[Corrigendum] Bax inhibitor­1 suppresses early brain injury following experimental subarachnoid hemorrhage in rats.

Following the publication of the above article, an interested reader drew to the authors' attention that, in Fig. 6 on p. 2898, the 'SAH' and 'SAH+NC' data panels contained an apparently overlapping section of data, such that these data appeared to have been derived from the same original source, even though they were intended to show the results from differently performed experiments. The authors have examined their original data, and realize that the 'SAH+NC' data panel had inadvertently been selected incorrectly for this figure. In addition, in response to a further query from the reader, the authors wished to point out that the standard deviations in their study were statistically analysed using GraphPad Prism software version 5.0a. The revised version of Fig. 6, now showing the correct data for the 'SAH+NC' experiment, is shown on the next page. The authors can confirm that the errors associated with this figure did not have any significant impact on either the results or the conclusions reported in this study, and all the authors agree with the publication of this Corrigendum. The authors are grateful to the Editor of International Journal of Molecular Medicine for allowing them the opportunity to publish this Corrigendum; furthermore, they apologize to the readership of the Journal for any inconvenience caused. [International Journal of Molecular Medicine 42: 2891­2902, 2018; DOI: 10.3892/ijmm.2018.3858].

Low-dose LPS alleviates early brain injury after SAH by modulating microglial M1/M2 polarization via USP19/FOXO1/IL-10/IL-10R1 signaling.

BACKGROUND: Low-dose lipopolysaccharide (LPS) protects against early brain injury (EBI) after subarachnoid hemorrhage (SAH). However, the mechanism underlying the neuroprotective roles of low-dose LPS remain largely undefined. METHODS: A SAH mice model was established and the pathological changes of brain were evaluated by wet-dry weight method, HE and Nissl staining, and blood-brain barrier (BBB) permeability assay. Cell apoptosis and inflammation were monitored by TUNEL, flow cytometry and ELISA assays. qRT-PCR, immunofluorescence and Western blot were used to detect the expression of microglial polarization-related or oxidative stress-associated markers. Bioinformatics analysis, luciferase and ChIP assays were employed to detect the direct association between FOXO1 and IL-10 promoter. The ubiquitination of FOXO1 in the in vitro SAH model was detected by co-IP. RESULTS: Low-dose LPS alleviated SAH-induced neurological dysfunction, brain edema, BBB disruption, damage in the hippocampus, neuronal apoptosis and inflammation via modulating microglial M1/M2 polarization by IL-10/IL-10R1 signaling. Mechanistic studies showed that FOXO1 acted as a transcriptional activator of IL-10. USP19 mediated the deubiquitination of FOXO1 to activate IL-10/IL-10R1 signaling, thereby regulating microglial M1/M2 polarization. Functional experiments revealed that low-dose LPS upregulated USP19 to modulate microglial M1/M2 polarization via FOXO1/IL-10/IL-10R1 signaling in SAH mice. CONCLUSION: Low-dose LPS protected against EBI after SAH by modulating microglial M1/M2 polarization via USP19/FOXO1/IL-10/IL-10R1 signaling.

Analysis of Risk Factors for Epilepsy after Combined Re-Vascularization in Adult Patients with Moyamoya Disease.

Background and Objective: Re-vascularization is an effective treatment for moyamoya disease (MMD) patients, including direct re-vascularization, indirect re-vascularization and combined re-vascularization, in which combined re-vascularization is particularly widely used. At present, there are few reports on the analysis of epilepsy after combined re-vascularization surgery. To analysis the risk factors of epilepsy in adult MMD patients after combined re-vascularization. Material and Methods: Patients with MMD who underwent combined re-vascularization in the Department of Neurosurgery of the First People's Hospital of Yunnan Province from January 2015 to June 2020 were included. Their pre-operative and post-operative complication-related indicators were collected. Finally, logistic regression was used to analyze the clinical risk factors of epilepsy in MMD patients after operation. Results: The incidence of epilepsy after combined re-vascularization was 15.5%. Univariate analysis showed that pre-operative ischemic or hemorrhagic stroke, pre-operative epilepsy, pre-operative history of diabetes, the location of the bypass recipient artery (frontal or temporal lobe), post-operative new cerebral infarction, hyper-perfusion syndrome, and post-operative intra-cranial hemorrhage were the clinical risk factors of epilepsy in MMD patients (all P < 0.05). Multi-variate logistic regression analysis showed that pre-operative epilepsy, the location of the bypass recipient artery, new cerebral infarction, hyper-perfusion syndrome, and post-operative intra-cranial hemorrhage (all P < 0.05) were independent risk factors for post-operative epilepsy in MMD patients. Conclusions: Pre-operative epilepsy, the location of the bypass recipient artery, new cerebral infarction, hyper-perfusion syndrome, and intra-cranial hemorrhage may have a causal relationship with epilepsy in adult MMD patients. It is suggested that some risk factors could be intervened to reduce the incidence of post-operative epilepsy in MMD patients.

