RÉSUMÉ
Many folding enzymes use separate domains for the binding of substrate proteins and for the catalysis of slow folding reactions such as prolyl isomerization. FKBP12 is a small prolyl isomerase without a chaperone domain. Its folding activity is low, but it could be increased by inserting the chaperone domain from the homolog SlyD of E. coli near the prolyl isomerase active site. We inserted two other chaperone domains into human FKBP12: the chaperone domain of SlpA from E. coli, and the chaperone domain of SlyD from Thermococcus sp. Both stabilized FKBP12 and greatly increased its folding activity. The insertion of these chaperone domains had no influence on the FKBP12 and the chaperone domain structure, as revealed by two crystal structures of the chimeric proteins. The relative domain orientations differ in the two crystal structures, presumably representing snapshots of a more open and a more closed conformation. Together with crystal structures from SlyD-like proteins, they suggest a path for how substrate proteins might be transferred from the chaperone domain to the prolyl isomerase domain.
Sujet(s)
Protéines Escherichia coli , Protéine 1A de liaison au tacrolimus , Humains , Escherichia coli/génétique , Chaperons moléculaires , Peptidylpropyl isomerase/génétique , CatalyseRÉSUMÉ
BACKGROUND: Recombinant interleukin-2 (IL-2, aldesleukin) is an approved cancer immunotherapy but causes severe toxicities including cytokine storm and vascular leak syndrome (VLS). IL-2 promotes antitumor function of IL-2Rß/γ+ natural killer (NK) cells and CD8+, CD4+ and gamma delta (γδ) T cells. However, IL-2 also potently activates immunosuppressive IL-2Rα+ regulatory T cells (Tregs) and IL-2Rα+ eosinophils and endothelial cells, which may promote VLS. Aldesleukin is rapidly cleared requiring frequent dosing, resulting in high Cmax likely potentiating toxicity. Thus, IL-2 cancer immunotherapy has two critical drawbacks: potent activation of undesired IL-2Rα+ cells and suboptimal pharmacokinetics with high Cmax and short half-life. METHODS: TransCon IL-2 ß/γ was designed to optimally address these drawbacks. To abolish IL-2Rα binding yet retain strong IL-2Rß/γ activity, IL-2 ß/γ was created by permanently attaching a small methoxy polyethylene glycol (mPEG) moiety in the IL-2Rα binding site. To improve pharmacokinetics, IL-2 ß/γ was transiently attached to a 40 kDa mPEG carrier via a TransCon (transient conjugation) linker creating a prodrug, TransCon IL-2 ß/γ, with sustained release of IL-2 ß/γ. IL-2 ß/γ was characterized in binding and primary cell assays while TransCon IL-2 ß/γ was studied in tumor-bearing mice and cynomolgus monkeys. RESULTS: IL-2 ß/γ demonstrated selective and potent human IL-2Rß/γ binding and activation without IL-2Rα interactions. TransCon IL-2 ß/γ showed slow-release pharmacokinetics with a low Cmax and a long (>30 hours) effective half-life for IL-2 ß/γ in monkeys. In mouse tumor models, TransCon IL-2 ß/γ promoted CD8+ T cell and NK cell activation and antitumor activity. In monkeys, TransCon IL-2 ß/γ induced robust activation and expansion of CD8+ T cells, NK cells and γδ T cells, relative to CD4+ T cells, Tregs and eosinophils, with no evidence of cytokine storm or VLS. Similarly, IL-2 ß/γ enhanced proliferation and cytotoxicity of primary human CD8+ T cells, NK cells and γδ T cells. SUMMARY: TransCon IL-2 ß/γ is a novel long-acting prodrug with sustained release of an IL-2Rß/γ-selective IL-2. It has remarkable and durable pharmacodynamic effects in monkeys and potential for improved clinical efficacy and tolerability compared with aldesleukin. TransCon IL-2 ß/γ is currently being evaluated in a Phase 1/2 clinical trial (NCT05081609).
