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OBJECTIVE: Toothpastes are widely used to protect oral and teeth health. This study aims to examine the cytotoxic and antimicrobial effects of whitening toothpastes. METHODS: In this study, extracts were prepared according to ISO 10993-12:2021 standard (0.2 g/mL) using whitening and conventional toothpastes. The prepared extracts were added to human gingival fibroblast cell lines (HGF-1) in different dilutions (1:1, 1:2, 1:4, 1:8, 1:16, and 1:32) and a cytotoxicity test was performed. Antimicrobial analysis of toothpastes was performed on Streptococcus mutans, Staphylococcus aureus, and Candida albicans using the hole-plate diffusion method. Cell viability and microbial analysis data were examined using two-way analysis of variance (ANOVA) and Tukey post-hoc test (p < 0.05). RESULTS: Toothpastes with sodium lauryl sulfate (SLS) in their composition showed statistically more toxic effects (p < 0.05). The activated carbon toothpastes without SLS showed over 90% cell viability after dilution. Although the dilution rate of toothpastes containing SLS increased, cell viability remained below 70%. All toothpastes used in the study showed antimicrobial effects on S. mutans, S. aureus, and C. albicans. Toothpaste containing hydrogen peroxide and SLS produced more antibacterial effects than activated carbon, blue covarine, microparticles, and conventional toothpaste. CONCLUSIONS: SLS-containing toothpastes showed more toxicity on HGF-1 cells. Toothpaste containing hydroxyapatite did not show toxic effects on HGF-1 cells. SLS, sodium lauryl sarcosinate and hydrogen peroxide in toothpastes increase antimicrobial effects.
Sujet(s)
Anti-infectieux , Candida albicans , Staphylococcus aureus , Streptococcus mutans , Pâtes dentifrices , Pâtes dentifrices/pharmacologie , Humains , Candida albicans/effets des médicaments et des substances chimiques , Staphylococcus aureus/effets des médicaments et des substances chimiques , Streptococcus mutans/effets des médicaments et des substances chimiques , Anti-infectieux/pharmacologie , Survie cellulaire/effets des médicaments et des substances chimiques , Lignée cellulaire , Techniques in vitro , Fibroblastes/effets des médicaments et des substances chimiques , Agents de blanchiment des dents/toxicité , Agents de blanchiment des dents/pharmacologieRÉSUMÉ
BACKGROUND: Information on the current status of oral health and related risk factors is lacking in Turkiye. This study aimed to evaluate dental caries and associated factors among the Turkish population using the findings of the third national oral health survey. METHODS: This cross-sectional epidemiological survey was carried out between March and September in 2018 including five index ages and age groups (5-, 12-, 15-year-olds, 35-44 and 65-74 age groups). A multistage cluster sampling design was used to obtain a national representative sample of these age groups. Participants were interviewed face-to-face using a structured questionnaire, and oral examinations were performed based on the methods and criteria suggested by the World Health Organization. The adjusted prevalence ratio for dental caries was estimated considering sociodemographic and behavioural characteristics by using log-binomial and robust Poisson regression analysis in R and SAS software. RESULTS: After data cleaning, a total of 11 091 participants remained. The prevalence of dental caries was 76.5% (95% confidence interval: 75.4%-77.7%) and determined to be associated with age, dental visit, and pain or discomfort history during the previous year among the Turkish population. Prevalence was also associated with the mother's education level among 5-, 12- and 15-year-olds. For the 35-44 age group, individuals living in urban areas had lower dental caries than those living in rural areas. For 65-74 age group, none of the factors were significantly associated with dental caries. CONCLUSION: A high prevalence of dental caries was observed in Turkish children and adults. Therefore, it is necessary to strengthen current preventive oral health programs. Regular disease surveillance should be maintained. National oral health targets should be formulated in accordance with Turkiye's needs, resources and structure.
Sujet(s)
Caries dentaires , Enquêtes de santé dentaire , Humains , Turquie/épidémiologie , Caries dentaires/épidémiologie , Adolescent , Enfant , Femelle , Mâle , Études transversales , Adulte , Enfant d'âge préscolaire , Prévalence , Facteurs de risque , Sujet âgé , Santé buccodentaire/statistiques et données numériquesRÉSUMÉ
Purpose: The aim of this study is to evaluate the amount of microleakage resulting from the application of self-etch, selective etch, etch-and-rinse of adhesive systems in class II cavities. Materials and methods: Four adhesive systems with etch-and-rinse, selective etch and self etch methods were used on the extracted teeth. All groups were restored with G-aenial A'CHORD (Nanohybrid) (GC, Tokyo, Japan) A2 composite. After 1000 thermal cycles were applied to the teeth after restoration, the samples were kept in 0.5% basic fuchsin for 24 hours. Microleakage values formed after dye penetration with basic fuchsin were determined quantitatively by scoring method on sections taken from each sample in the mesiodistal direction. One-way Analysis of Variance (ANOVA) and Tukey test were used for statistical analysis of the data (p<0.05). Results: While there was no statistically significant difference between the etch-and-rinse and selective etch applications of adhesive systems (G2-Bond Universal, Clearfil Tri-S Bond Universal Prime&Bond Universal and Tokuyama Bond Force II) (p>0.05), there was a statistically significant difference in self-etch application (p<0.05). As a result of Prime&Bond Universal's self-etch application, it showed statistically more microleakage than the other three adhesive systems (p<0.05). Conclusion: It has been observed that additional etching of enamel and/or dentin with phosphoric acid reduces the amount of microleakage.