Clinical value of the systemic immune-inflammation index in moyamoya disease.

Background: Moyamoya disease (MMD) is a rare cerebrovascular disorder with unknown etiology. The underlying pathophysiological mechanism of moyamoya disease remains to be elucidated, but recent studies have increasingly highlighted that abnormal immune response may be a potential trigger for MMD. Neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and systemic immune-inflammation index (SII) are inflammatory markers that can reflect the immune-inflammation state of the disease. Objective: The purpose of this study was to investigate SII, NLR, and PLR in patients with moyamoya disease. Methods: A total of 154 patients with moyamoya disease (MMD group) and 321 age- and sex-matched healthy subjects (control group) were included in this retrospective case-control study. Complete blood count parameters were assayed to calculate the SII, NLR, and PLR values. Results: The SII, NLR, and PLR values in the moyamoya disease group were significantly higher than those in the control group [754 ± 499 vs. 411 ± 205 (P < 0.001), 2.83 ± 1.98 vs. 1.81 ± 0.72 (P < 0.001), and 152 ± 64 vs. 120 ± 42 (P < 0.001), respectively]. The SII in the medium-moyamoya vessels of moyamoya disease was higher than that in the high-moyamoya vessels and low-moyamoya vessels (P = 0.005). Using the receiver operating characteristic (ROC) curve analysis to predict MMD, the highest area under the curve (AUC) was determined for SII (0.76 for SII, 0.69 for NLR, and 0.66 for PLR). Conclusion: Based on the results of this study, patients with moyamoya disease admitted for inpatient care due to acute or chronic stroke have significantly higher SII, NLR, and PLR when compared to blood samples drawn from completely healthy controls in a non-emergent outpatient setting. While the findings may suggest that inflammation plays a role in moyamoya disease, further studies are warranted to corroborate such an association. In the middle stage of moyamoya disease, there may be a more intense imbalance of immune inflammation. Further studies are needed to determine whether the SII index contributes to the diagnosis or serves as a potential marker of an inflammatory response in patients with moyamoya disease.

Clinical outcomes of pipeline embolization devices with shield technology for treating intracranial aneurysms.

Introduction: As a common endovascular treatment for intracranial aneurysms, the pipeline embolization device (PED) is considered a standard treatment option, especially for large, giant, wide-necked, or dissecting aneurysms. A layer of phosphorylcholine biocompatible polymer added to the surface of the PED can substantially improve this technology. This PED with shield technology (pipeline shield) is relatively novel; its early technical success and safety have been reported. We conducted a systematic literature review with the aim of evaluating the efficacy and safety of the pipeline shield. Methods: We searched the PubMed, Embase, and Cochrane databases, following the preferred reporting items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. Results: We selected five prospective and two retrospective studies for review. A total of 572 aneurysms were included; of these, 506 (88.5%) were unruptured. The antiplatelet regimens were heterogeneous. The rate of perioperative and postoperative complications was 11.1% [95% confidence interval (CI): 6.5-18.9%]. The adequate occlusion rate at 6 months was 73.9% (95% CI: 69.1-78.7%). The adequate occlusion rate of more than 12 months was 80.9% (95% CI: 75.1-86.1%). The mortality rate was 0.7% (95% CI: 0.2-1.5%). Subgroup analyses showed that aneurysm rupture status had no effect on aneurysm occlusion rate, patient morbidity, or mortality. Conclusion: This review demonstrates the safety and efficacy of the pipeline shield for treating intracranial aneurysms. However, direct comparisons of the pipeline shield with other flow diverters are needed to better understand the relative safety and effectiveness of different devices.

Iron-sulphur transformation control for enhancing Cr(VI) removal in flake and nanoscale porous pyrrhotite (Fe7S8) added wastewater.