Sujet(s)
Tumeurs , Promédicaments , Animaux , Lymphocytes T CD8+ , Syndrome de libération de cytokines , Préparations à action retardée/pharmacologie , Cellules endothéliales , Humains , Interleukine-2/pharmacologie , Sous-unité alpha du récepteur à l'interleukine-2 , Souris , Tumeurs/traitement médicamenteux , Promédicaments/pharmacologieRÉSUMÉ
Fine needle aspiration of peripheral lymph nodes is a minimal invasive procedure to extract material for cytological analyses. The method is safe, rapidly to apply, less expensive and almost painless for the patient. The puncture of low-lying lymph nodes shoud be performed via ultrasound navigation.
Sujet(s)
Cytoponction/méthodes , Cytoponction sous échoendoscopie/méthodes , Noeuds lymphatiques/imagerie diagnostique , Noeuds lymphatiques/anatomopathologie , Maladies lymphatiques/anatomopathologie , HumainsRÉSUMÉ
Integrins moderate diverse important functions in the human body and are promising targets in cancer therapy. Hence, the selective inhibition of specific integrins is of great medicinal interest. Here, we report the optimization of a grafted lasso peptide, yielding MccJ25(RGDF), which is a highly potent and selective αvß3 integrin inhibitor. Furthermore, its NMR structure was elucidated and employed in a molecular dynamics approach, revealing information about the integrin binding mode and selectivity profile of MccJ25(RGDF).
Sujet(s)
Intégrine alphaVbêta3/antagonistes et inhibiteurs , Peptides/composition chimique , Bactériocines/génétique , Adhérence cellulaire/effets des médicaments et des substances chimiques , Cellules endothéliales de la veine ombilicale humaine/effets des médicaments et des substances chimiques , Humains , Intégrine alphaVbêta3/composition chimique , Modèles moléculaires , Simulation de dynamique moléculaire , Résonance magnétique nucléaire biomoléculaire , Peptides/pharmacologie , Conformation des protéines , Venins de serpent/pharmacologieRÉSUMÉ
In this study we report the isolation, structure elucidation, and biosynthesis of mirubactin (1), a siderophore containing an unprecedented chemical functionality in natural products, namely, an O-acyl hydroxamic acid ester. Mirubactin represents the first siderophore isolated from the genus Actinosynnema and the first natural product produced by Actinosynnema mirum whose biosynthetic gene cluster could be identified. Structure elucidation was accomplished through a combination of spectroscopic (NMR, IR, and UV/vis) and mass spectrometric methods and revealed the presence of an unusual ester bond between the δ-N-hydroxyl group of δ-N-formyl-δ-N-hydroxyornithine and a 2,3-dihydroxybenzoate moiety. Bioinformatic analysis of the A. mirum genome and subsequent biochemical characterization of the putative biosynthetic machinery identified the gene cluster responsible for mirubactin assembly. The proposed biosynthesis of mirubactin comprises the iterative use of a stand-alone carrier-protein-bound substrate, as well as an ester-bond-forming step catalyzed by a C-terminal condensation domain, thus revealing an interesting system for further biochemical studies to gain a deeper understanding of nonribosomal peptide synthetase-catalyzed siderophore biosynthesis.
Sujet(s)
Actinomycetales/composition chimique , Acides hydroxamiques/isolement et purification , Sidérophores/isolement et purification , Actinomycetales/génétique , Esters , Acides hydroxamiques/composition chimique , Lactames/composition chimique , Structure moléculaire , Famille multigénique , Résonance magnétique nucléaire biomoléculaire , Sidérophores/biosynthèse , Sidérophores/composition chimique , Sidérophores/métabolismeRÉSUMÉ
Microcin J25 is the archetype of a growing class of bacterial ribosomal peptides possessing a knotted topology (lasso peptides). It consists of an eight-residue macrolactam ring through which the C-terminal tail is threaded. It is biosynthesized as a precursor that is processed by two maturation enzymes (McjB/McjC). Insights into the mechanism of microcin J25 biosynthesis have been provided previously by mutagenesis of the precursor peptide in vivo. In this study we have demonstrated distinct functions of McjB and McjC in vitro for the first time, based on the detection of reaction intermediates. McjB was characterized as a new ATP-dependent cysteine protease, whereas McjC was confirmed to be a lactam synthetase. The two enzymes were functionally interdependent, likely forming a structural complex. Their substrate preference was directly investigated with the aid of mutated precursor peptides. Depending on the substitutions, microcin J25 variants with either a lasso or branched-cyclic topology could be generated in vitro.