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Purpose: This study aimed to examine the effect of beverages at different temperatures on the coloring of composites. Materials and methods: A total of 48 cylindrical samples, 24 of which were prepared from 2 composite materials (G'aenial; Estelite Σ Quick), were included in the study. The sample dimensions were standardized at 2 x 10 mm2. After the polishing, the initial color measurements were performed using a spectrophotometer. The samples were divided into 3 subgroups as distilled water, hot coffee (60 °C) and cold coffee (0 °C) (n=8). During the 7th and 30th days, the samples were immersed in the solutions for 15 min every day. Color measurements were repeated on the 7th and 30th days. Data were analyzed using a two-way analysis of variance, followed by the Tukey post-hoc test (p<0.05). Results: The highest color change was detected on the 7th and 30th days in the G-aenial anterior microhybrid composite immersed in hot coffee (p<0.001). The application of hot and cold coffee applications did not make a statistically significant difference in the coloration of the Estelite Σ Quick composite samples at the end of the 7th (p=0.346) and 30th (p=910) days. Conclusion: Hot drinks had a more coloring effect on restorations. This coloration was quite evident in the microhybrid composite.
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Background: This study investigated the cytotoxic effects of 3D-printed permanent resins, resin-based CAD/CAM blocks and composite resin on human gingival fibroblast (HGF-1) and mouse fibroblast (L929) cell line. Material and Methods: 3D-printed permanent resins (Crowntec and Permanent Crown), resin-based CAD/CAM blocks (Vita Enamic and Brilliant Crios) and composite resin (Clearfill Majesty Posterior) were used in the study. Samples were prepared from the planned materials and kept in DMEM according to ISO 10993-12:2021 standard (3 cm2/ml). The cytotoxic effect of the materials on HGF-1 and L929 cells was examined by MTT test at the end of 24 and 72 h. Two-way analysis of variance test (ANOVA) was used to analyze cell viability data. Results: 3D-printed permanent resins, resin-based CAD/CAM blocks and composite resin extracts showed similar cell viability on HGF-1 and L929 cells at the end of 24 h (p>0.05). Resin-based CAD/CAM block (Vita Enamic) produced the highest cell viability on HGF-1 and L929 cells at the end of 72 h (p<0.05). Cell viability values of samples produced in 3D printers with different printing properties did not differ significantly (p>0.05). Conclusions: 3D-printed permanent restoration resins showed similar cell viability on HGF-1 and L929 cells to resin-based CAD/CAM blocks and composite resin. Key words:3D-printed resin, CAD/CAM block, Composite resin, Cytotoxicity, Human Gingival Fibroblast.
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Abstract The desire of individuals to have whiter teeth increases the interest in tooth whitening products. Our aim was to in vitro study the whitening effect of hydrogen peroxide, blue covarine and active charcoal containing whitening toothpastes on human teeth. A total of 40 extracted human incisor teeth were used in the study. To measure the whitening effect of toothpastes, the teeth were divided into four subgroups and placed in the phantom tooth jaw model. Then, daily brushing was done with an electric toothbrush. The colors of the teeth were measured initially using the spectrophotometer (single point and bleached shade mode) and at the end of 7th, 14th and 28th days. Whitening effectiveness of toothpastes were studied according to CIEDE2000 formula (ΔE00) and shade guide units (SGU). One- way analysis of variance (ANOVA) and Tukey test were used in the statistical analysis of the data. (p0.05). Blue covarine containing toothpaste had statistically the lowest whitening effect (p<0.05). All toothpastes showed a whitening effect on the teeth after 7 days of use. Activated charcoal containing toothpaste showed more whitening effect after 28 days of use than hydrogen peroxide, blue covarine and traditional toothpaste.
