1.
Zh Mikrobiol Epidemiol Immunobiol
; (6): 86-8, 1998.
Article
de Russe
| MEDLINE
| ID: mdl-9949516
2.
Klin Lab Diagn
; (7): 25-6, 31, 1997 Jul.
Article
de Russe
| MEDLINE
| ID: mdl-9340088
RÉSUMÉ
Flow cytofluorometry of samples stained with fluorochrome-labeled antigen may be used to study the interactions between human blood calls and antigens. Using fluorescein isothiocyanate-labeled tuberculin (after the original method), the authors found that human blood monocytes actively bind labeled tuberculin. Studies of the concentrations from 0.1 to 12,500 ng/ml showed that saturation of the cell capacity for endocytosis is attained at certain doses, after which their surface label prevails. The share of tuberculin-binding lymphocytes was appreciably lower than that of monocytes at the same doses of the antigen. Clinical application of the method for assessing the antigen-presenting function of macrophages is discussed.