Hexavalent chromium (Cr(VI)) contaminated wastewater should be addressed efficiently in the environmental field. In previous applications, nano iron sulfides amendment has not been well controlled for iron-sulfur transformation. In this study, the novel flake and nanoscale porous pyrrhotite (Fe7S8) (FNPP) amendment was synthesized. The iron-sulphur transformation of FNPP was controlled and optimized for enhancing Cr(VI) removal. The specific surface area and average pore diameter of the FNPP amendment reached 115.7 m2/g and 2.1 nm. The maximum adsorption capacity of total chromium reached 66.3 mg/g. The optimized iron-sulphur transformation condition was an initial FNPP and Cr(VI) molar ratio of 8, pH at 5.6, in which the Cr(VI) removal reached 96.5% and all producing S2- was utterly consumed. It is confirmed that S2- fast induced Fe3+/Fe2+ circulation and FNPP has a speedier adsorption rate for Cr(III) than Cr(VI). Fe2+ and S2- mediated the Cr(VI) reduction to Cr(III), thus, much faster Cr(VI) removal was achieved. High efficiency removal mechanism of Cr(VI) was combined with surface adsorption/reduction and solution reduction/precipitation. The research demonstrated that controlling and optimizing the iron-sulphur transformation of Fe7S8 amendment can significantly enhance Cr(VI) removal.

Humic acid promoted activation of peroxymonosulfate by Fe3S4 for degradation of 2,4,6-trichlorophenol: An experimental and theoretical study.

Chlorophenols are difficult to degrade and biohazardous in the natural environment. This study demonstrated that humic acid (HA) could promote Fe3S4 activation of peroxymonosulfate (PMS) to degrade 2,4,6-trichlorophenol (TCP), the degradation efficiency of TCP was increased by 33%. The system of Fe3S4-HA/PMS produced more reactive oxygen species, and •OH was the dominant ROS. The genealogy of iron oxides together with S0 on the Fe3S4 surface inhibited PMS activation leading to the significant reduction of TCP degraded (< 70%). These problems could be solved successfully through introducing HA, which facilitated electron transfer and increased the continuous release of iron ions by 2 times. In accordance with the determined density functional theory (DFT), the degradation pathway was put forward, which indicated that TCP dechlorination and oxidation to 2,6-dichloro-1,4-benzoquinone constituted the main degradation pathway. Furthermore, the intermediates that were produced in the main degradation processes of TCP showed lower toxicity than TCP according to results that were obtained utilizing the calculations of quantitative structure-activity relationship (QSAR) together with Toxicity Estimation Software Tool (TEST). Thus, the Fe3S4-HA/PMS system was demonstrated to be an efficient and safe technology for organic pollutant degradation in contaminated groundwater and surface water environments.

Chronic exposure to UV-aged microplastics induces neurotoxicity by affecting dopamine, glutamate, and serotonin neurotransmission in Caenorhabditis elegans.

Microplastics are ubiquitous in all environments and exert toxic effects in various organisms. However, the neurotoxicity and underlying mechanisms of long-term exposure to MPs aged under UV radiation remain largely unclear. In this study, Caenorhabditis elegans was treated with 0.1-100 µg/L virgin and aged polystyrene microplastics (PS-MPs) for 10 d, with locomotion behavior, neuronal development, neurotransmitter content, and neurotransmission-related to gene expression as endpoints. Using locomotion behavior as an endpoint, chronic exposure to aged PS-MPs at low concentrations (1 µg/L) caused more severe neurotoxicity than that to virgin PS-MPs. In transgenic nematodes, exposure to 10-100 µg/L aged PS-MPs significantly influenced the fluorescence intensity and percentage of worms with neurodegeneration of dopaminergic, glutamatergic, and serotonergic neurons compared with control. Further investigations showed that the content of glutamate, serotonin, and dopamine was significantly influenced in nematodes chronically exposed to 100 µg/L of aged PS-MPs. Similarly, neurotransmission-related gene (e.g., eat-4, dat-1, and tph-1) expression was also altered in nematodes. These results indicate that aged PS-MPs exert neurotoxicity owing to their effects on dopamine, glutamate, and serotonin neurotransmission. This study provides insights into the underlying mechanisms and potential risks of PS-MPs after UV radiation.

ETS1-activated SNHG10 exerts oncogenic functions in glioma via targeting miR-532-3p/FBXL19 axis.