Resumen El deseo de los individuos de tener unos dientes más blancos aumenta el interés por los productos de blanqueamiento dental. Nuestro objetivo fue estudiar el efecto blanqueador de las pastas dentales blanqueadoras que contienen peróxido de hidrógeno, covarina azul y carbón activo en dientes humanos in vitro. En el estudio se utilizaron un total de 40 dientes incisivos humanos extraídos. Para medir el efecto blanqueador de los dentífricos, los dientes se dividieron en cuatro subgrupos y se colocaron en el modelo de diente fantasma en mandíbula. A continuación, se realizó un cepillado diario con un cepillo eléctrico. El color de los dientes se midió inicialmente con un espectrofotómetro (modo de punto único y tono blanqueado) y al final de los días 7, 14 y 28. Se estudió la eficacia blanqueadora de los dentífricos según la fórmula CIEDE2000 (ΔE00) y las unidades de guía de color (SGU). En el análisis estadístico de los datos se utilizó el análisis de varianza de una vía (ANOVA) y la prueba de Tukey. (p0,05). El dentífrico que contiene covarina azul tuvo estadísticamente el menor efecto blanqueador (p<0,05). Todos los dentífricos mostraron un efecto blanqueador en los dientes después de 7 días de uso. Los dentífricos con carbón activado mostraron un mayor efecto blanqueador tras 28 días de uso que el peróxido de hidrógeno, la covarina azul y el dentífrico tradicional.
Sujet(s)
Blanchiment dentaire , Dentifrices , Peroxyde d'hydrogène/analyseRÉSUMÉ
Background: The aim of our study is to assess the dose enhancement from scattered radiation due to dental restorative materials used for occlusal and mesio-occlusal-distal (MOD) cavity filling during simulated head and neck radiotherapy. Methods: We have studied the dose enhancement ratio (DER) of conventional amalgam, high-copper amalgam, and resin composite dental restorative materials at cadaver mandible teeth using 2 therapeutic photon energies of 1.25 MeV (Co-60 gamma ray) and 6 MV (Linac X-ray) for irradiation. Results: DER values at buccal position for Co-60 and 6 MV X-ray were 1.250 ± 0.013 and 1.151 ± 0.012, respectively. For dental cavity fillings, DER values for 6 MV X-ray were 1.065 ± 0.021, 1.100 ± 0.014, and 1.162 ± 0.016 for resin composite filling, low-copper amalgam filling, and high-copper amalgam filling, respectively. Our results revealed that DER regarding irradiation energy was minimum for 6 MV X-rays. With respect to dental restorative filling material, DER was minimum for resin composite filling. Regarding the cavity type, our results with standard deviation (SD) calculations revealed that DER was slightly but not significantly different for both Co-60 gamma ray (1.25 MeV) and 6 MV X-ray energies for both occlusal and MOD cavities. Conclusion: Our dosimetric results for a single beam geometry suggest that, among the three types of filling, resin composite filling is an ideal restorative filling material with minimal morbidity-inducing radiation dose enhancement that may result in increased osteoradionecrosis and secondary caries risk. There is a need for further dosimetric studies with actual clinical beam arrangements.
Sujet(s)
Radio-isotopes du cobalt , Cuivre , Humains , Radio-isotopes du cobalt/usage thérapeutique , Radiométrie , Résines composites/usage thérapeutiqueRÉSUMÉ
ABSTRACT: CAD/CAM blocks are widely used in dental restorations around the world. This study aimed to investigate the effects of different polishing and finishing systems on surface roughness and color change of resin-based CAD/CAM blocks. In this study, material samples of 2×7×12mm were prepared. In three different experimental groups, surface polishing was carried out with one-step, two-step and multi-step polishing systems. The samples in one subgroup of each experimental group were polished with the diamond paste. Once the initial roughness and color values of the samples were measured using a profilometer and a spectrophotometer, the samples were immersed in coffee. Surface roughness and color change (ΔE00) results were statistically analyzed using two-way variance analysis (ANOVA). Finishing and polishing systems created significantly different levels of surface roughness on the hybrid ceramic block (Vita Enamic) but not on the composites block (Brilliant Crios, Grandio Blocs). While the two-step and multi-step finishing and polishing systems produced the lowest color change on hybrid ceramic blocks, one-step and two-step systems achieved this on composite blocks (p<0.05). In all test groups, the supplementary polishing performed after regular polishing procedures helped us reduce the surface roughness and color change on CAD/CAM blocks. The findings obtained in this study suggest that one and two-step polishing systems are more suitable for resin-based composite CAD/CAM blocks; for hybrid ceramic CAD/CAM blocks, on the other hand, two and multi-step finishing and polishing systems seem to be more efficient concerning lower surface roughness and resistance to color changes.
