RÉSUMÉ
Visual System Homeobox 2 (Vsx2) is a transcription factor expressed in the developing retina that regulates tissue identity, growth, and fate determination. Several mutations in the Vsx2 gene exist in mice, including a spontaneous nonsense mutation and two targeted missense mutations originally identified in humans. Here, we expand the genetic repertoire to include a LacZ reporter allele (Vsx2 LacZ ) designed to express beta-Galactosidase (b-GAL) and simultaneously disrupt Vsx2 function (knock-in/knock-out). The retinal expression pattern of b-GAL is concordant with VSX2, and the mutant allele is recessive. Vsx2 LacZ homozygous mice have congenital bilateral microphthalmia accompanied by defects in retinal development including ectopic expression of non-retinal genes, reduced proliferation, delayed neurogenesis, aberrant tissue morphology, and an absence of bipolar interneurons - all hallmarks of Vsx2 loss-of-function. Unexpectedly, the mutant VSX2 protein is stably expressed, and there are subtle differences in eye size and early retinal neurogenesis when compared to the null mutant, ocular retardation J. We propose that b-GAL expression from the Vsx2 LacZ allele is a reliable reporter of VSX2 expression and that the allele exhibits loss-of-function characteristics. However, the perdurance of the mutant VSX2 protein combined with subtle deviations from the null phenotype leaves open the possibility that Vsx2 LacZ allele is not a complete knock-out. The Vsx2 LacZ allele adds to the genetic toolkit for understanding Vsx2 function.
RÉSUMÉ
Laser-induced photodamage is a robust method for investigating retinal pathologies in small animals. However, aiming of the photocoagulation laser is often limited by manual alignment and lacks real-time feedback on lesion location and severity. Here, we demonstrate a multimodality OCT and SLO ophthalmic imaging system with an image-guided scanning laser lesioning module optimized for the murine retina. The proposed system enables targeting of focal and extended area lesions under OCT guidance to benefit visualization of photodamage response and the precision and repeatability of laser lesion models of retinal injury.
RÉSUMÉ
BACKGROUND: A goal of developmental genetics is to identify functional interactions that underlie phenotypes caused by mutations. We sought to identify functional interactors of Vsx2, which when mutated, disrupts early retinal development. We utilized the Vsx2 loss-of-function mouse, ocular retardation J (orJ), to assess interactions based on principles of positive and negative epistasis as applied to bulk transcriptome data. This was first tested in vivo with Mitf, a target of Vsx2 repression, and then to cultures of orJ retina treated with inhibitors of Retinoid-X Receptors (RXR) to target Rxrg, an up-regulated gene in the orJ retina, and gamma-Secretase, an enzyme required for Notch signaling, a key mediator of retinal proliferation and neurogenesis. RESULTS: Whereas Mitf exhibited robust positive epistasis with Vsx2, it only partially accounts for the orJ phenotype, suggesting other functional interactors. RXR inhibition yielded minimal evidence for epistasis between Vsx2 and Rxrg. In contrast, gamma-Secretase inhibition caused hundreds of Vsx2-dependent genes associated with proliferation to deviate further from wild-type, providing evidence for convergent negative epistasis with Vsx2 in regulating tissue growth. CONCLUSIONS: Combining in vivo and ex vivo testing with transcriptome analysis revealed quantitative and qualitative characteristics of functional interaction between Vsx2, Mitf, RXR, and gamma-Secretase activities.
