RÉSUMÉ
Composite membranes were prepared from lignin alkali (LA), polyvinyl alcohol (PVA), and cellulose nanofibrils (CNF) using a simple, low-cost, and environmentally friendly method. The deodorization performances and structures of these membranes were also characterized. The sample referred to as L3C3P5 prepared with a solution containing 35.7â wt% LA, 53.6â wt% PVA, and 10.7â wt% CNF showed the best deodorization properties, and the H2S adsorption time reached 36â min. The adsorption performance was further improved by adding nano-CuO to the membrane, and the H2S adsorption time of the doped membrane L3C3P5C4 reached 60â min. While the H2S adsorption performance improved, structural analysis revealed that the addition of nano-CuO reduced the crystallinity in the membrane, caused the membrane to crack, and led to a decrease in the mechanical properties. The surface oxygens in the L3C3P5C4 membrane were primarily C-O bonds and lattice oxygens in CuO. After the H2S adsorption reaction, the lattice oxygen disappeared, and CuS products appeared.
Sujet(s)
Cellulose , Lignine , Lignine/composition chimique , Cellulose/composition chimique , Poly(alcool vinylique) , AdsorptionRÉSUMÉ
BACKGROUND: Yungui Plateau in Southwest China is characterized by multi-language and multi-ethnic communities and is one of the regions with the wealthiest ethnolinguistic, cultural and genetic diversity in East Asia. There are numerous Tai-Kadai (TK)-speaking populations, but their detailed evolutionary history and biological adaptations are still unclear. RESULTS: Here, we genotyped genome-wide SNP data of 77 unrelated TK-speaking Zhuang and Dong individuals from the Yungui Plateau and explored their detailed admixture history and adaptive features using clustering patterns, allele frequency differentiation and sharing haplotype patterns. TK-speaking Zhuang and Dong people in Guizhou are closely related to geographically close TK and Hmong-Mien (HM)-speaking populations. Besides, we identified that Guizhou TK-speaking people have a close genetic relationship with Austronesian (AN)-speaking Atayal and Paiwan people, which is supported by the common origin of the ancient Baiyue tribe. We additionally found subtle genetic differences among the newly studied TK people and previously reported Dais via the fine-scale genetic substructure analysis based on the shared haplotype chunks. Finally, we identified specific selection candidate signatures associated with several essential human immune systems and neurological disorders, which could provide evolutionary evidence for the allele frequency distribution pattern of genetic risk loci. CONCLUSIONS: Our comprehensive genetic characterization of TK people suggested the strong genetic affinity within TK groups and extensive gene flow with geographically close HM and Han people. We also provided genetic evidence that supported the common origin hypothesis of TK and AN people. The best-fitted admixture models further suggested that ancestral sources from northern millet farmers and southern inland and coastal people contributed to the formation of the gene pool of the Zhuang and Dong people.
Sujet(s)
Adaptation biologique , Asiatiques , Humains , Asiatiques/génétique , Évolution biologique , Chine , Analyse de regroupements , Génétique des populationsRÉSUMÉ
Vitellogenin (Vg) is the major precursor of the egg-yolk proteins, which mainly acts as an energy reserve molecule for providing nutrients during embryonic development. Vg also plays an immune function in vertebrates such as fish, but there are few studies on the immune function of Vg in invertebrates. In the present study, a Vg homologue (CgVg) was identified and characterized in oyster Crassostrea gigas. There are three domains in the CgVg protein, including a Vitellogenin_N domain, a domain of unknown function 1943 (DUF1943) and a von Willebrand factor type D domain (VWD). The mRNA transcripts of CgVg were detected in all tested tissues with high expression in the gonad, hepatopancreas and haemocytes, which was 466.29-, 117.15- and 57.49-fold (p < 0.01) of that in adductor muscle, respectively. After Vibrio splendidus stimulation, the mRNA expression level of CgVg in haemocytes increased significantly at 6, 12 and 24 h, which was 1.97-, 3.58- and 1.3-fold (p < 0.01) of that in the seawater group, respectively. The immunofluorescence assay showed that positive signals of CgVg protein were mainly located at the cytoplasm of haemocytes. The recombinant protein of DUF1943 domain (rDUF1943) and VWD domain (rVWD) was able to bind lipopolysaccharide (LPS), mannose (MAN), peptidoglycan (PGN) and poly (I:C), as well as Gram-positive bacteria (Staphylococcus aureus and Micrococcus luteus), Gram-negative bacteria (Escherichia coli and V. splendidus) and fungi (Pichia pastoris). rDUF1943 exhibited stronger agglutination activity towards S. aureus, M. luteus, E. coli, V. splendidus and P. pastoris, while agglutination was only observed in the rVWD group towards P. pastoris. The rVWD inhibited the growth of E. coli, S. aureus and V. splendidus, while no antibacterial activity was detected in rDUF1943 group. Collectively, CgVg not only functioned as a pattern recognition receptor (PRR) to bind various microorganisms and PAMPs, but also as an immune effector participating in the clearance of invaders, in which DUF1943 and VWD domain were mainly responsible for agglutinating and inhibiting microorganism respectively.