RESUMEN: Los bloques CAD/CAM son ampliamente utilizados en las restauraciones dentales, alrededor del mundo. El objetivo de este estudio es investigar los efectos de diferentes sistemas de pulido y acabado sobre la rugosidad de la superficie y el cambio de color de los bloques CAD/CAM de resina. En este estudio se prepararon muestras de material de 2×7×12mm. El pulido de la superficie se llevó a cabo en tres grupos experimentales con sistemas de pulido de un paso, de dos pasos y de varios pasos. Las muestras de un subgrupo de cada grupo experimental se pulieron con la pasta de diamante. Una vez medidos los valores iniciales de rugosidad y color de las muestras mediante un perfilómetro y un espectrofotómetro, las muestras se sumergieron en café. Los resultados de la rugosidad superficial y el cambio de color (ΔE00) se analizaron estadísticamente mediante un análisis de varianza de dos vías (ANOVA). Los sistemas de acabado y pulido crearon niveles de rugosidad superficial significativamente diferentes en el bloque cerámica híbrida (Vita Enamic) pero no en el bloque de composites (Brilliant Crios, Grandio Blocs). Mientras que los sistemas de acabado y pulido de dos y varios pasos produjeron el menor cambio de color en los bloques de cerámica híbrida, los sistemas de un paso y dos pasos lo consiguieron en los bloques de composite (p<0,05). En todos los grupos de prueba, el pulido suplementario realizado después de los procedimientos de pulido regulares nos ayudó a reducir la rugosidad de la superficie y el cambio de color en los bloques CAD/CAM. Los hallazgos obtenidos en este estudio sugieren que los sistemas de pulido de uno y dos pasos son más adecuados para los bloques CAD/CAM de resina; en cambio, para los bloques CAD/CAM de cerámica híbrida, los sistemas de acabado y pulido de dos y varios pasos parecen ser más eficientes en lo que respecta a la menor rugosidad de la superficie y la resistencia a los cambios de color.
Sujet(s)
Résines synthétiques , Couleur , Restaurations dentaires permanentes/instrumentationRÉSUMÉ
BACKGROUND: This study is to examine the surface roughness and color changes occurring on composite resins following the application of different finishing and polishing systems. MATERIAL AND METHODS: In our study, a total of 200 samples were prepared from composites resin (6×2 mm) containing supra-nano, submicron hybrid, nanohybrid, nano-ceramic and microhybrid filler. They were polished with diamond, aluminum oxide, silicon carbide finishing and polishing systems. The initial color values of composite samples were measured with a spectrophotometer and surface roughness values with a profilometer. After that, samples were immersed of coffee solution and color measurements were repeated on the 1st and 7th day. The differences between surface roughness (Ra) and color change values (∆Eab) were evaluated using two-way analysis of variance (ANOVA) test (p<0.05). RESULTS: Finishing and polishing systems produced a statistically significant difference between the surface roughness values of nano-ceramic, submicron hybrid and microhybrid composite resin samples (p<0.05). Supra nano composite, which showed the lowest surface roughness after all finishing and polishing systems, showed the least color change after 7 days compared to other composite groups (p<0.05). The microhybrid composite with the highest surface roughness was the most color changing composite group (p<0.5). CONCLUSIONS: The spiral finishing and polishing system containing diamond particles was the system to provide the least color difference on all composite groups. However, color differences of all composite resin groups were found to exceed the perceptibility threshold (PT) and acceptability threshold (AT). Key words:Composite resin, color stability, surface roughness.
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INTRODUCTION: Some colorant foods and beverages cause undesired discoloration on dental restorations. The aim of this study was to investigate the effect of whitening kinds of toothpaste with different active ingredients on the color change of resin-based CAD/CAM blocks. MATERIAL AND METHODS: In the study, resin-based CAD/CAM block samples sized 1.5 × 7 × 12 mm, were prepared. Then, to simulate the time-dependent color change, the samples were immersed in coffee (Nescafe Classic, Nestlé) for 14 days. After the color change values of the samples colored in coffee were measured by spectrophotometer, the samples were brushed with different types of whitening toothpastes for 4 minutes. Statistical analysis of the color change (ΔE00 ) was performed using the one-way analysis of variance (ANOVA). RESULTS: Color differences occurring on all resin-based CAD/CAM blocks immersed in coffee for 14 days exceeded the 50%:50% perceptibility threshold (PT) (ΔE00 :0.8). There was no statistical significant difference between the whitening toothpastes containing activated charcoal, hydrogen peroxide (HP), blue covarine pigment, and microparticles in color improvement on resin-based CAD/CAM blocks (P > .05). CONCLUSION: It has been verified that the discoloration on resin-based CAD/CAM blocks exhibiting color difference above the PT after coffee immersion can be reduced by utilizing toothpastes containing whiteners. CLINICAL SIGNIFICANCE: Color changes that occur over time in resin-based CAD/CAM blocks can be a clinical problem. These color changes in the materials can be reduced below the PT level using whitening toothpastes.
