Plasma-grating-based laser pulse compressor.

To avoid damage in high-power laser systems, a chirped plasma-based grating is proposed for compressing laser pulses that have been previously stretched and amplified. This chirped grating is generated through the interaction of chirped pump laser pulses in a plasma slab. Particle-in-cell (PIC) simulations demonstrate that the grating exists for a duration sufficient to be utilized in the final chirped pulse amplification (CPA) stage. The generation of the grating is quite flexible, as several parameters can be adjusted, such as plasma density, chirp, length, and intensity of the pump laser. To begin, the structure of the grating is analyzed in terms of ponderomotive effects of the pump laser pulses. The primary application of the chirped plasma-based grating lies in compressing laser pulses to large amplitudes and short durations after they have been stretched and amplified beforehand. The compression factor is explored in connection with potential grating parameters. Reflectivity and effective bandwidth of chirped plasma gratings are parameters to be optimized. However, the grating spectral bandwidth can only be increased at the expense of reflectivity. The PIC results are made understandable through model calculations based on coupled mode equations.

Formation and properties of spatially inhomogeneous plasma density gratings.

Volume plasma density gratings receive increasing interest since, compared to solid-state optical media, they posses significantly higher damage thresholds. The gratings are produced by counterpropagating laser pulses in underdense plasma. When analyzing their optical properties, usually they are assumed to be homogeneous in space. The latter assumption, however, breaks down, especially when the gratings are produced by short high-power laser pump pulses. Then, generically the plasma grating posses an inhomogeneous envelope which results from the superposition of the pump pulses envelopes. The present paper discusses the effect of grating inhomogeneity on reflection and transmission of probe pulses. A Gaussian plasma density grating becomes an apodized grating which offers significant improvement over homogeneous gratings due to side-lobe suppression while maintaining reflectivity and a narrow bandwidth. On the other hand, the reflected probe pulses receive a chirp which depends on the spatial scale. For a Gaussian grating a cubic spectral phase appears. Numerical particle-in-cell simulations are supported by theoretical analysis based on coupled mode equations as well as an effective medium approach.

[Recurrent choroidal detachment in a patient undergoing hormone therapy for prostate cancer]. / Rezidivierende Aderhautamotio bei einem Patienten mit hormonell therapiertem Prostatakarzinom.

An 85-year-old male patient receiving hormone therapy for prostate cancer and secondary open-angle pseudoexfoliation glaucoma developed peripheral choroidal detachment in both eyes. The patient had been admitted to the eye hospital for clarification of a vascular occlusion in the left eye. Cerebral magnetic resonance imaging could exclude metastases and a cerebral space-occupying lesion as the cause of the ocular findings. The local antiglaucomatous treatment was interrupted and as a result the intraocular pressure normalized and the choroidal detachment receded completely. Patients with prostate cancer who receive hormone therapy should also undergo regular ophthalmological screening including funduscopy and measurment of intraocular pressure.

Plasma volume holograms for focusing and mode conversion of ultraintense laser pulses.

Beating of a broad laser reference beam with a quite general focused object beam inside a plasma volume generates a dynamic plasma hologram. Both beams may be of moderate intensity. The volume hologram can be read out by an ultraintense main beam (of similar structure as the reference beam) producing an object beam replica. For the latter, intensity in the focus may become extremely large. As an application, the possibility of a read-out focused Gaussian laser pulse with intensity of several 10^{19}W/cm^{2} in focus is shown by three-dimensional numerical simulations. Besides the focusing possibility, the hologram may also act as a mode converter for high-intensity laser pulses. Generating a plasma hologram with a focused Laguerre-Gaussian object beam results in a staggered plasma density grating, allowing the production of high-intensity vortex beam replica.

Plasma-based polarizer and waveplate at large laser intensity.

A plasma photonic crystal consists of a plasma density grating which is created in underdense plasma by counterpropagating laser beams. When a high-power laser pulse impinges the crystal, it might be reflected or transmitted. So far only one type of pulse polarization, namely the so-called s wave (or TE mode) was investigated (when the electric field vector is perpendicular to the plane of incidence). Here, when investigating also so-called p waves (or TM modes, where the magnetic field vector is perpendicular to the plane of incidence), it is detected that the transmission and reflection properties of the plasma photonic crystal depend on polarization. A simple analytic model of the crystal allows one to make precise predictions. The first conclusion is that in some operational regime the crystal can act as a plasma polarizer for high-intensity laser pulses. Also, differences in phase velocities for grazing incidence between s and p polarization are found. Thus, secondly, the crystal can be utilized as a waveplate, e.g., transforming linearly polarized laser light into circular polarization. All these processes extend to laser intensities beyond the damage intensities of so far used solid state devices.