Sujet(s)
Bactériocines/composition chimique , Bactériocines/métabolisme , Séquence d'acides aminés , Antibactériens/composition chimique , Escherichia coli/composition chimique , Escherichia coli/enzymologie , Escherichia coli/métabolisme , Données de séquences moléculaires , Peptides/composition chimique , Peptides/métabolismeRÉSUMÉ
Subtilosin A is a 35-residue, ribosomally synthesized bacteriocin encoded by the sbo-alb operon of Bacillus subtilis. It is composed of a head-to-tail circular peptide backbone that is additionally restrained by three unusual thioether bonds between three cysteines and the α-carbon of one threonine and two phenylalanines, respectively. In this study, we demonstrate that these bonds are synthesized by the radical S-adenosylmethionine enzyme AlbA, which is encoded by the sbo-alb operon and comprises two [4Fe-4S] clusters. One [4Fe-4S] cluster is coordinated by the prototypical CXXXCXXC motif and is responsible for the observed S-adenosylmethionine cleavage reaction, whereas the second [4Fe-4S] cluster is required for the generation of all three thioether linkages. On the basis of the obtained results, we propose a new radical mechanism for thioether bond formation. In addition, we show that AlbA-directed substrate transformation is leader-peptide dependent, suggesting that thioether bond formation is the first step during subtilosin A maturation.
Sujet(s)
Bacillus subtilis/génétique , Protéines bactériennes/composition chimique , Protéines bactériennes/métabolisme , Bactériocines/métabolisme , Ferrosulfoprotéines/composition chimique , Ferrosulfoprotéines/métabolisme , Peptides cycliques/métabolisme , Sulfures/composition chimique , Motifs d'acides aminés , Séquence d'acides aminés , Bacillus subtilis/métabolisme , Protéines bactériennes/génétique , Bactériocines/composition chimique , Séquence nucléotidique , Sites de fixation , Cystéine/composition chimique , Spectroscopie de résonance de spin électronique , Ferrosulfoprotéines/génétique , Données de séquences moléculaires , Mutagenèse , Opéron , Peptides cycliques/composition chimique , Phénylalanine/composition chimique , Adémétionine/métabolisme , Thréonine/composition chimiqueRÉSUMÉ
Biosynthesis of the hydroxamate-type siderophore erythrochelin requires the generation of δ-N-acetyl-δ-N-hydroxy-L-ornithine (L-haOrn), which is incorporated into the tetrapeptide at positions 1 and 4. Bioinformatic analysis revealed the FAD-dependent monooxygenase EtcB and the bifunctional malonyl-CoA decarboxylase/acetyltransferase Mcd to be putatively involved in the generation of L-haOrn. To investigate if EtcB and Mcd constitute a two-enzyme pathway for the biosynthesis of L-haOrn, they were produced in a recombinant manner and subjected to biochemical studies in vitro. Hydroxylation assays employing recombinant EtcB gave rise to δ-N-hydroxy-L-ornithine (L-hOrn) and confirmed the enzyme to be involved in building block assembly. Acetylation assays were carried out by incubating L-hOrn with recombinant Mcd and malonyl-CoA as the acetyl group donor. Substrate turnover was increased by substituting malonyl-CoA with acetyl-CoA, bypassing the decarboxylation reaction which represents the rate-limiting step. Consecutive enzymatic synthesis of L-haOrn was accomplished in coupled assays employing both the L-ornithine hydroxylase and Mcd. In summary, a biosynthetic route for the generation of δ-N-acetyl-δ-N-hydroxy-L-ornithine starting from L-ornithine has been established in vitro by tandem action of the FAD-dependent monooxygenase EtcB and the bifunctional malonyl-CoA decarboxylase/acetyltransferase Mcd.