Sujet(s)
Conception assistée par ordinateur , Pâtes dentifrices , Couleur , Test de matériaux , Spectrophotométrie , Propriétés de surfaceRÉSUMÉ
Objective: Unlike traditional composite resins, bulk-fill composite resins could be polymerized as thicker layers. This study aims to contribute to the field by investigating the cytotoxic effects of various bulk-fill composite resins on L929 mouse fibroblast cells in vitro. Material and Methods: In our study, six bulk fill and one conventional composite resin were used. Composite resin samples (8×4 mm) were prepared in a sterile cabinet by using a glass mod and polymerizing with a led light device (DTE LUX E, Germany). Composite samples (n:3) of which surface area was calculated according to ISO 10993-12: 2012 standards (3 cm2/ml), were kept in media for 24 h and 72 h in 37 oC incubator, their extracts were filtered in 1:1 and 1:2 proportion and were added on L929 mouse fibroblast cells. Cell viability was examined by the MTT assay and cell death by the LDH test. Cell viability results were evaluated using one-way analysis of variance (ANOVA) test (p<0.05). Results: When the 1:1 extracts from 4 mm thick bulk-fill composite samples were applied on L929 mouse fibroblast cells, cell viability rates showed significant differences compared to the control group at the end of 24 h and 72 h (except for Estelite Bulk Fill Flow). Although the extracts of the tested composite samples at 1:1 and 1:2 ratio at the end of 72 hours caused a decrease in L929 mouse fibroblast cell viability, the cell viability rate of only PRG-containing bulk fill composite and conventional composite remained below the cell viability ratio (70%) specified in ISO standards. Bulk fill composites did not produce toxic effects (except Beautifil Bulk Restorative) according to the LDH test. Conclusions: Despite decreasing in general the cell viability, bulk-fill composite resins used in 4 mm thick layers provided cell viability rates over the acceptability level, except PRG-containing bulk fill composite (Beautifil Bulk Restorative), which was cytotoxic to L929 mouse fibroblasts. (AU)
Objetivo: Ao contrário das resinas compostas tradicionais, as resinas compostas bulk-fill podem ser polimerizadas como camadas mais espessas. Este estudo visa investigar in vitro os efeitos citotóxicos de várias resinas compostas bulk-fill em células de fibroblastos de camundongo L929.Material e Métodos: Em nosso estudo, seis resinas tipo bulk fill e uma resina composta convencional foram usadas. Amostras de resina composta (8 × 4 mm) foram preparadas em gabinete estéril usando um molde de vidro e polimerizado com um dispositivo de luz LED (DTE LUX E, Alemanha). Amostras compostas (n=3) cuja área de superfície foi calculada de acordo com os padrões ISO 10993-12:2012 (3cm2/ml), foram mantidas em meio e incubadas por 24 h e 72 h a 37 ºC, seus extratos foram filtrados na Proporção de 1:1 e 1:2 e foram acondicionados em cultura de células de fibroblastos de camundongo L929. A viabilidade celular foi examinada pelo ensaio MTT e a morte celular pelo teste LDH. Os resultados de viabilidade celular foram avaliados usando o teste de análise de variância (ANOVA) um fator (p <0,05). Resultados: Quando os extratos foram plaqueados na proporção 1:1 de amostras de compósito bulk-fill de 4 mm de espessura com as células de fibroblastos de camundongo L929, as taxas de viabilidade celular mostraram diferenças significativas em comparação com o grupo controle no final de 24 h e 72 h (exceto para Estelite Bulk Fluxo de enchimento). Embora os extratos das amostras compostas testadas na proporção de 1:1 e 1:2 ao final de 72 horas tenham causado uma diminuição na viabilidade das células de fibroblastos de camundongo L929, a taxa de viabilidade celular apenas do compósito de preenchimento total contendo PRG e o compósito convencional permaneceram abaixo a taxa de viabilidade celular (70%) especificada nas normas ISO. Os compósitos de preenchimento a granel não produziram efeitos tóxicos (exceto Beautifil Bulk Restorative) de acordo com o teste de LDH. Conclusão: Apesar de diminuir em geral a viabilidade celular, as resinas compostas bulk-fill usadas em camadas de 4 mm de espessura forneceram taxas de viabilidade celular acima do nível aceitável, exceto o compósito bulk fill contendo PRG (Beautifil Bulk Restorative), que foi citotóxico para fibroblastos de camundongos L929 (AU)
Sujet(s)
Animaux , Souris , Résines composites , Toxicité , FibroblastesRÉSUMÉ
BACKGROUND: High viscosity glass ionomer cement (HVGIC) and resin-modified glass ionomer cement (RMGIC) have recently been clinically preferred thanks to their numerous advantages. However, initial moisture contamination has a negative effect on the mechanical and physical properties of these cements. The aim of this study was in vitro of HVGICs and RMGICs, with and without surface protection, on water sorption, solubility and release of aluminum. MATERIAL AND METHODS: In this study, as HVGICs; Equia Forte, IonoStar Plus, Riva Self Cure; as RMCIS, Ionolux and Riva Light Cure; and as control, Z250 universal composite was used. Equia coat, Voco varnish and Riva coat were chosen as surface protective. Water sorption and solubility levels of the samples were measured according to ISO 4049:2009. Al levels released from samples were determined by graphite furnace atomic absorption spectroscopy (GFAAS) for 7, 14 and 21 days. Statistical evaluation of the results was made using one-way variance analysis (ANOVA) and Tukey post-hoc test (p<0.05). RESULTS: RMGICs from restorative materials showed more water absorption than HVGICs, but no differences in solubility. Among the materials tested, the water absorption values of the HVGIC and RMGIC materials without surface protection were higher than those with the surface protection (p<0.001). CONCLUSIONS: It was determined that the Al release of HVGIC and RMGIC groups with the surface protection were lower in all time periods than the groups without surface protection (p<0.001). The application of surface protection effectively reduced water sorption and Al release from HVGICs and RMGICs. Key words:Highly viscous glass ionomer cement, resin coating, aluminum release, water sorption, solubility.