Sujet(s)
Protéines à homéodomaine , Facteurs de transcription , Souris , Animaux , Facteurs de transcription/génétique , Protéines à homéodomaine/génétique , Amyloid precursor protein secretases/génétique , Rétine , Neurogenèse/physiologieRÉSUMÉ
An important question in organogenesis is how tissue-specific transcription factors interact with signaling pathways. In some cases, transcription factors define the context for how signaling pathways elicit tissue- or cell-specific responses, and in others, they influence signaling through transcriptional regulation of signaling components or accessory factors. We previously showed that during optic vesicle patterning, the Lim-homeodomain transcription factor Lhx2 has a contextual role by linking the Sonic Hedgehog (Shh) pathway to downstream targets without regulating the pathway itself. Here, we show that during early retinal neurogenesis in mice, Lhx2 is a multilevel regulator of Shh signaling. Specifically, Lhx2 acts cell autonomously to control the expression of pathway genes required for efficient activation and maintenance of signaling in retinal progenitor cells. The Shh co-receptors Cdon and Gas1 are candidate direct targets of Lhx2 that mediate pathway activation, whereas Lhx2 directly or indirectly promotes the expression of other pathway components important for activation and sustained signaling. We also provide genetic evidence suggesting that Lhx2 has a contextual role by linking the Shh pathway to downstream targets. Through these interactions, Lhx2 establishes the competence for Shh signaling in retinal progenitors and the context for the pathway to promote early retinal neurogenesis. The temporally distinct interactions between Lhx2 and the Shh pathway in retinal development illustrate how transcription factors and signaling pathways adapt to meet stage-dependent requirements of tissue formation.
Sujet(s)
Protéines Hedgehog , Neurogenèse , Souris , Animaux , Neurogenèse/génétique , Rétine , Transduction du signal , Facteurs de transcription , Protéines à homéodomaine LIM/génétiqueRÉSUMÉ
Retinitis pigmentosa (RP), a retinal degenerative disease, is the leading cause of heritable blindness. Previously, we described that Arap1-/- mice develop a similar pattern of photoreceptor degeneration. Arap1 is an Arf-directed GTPase-activating protein shown to modulate actin cytoskeletal dynamics. Curiously, Arap1 expression was detected in Müller glia and retinal pigment epithelium (RPE), but not the photoreceptors themselves. In this study, we generated conditional knockout mice for Müller glia/RPE, Müller glia and RPE via targeting Rlbp1, Glast and Vmd2 promoters, respectively, to drive Cre recombinase expression to knock out Arap1. Vmd2-Cre Arap1tm1c/tm1c and Rlbp1-Cre Arap1tm1c/tm1c mice, but not Glast-Cre Arap1tm1c/tm1c mice, recapitulated the phenotype originally observed in germline Arap1-/- mice. Mass spectrometry analysis of human ARAP1 co-immunoprecipitation identified candidate binding partners of ARAP1, revealing potential interactants involved in phagocytosis, cytoskeletal composition, intracellular trafficking and endocytosis. Quantification of outer segment phagocytosis in vivo demonstrated a clear phagocytic defect in Arap1-/- mice compared to Arap1+/+ controls. We conclude that Arap1 expression in RPE is necessary for photoreceptor survival due to its indispensable function in RPE phagocytosis. This article has an associated First Person interview with the first author of the paper.
Sujet(s)
Dégénérescence de la rétine , Rétinite pigmentaire , Protéines adaptatrices de la transduction du signal , Animaux , Protéines de transport/métabolisme , Protéines d'activation de la GTPase/métabolisme , Humains , Souris , Souris knockout , Phagocytose , Rétine/métabolisme , Dégénérescence de la rétine/métabolisme , Épithélium pigmentaire de la rétine/métabolisme , Rétinite pigmentaire/métabolismeRÉSUMÉ
OBJECTIVE: The Hedgehog pathway is a fundamental signaling pathway in organogenesis. The expression patterns of the ligand Sonic Hedgehog (Shh) and key pathway components have been studied in many tissues but direct spatial comparisons across tissues with different cell compositions and structural organization are not common and could reveal tissue-specific differences in pathway dynamics. RESULTS: We directly compared the expression characteristics of Shh, and four genes with functional roles in signaling and whose expression levels serve as readouts of pathway activity in multiple tissues of the embryonic mouse head at embryonic day 15.5 by serial in situ hybridization. The four readout genes were the positive feedback regulator Gli1, and three negative feedback regulators, Patched1, Patched2, and Hedgehog Interacting Protein. While the relative abundance of Gli1 was similar across tissues, the relative expression levels and spatial distribution of Shh and the negative feedback regulators differed, suggesting that feedback regulation of hedgehog signaling is context dependent. This comparative analysis offers insight into how consistent pathway activity could be achieved in tissues with different morphologies and characteristics of ligand expression.