Parametric pulse amplification by acoustic quasimodes in electron-positron plasma.

In a recent paper, M. R. Edwards, N. J. Fisch, and J. M. Mikhailova [Phys. Rev. Lett. 116, 015004 (2016)PRLTAO0031-900710.1103/PhysRevLett.116.015004] reported that in electron-positron plasma stimulated Brillouin scattering is drastically enhanced, while stimulated Raman scattering is completely absent. However, when theory was compared to particle-in-cell (PIC) simulations, a discrepancy by at least a factor four appeared. Authors correctly argued that the disparity might be due to the fluid approximation of the low-frequency mode. They noted that a more precise analytic description of the acoustic resonance requires a kinetic approach, which was beyond the scope of the mentioned paper. Here we deliver the so-far-missing kinetic calculation. It shows quite good agreement with the PIC simulations presented in the above-mentioned paper by Edwards et al. The principal result of enhancement of Brillouin scattering and absence of Raman scattering remains valid. The Brillouin enhancement factors depend on electron temperature and background particle density. These dependencies as well as the transition to the well-known behavior of electron-ion plasma are discussed. It is also shown that pulse amplification in electron-positron plasma crosses over to the strong-coupling regime when the pump amplitude becomes large. Then, the fluid approximation becomes acceptable again.

DNA barcoding of crickets, katydids and grasshoppers (Orthoptera) from Central Europe with focus on Austria, Germany and Switzerland.

We present a DNA barcoding study on the insect order Orthoptera that was generated in collaboration between four barcoding projects in three countries, viz. Barcoding Fauna Bavarica (Germany), German Barcode of Life, Austrian Barcode of Life and Swiss Barcode of Life. Our data set includes 748 COI sequences from 127 of the 162 taxa (78.4%) recorded in the three countries involved. Ninety-three of these 122 species (76.2%, including all Ensifera) can be reliably identified using DNA barcodes. The remaining 26 caeliferan species (families Acrididae and Tetrigidae) form ten clusters that share barcodes among up to five species, in three cases even across different genera, and in six cases even sharing individual barcodes. We discuss incomplete lineage sorting and hybridization as most likely causes of this phenomenon, as the species concerned are phylogenetically young and hybridization has been previously observed. We also highlight the problem of nuclear mitochondrial pseudogenes (numts), a known problem in the barcoding of orthopteran species, and the possibility of Wolbachia infections. Finally, we discuss the possible taxonomic implications of our barcoding results and point out future research directions.

Transient Plasma Photonic Crystals for High-Power Lasers.

A new type of transient photonic crystals for high-power lasers is presented. The crystal is produced by counterpropagating laser beams in plasma. Trapped electrons and electrically forced ions generate a strong density grating. The lifetime of the transient photonic crystal is determined by the ballistic motion of ions. The robustness of the photonic crystal allows one to manipulate high-intensity laser pulses. The scheme of the crystal is analyzed here by 1D Vlasov simulations. Reflection or transmission of high-power laser pulses are predicted by particle-in-cell simulations. It is shown that a transient plasma photonic crystal may act as a tunable mirror for intense laser pulses. Generalizations to 2D and 3D configurations are possible.

Adenoviral transfer of human aquaporin-1 gene to rat liver improves bile flow in estrogen-induced cholestasis.

Estrogens can cause liver cholestatic disease. As downregulation of hepatocyte canalicular aquaporin-8 (AQP8) water channels has been involved in estrogen-induced bile secretory failure, we tested whether the archetypal water channel AQP1 improves 17α-ethinylestradiol (EE)-induced cholestasis. EE administration to rats reduced bile flow by 50%. A recombinant adenoviral (Ad) vector encoding human AQP1 (hAQP1), AdhAQP1, or a control vector was administered by retrograde bile ductal infusion. Hepatocyte canalicular hAQP1 expression was confirmed by liver immunostaining and immunoblotting in purified membrane fractions. Accordingly, canalicular osmotic water permeability was markedly increased. Bile flow, either basal or bile salt-stimulated was significantly augmented by over 50%. The choleretic efficiency of endogenous bile salts (that is, volume of bile per µmol of excreted bile salt) was significantly increased by 45% without changes in the biliary bile salt composition. Our data suggest that the adenoviral transfer of hAQP1 gene to the livers of EE-induced cholestatic rats improves bile flow by enhancing the AQP-mediated bile salt-induced canalicular water secretion. This novel finding might have potential therapeutic implications for cholestatic diseases.

[Pharmaceutical therapy of bone metabolism disorders in chronic kidney disease mineral bone disorder (CKD-MBD) with special respect to antiresorptive substances]. / Medikamentöse Therapie von Knochenstoffwechselstörungen bei CKD-MBD ("chronic kidney disease - mineral bone disorder") unter besonderer Berücksichtigung antiresorptiver Wirkstoffe.