Sujet(s)
Acides hydroxamiques/métabolisme , Complexes multienzymatiques/biosynthèse , Oligopeptides/biosynthèse , Ornithine/analogues et dérivés , Saccharopolyspora/enzymologie , Sidérophores/biosynthèse , Acétylation , Acetyltransferases/composition chimique , Acetyltransferases/génétique , Carboxy-lyases/composition chimique , Carboxy-lyases/génétique , Carboxy-lyases/métabolisme , Décarboxylation , Pipérazinediones , Flavine adénine dinucléotide/composition chimique , Acides hydroxamiques/composition chimique , Hydroxylation , Complexes multienzymatiques/composition chimique , Complexes multienzymatiques/génétique , Famille multigénique , Oligopeptides/génétique , Ornithine/biosynthèse , Ornithine/génétique , Ornithine/métabolisme , Oxygénases/composition chimique , Saccharopolyspora/génétique , Sidérophores/génétique , Spécificité du substrat/génétiqueRÉSUMÉ
Lasso peptides constitute a class of bioactive peptides sharing a knotted structure where the C-terminal tail of the peptide is threaded through and trapped within an N-terminal macrolactam ring. The structural characterization of lasso structures and differentiation from their unthreaded topoisomers is not trivial and generally requires the use of complementary biochemical and spectroscopic methods. Here we investigated two antimicrobial peptides belonging to the class II lasso peptide family and their corresponding unthreaded topoisomers: microcin J25 (MccJ25), which is known to yield two-peptide product ions specific of the lasso structure under collision-induced dissociation (CID), and capistruin, for which CID does not permit to unambiguously assign the lasso structure. The two pairs of topoisomers were analyzed by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR MS) upon CID, infrared multiple photon dissociation (IRMPD), and electron capture dissociation (ECD). CID and ECD spectra clearly permitted to differentiate MccJ25 from its non-lasso topoisomer MccJ25-Icm, while for capistruin, only ECD was informative and showed different extent of hydrogen migration (formation of câ¢/z from c/zâ¢) for the threaded and unthreaded topoisomers. The ECD spectra of the triply-charged MccJ25 and MccJ25-lcm showed a series of radical b-type product ions (b'/â¢(n)). We proposed that these ions are specific of cyclic-branched peptides and result from a dual c/z⢠and y/b dissociation, in the ring and in the tail, respectively. This work shows the potentiality of ECD for structural characterization of peptide topoisomers, as well as the effect of conformation on hydrogen migration subsequent to electron capture.
Sujet(s)
Bactériocines/composition chimique , Spectrométrie de masse/méthodes , Peptides/composition chimique , Séquence d'acides aminés , Analyse de Fourier , Modèles moléculaires , Données de séquences moléculaires , Conformation des protéinesRÉSUMÉ
Capistruin, a ribosomally synthesized, post-translationally modified peptide produced by Burkholderia thailandensis E264, efficiently inhibits growth of Burkholderia and closely related Pseudomonas strains. The functional target of capistruin is not known. Capistruin is a threaded-lasso peptide (lariat peptide) consisting of an N-terminal ring of nine amino acids and a C-terminal tail of 10 amino acids threaded through the ring. The structure of capistruin is similar to that of microcin J25 (MccJ25), a threaded-lasso antibacterial peptide that is produced by some strains of Escherichia coli and targets DNA-dependent RNA polymerase (RNAP). Here, we show that capistruin, like MccJ25, inhibits wild type E. coli RNAP but not mutant, MccJ25-resistant, E. coli RNAP. We show further that an E. coli strain resistant to MccJ25, as a result of a mutation in an RNAP subunit gene, exhibits resistance to capistruin. The results indicate that the structural similarity of capistruin and MccJ25 reflects functional similarity and suggest that the functional target of capistruin, and possibly other threaded-lasso peptides, is bacterial RNAP.