Sujet(s)
Tête/embryologie , Protéines Hedgehog , Transduction du signal , Animaux , Rétrocontrôle physiologique , Protéines Hedgehog/génétique , Souris , Protéine à doigt de zinc GLI1/génétiqueRÉSUMÉ
Ongoing research using cell transplantation and viral-mediated gene therapy has been making progress to restore vision by retinal repair, but targeted delivery and complete cellular integration remain challenging. An alternative approach is to induce endogenous Müller glia (MG) to regenerate lost neurons and photoreceptors, as occurs spontaneously in teleost fish and amphibians. Extracellular vesicles (EVs) can transfer protein and RNA cargo between cells serving as a novel means of cell-cell communication. We conducted an in vivo screen in zebrafish to identify sources of EVs that could induce MG to dedifferentiate and generate proliferating progenitor cells after intravitreal injection into otherwise undamaged zebrafish eyes. Small EVs (sEVs) from C6 glioma cells were the most consistent at inducing MG-derived proliferating cells. Ascl1a expression increased after intravitreal injection of C6 sEVs and knockdown of ascl1a inhibited the induction of proliferation. Proteomic and RNAseq analyses of EV cargo content were performed to begin to identify key factors that might target EVs to MG and initiate retina regeneration.
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Vésicules extracellulaires , Neurogenèse , Cellules photoréceptrices d'invertébré/métabolisme , Protéomique/méthodes , Rétine/métabolisme , Protéines de poisson-zèbre/métabolisme , Animaux , Animal génétiquement modifié , Prolifération cellulaire , Cellules cultivées , Injections , Cellules photoréceptrices d'invertébré/cytologie , Rétine/cytologie , Danio zébréRÉSUMÉ
Purpose: The adult mammalian retina is typically incapable of regeneration when damaged by disease or trauma. Restoration of function would require generation of new adult neurons, something that until recently, mammals were thought to be incapable of doing. However, previous studies from this laboratory have shown that the α7 nicotinic acetylcholine receptor (α7 nAChR) agonist, PNU-282987, induces cell cycle reentry of Müller glia and generation of mature retinal neurons in adult rats, in the absence of detectible injury. This study analyzes how PNU-282987 treatment in RPE leads to robust BrdU incorporation in Müller glia in adult mice and leads to generation of Müller-derived retinal progenitors and neuronal differentiation. Methods: Retinal BrdU incorporation was examined after eye drop application of PNU-282987 in adult wild-type and transgenic mice that contain tamoxifen-inducible tdTomato Müller glia, or after intraocular injection of conditioned medium from PNU-282987-treated cultured RPE cells. Results: PNU-282987 induced robust incorporation of BrdU in all layers of the adult mouse retina. The α7 nAChR agonist was found to stimulate cell cycle reentry of Müller glia and their generation of new retinal progenitors indirectly, via the RPE, in an α7 nAChR-dependent fashion. Conclusions: The results from this study point to RPE as a contributor to Müller glial neurogenic responses. The manipulation of the RPE to stimulate retinal neurogenesis offers a new direction for developing novel and potentially transformative treatments to reverse the loss of neurons associated with neurodegenerative disease, traumatic injury, or aging.
Sujet(s)
Benzamides/pharmacologie , Composés bicycliques pontés/pharmacologie , Cellules épendymogliales/physiologie , Neurogenèse/physiologie , Névroglie/physiologie , Agonistes nicotiniques/pharmacologie , Épithélium pigmentaire de la rétine/effets des médicaments et des substances chimiques , Récepteur nicotinique de l'acétylcholine alpha7/agonistes , Animaux , Broxuridine/métabolisme , Cycle cellulaire/physiologie , Cellules cultivées , Cellules épendymogliales/cytologie , Femelle , Mâle , Souris , Souris transgéniques , Facteur de transcription PAX6/métabolisme , Rats , Épithélium pigmentaire de la rétine/cytologie , Épithélium pigmentaire de la rétine/métabolisme , Cellules souches/physiologieRÉSUMÉ
The optic neuroepithelial continuum of vertebrate eye develops into three differentially growing compartments: the retina, the ciliary margin (CM), and the retinal pigment epithelium (RPE). Neurofibromin 2 (Nf2) is strongly expressed in slowly expanding RPE and CM compartments, and the loss of mouse Nf2 causes hyperplasia in these compartments, replicating the ocular abnormalities seen in human NF2 patients. The hyperplastic ocular phenotypes were largely suppressed by heterozygous deletion of Yap and Taz, key targets of the Nf2-Hippo signaling pathway. We also found that, in addition to feedback transcriptional regulation of Nf2 by Yap/Taz in the CM, activation of Nf2 expression by Mitf in the RPE and suppression by Sox2 in retinal progenitor cells are necessary for the differential growth of the corresponding cell populations. Together, our findings reveal that Nf2 is a key player that orchestrates the differential growth of optic neuroepithelial compartments during vertebrate eye development.
