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1.
Opt Express ; 32(3): 2972-2981, 2024 Jan 29.
Article de Anglais | MEDLINE | ID: mdl-38297532

RÉSUMÉ

Miniature free-space optical beams, originating from on-chip microstructures, are usually measured and quoted without reference to a particular polarization state. We develop an automated platform to characterize tightly focused free-space optical beams in three dimensions. We present a detailed description of each subsystem including the calibration and test procedure. We demonstrate how amplitude and phase are measured at sub-wavelength resolution using a cleaved fiber with a heterodyne reference. Further analysis provides information about the phase and intensity profile of the beam with regards to its polarization content and spatial confinement. We perform a proof-of-concept experiment for a custom waveguide-coupled micro-mirror. The work opens new possibilities for rapid analysis of micro-mirrors in prototyping and optimization of integrated optical systems.

2.
Ultramicroscopy ; 253: 113772, 2023 Nov.
Article de Anglais | MEDLINE | ID: mdl-37329810

RÉSUMÉ

This article provides a careful comparison between the electric and mechanical excitation of a tuning fork for shear force feedback in scanning probe microscopy, an analysis not found in present literature. A setup is designed and demonstrated for robust signal and noise measurements at comparable levels of physical movement of the probe. Two different signal amplification methods, combined with two excitation ways provide three possible configurations. For each method a quantitative analysis, supported by analytical elaboration and numerical simulations, is provided. Finally, it is shown that in practical circumstances electric excitation followed by detection with a transimpedance amplifier provides the best result.

3.
Rev Sci Instrum ; 82(6): 065111, 2011 Jun.
Article de Anglais | MEDLINE | ID: mdl-21721734

RÉSUMÉ

This article demonstrates a quick and easy way of quantifying the performance of a vibration-isolated platform. We measure the vibration transfer from floor to table using background noise excitation from the floor. As no excitation device is needed, our setup only requires two identical sensors (in our case, low noise accelerometers), a data acquisition system, and processing software. Background noise excitation from the floor has the additional advantage that any non-linearity in the suspension system relevant to the actual vibration amplitudes will be taken into account. Measurement time is typically a few minutes, depending on the amount of background noise. The (coherent) transfer of the vibrations in the floor to the platform, as well as the (non-coherent) acoustical noise pick-up by the platform are measured. Since we use calibrated sensors, the absolute value of the vibration levels is established and can be expressed in vibration criterion curves. Transfer measurements are shown and discussed for two pneumatic isolated optical tables, a spring suspension system, and a simple foam suspension system.

4.
Phys Rev Lett ; 103(4): 043905, 2009 Jul 24.
Article de Anglais | MEDLINE | ID: mdl-19659356

RÉSUMÉ

In biological samples the resonant coherent anti-Stokes Raman scattering signal of less abundant constituents can be overwhelmed by the nonresonant background, preventing detection of those molecules. We demonstrate a method to obtain the phase of the oscillators in the focal volume that allows discrimination of those hidden molecules. The phase is measured with respect to the local excitation fields using a cascaded phase-preserving chain. It is measured point-by-point and takes into account refractive index changes in the sample, phase curvature over the field-of-view, and interferometric instabilities. The detection of the phase of the vibrational motion can be regarded as a vibrational extension of the linear (refractive index) phase contrast microscopy introduced by Zernike around 1933.


Sujet(s)
Microscopie de contraste de phase/méthodes , Analyse spectrale Raman/méthodes , Cellules HeLa , Humains , Interférométrie/méthodes
5.
J Microsc ; 233(2): 234-43, 2009 Feb.
Article de Anglais | MEDLINE | ID: mdl-19220689

RÉSUMÉ

We present a novel atomic force microscope (AFM) system, operational in liquid at variable gravity, dedicated to image cell shape changes of cells in vitro under hypergravity conditions. The hypergravity AFM is realized by mounting a stand-alone AFM into a large-diameter centrifuge. The balance between mechanical forces, both intra- and extracellular, determines both cell shape and integrity. Gravity seems to be an insignificant force at the level of a single cell, in contrast to the effect of gravity on a complete (multicellular) organism, where for instance bones and muscles are highly unloaded under near weightless (microgravity) conditions. However, past space flights and ground based cell biological studies, under both hypogravity and hypergravity conditions have shown changes in cell behaviour (signal transduction), cell architecture (cytoskeleton) and proliferation. Thus the role of direct or indirect gravity effects at the level of cells has remained unclear. Here we aim to address the role of gravity on cell shape. We concentrate on the validation of the novel AFM for use under hypergravity conditions. We find indications that a single cell exposed to 2 to 3 x g reduces some 30-50% in average height, as monitored with AFM. Indeed, in situ measurements of the effects of changing gravitational load on cell shape are well feasible by means of AFM in liquid. The combination provides a promising technique to measure, online, the temporal characteristics of the cellular mechano-response during exposure to inertial forces.