BACKGROUND: In past few years, long non-coding RNAs (lncRNAs) have been reported to play regulatory roles during cancer progression. LncRNA SNHG10 has been explored in several sorts of cancers. However, its detailed role and mechanism are still not well understood in glioma. METHODS: Expression levels of genes were evaluated by RT-qPCR. EdU, TUNEL, sphere formation, wound healing and transwell assays appraised the effect of SNHG10 on glioma cellular processes. The interaction between molecules was examined by ChIP, RIP, RNA pull down and luciferase reporter assays. RESULTS: High level of SNHG10 was detected in glioma cells. Functional assay confirmed that SNHG10 promoted the proliferation, migration, invasion and stemness of glioma cells. Moreover, miR-532-3p was validated to bind with SNHG10 and expressed at a low level in glioma cells. Importantly, miR-532-3p exerted inhibitory functions in glioma. Furthermore, it was found that FBXL19 targeted by miR-532-3p facilitated cell growth and stemness in glioma, and that SNHG10 worked in glioma by increasing FBXL19 expression through sequestering miR-532-3p. More importantly, ETS1 promoted the transcription of SNHG10 and it mediated contribution to the malignant behaviors of glioma cells by SNHG10/miR-532-3p/FBXL19 signaling. CONCLUSION: SNHG10 was transcriptionally activated by ETS1 and played an oncogenic role in glioma by sponging miR-532-3p and up-regulating FBXL19.

Magnetic effervescent tablet-assisted ionic liquid-based dispersive liquid-liquid microextraction of polybrominated diphenyl ethers in liquid matrix samples.

Herein, a novel method, magnetic effervescent tablet-assisted ionic liquid-based dispersive liquid-liquid microextraction (META-IL-DLLME), was pioneered for extraction and preconcentration of polybrominated diphenyl ethers (PBDEs) in liquid matrix samples. In this proposed method, a magnetic effervescent tablet, containing CO2 sources, ionic liquids and Fe3S4 magnetic nanoparticles (MNPs), combines extractant dispersion and magnetic recovery into one-step. Fe3S4 was synthesized, characterized and applied it for the first time to the newly developed method, and its extraction recoveries (ERs) for PBDEs were 20.8-32.0% higher than those of conventional Fe3O4 MNPs. The increased ERs of Fe3S4 resulted from its larger specific surface area and pore size. Some important parameters were rigorously optimized, such as kinds of magnetic nanoparticles, effervescent agents, extraction solvents and their volumes, elution solvents, extraction temperature and salt addition. Under the optimized conditions, the META-IL-DLLME method combined with HPLC-DAD analysis gave the linear ranges of 0.1-0.5-100 µg L-1 with correlation coefficients of > 0.9990. The ERs ranged from 80.7% to 99.3%, and the limits of detection and quantitation were 0.012-0.078 µg L-1 and 0.04-0.26 µg L-1, respectively. The intra- and inter-day precisions, expressed as relative standard deviations (RSD, n = 6), were 1.32-4.83% and 1.99-4.25%, respectively. To evaluate its matrix effect, the relative recoveries of PBDEs from tap and river water, skim and whole milk, pregnant women and women serum samples at three fortification levels (2.0, 5.0 and 20.0 µg L-1) were in the range of 77.3-106.7%. Overall, the commercial Fe3O4 MNPs can only be used for magnetic separation in microextraction procedures, while Fe3S4 MNPs gave the higher adsorption and extraction efficiency for organic analytes besides the convenient magnetic separation. Therefore, the results obtained in this study provide a superior alternative for the conventional magnetic separation and adsorbent material. Also, this newly developed method has a great potential in routine monitoring of liquid matrix samples.

Bax inhibitor-1 suppresses early brain injury following experimental subarachnoid hemorrhage in rats.

Early brain injury (EBI) following subarachnoid hemorrhage (SAH) is an important cause of high mortality and poor prognosis in SAH. B­cell lymphoma 2­associated X protein inhibitor­1 (BI­1) is an evolutionarily conserved antiapoptotic protein that is primarily located in the membranes of endoplasmic reticulum (ER). BI­1 has been studied in certain nervous system­associated diseases, but the role of this protein in SAH remains unclear. In the present study, the role of BI­1 in EBI following SAH was investigated in rat models and its associated mechanisms were examined. The SAH rat model was generated by inserting nylon cords into the internal carotid artery from the external carotid artery. Samples were assessed using neurological scores, brain water content measurements, hematoxylin and eosin (H&E) staining, blood­brain barrier (BBB) permeability, terminal deoxynucleotidyl transferase­mediated dUTP nick­end labeling and quantitative polymerase chain reaction assays, and western blot analyses. It was identified that the mRNA and protein levels of BI­1 decreased markedly and were lowest at 24 h after SAH. BI­1 overexpression and small hairpin RNA (shRNA)­mediated silencing markedly suppressed or severely exacerbated EBI following SAH, respectively. BI­1 overexpression in the SAH model improved neurological scores and decreased the brain water content, BBB permeability and levels of apoptosis compared with the control and sham groups following SAH. BI­1 shRNA in the SAH model demonstrated contrary results. In addition, the mRNA or protein expression levels of ER stress­associated genes (glucose regulated protein, 78 kDa, C/EBP homologous protein, Serine/threonine­protein kinase/endoribonuclease IRE1, c­Jun N terminal kinases and apoptotic signaling kinase­1) were markedly suppressed or increased following BI­1 overexpression and shRNA­mediated silencing, respectively. The present study suggested that BI­1 serves a neuroprotective role in EBI following SAH by attenuating BBB disruption, brain edema and apoptosis mediated by ER stress.