Sujet(s)
Protéines bactériennes/antagonistes et inhibiteurs , Burkholderia/métabolisme , DNA-directed RNA polymerases/antagonistes et inhibiteurs , Antienzymes/métabolisme , Peptides/métabolisme , Séquence d'acides aminés , Escherichia coli/effets des médicaments et des substances chimiques , Données de séquences moléculaires , Conformation des protéinesRÉSUMÉ
The glucagon receptor antagonist BI-32169, recently isolated from Streptomyces sp., was described as a bicyclic peptide, although its primary structure comprises conserved elements of class I and class II lasso peptides. Tandem mass spectrometric and nuclear magnetic resonance spectroscopic studies revealed that BI-32169 is a lasso-structured peptide constituting the new class III of lasso peptides. The determined lasso fold opens new avenues to improve the promising biological activity of BI-32169.
Sujet(s)
Peptides cycliques/composition chimique , Peptides cycliques/pharmacologie , Récepteurs au glucagon/antagonistes et inhibiteurs , Séquence d'acides aminés , Protéines bactériennes/composition chimique , Protéines bactériennes/génétique , Protéines bactériennes/pharmacologie , Chromatographie en phase liquide à haute performance , Spectroscopie par résonance magnétique , Modèles moléculaires , Données de séquences moléculaires , Peptides cycliques/génétique , Conformation des protéines , Pliage des protéines , Streptomyces/métabolisme , Spectrométrie de masse en tandem/méthodesRÉSUMÉ
The class of nonribosomally assembled siderophores encompasses a multitude of structurally diverse natural products. The genome of the erythromycin-producing strain Saccharopolyspora erythraea contains 25 secondary metabolite gene clusters that are mostly considered to be orphan, including two that are responsible for siderophore assembly. In the present study, we report the isolation and structural elucidation of the hydroxamate-type tetrapeptide siderophore erythrochelin, the first nonribosomal peptide synthetase-derived natural product of S. erythraea. In an attempt to substitute the traditional activity assay-guided isolation of novel secondary metabolites, we have employed a dedicated radio-LC-MS methodology to identify nonribosomal peptides of cryptic gene clusters in the industrially relevant strain. This methodology was based on transcriptome data and adenylation domain specificity prediction and resulted in the detection of a radiolabeled ornithine-inheriting hydroxamate-type siderophore. The improvement of siderophore production enabled the elucidation of the overall structure via NMR and MS(n) analysis and hydrolysate-derivatization for the determination of the amino acid configuration. The sequence of the tetrapeptide siderophore erythrochelin was determined to be D-alpha-N-acetyl-delta-N-acetyl-delta-N-hydroxyornithine-D-serine-cyclo(L-delta-N-hydroxyornithine-L-delta-N-acetyl-delta-N-hydroxyornithine). The results derived from the structural and functional characterization of erythrochelin enabled the proposal of a biosynthetic pathway. In this model, the tetrapeptide is assembled by the nonribosomal peptide synthetase EtcD, involving unusual initiation- and cyclorelease-mechanisms.
Sujet(s)
Biosynthèse non ribosomique des peptides/génétique , Amino-acid ligases/métabolisme , Saccharopolyspora/génétique , Sidérophores/biosynthèse , Séquence d'acides aminés , Données de séquences moléculaires , Famille multigénique , Résonance magnétique nucléaire biomoléculaire , Oligopeptides/isolement et purification , Ornithine/analogues et dérivés , Ornithine/biosynthèse , Spectrométrie de masse en tandemSujet(s)
Anti-infectieux/métabolisme , Enzymes/métabolisme , Protéines Escherichia coli/métabolisme , Escherichia coli/enzymologie , Peptides/métabolisme , Pliage des protéines , Maturation post-traductionnelle des protéines , Séquence d'acides aminés , Anti-infectieux/composition chimique , Chromatographie en phase liquide à haute performance , Spectrométrie de masse , Données de séquences moléculaires , Peptides/composition chimiqueRÉSUMÉ
Capistruin is a 19-residue ribosomally synthesized lasso peptide encoded by the capABCD gene cluster in Burkholderia thailandensis. It is composed of an N-terminal 9-residue macrolactam ring, through which the 10-residue C-terminal tail is threaded. Using a heterologous capistruin production system in Escherichia coli, we have generated 48 mutants of the precursor protein CapA to gain insights into capistruin biosynthesis. Only 4 residues (Gly1, Arg11, Val12, and Ile13) of the lasso sequence were found to be critical for maturation. Tandem mass spectrometric fragmentation studies of capistruin F16A/F18A proved Arg15 to be responsible for the trapping of the C-terminal tail. Substituting Arg15 and Phe16 by alanine revealed a temperature-sensitive capistruin derivative, which unfolds into a branched cyclic peptide upon heating. In conclusion, our global mutagenic approach revealed a low overall specificity of the biosynthetic machinery and important structure-stability correlations.
