tibial nerves. These values showed significant difference (P < 0.05) compared to Brazil but not to Japan. CONCLUSION: F-wave latencies show a linear correlation to subject height, although the slope of regression lines varies among countries, reflecting the difference in F-wave persistence and F-wave conduction velocity. SIGNIFICANCE: Our study provides a rational for the use of a latency-height nomogram in clinical studies and underscores the importance of developing country specific normative data.