Sujet(s)
Cils vibratiles/physiologie , Hyperplasie/anatomopathologie , Cellules souches neurales/cytologie , Neurofibromine-2/physiologie , Organogenèse/physiologie , Épithélium pigmentaire de la rétine/cytologie , Acyltransferases , Protéines adaptatrices de la transduction du signal/génétique , Protéines adaptatrices de la transduction du signal/métabolisme , Animaux , Protéines du cycle cellulaire , Lignage cellulaire , Polarité de la cellule , Cellules cultivées , Régulation de l'expression des gènes au cours du développement , Voie de signalisation Hippo , Humains , Hyperplasie/métabolisme , Souris , Souris knockout , Cellules souches neurales/physiologie , Phénotype , Phosphoprotéines/génétique , Phosphoprotéines/métabolisme , Protein-Serine-Threonine Kinases/génétique , Protein-Serine-Threonine Kinases/métabolisme , Épithélium pigmentaire de la rétine/physiologie , Facteurs de transcription/génétique , Facteurs de transcription/métabolisme , Protéines de signalisation YAPRÉSUMÉ
To better understand the roles of microRNAs in glial function, we used a conditional deletion of Dicer1 (Dicer-CKOMG) in retinal Müller glia (MG). Dicer1 deletion from the MG leads to an abnormal migration of the cells as early as 1 month after the deletion. By 6 months after Dicer1 deletion, the MG form large aggregations and severely disrupt normal retinal architecture and function. The most highly upregulated gene in the Dicer-CKOMG MG is the proteoglycan Brevican (Bcan) and overexpression of Bcan results in similar aggregations of the MG in wild-type retina. One potential microRNA that regulates Bcan is miR-9, and overexpression of miR-9 can partly rescue the effects of Dicer1 deletion on the MG phenotype. We also find that MG from retinitis pigmentosa patients display an increase in Brevican immunoreactivity at sites of MG aggregation, linking the retinal remodeling that occurs in chronic disease with microRNAs.
Sujet(s)
Cellules épendymogliales/métabolisme , Homéostasie/génétique , microARN/génétique , Névroglie/métabolisme , Rétine/métabolisme , Cellules 3T3 , Animaux , Mouvement cellulaire/génétique , Cellules cultivées , DEAD-box RNA helicases/déficit , DEAD-box RNA helicases/génétique , Analyse de profil d'expression de gènes , Souris , Souris knockout , Souris transgéniques , Microscopie confocale , Névroglie/cytologie , Rétine/cytologie , Ribonuclease III/déficit , Ribonuclease III/génétiqueRÉSUMÉ
While innate behaviors are conserved throughout the animal kingdom, it is unknown whether common signaling pathways regulate the development of neuronal populations mediating these behaviors in diverse organisms. Here, we demonstrate that the Wnt/ß-catenin effector Lef1 is required for the differentiation of anxiolytic hypothalamic neurons in zebrafish and mice, although the identity of Lef1-dependent genes and neurons differ between these 2 species. We further show that zebrafish and Drosophila have common Lef1-dependent gene expression in their respective neuroendocrine organs, consistent with a conserved pathway that has diverged in the mouse. Finally, orthologs of Lef1-dependent genes from both zebrafish and mouse show highly correlated hypothalamic expression in marmosets and humans, suggesting co-regulation of 2 parallel anxiolytic pathways in primates. These findings demonstrate that during evolution, a transcription factor can act through multiple mechanisms to generate a common behavioral output, and that Lef1 regulates circuit development that is fundamentally important for mediating anxiety in a wide variety of animal species.