Sujet(s)
Peptides/composition chimique , Séquence d'acides aminés , Burkholderia/composition chimique , Burkholderia/génétique , Données de séquences moléculaires , Famille multigénique , Mutation , Peptides/génétique , Peptides/métabolisme , Ingénierie des protéines , Stabilité protéique , Protéines recombinantes/biosynthèse , Protéines recombinantes/composition chimique , Protéines recombinantes/génétique , TempératureRÉSUMÉ
Lasso peptides are a structurally unique class of bioactive peptides characterized by a knotted arrangement, where the C-terminus threads through an N-terminal macrolactam ring. Although ribosomally synthesized, only the gene cluster for the best studied lasso peptide MccJ25 from Escherichia coli consisting of the precursor protein McjA and the processing and immunity proteins McjB, McjC, and McjD is known. Through genome mining studies, we have identified homologues of all four proteins in Burkholderia thailandensis E264 and predicted this strain to produce a lasso peptide. Here we report the successful isolation of the predicted peptide, named capistruin. Upon optimization of the fermentation conditions, mass spectrometric and NMR structural studies proved capistruin to adopt a novel lasso fold. Heterologous production of the lasso peptide in Escherichia coli showed that the identified genes are sufficient for the biosynthesis of capistruin, which exhibits antimicrobial activity against closely related Burkholderia and Pseudomonas strains. In general, our rational approach should be widely applicable for the isolation of new lasso peptides to explore their high structural stability and diverse biological activity.
Sujet(s)
Antibactériens/pharmacologie , Bactéries/effets des médicaments et des substances chimiques , Bactériocines/pharmacologie , Burkholderia pseudomallei , Génome bactérien , Peptides/pharmacologie , Séquence d'acides aminés , Antibactériens/composition chimique , Antibactériens/isolement et purification , Bactéries/croissance et développement , Bactériocines/composition chimique , Bactériocines/isolement et purification , Séquence nucléotidique , Burkholderia pseudomallei/composition chimique , Burkholderia pseudomallei/génétique , Escherichia coli/composition chimique , Escherichia coli/génétique , Fermentation , Spectroscopie par résonance magnétique , Spectrométrie de masse , Tests de sensibilité microbienne , Données de séquences moléculaires , Peptides/composition chimique , Peptides/isolement et purification , Réaction de polymérisation en chaîneRÉSUMÉ
The crystal structure of the bidomain PCP-C from modules 5 and 6 of the nonribosomal tyrocidine synthetase TycC was determined at 1.8 A resolution. The bidomain structure reveals a V-shaped condensation domain, the canyon-like active site groove of which is associated with the preceding peptidyl carrier protein (PCP) domain at its donor side. The relative arrangement of the PCP and the peptide bond-forming condensation (C) domain places the active sites approximately 50 A apart. Accordingly, this PCP-C structure represents a conformational state prior to peptide transfer from the donor-PCP to the acceptor-PCP domain, implying the existence of additional states of PCP-C domain interaction during catalysis. Additionally, PCP-C exerts a mode of cyclization activity that mimics peptide bond formation catalyzed by C domains. Based on mutational data and pK value analysis of active site residues, it is suggested that nonribosomal peptide bond formation depends on electrostatic interactions rather than on general acid/base catalysis.