Sujet(s)
Forme de la cellule , Surpesanteur , Microscopie à force atomique/instrumentation , Microscopie à force atomique/méthodes , Ostéoblastes/cytologie , Animaux , Substances tampon , Cellules cultivées , Centrifugation , Milieux de culture , Électronique , Souris , Ostéoblastes/ultrastructure , Logiciel , Substances viscoélastiques
6.
Opt Express ; 16(20): 15863-9, 2008 Sep 29.
Article de Anglais | MEDLINE | ID: mdl-18825222

RÉSUMÉ

In this article we show that heterodyne CARS, based on a controlled and stable phase-preserving chain, can be used to measure amplitude and phase information of molecular vibration modes. The technique is validated by a comparison of the imaginary part of the heterodyne CARS spectrum to the spontaneous Raman spectrum of polyethylene. The detection of the phase allows for rejection of the non-resonant background from the data. The resulting improvement of the signal to noise ratio is shown by measurements on a sample containing lipid.


Sujet(s)
Lipides/composition chimique , Optique et photonique , Analyse spectrale Raman/instrumentation , Analyse spectrale Raman/méthodes , Algorithmes , Conception d'appareillage , Interférométrie/instrumentation , Interférométrie/méthodes , Modèles statistiques , Diffusion de rayonnements
7.
Opt Express ; 16(11): 7985-96, 2008 May 26.
Article de Anglais | MEDLINE | ID: mdl-18545508

RÉSUMÉ

By spectral phase shaping of both the pump and probe pulses in coherent anti-Stokes Raman scattering (CARS) spectroscopy we demonstrate the extraction of the frequencies, bandwidths and relative cross sections of vibrational lines. We employ a tunable broadband Ti:Sapphire laser synchronized to a ps-Nd:YVO mode locked laser. A high resolution spectral phase shaper allows for spectroscopy with a precision better than 1 cm(-1) in the high frequency region around 3000 cm(-1). We also demonstrate how new spectral phase shaping strategies can amplify the resonant features of isolated vibrations to such an extent that spectroscopy and microscopy can be done at high resolution, on the integrated spectral response without the need for a spectrograph.


Sujet(s)
Conception assistée par ordinateur , Lasers , Modèles théoriques , Optique et photonique/instrumentation , Réfractométrie/instrumentation , Analyse spectrale Raman/instrumentation , Simulation numérique , Conception d'appareillage , Analyse de panne d'appareillage , Analyse spectrale Raman/méthodes
8.
Opt Express ; 16(3): 1958-64, 2008 Feb 04.
Article de Anglais | MEDLINE | ID: mdl-18542275

RÉSUMÉ

We report the near-field observation of the phase shifts associated with total internal reflection on a glass-air interface and surface plasmon resonance on a glass-gold-air system. The phase of the evanescent waves on glass and gold surfaces, as a function of incident angle, is measured using a phase-sensitive Photon Scanning Tunneling Microscope (PSTM) and shows a good agreement with theory.


Sujet(s)
Algorithmes , Amélioration d'image/méthodes , Interprétation d'images assistée par ordinateur/méthodes , Microscopie à effet tunnel/méthodes , Réfractométrie/méthodes , Résonance plasmonique de surface/méthodes
9.
Rev Sci Instrum ; 79(1): 013704, 2008 Jan.
Article de Anglais | MEDLINE | ID: mdl-18248036

RÉSUMÉ

We describe the realization of a phase-sensitive and ultrafast near-field microscope, optimized for investigation of surface plasmon polariton propagation. The apparatus consists of a homebuilt near-field microscope that is incorporated in Mach-Zehnder-type interferometer which enables heterodyne detection. We show that this microscope is able to measure dynamical properties of both photonic and plasmonic systems with phase sensitivity.