Sujet(s)
Anxiété/prévention et contrôle , Hypothalamus/métabolisme , Facteur de transcription LEF-1/métabolisme , Protéines de tissu nerveux/métabolisme , Neurogenèse , Neurones/métabolisme , Facteurs de transcription/métabolisme , Protéines de poisson-zèbre/métabolisme , Animaux , Anxiété/métabolisme , Anxiété/anatomopathologie , Comportement animal , Marqueurs biologiques/métabolisme , Protéines de Drosophila/génétique , Protéines de Drosophila/métabolisme , Drosophila melanogaster , Femelle , Régulation de l'expression des gènes , Gènes rapporteurs , Humains , Hypothalamus/cytologie , Hypothalamus/anatomopathologie , Facteur de transcription LEF-1/génétique , Mâle , Souris knockout , Souris transgéniques , Mutation , Protéines de tissu nerveux/génétique , Neurones/cytologie , Neurones/anatomopathologie , Spécificité d'espèce , Facteurs de transcription/génétique , Danio zébré , Protéines de poisson-zèbre/génétiqueRÉSUMÉ
The visual responses of vertebrates are sensitive to the overall composition of retinal interneurons including amacrine cells, which tune the activity of the retinal circuitry. The expression of Paired-homeobox 6 (PAX6) is regulated by multiple cis-DNA elements including the intronic α-enhancer, which is active in GABAergic amacrine cell subsets. Here, we report that the transforming growth factor ß1-induced transcript 1 protein (Tgfb1i1) interacts with the LIM domain transcription factors Lhx3 and Isl1 to inhibit the α-enhancer in the post-natal mouse retina. Tgfb1i1-/- mice show elevated α-enhancer activity leading to overproduction of Pax6ΔPD isoform that supports the GABAergic amacrine cell fate maintenance. Consequently, the Tgfb1i1-/- mouse retinas show a sustained light response, which becomes more transient in mice with the auto-stimulation-defective Pax6ΔPBS/ΔPBS mutation. Together, we show the antagonistic regulation of the α-enhancer activity by Pax6 and the LIM protein complex is necessary for the establishment of an inner retinal circuitry, which controls visual adaptation.
Sujet(s)
Protéines du cytosquelette/métabolisme , Protéines de liaison à l'ADN/métabolisme , Éléments activateurs (génétique) , Régulation de l'expression des gènes , Protéines à domaine LIM/métabolisme , Protéines à homéodomaine LIM/métabolisme , Facteur de transcription PAX6/métabolisme , Rétine/physiologie , Facteurs de transcription/métabolisme , Adaptation oculaire , Animaux , Souris , Souris knockoutRÉSUMÉ
Attractive growth cone turning requires Igf2bp1-dependent local translation of ß-actin mRNA in response to external cues in vitro. While in vivo studies have shown that Igf2bp1 is required for cell migration and axon terminal branching, a requirement for Igf2bp1 function during axon outgrowth has not been demonstrated. Using a timelapse assay in the zebrafish retinotectal system, we demonstrate that the ß-actin 3'UTR is sufficient to target local translation of the photoconvertible fluorescent protein Kaede in growth cones of pathfinding retinal ganglion cells (RGCs) in vivo. Igf2bp1 knockdown reduced RGC axonal outgrowth and tectal coverage and retinal cell survival. RGC-specific expression of a phosphomimetic Igf2bp1 reduced the density of axonal projections in the optic tract while sparing RGCs, demonstrating for the first time that Igf2bp1 is required during axon outgrowth in vivo. Therefore, regulation of local translation mediated by Igf2bp proteins may be required at all stages of axon development.