Sujet(s)
Protéines bactériennes/composition chimique , Modèles moléculaires , Amino-acid ligases/composition chimique , Peptides/composition chimique , Séquence d'acides aminés , Sites de fixation , Cristallographie aux rayons X , Données de séquences moléculaires , Structure tertiaire des protéines , Spectrométrie de masse en tandemRÉSUMÉ
The catalytic activity of human FKBP12 as a prolyl isomerase is high towards short peptides, but very low in proline-limited protein folding reactions. In contrast, the SlyD proteins, which are members of the FKBP family, are highly active as folding enzymes. They contain an extra "insert-in-flap" or IF domain near the prolyl isomerase active site. The excision of this domain did not affect the prolyl isomerase activity of SlyD from Escherichia coli towards short peptide substrates but abolished its catalytic activity in proline-limited protein folding reactions. The reciprocal insertion of the IF domain of SlyD into human FKBP12 increased its folding activity 200-fold and generated a folding catalyst that is more active than SlyD itself. The IF domain binds to refolding protein chains and thus functions as a chaperone module. A prolyl isomerase catalytic site and a separate chaperone site with an adapted affinity for refolding protein chains are the key elements for a productive coupling between the catalysis of prolyl isomerization and conformational folding in the enzymatic mechanisms of SlyD and other prolyl isomerases, such as trigger factor and FkpA.
Sujet(s)
Protéines Escherichia coli/composition chimique , Chaperons moléculaires/composition chimique , Peptidylpropyl isomerase/composition chimique , Pliage des protéines , Structure tertiaire des protéines , Protéine 1A de liaison au tacrolimus/composition chimique , Protéine 1A de liaison au tacrolimus/métabolisme , Séquence d'acides aminés , Protéines Escherichia coli/génétique , Protéines Escherichia coli/métabolisme , Humains , Modèles moléculaires , Chaperons moléculaires/génétique , Chaperons moléculaires/métabolisme , Données de séquences moléculaires , Peptidylpropyl isomerase/génétique , Peptidylpropyl isomerase/métabolisme , Proline/métabolisme , Protéines de fusion recombinantes/composition chimique , Protéines de fusion recombinantes/génétique , Protéines de fusion recombinantes/métabolisme , Alignement de séquences , Protéine 1A de liaison au tacrolimus/génétiqueRÉSUMÉ
OBJECTIVE: Changes in body temperature occur as a systemic reaction to severe trauma; however, its role in the manifestation of injury remains unclear. Thermoregulatory responses vary considerably from fever to hypothermia. Although hypothermic trauma patients seem to have a worse prognosis, there is the question whether hypothermia per se or the severity of trauma producing the hypothermia is responsible for aggravated injury and increased mortality rate. The present study unravels how moderate to severe systemic hypothermia modulates local microcirculatory dysfunction and cellular injury in local soft tissue trauma. DESIGN: Prospective, experimental study. SETTING: Research laboratory at a university. SUBJECTS: C57BL/6J mice. INTERVENTIONS: A model involving standardized drop weight device-induced tissue trauma and high-resolution multifluorescence microscopy in the dorsal skinfold chamber was used to show arteriolar vasoconstriction, reduction of blood flow, nutritive perfusion failure, and apoptotic cell death at 1 hr after trauma. MEASUREMENTS AND MAIN RESULTS: During the 8-hr posttrauma observation period, microcirculation, but not apoptosis, restituted to almost baseline level. Concomitant systemic hypothermia of either 34 degrees C or 30 degrees C did not affect late manifestation of apoptotic cell death but aggravated initial microcirculatory dysfunction and inhibited recovery during the 8-hr follow-up period. CONCLUSIONS: Our study provides evidence that systemic hypothermia may aggravate soft tissue trauma-associated microcirculatory dysfunction. These experimental results clearly support clinical efforts to prevent hypothermia in the acutely traumatized patient.