Sujet(s)
Interférométrie/instrumentation , Microscopie à sonde à balayage/instrumentation , Résonance plasmonique de surface/instrumentation , Conception d'appareillage , Analyse de panne d'appareillage , Interférométrie/méthodes , Microscopie à sonde à balayage/méthodes , Reproductibilité des résultats , Sensibilité et spécificité , Résonance plasmonique de surface/méthodes
10.
Opt Express ; 15(23): 15207-13, 2007 Nov 12.
Article de Anglais | MEDLINE | ID: mdl-19550804

RÉSUMÉ

We demonstrate heterodyne detection of CARS signals using a cascaded phase-preserving chain to generate the CARS input wavelengths and a coherent local oscillator. The heterodyne amplification by the local oscillator reveals a window for shot noise limited detection before the signal-to-noise is limited by amplitude fluctuations. We demonstrate an improvement in sensitivity by more than 3 orders of magnitude for detection using a photodiode. This will enable CARS microscopy to reveal concentrations below the current mMolar range.

11.
Opt Express ; 14(4): 1658-72, 2006 Feb 20.
Article de Anglais | MEDLINE | ID: mdl-19503493

RÉSUMÉ

We have studied the dispersion of ultrafast pulses in a photonic crystal waveguide as a function of optical frequency, in both experiment and theory. With phase-sensitive and time-resolved near-field microscopy, the light was probed inside the waveguide in a non-invasive manner. The effect of dispersion on the shape of the pulses was determined. As the optical frequency decreased, the group velocity decreased. Simultaneously, the measured pulses were broadened during propagation, due to an increase in group velocity dispersion. On top of that, the pulses exhibited a strong asymmetric distortion as the propagation distance increased. The asymmetry increased as the group velocity decreased. The asymmetry of the pulses is caused by a strong increase of higher order dispersion. As the group velocity was reduced to 0.116(9) .c, we found group velocity dispersion of -1.1(3) .10(6) ps(2)/km and third order dispersion of up to 1.1(4) .10(5) ps(3)/km. We have modelled our interferometric measurements and included the full dispersion of the photonic crystal waveguide. Our mathematical model and the experimental findings showed a good correspondence. Our findings show that if the most commonly used slow light regime in photonic crystals is to be exploited, great care has to be taken about higher-order dispersion.

12.
Nano Lett ; 5(11): 2144-8, 2005 Nov.
Article de Anglais | MEDLINE | ID: mdl-16277442

RÉSUMÉ

We report on the concept, generation, and first observations of focused surface plasmons on shaped gratings. The grating patterns are designed to realize focusing and directing through noncollinear phasematching. The plasmons are generated on patterned gold surfaces, and the plasmon propagation is observed using phase-sensitive photon scanning tunneling microscopy (PSTM) to extract the propagation pattern, direction, and wavelength.

13.
Phys Rev Lett ; 94(12): 123901, 2005 Apr 01.
Article de Anglais | MEDLINE | ID: mdl-15903920

RÉSUMÉ

The eigenfield distribution and the band structure of a photonic crystal waveguide have been measured with a phase-sensitive near-field scanning optical microscope. Bloch modes, which consist of more than one spatial frequency, are visualized in the waveguide. In the band structure, multiple Brillouin zones due to zone folding are observed, in which positive and negative dispersion is seen. The negative slopes are shown to correspond to a negative phase velocity but a positive group velocity. The lateral mode profile for modes separated by one reciprocal lattice vector is found to be different.

14.
Phys Rev Lett ; 94(7): 073903, 2005 Feb 25.
Article de Anglais | MEDLINE | ID: mdl-15783818

RÉSUMÉ

We show the real-space observation of fast and slow pulses propagating inside a photonic crystal waveguide by time-resolved near-field scanning optical microscopy. Local phase and group velocities of modes are measured. For a specific optical frequency we observe a localized pattern associated with a flat band in the dispersion diagram. During at least 3 ps, movement of this field is hardly discernible: its group velocity would be at most c/1000. The huge trapping times without the use of a cavity reveal new perspectives for dispersion and time control within photonic crystals.

15.
Opt Lett ; 29(11): 1291-3, 2004 Jun 01.
Article de Anglais | MEDLINE | ID: mdl-15209275

RÉSUMÉ

A noninvasive pulse-tracking technique has been exploited to observe the time-resolved motion of an ultrashort light pulse within an integrated optical microresonator. We follow a pulse as it completes several round trips in the resonator, directly mapping the resonator modes in space and time. Our time-dependent and phase-sensitive measurement provides direct access to the angular group and phase velocity of the modes in the resonator. From the measurement the coupling constants between the access waveguides and the resonator are retrieved while at the same time the loss mechanisms throughout the structure are directly visualized.