Sujet(s)
Crassostrea , Vitellogénines , Animaux , Vitellogénines/génétique , Vitellogénines/métabolisme , Staphylococcus aureus , Escherichia coli/métabolisme , Agglutination , ARN messager/génétique , Hémocytes , Immunité innée/génétiqueRÉSUMÉ
BACKGROUND: Fine-scale genetic structure of ethnolinguistically diverse Chinese populations can fill the gap in the missing diversity and evolutionary landscape of East Asians, particularly for anthropologically informed Chinese minorities. Hmong-Mien (HM) people were one of the most significant indigenous populations in South China and Southeast Asia, which were suggested to be the descendants of the ancient Yangtze rice farmers based on linguistic and archeological evidence. However, their deep population history and biological adaptative features remained to be fully characterized. OBJECTIVES: To explore the evolutionary and adaptive characteristics of the Miao people, we genotyped genome-wide SNP data in Guizhou HM-speaking populations and merged it with modern and ancient reference populations via a comprehensive population genetic analysis and evolutionary admixture modeling. RESULTS: The overall genetic admixture landscape of Guizhou Miao showed genetic differentiation between them and other linguistically diverse Guizhou populations. Admixture models further confirmed that Miao people derived their primary ancestry from geographically close Guangxi Gaohuahua people. The estimated identity by descent and effective population size confirmed a plausible population bottleneck, contributing to their unique genetic diversity and population structure patterns. We finally identified several natural selection candidate genes associated with several biological pathways. CONCLUSIONS: Guizhou Miao possessed a specific genetic structure and harbored a close genetic relationship with geographically close southern Chinese indigenous populations and Guangxi historical people. Miao people derived their major ancestry from geographically close Guangxi Gaohuahua people and experienced a plausible population bottleneck which contributed to the unique pattern of their genetic diversity and structure. Future ancient DNA from Shijiahe and Qujialing will provide new insights into the origin of the Miao people.
Sujet(s)
Adaptation biologique , Asiatiques , Humains , Haplotypes/génétique , Allèles , Chine , Asiatiques/génétiqueRÉSUMÉ
The IRF2BP family of transcription regulators act as corepressor molecules by inhibiting both enhancer-activated and basal transcription involving in many biological contexts. In the present study, an IRF2BP homologue (CgIRF2BP) was identified from oyster C. gigas. Its open reading frame is of 1809 bp encoding a polypeptide of 602 amino acids, which contains an IRF-2BP1_2 domain and a RING domain. The mRNA transcripts of CgIRF2BP were detected in all tested tissues with highest level in haemocytes (28.99-fold of that in mantle, p < 0.05). After poly (I:C) stimulation, the expression level of CgIRF2BP was significantly down-regulated at 3 h (0.50-fold of that in control group, p < 0.001) and gradually increased from 6 h to 48 h (2.69-fold of that in control group, p < 0.01). The recombinant protein of CgIRF2BP (rCgIRF2BP) showed high affinity to both rCgIRF1 and rCgIRF8 with Kd value of 1.02 × 10-7 and 2.09 × 10-7, respectively. In CgIRF2BP-RNAi oysters, the mRNA expression of CgIFNLP, CgMx1, CgViperin and CgIFI44L were significantly increased after poly (I:C) stimulation, which were 2.88 (p < 0.01), 1.83 (p < 0.05), 2.47 (p < 0.05), and 1.99-fold (p < 0.01) of that in EGFP group, respectively. These findings suggested that CgIRF2BP negatively regulated CgIFNLP expression by binding with CgIRF1 and CgIRF8.