Sujet(s)
Axones/physiologie , Protéines de liaison à l'ARN/physiologie , Cellules ganglionnaires rétiniennes/physiologie , Protéines de poisson-zèbre/physiologie , Actines/physiologie , Animaux , Techniques de knock-down de gènes , Danio zébré/croissance et développement , Danio zébré/physiologieRÉSUMÉ
BACKGROUND: Vertebrate retinal development is a complex process, requiring the specification and maintenance of retinal identity, proliferative expansion of retinal progenitor cells (RPCs), and their differentiation into retinal neurons and glia. The homeobox gene Vsx2 is expressed in RPCs and required for the proper execution of this retinal program. However, our understanding of the mechanisms by which Vsx2 does this is still rudimentary. To define the autonomy requirements for Vsx2 in the regulation of RPC properties, we generated chimeric mouse embryos comprised of wild-type and Vsx2-deficient cells. RESULTS: We show that Vsx2 maintains retinal identity in part through the cell-autonomous repression of the retinal pigment epithelium determinant Mitf, and that Lhx2 is required cell autonomously for the ectopic Mitf expression in Vsx2-deficient cells. We also found significant cell-nonautonomous contributions to Vsx2-mediated regulation of RPC proliferation, pointing to an important role for Vsx2 in establishing a growth-promoting extracellular environment. Additionally, we report a cell-autonomous requirement for Vsx2 in controlling when neurogenesis is initiated, indicating that Vsx2 is an important mediator of neurogenic competence. Finally, the distribution of wild-type cells shifted away from RPCs and toward retinal ganglion cell precursors in patches of high Vsx2-deficient cell density to potentially compensate for the lack of fated precursors in these areas. CONCLUSIONS: Through the generation and analysis of genetic chimeras, we demonstrate that Vsx2 utilizes both cell-autonomous and cell-nonautonomous mechanisms to regulate progenitor properties in the embryonic retina. Importantly, Vsx2's role in regulating Mitf is in part separable from its role in promoting proliferation, and proliferation is excluded as the intrinsic timer that determines when neurogenesis is initiated. These findings highlight the complexity of Vsx2 function during retinal development and provide a framework for identifying the molecular mechanisms mediating these functions.
Sujet(s)
Protéines à homéodomaine/physiologie , Facteur de transcription associé à la microphtalmie/physiologie , Cellules souches neurales/métabolisme , Neurogenèse/génétique , Rétine/embryologie , Facteurs de transcription/physiologie , Animaux , Division cellulaire , Chimère , Transfert d'embryon , Femelle , Gènes rapporteurs , Protéines à homéodomaine/génétique , Protéines à homéodomaine LIM/physiologie , Mâle , Souris , Souris transgéniques , Facteur de transcription associé à la microphtalmie/biosynthèse , Facteur de transcription associé à la microphtalmie/génétique , Mosaïcisme , Névroglie/cytologie , Spécificité d'organe , Rétine/cytologie , Cellules ganglionnaires rétiniennes/cytologie , Facteurs de transcription/déficit , Facteurs de transcription/génétiqueRÉSUMÉ
The retinal pigment epithelium (RPE) is a simple epithelium interposed between the neural retina and the choroid. Although only 1 cell-layer in thickness, the RPE is a virtual workhorse, acting in several capacities that are essential for visual function and preserving the structural and physiological integrities of neighboring tissues. Defects in RPE function, whether through chronic dysfunction or age-related decline, are associated with retinal degenerative diseases including age-related macular degeneration. As such, investigations are focused on developing techniques to replace RPE through stem cell-based methods, motivated primarily because of the seemingly limited regeneration or self-repair properties of mature RPE. Despite this, RPE cells have an unusual capacity to transdifferentiate into various cell types, with the particular fate choices being highly context-dependent. In this review, we describe recent findings elucidating the mechanisms and steps of RPE development and propose a developmental framework for understanding the apparent contradiction in the capacity for low self-repair versus high transdifferentiation.
Sujet(s)
Homéostasie/physiologie , Régénération/physiologie , Épithélium pigmentaire de la rétine/cytologie , Épithélium pigmentaire de la rétine/physiologie , Animaux , Transdifférenciation cellulaire , Humains , Transplantation de cellules souchesRÉSUMÉ
The LIM-Homeodomain transcription factor Lhx2 is an essential organizer of early eye development and is subsequently expressed in retinal progenitor cells (RPCs). To determine its requirement in RPCs, we performed a temporal series of conditional inactivations in mice with the early RPC driver Pax6 α-Cre and the tamoxifen-inducible Hes1(CreERT2) driver. Deletion of Lhx2 caused a significant reduction of the progenitor population and a corresponding increase in neurogenesis. Precursor fate choice correlated with the time of inactivation; early and late inactivation led to the overproduction of retinal ganglion cells (RGCs) and rod photoreceptors, respectively. In each case, however, the overproduction was selective, occurring at the expense of other cell types and indicating a role for Lhx2 in generating cell type diversity. RPCs that persisted in the absence of Lhx2 continued to generate RGC precursors beyond their normal production window, suggesting that Lhx2 facilitates a transition in competence state. These results identify Lhx2 as a key regulator of RPC properties that contribute to the ordered production of multiple cell types during retinal tissue formation.