Disturbances in bone and mineral turnover are common complications in patients with impaired renal function. Besides an increased risk for cardiovascular events they promote skeletal events, such as bone pain and fractures. Evidence for the antifracture efficacy of antiresorptive and osteoanabolic treatment strategies has only been demonstrated for patients with osteoporosis. The use of osteotropic drugs in patients with impaired renal function requires large randomized placebo-controlled trials.

Sensory evolution of hearing in tettigoniids with differing communication systems.

In Tettigoniidae (Orthoptera: Ensifera), hearing organs are essential in mate detection. Male tettigoniids usually produce calling songs by tegminal stridulation, whereas females approach the males phonotactically. This unidirectional communication system is the most common one among tettigoniids. In several tettigoniid lineages, females have evolved acoustic replies to the male calling song which constitutes a bidirectional communication system. The genus Poecilimon (Tettigoniidae: Phaneropterinae) is of special interest because the ancestral state of bidirectional communication, with calling males and responding females, has been reversed repeatedly to unidirectional communication. Acoustic communication is mediated by hearing organs that are adapted to the conspecific signals. Therefore, we analyse the auditory system in the Tettigoniidae genus Poecilimon for functional adaptations in three characteristics: (i) dimension of sound-receiving structures (tympanum and acoustic spiracle), (ii) number of auditory sensilla and (iii) hearing sensitivity. Profound differences in the auditory system correlate with uni- or bidirectional communication. Among the sound-receiving structures, the tympana scale with body size, whereas the acoustic spiracle, the major sound input structure, was drastically reduced in unidirectional communicating species. In the unidirectional P. ampliatus group, auditory sensilla are severely reduced in numbers, but not in the unidirectional P. propinquus group. Within the P. ampliatus group, the number of auditory sensilla is further reduced in P. intermedius which lost acoustic signalling due to parthenogenesis. The auditory sensitivity correlated with the size of the acoustic spiracle, as hearing sensitivity was better with larger spiracles, especially in the ultrasonic range. Our results show a significant reduction in auditory structures, shaped by the differing sex roles during mate detection.

Molecular and classical chromosomal techniques reveal diversity in bushcricket genera of Barbitistini (Orthoptera).

The cytogenetic characteristics of 17 species of bushcricket belonging to eight genera of the tribe Barbitistini were examined by fluorescence in situ hybridization with 18S rDNA and (TTAGGn) telomeric as probes and by C-banding, silver, and fluorochrome staining. These markers were used to understand chromosomal organization and evolutionary relationships between genera or species within the same genus. The number of 18S rDNA clusters per haploid genome that co-localized with active nucleolus organizer regions (NORs) ranged from one to five, with the most common pattern being the presence of one NOR-bearing chromosome. This ribosomal cistron was preferentially located in the paracentromeric region of autosomes and very rarely in the sex chromosome. The results demonstrated coincidence between the localization of major ribosomal genes and active NORs and the position of C-band and GC-rich regions. The rDNA/NOR distribution and the composition of chromosome heterochromatin proved to be good cytogenetic markers for distinguishing species and phylogenetic lines and for understanding the genomic differentiation and evolution of Barbitistini. A comparison of cytogenetic and morphological or behavioral traits suggests that morphological and behavioral specialization in this group was not followed by major karyotype modification (except for Leptophyes). However, the occurrence and distribution of different repetitive DNA sites tends to vary among the taxa.

Pulse shaping during Raman-seed amplification for short laser pulses.

Raman-seed pulse amplification in a one-dimensional backscattering geometry is investigated with the help of numerical simulations and analytical estimates. The significant dependence of the initial amplification on the pulse form is revisited on the basis of a three-wave interaction as well as a kinetic Vlasov model. It is shown how the short duration of the input seed pulse influences its subsequent behavior, depending on plasma density and pump strength. The evolution during a "start-up period," which has been observed earlier, can be explained analytically. In the nonlinear (pump depletion) regime, the pulse generated in the start-up period will be further amplified and may evolve into a self-similar π-pulse solution. The Vlasov code predicts algebraic growth in time of the seed amplitude, similar to the findings based on self-similar solutions of the three-wave-interaction model. An initially very narrow pulse is shown to grow more slowly than an initially broad one.

[Indications and prerequisites for bone biopsy]. / Indikationen und Voraussetzungen zur Knochenbiopsie.