16.
Phys Rev E Stat Nonlin Soft Matter Phys ; 70(6 Pt 2): 066609, 2004 Dec.
Article de Anglais | MEDLINE | ID: mdl-15697528

RÉSUMÉ

The amplitude and phase evolution of ultrashort pulses in a bimodal waveguide structure has been studied with a time-resolved photon scanning tunneling microscope (PSTM). When waveguide modes overlap in time intriguing phase patterns are observed. Phase singularities, arising from interference between different modes, are normally expected at equidistant intervals determined by the difference in effective index for the two modes. However, in the pulsed experiments the distance between individual singularities is found to change not only within one measurement frame, but even depends strongly on the reference time. To understand this observation it is necessary to take into account that the actual pulses generating the interference signal change shape upon propagation through a dispersive medium. This implies that the spatial distribution of phase singularities contains direct information on local dispersion characteristics. At the same time also the mode profiles, wave vectors, pulse lengths, and group velocities of all excited modes in the waveguide are directly measured. The combination of these parameters with an analytical model for the time-resolved PSTM measurements shows that the unique spatial phase information indeed gives a direct measure for the group velocity dispersion of individual modes. As a result interesting and useful effects, such as pulse compression, pulse spreading, and pulse reshaping become accessible in a local measurement.

17.
Phys Rev E Stat Nonlin Soft Matter Phys ; 68(2 Pt 2): 026604, 2003 Aug.
Article de Anglais | MEDLINE | ID: mdl-14525130

RÉSUMÉ

We report on the direct visualization of a femtosecond pulse propagating through a dispersive waveguide at a telecom wavelength. The position of a propagating pulse is pinpointed at a particular point in space and time using a scanning probe based measurement. The actual propagation of the pulse is visualized by changing the reference time. Our phase-sensitive and time-resolved measurement provides local information on all properties of the light pulse as it propagates, in particular its phase and group velocity. Here, we show that the group velocity dispersion can be retrieved from our measurement by developing an analytical model for the measurements performed with a time-resolved photon scanning tunneling microscope. As a result, interesting and useful effects, such as pulse compression, pulse spreading, and pulse reshaping, become accessible in the local measurement.

18.
Science ; 294(5544): 1080-2, 2001 Nov 02.
Article de Anglais | MEDLINE | ID: mdl-11691986

RÉSUMÉ

We show that the propagation of a femtosecond laser pulse inside a photonic structure can be directly visualized and tracked as it propagates using a time-resolved photon scanning tunneling microscope. From the time-dependent and phase-sensitive measurements, both the group velocity and the phase velocity are unambiguously and simultaneously determined. It is expected that this technique will find applications in the investigation of the local dynamic behavior of photonic crystals and integrated optical circuits.

19.
J Microsc ; 202(Pt 1): 104-9, 2001 Apr.
Article de Anglais | MEDLINE | ID: mdl-11298878

RÉSUMÉ

For the first time the local optical phase evolution in and around a small, one-dimensional photonic crystal has been visualized with a heterodyne interferometric photon scanning tunnelling microscope. The measurements show an exponential decay of the optical intensity inside the crystal, which consists of a periodic array of subwavelength air rods fabricated in a conventional ridge waveguide. In addition it is found that the introduction of the air rods has a counterintuitive effect on the phase development inside the structure. The heterodyne detection scheme allows the detection of low-intensity scattered waves. In the vicinity of the scattering air rods phase singularities are found with a topological charge of plus or minus one.

20.
Opt Lett ; 25(9): 637-9, 2000 May 01.
Article de Anglais | MEDLINE | ID: mdl-18064135

RÉSUMÉ

The simultaneous detection of TE- as well as TM-polarized light with a photon scanning tunneling microscope leads to a quasi-interference pattern of these mutually perpendicular polarized fields. This interference pattern has been observed in the optical field distribution as a function of both position and wavelength. Comparison of experimental data with simulations confirms the interference of mutually orthogonal fields. This quasi interference is caused by conversion of the linearly polarized light of both modes into elliptically polarized light by a fiber probe.

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