Sujet(s)
Régulation de l'expression des gènes au cours du développement , Protéines à homéodomaine LIM/génétique , Protéines à homéodomaine LIM/physiologie , Cellules souches neurales/physiologie , Neurogenèse/physiologie , Rétine/embryologie , Facteurs de transcription/génétique , Facteurs de transcription/physiologie , Animaux , Différenciation cellulaire/physiologie , Femelle , Techniques de knock-in de gènes , Mâle , Souris , Souches mutantes de souris , Cellules souches neurales/cytologie , Grossesse , Rétine/cytologie , Rétine/croissance et développement , Rétine/physiologie , Cellules ganglionnaires rétiniennes/cytologie , Cellules ganglionnaires rétiniennes/physiologie , Cellules photoréceptrices en bâtonnet de la rétine/cytologie , Cellules photoréceptrices en bâtonnet de la rétine/physiologieRÉSUMÉ
Non-mammalian vertebrates have a robust ability to regenerate injured retinal neurons from Müller glia (MG) that activate the gene encoding the proneural factor Achaete-scute homolog 1 (Ascl1; also known as Mash1 in mammals) and de-differentiate into progenitor cells. By contrast, mammalian MG have a limited regenerative response and fail to upregulate Ascl1 after injury. To test whether ASCL1 could restore neurogenic potential to mammalian MG, we overexpressed ASCL1 in dissociated mouse MG cultures and intact retinal explants. ASCL1-infected MG upregulated retinal progenitor-specific genes and downregulated glial genes. Furthermore, ASCL1 remodeled the chromatin at its targets from a repressive to an active configuration. MG-derived progenitors differentiated into cells that exhibited neuronal morphologies, expressed retinal subtype-specific neuronal markers and displayed neuron-like physiological responses. These results indicate that a single transcription factor, ASCL1, can induce a neurogenic state in mature MG.
Sujet(s)
Facteurs de transcription à motif basique hélice-boucle-hélice/métabolisme , Névroglie/métabolisme , Régénération , Rétine/cytologie , Neurones rétiniens/cytologie , Animaux , Facteurs de transcription à motif basique hélice-boucle-hélice/génétique , Marqueurs biologiques/métabolisme , Prolifération cellulaire , Cellules cultivées , Reprogrammation cellulaire , Assemblage et désassemblage de la chromatine , Clonage moléculaire , Facteur de croissance épidermique/pharmacologie , Régulation de l'expression des gènes , Cellules HEK293 , Histone/métabolisme , Humains , Techniques in vitro , Lentivirus/génétique , Lentivirus/métabolisme , Protéines luminescentes/métabolisme , Souris , Souris de lignée C57BL , Neurogenèse , Névroglie/cytologie , Techniques de patch-clamp , Rétine/métabolisme , Neurones rétiniens/effets des médicaments et des substances chimiques , Neurones rétiniens/métabolisme ,RÉSUMÉ
The small GTPase CDC42 has pleiotropic functions during development and in the adult. These functions include intra- as well as intercellular tasks such as organization of the cytoskeleton and, at least in epithelial cells, formation of adherens junctions. To investigate CDC42 in the neuronal retina, we generated retina-specific Cdc42-knockdown mice (Cdc42-KD) and analyzed the ensuing consequences for the developing and postnatal retina. Lack of CDC42 affected organization of the developing retina as early as E17.5, prevented correct tissue lamination, and resulted in progressive retinal degeneration and severely reduced retinal function of the postnatal retina. Despite the disorganization of the retina, formation of the primary vascular plexus was not strongly affected. However, both deeper vascular plexi developed abnormally with no clear layering of the vessels. Retinas of Cdc42-KD mice showed increased expression of pro-survival, but also of pro-apoptotic and pro-inflammatory genes and exhibited prolonged Müller glia hypertrophy. Thus, functional CDC42 is important for correct tissue organization already during retinal development. Its absence leads to severe destabilization of the postnatal retina with strong degeneration and loss of retinal function.