The iliac crest biopsy provides additional information for the therapeutic approach in selected questions if the indication is correct. Indications include osteoporosis with an atypical course with respect to age, fracture frequency or success of therapy. The aim of the iliac crest biopsy is to harvest an undestroyed and intact piece of bone, containing all compartments, including cancellous bone, cortical bone and bone marrow. Knowledge of clinical and paraclinical parameters is essential for diagnosis and assessment. A safe sampling technique under sterile conditions must be guaranteed. The biopsy itself takes place in the anterior superior iliac spine and posterior superior iliac spine, preferably by using a Burkhardt or a diamond burr. Hard cutting technique and knowledge or the histomorphometry are indispensable prerequisites.

Phase-space contraction and attractors for ultrarelativistic electrons.

Ultrashort-pulse laser intensities can reach 10(22) W/cm(2). In this case the electron motion becomes ultrarelativistic and significant bremsstrahlung occurs. The radiation causes a dissipative effect, which is called a radiation reaction. It has been shown in the literature that the radiation reaction force causes phase-space contraction when the motion of electrons in a laser field is considered. The effect of the radiation reaction force is smaller for electron propagation in the direction of a propagating plane wave compared to counterpropagation. In the case of two colliding laser beams with sufficiently large amplitudes, stochastic heating is an important process that will be influenced by the radiation reaction. It is shown here that the radiation reaction causes attractors and in certain parameter regimes electron motion converges to regular attractors. This causes a significant reduction of stochastic heating. The forms of the attractors are presented. The results confirm the general prediction of phase-space contraction and provide quantitative results for the speed of contraction as well as the type of attracted motion.

Monthly ibandronate for the prevention of bone loss in patients after liver transplantation.

BACKGROUND: Osteopenia and osteoporosis are diseases frequently occurring after liver transplantation (OLT). PURPOSE: In a prospective study, we have investigated the effect of ibandronate, vitamin D(3), and calcium on the prevention and treatment of posttransplant osteopenia and osteoporosis. METHODS: The bone mineral density (BMD) of the lumbar spine (LS) and of the femoral neck (FN) were measured in 74 patients prospectively pre- and post-OLT. RESULTS: Postoperatively the study group showed a consistent percentage increase in BMD (g/cm(2)) and a significantly increased BMD after 12 and 24 months in the LS (12 months: 1.05 ± 0.21 g/cm(2); P < .001 24 months: 1.11 ± 0.19 g/cm(2); P < .001) and the FN (12 months: 0.88 ± 0.16 g/cm(2); P < .002 24 months: 0.90 ± 0.15 g/cm(2); P < .001) in comparison with baseline pre-OLT (LS pre-OLT 0.98 ± 0.19 g/cm(2), FN 0.86 ± 0.14 g/cm(2)). The overall bone fracture rate was 5.4% up to 24 months. CONCLUSION: Ibandronate once monthly per os significantly increased the BMD in the LS and FN after OLT at 12 and 24 months. The increased BMD limits the risk of fracture.

Electrophilic PPARγ ligands inhibit corneal fibroblast to myofibroblast differentiation in vitro: a potentially novel therapy for corneal scarring.

A critical component of corneal scarring is the TGFß-induced differentiation of corneal keratocytes into myofibroblasts. Inhibitors of this differentiation are potentially therapeutic for corneal scarring. In this study, we tested the relative effectiveness and mechanisms of action of two electrophilic peroxisome proliferator-activated receptor gamma (PPARγ) ligands: cyano-3,12-dioxolean-1,9-dien-28-oic acid-methyl ester (CDDO-Me) and 15-deoxy-Δ(-12,14)-prostaglandin J(2) (15d-PGJ(2)) for inhibiting TGFß-induced myofibroblast differentiation in vitro. TGFß was used to induce myofibroblast differentiation in cultured, primary human corneal fibroblasts. CDDO-Me and 15d-PGJ(2) were added to cultures to test their ability to inhibit this process. Myofibroblast differentiation was assessed by measuring the expression of myofibroblast-specific proteins (αSMA, collagen I, and fibronectin) and mRNA (αSMA and collagen III). The role of PPARγ in the inhibition of myofibroblast differentiation by these agents was tested in genetically and pharmacologically manipulated cells. Finally, we assayed the importance of electrophilicity in the actions of these agents on TGFß-induced αSMA expression via Western blotting and immunofluorescence. Both electrophilic PPARγ ligands (CDDO-Me and 15d-PGJ(2)) potently inhibited TGFß-induced myofibroblast differentiation, but PPARγ was only partially required for inhibition of myofibroblast differentiation by either agent. Electrophilic PPARγ ligands were able to inhibit myofibroblast differentiation more potently than non-electrophilic PPARγ ligands, suggesting an important role of electrophilicity in this process. CDDO-Me and 15d-PGJ(2) are strong inhibitors of TGFß-induced corneal fibroblast to myofibroblast differentiation in vitro, suggesting this class of agents as potential novel therapies for corneal scarring warranting further study in pre-clinical animal models.