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OBJECTIVE: The improvement of the optical properties of new generation dental ceramics enables more esthetic and durable restorations. This study aimed to investigate the color changes, caused by exposure to different beverages on composite, hybrid ceramic, and zirconia-reinforced lithium silicate (ZLS) CAD/CAM blocks. MATERIALS AND METHODS: CAD/CAM blocks 40 samples (1.5 × 7 × 12 mm) were obtained from each material under water cooling. Following the polishing process and the initial color measurement, the prepared samples were exposed to red wine, coffee, cola, energy drink, and distilled water. Color changes were measured by spectrophotometer at the end of the 1st, 7th, and 30th days. To analyze the color change results, one-way analysis of variance (ANOVA) test was performed. RESULTS: In all restorative material groups, the most prominent color changes were observed on the red wine-exposed samples, then the coffee-exposed ones. No significant discoloration was observed on the samples exposed to cola and energy drink, compared to the control group (P > .05). Among the samples exposed to red wine and coffee, the highest discoloration values were observed in composite CAD/CAM material. CONCLUSIONS: At the end of the 30th day, all materials exposed to wine and coffee showed color change above the clinically acceptable value (ΔE00 :2.25). CLINICAL SIGNIFICANCE: Hybrid ceramic and ZLS CAD/CAM blocks recently used more often by clinicians have shown similar color changes. The results of our study indicate the necessity of choosing esthetic materials according to the dietary habits of individuals.
Sujet(s)
Céramiques , Conception assistée par ordinateur , Couleur , Résines composites , Matériaux dentaires , Porcelaine dentaire , Humains , Test de matériaux , Propriétés de surfaceRÉSUMÉ
Candida albicans biofilms are commonly associated with severe oral infections. In dentistry, prosthetic and restorative materials are potential structures for the adhesion of C. albicans facilitating the formation of Candida biofilm and infection. Three composite resins (Charisma Classic, Sonic Fill, Estelite ∑ Quick) and two finishing-polishing systems (Biscover LV, Dental Finishing Disc) were evaluated for Candida biofilm formation. A Candida biofilm assay showed that both the resin and the finishing/polishing procedures affect Candida biofilm formation. Specifically, Candida biofilm formation was significantly lower in Sonic Fill resin than both Charisma Classic and Estelite ∑ Quick (P = .021). The type of finishing and polishing procedure also significantly affected the Candida biofilm formation to composite material (P < .001). Candida biofilm formation was more advanced after Biscover LV procedure than Dental Finishing Disc procedure.
Sujet(s)
Biofilms/croissance et développement , Candida albicans/croissance et développement , Résines composites , Polissage dentaire , Adhérence cellulaire , Test de matériaux , Propriétés de surfaceRÉSUMÉ
Objective: Dental composites developed by using nanotechnology in the field of dentistry are widely used in the treatment of anterior and posterior teeth. This study aimed to investigate the cytotoxic effects of dental composites of different particle size on L929 mouse fibroblast cell line by extract test method in vitro. Material and Methods: Composite samples of 8 x 2 mm diameter were prepared by polymerizing with led light device by using glass mod in a sterile cabinet. Composite samples of which surface areas were calculated according to ISO standards (3 cm2 / ml), were incubated for 24 and 72 hours, at 37 o C. cell viability was assessed by 3-[4,5-dimethylthiazole-2- yl]-2,5-diphenyltetrazolium bromide (MTT) assay and cell death was evaluated by the lactate dehydrogenase (LDH) leakage assay. Results: The 1:1 extracts of the composites at the end of 24 hours (except for nanoceramic composite) showed no toxic effect. When the cell viability results of the 1:1 extracts of the composite samples at the end of 72 hours were statistically analyzed, significant differences were found comparing to the control group (p < 0.05). Conclusion: It was observed that the type and size of the filler were effective on the toxicity of the composites, and the composites containing Bis-GMA, TEGDMA, UDMA and Bis EMA monomers in their organic matrix showed acceptable cell viability (70%) as specified by ISO. However, the composites with PEGDMA and BPA monomers in their organic matrix showed poor cell viability, which is below the acceptable level of 70%, and were found to have a toxic effect. (AU)