Sujet(s)
Microvaisseaux/embryologie , Morphogenèse/physiologie , Rétine/embryologie , Protéine G cdc42/génétique , Animaux , Animaux nouveau-nés , Survie cellulaire , Embryon de mammifère , Expression des gènes , Analyse de profil d'expression de gènes , Souris , Souris knockout , Microvaisseaux/métabolisme , Microvaisseaux/anatomopathologie , Névroglie/métabolisme , Névroglie/anatomopathologie , Neurones/métabolisme , Neurones/anatomopathologie , Rétine/métabolisme , Rétine/anatomopathologie , Protéine G cdc42/métabolismeRÉSUMÉ
The homeodomain and adjacent CVC domain in the visual system homeobox (VSX) proteins are conserved from nematodes to humans. Humans with missense mutations in these regions of VSX2 have microphthalmia, suggesting both regions are critical for function. To assess this, we generated the corresponding mutations in mouse Vsx2. The homeodomain mutant protein lacked DNA binding activity and the knock-in mutant phenocopied the null mutant, ocular retardation J. The CVC mutant protein exhibited weakened DNA binding; and, although the corresponding knock-in allele was recessive, it unexpectedly caused the strongest phenotype, as indicated by severe microphthalmia and hyperpigmentation of the neural retina. This occurred through a cryptic transcriptional feedback loop involving the transcription factors Mitf and Otx1 and the Cdk inhibitor p27(Kip1). Our data suggest that the phenotypic severity of the CVC mutant depends on the weakened DNA binding activity elicited by the CVC mutation and a previously unknown protein interaction between Vsx2 and its regulatory target Mitf. Our data also suggest that an essential function of the CVC domain is to assist the homeodomain in high-affinity DNA binding, which is required for eye organogenesis and unhindered execution of the retinal progenitor program in mammals. Finally, the genetic and phenotypic behaviors of the CVC mutation suggest it has the characteristics of a recessive neomorph, a rare type of genetic allele.
Sujet(s)
Protéines de liaison à l'ADN/génétique , Oeil/croissance et développement , Protéines à homéodomaine/génétique , Facteur de transcription associé à la microphtalmie , Organogenèse/génétique , Facteurs de transcription/génétique , Allèles , Animaux , Inhibiteur p27 de kinase cycline-dépendante/génétique , Régulation de l'expression des gènes au cours du développement , Cellules HEK293 , Protéines à homéodomaine/métabolisme , Humains , Souris , Souris transgéniques , Facteur de transcription associé à la microphtalmie/génétique , Facteur de transcription associé à la microphtalmie/métabolisme , Mutation/génétique , Facteurs de transcription Otx/génétique , Structure tertiaire des protéines/génétique , Rétine/croissance et développement , Rétine/métabolisme , Facteurs de transcription/métabolismeRÉSUMÉ
BACKGROUND: The cell-cycle regulator Cyclin D1 is expressed in embryonic retinal progenitor cells (RPCs) and regulates their cell-cycle rate and neurogenic output. We report here that Cyclin D1 also has important functions in postnatal retinal histogenesis. RESULTS: The initial production of Müller glia and bipolar cells was enhanced in Cyclin D1 knockout (Ccnd1(-/-) ) retinas. Despite a steeper than normal rate of depletion of the RPC population at embryonic ages, postnatal Ccnd1(-/-) retinas exhibited an extended window of proliferation, neurogenesis, and gliogenesis. Cyclin D3, normally confined to Müller glia, was prematurely expressed in Ccnd1(-/-) RPCs. However, Cyclin D3 did not compensate for Cyclin D1 in regulating cell-cycle kinetics or neurogenic output. CONCLUSIONS: The data presented in this study along with our previous finding that Cyclin D2 was unable to completely compensate for the absence of Cyclin D1 indicate that Cyclin D1 regulates retinal histogenesis in ways not shared by the other D-cyclins.