Inhibitory effects of natural organic matter on methyltriclosan photolysis kinetics.

This study evaluated the effects and related mechanisms of natural organic matter (NOM) on the photolysis of methyltriclosan (MTCS), a metabolite of triclosan. Addition of two representative NOM isolates, Pony Lake fulvic acid (PLFA-microbial origin) and Suwannee River fulvic acid (SRFA-terrestrial origin), significantly inhibited the direct photolytic rate of MTCS by ∼70%. The MTCS photolytic rate in the presence of PLFA was greater than for SRFA. NOM not only suppressed photolysis by light-shielding, but also produced ROS to oxidatively degrade MTCS and/or triplet NOM (3NOM*) to sensitize degradation. The dual effects of light-screening and photo-sensitization led to an overall decrease in photolysis of MTCS with a positive concentration-dependence. Upon addition of NOM, EPR documented the occurrence of 1O2 and ˙OH in the photolytic process, and the bimolecular k value for the reaction of 1O2 with MTCS was 1.86 × 106 M-1 s-1. ROS-quenching experiments indicated that the contribution of ˙OH (19.1-29.5%) to indirect photolysis of MTCS was lower than for 1O2 (38.3-58.7%). Experiments with D2O further demonstrated that 1O2 participated in MTCS photodegradation. Moreover, the addition of sorbic acid and O2 gas to the reaction confirmed the participation of 3NOM* as a key reactant in the photochemical transformation of MTCS. This is the first comprehensive analysis of NOM effects on the indirect photolysis of MTCS, which provides new insights for understanding the environmental fate of MTCS in natural environments.

Salting-out-enhanced ionic liquid microextraction with a dual-role solvent for simultaneous determination of trace pollutants with a wide polarity range in aqueous samples.

In real aquatic environments, many occupational pollutants with a wide range of polarities coexist at nanogram to milligram per liter levels. Most reported microextraction methods focus on extracting compounds with similar properties (e.g., polarity or specific functional groups). Herein, we developed a salting-out-enhanced ionic liquid microextraction based on a dual-role solvent (SILM-DS) for simultaneous detection of tetracycline, doxycycline, bisphenol A, triclosan, and methyltriclosan, with log K ow ranging from -1.32 to 5.40 in complex milk and environmental water matrices. The disperser in the ionic-liquid-based dispersive liquid-liquid microextraction was converted to the extraction solvent in the subsequent salting-out-assisted microextraction procedures, and thus a single solvent performed a dual role as both extractant and disperser in the SILM-DS process. Acetonitrile was selected as the dual-role solvent because of its strong affinity for both ionic liquids and water, as well as the extractant in the salting-out step. Optimized experimental conditions were 115 µL [C8MIM][PF6] as extractor, 1200 µL acetonitrile as dual-role solvent, pH 2.0, 5.0 min ultrasound extraction time, 3.0 g Na2SO4, and 3.0 min vortex extraction time. Under optimized conditions, the recoveries of the five pollutants ranged from 74.5 to 106.9%, and their LODs were 0.12-0.75 µg kg-1 in milk samples and 0.11-0.79 µg L-1 in environmental waters. Experimental precision based on relative standard deviation was 1.4-6.4% for intraday and 2.3-6.5% for interday analyses. Compared with previous methods, the prominent advantages of the newly developed method are simultaneous determination of pollutants with a wide range of polarities and a substantially reduced workload for ordinary environmental monitoring and food tests. Therefore, the new method has great application potential for simultaneous determination of trace pollutants with strongly contrasting polarities in several analytical fields. Graphical Abstract A salting-out-enhanced ionic liquid microextraction based on a dual-role solvent (SILM-DS) was developed for simultaneous detection of tetracycline, doxycycline, bisphenol A, triclosan and methyltriclosan, with log K ow ranging from -1.32 to 5.40. The novelty of SILM-DS method lies in (1) simultaneous quantification of pollutants with contrasting polarity; (2) microextraction based on a dual-role solvent (as a disperser and extractant); (3) giving high recoveries for analytes with a wide range of polarities; and (4) reducing workload for ordinary environmental monitoring and food tests.