Objetivo: As resinas compostas desenvolvidas pela nanotecnologia no campo da odontologia são amplamente utilizadas no tratamento de dentes anteriores e posteriores. Este estudo teve como objetivo investigar os efeitos citotóxicos de resinas compostas de diferentes tamanhos de partículas na linha celular de fibroblastos de camundongos L929 pelo método de teste de extrato in vitro. Material e Métodos: Amostras compostas de 8 x 2 mm de diâmetro foram preparadas por polimerização com dispositivo de luz led usando um molde de vidro em um gabinete estéril. Amostras de resinas cujas áreas de superfície foram calculadas de acordo com os padrões ISO (3 cm2 / ml), foram incubadas por 24 e 72 horas, a 37 o C. A viabilidade celular foi avaliada pelo ensaio de brometo de 3- [4,5-dimetiltiazol-2- il] -2,5-difeniltetrazólio (MTT) e a morte celular foi avaliada pelo ensaio de infiltração de lactato desidrogenase (LDH). Resultados: Os extratos 1: 1 dos compósitos ao final de 24 horas (exceto o composto nanocerâmico) não apresentaram efeito tóxico. Quando os resultados de viabilidade celular dos extratos 1: 1 das amostras compostas ao final de 72 horas foram analisados, estatisticamente, foram encontradas diferenças significativas em relação ao grupo controle (p < 0,05). Conclusão: Observou-se que o tipo e tamanho da carga foram eficazes na toxicidade dos compósitos, e os compósitos contendo os monômeros Bis-GMA, TEGDMA, UDMA e Bis EMA em sua matriz orgânica apresentaram viabilidade celular aceitável (70%) como especificado pela ISO. No entanto, os compósitos com monômeros PEGDMA e BPA em sua matriz orgânica apresentaram baixa viabilidade celular, que está abaixo do nível aceitável de 70%, e foram encontrados como tendo um efeito tóxico. (AU)
Sujet(s)
Animaux , Souris , Résines composites/toxicité , Dentisterie esthétique , Fibroblastes , Techniques in vitro , Lignée cellulaire , Survie cellulaire , Nanoparticules , L-Lactate dehydrogenase/toxicitéRÉSUMÉ
The purpose of this study was to evaluate the effectiveness of several surface protectors for a glass-ionomer, a resin-modified glass-ionomer, and a polyacid-modified resin cement by determining dye uptake spectrophotometrically. 378 samples, made up of Ionofil U, Vitremer, and Dyract, were prepared and divided into groups of seven each. Positive and negative control specimens remained unprotected while the experimental specimens were protected with Finishing Gloss, Protect-It, LC Varnish, Adper Single Bond, or a nail varnish. The experimental groups and positive controls were immersed in 0.05% methylene blue solution, while the negative controls were immersed in deionized water. Results were evaluated using variance analysis. Of the Ionofil U group, Adper Single Bond exhibited the least effective surface coating among the materials tested, while the best surface protection was obtained with LC Varnish in the Dyract group. However, no statistically significant differences were observed in the Vitremer group.
Sujet(s)
Compomères/composition chimique , Matériaux dentaires/composition chimique , Ciment ionomère au verre/composition chimique , Céments résine/composition chimique , Absorption , Méthacrylate bisphénol A-glycidyl/composition chimique , Camphre/composition chimique , Collodion/composition chimique , Agents colorants/composition chimique , Résines composites/composition chimique , Humains , Test de matériaux , Méthacrylates/composition chimique , Polyéthylène glycols/composition chimique , Poly(acides méthacryliques)/composition chimique , Spectrophotométrie , Sulfonamides/composition chimique , Propriétés de surface , Toluène/composition chimiqueRÉSUMÉ
OBJECTIVE: This study evaluated the effects of amalgam restorations on plasma mercury levels and total antioxidant activities (TAA). DESIGN: The study was comprised of 48 subjects ranging in age from 20 to 32 years. Of these, 33 had dental amalgam restorations and 15 had no dental amalgam restorations. In those patients with amalgams, the total number of amalgam restorations and surfaces were counted, and the total and occlusal areas (mm(2)) of restorations were measured using a Counting Measurement Machine. Blood samples were collected from all participants. Plasma mercury levels were measured using an Atomic Absorption Spectrometer and Hydride System, and plasma TAA levels were measured using an Antioxidant Assay Kit. Statistical analysis was performed using the SPSS 10.01 software program. Data was evaluated by t test and correlation analysis. RESULTS: Plasma mercury (P-Hg) levels were found to be significantly higher in subjects with amalgam restorations when compared to subjects without amalgams (p<0.01); the differences in P-TAA levels between subjects with and without amalgams were not found to be statistically significant (p>0.05). No significant correlations were found between P-Hg concentrations and P-TAA levels (p>0.05). Significant positive correlations were found between P-Hg concentrations and the number of amalgam restorations (p<0.01), number of amalgam surfaces (p<0.05), total amalgam surface area (p<0.05) and amalgam occlusal surface area (p<0.01). However, no significant correlations were found between these parameters and P-TAA (p>0.05). CONCLUSIONS: The results of our study showed that dental amalgams are a major source of plasma mercury; however, amalgam restorations were not found to have a significant effect on plasma-total antioxidant activities.
Sujet(s)
Antioxydants/métabolisme , Amalgame dentaire/composition chimique , Mercure/sang , Adulte , Analyse de variance , Antioxydants/analyse , Restaurations dentaires permanentes , Femelle , Humains , Mâle , Spectrophotométrie atomique , Turquie , Jeune adulteRÉSUMÉ
This study was done to analyze the effect of different surfaces and different surface applications on the bonding strength of porcelain repair material and to compare these factors with one another. Three different substructures of 10 mm diameter and 4 mm thickness were used for the repair surface: metal, metal on porcelain and porcelain. The surfaces of half of the samples were roughened with an air abrasion tool; the surfaces of the other half were treated with a diamond bur. The specimens were ultrasonically cleaned in distilled water. A silane coupling agent and a bonding agent were applied to the surfaces of all the samples. Resin composite was applied to each specimen. All specimens were stored in distilled water for 24 hours before being thermocycled. After thermocycling, specimens were stored in distilled water for an additional seven days before being subjected to a shear load. The highest bonding strength was observed in the samples with a metal substructure, the surfaces of which were prepared with an air abrasion tool; the lowest bonding strength was observed in the samples with a metal substructure, the surfaces of which were prepared with a diamond bur. When different substructures were examined in terms of bonding strength, the highest bonding strength was statistically observed in metal-on-porcelain substructures, and the lowest bonding strength was observed in the porcelain substructure. When they were examined in terms of surface processes, the highest bonding strength was statistically observed in the samples whose surfaces were prepared with an air abrasion tool, and the lowest bonding strength was observed in the samples whose surfaces were prepared with a diamond bur.
Sujet(s)
Collage dentaire , Porcelaine dentaire , Réparation de prothèse dentaire , Céments résine , Air abrasion , Analyse de variance , Alliages de chrome , Analyse du stress dentaire , Test de matériaux , Alliages métal céramique , Résistance au cisaillement , Silanes , Propriétés de surfaceRÉSUMÉ
OBJECTIVE: To carry out measurements of the Hg levels and personal exposure in Turkish dental clinics, and to evaluate possible adverse effects on the CNS in dental personnel. SETTING: Five dental clinics (1 private, 4 public) in Erzurum, Turkey. SUBJECTS AND METHODS: 43, Hg vapour-exposed dental staff were examined and 43 hospital employees with no known exposure to Hg acted as the control group. Hg concentrations in plasma and urine were analysed by atomic absorption spectrophotometry. Possible effects on the central nervous system (CNS) were estimated by neuropsychological tests (Weschler Memory Scale-Revised (WMS-R) and Verbal Test of Memory Processes (VTMP)) and two self-administered questionnaires (Symptom Checklist-90-Revised (SCL-90-R) and Beck Depression Inventory (BDI)). RESULTS: The dental staff group had higher whole blood (B-Hg) and urine (U-Hg) Hg levels than the control group. The mean B-Hg value was 2.18 nmol/l and U-Hg was 1.17 nmol/mmol creatinine. U-Hg had an inverse relationship with logical memory (in WMS-R test) and total retention score (in VTMP test), and a positive relationship with increased scores of Anxiety and Psychoticism (in SCL-90-R). CONCLUSION: These results may represent long-term consequences of low Hg exposure. In dentistry, to decrease toxic effects, proper Hg hygiene should be practiced by all dental health care workers.
Sujet(s)
Personnel dentaire , Mercure/effets indésirables , Exposition professionnelle/effets indésirables , Adulte , Anxiété/induit chimiquement , Études cas-témoins , Dépression/induit chimiquement , Femelle , Humains , Mâle , Mémoire/effets des médicaments et des substances chimiques , Mercure/sang , Mercure/urine , Adulte d'âge moyen , Tests neuropsychologiques , Troubles psychotiques/étiologie , Analyse de régression , Spectrophotométrie atomique , Statistique non paramétrique , TurquieRÉSUMÉ
AIM: To determine whether there is a correlation between dental abrasions and the frequency and technique of tooth brushing, as well as to examine the prevalence of dental abrasion according to age and gender. MATERIALS AND METHODS: This study was carried out on 428 adults (242 female, 186 male) 20 years of age and older, who attended the Department of Operative Dentistry, Department of Oral Diagnosis and Oral Radiology Faculty of Dentistry, Atatürk University. RESULTS: The study revealed a statistically significant relationship between abrasive lesions and age groups as well as between abrasive lesions and gender. A statistically significant difference was determined between abrasions and tooth brushing frequency, while there was no statistically significant relation between abrasions and tooth brushing technique (p>0.05). CONCLUSION: The prevalence of tooth brushing abrasions increases with age, and lesions are seen more frequently in males than in females. Also, tooth brushing technique is not important to the presence of abrasions, but increased tooth brushing frequency results in an increase in the number of abrasions.