Sujet(s)
Crassostrea , Immunité innée , Animaux , Immunité innée/génétique , Crassostrea/génétique , Régulation de l'expression des gènes , Protéines recombinantes/génétique , ARN messager/métabolisme , Hémocytes/métabolismeRÉSUMÉ
Interferon-stimulated genes (ISGs) encoding proteins are the essential executors of interferon (IFN) mediated antiviral defense. In the present study, an ISG member, interferon-induced protein 44-like (IFI44L) gene (designed as CgIFI44L-1) was identified from the Pacific oyster Crassostrea gigas. The ORF of CgIFI44L-1 cDNA was of 1437 bp encoding a polypeptide of 479 amino acids with a TLDc domain and an MMR_HSR1 domain. The mRNA transcripts of CgIFI44L-1 were detected in all the tested tissues with highest level in haemocytes, which was 15.78-fold of that in gonad (p < 0.001). Among the haemocytes, the CgIFI44L-1 protein was detected to be highly expressed in granulocytes with dominant distribution in cytoplasm. The mRNA expression level of CgIFI44L-1 in haemocytes was significantly induced by poly (I:C) stimulation, and the expression level peaked at 24 h, which was 24.24-fold (p < 0.0001) of that in control group. After the treatment with the recombinant protein of an oyster IFN-like protein (rCgIFNLP), the mRNA expression level of CgIFI44L-1 was significantly enhanced at 6 h, 12 h and 24 h, which was 2.67-fold (p < 0.001), 5.44-fold (p < 0.001) and 5.16-fold (p < 0.001) of that in control group, respectively. When the expressions of CgSTAT and CgIFNLP were knocked down by RNA interference (RNAi), the mRNA transcripts of CgIFI44L-1 were significantly down-regulated after poly (I:C) stimulation, which was 0.09-fold (p < 0.001) and 0.06-fold (p < 0.001) of those in EGFP group, respectively. These results suggested that CgIFI44L-1 was a conserved ISG in oyster, which was regulated by CgIFNLP and CgSTAT, and involved in the oyster antiviral immune response.
Sujet(s)
Crassostrea , Acides aminés/métabolisme , Animaux , Antiviraux/métabolisme , ADN complémentaire/métabolisme , Hémocytes , Immunité innée/génétique , Interférons/génétique , Interférons/métabolisme , Poly I-C/pharmacologie , ARN messager/métabolisme , Protéines recombinantes/génétiqueRÉSUMÉ
The stimulator of interferon gene (STING), an intracellular sensor of cyclic dinucleotides, is critical to the innate immune response, especially the induction of type I interferon (IFN) during pathogenic infection. A STING homologue (CgSTING) regulating the expression of IFN-like protein (CgIFNLP) was previously identified in the Pacific oyster Crassostrea gigas, and its involvement in antibacterial immunity was further investigated in the present study. The mRNA transcripts of CgSTING were ubiquitously detected in all the three subpopulations of haemocytes with the highest expression in semi-granulocytes. After the stimulation with Vibrio splendidus, the mRNA expression of CgSTING in haemocytes was significantly up-regulated and peaked at 72 h, which was 12.91-fold of that in control group (p < 0.01). The CgSTING protein was mainly located in the cytoplasm of haemocytes. After the expression of CgSTING was knocked down (0.12-fold of that in control group, p < 0.05) by RNAi, the mRNA expression levels of interleukin17-1 (CgIL17-1), interleukin17-3 (CgIL17-3), interleukin17-4 (CgIL17-4), defensins (Cgdefh1, Cgdefh2), big defensin (CgBigDef1), interferon-like protein (CgIFNLP), tumor necrosis factor (CgTNF) and nuclear factor-κB (CgRel) all decreased significantly at 12 h after V. splendidus stimulation, which was 0.12-fold-0.72-fold (p < 0.05) of that in control group, respectively. The positive signals of CgRel were observed in the haemocyte nucleus after V. splendidus stimulation. The nuclear translocation of CgRel was suppressed in CgSTING-RNAi oysters, and the green signals of CgRel were mainly observed in the haemocyte cytoplasm after V. splendidus stimulation. Furthermore, the number of V. splendidus in the haemolymph of CgSTING-RNAi oysters increased significantly, which was 26.78-fold (p < 0.01) of that in the control group at 12 h after V. splendidus stimulation. These results indicated that CgSTING played important role in the immune defense against bacterial infection by inducing the expressions of cytokines and defensins.
Sujet(s)
Anti-infectieux , Crassostrea , Interféron de type I , Animaux , Antibactériens/métabolisme , Anti-infectieux/métabolisme , Cytokines/métabolisme , Défensines , Hémocytes , Immunité innée/génétique , Interféron de type I/métabolisme , Facteur de transcription NF-kappa B/métabolisme , ARN messager/métabolisme , Facteur de nécrose tumorale alpha/métabolismeRÉSUMÉ
The RAC-alpha serine/threonine-protein kinase (AKT) is one of the most important protein kinases involved in many biological processes in eukaryotes. In the present study, a novel AKT homologue named CgAKT1 was identified from the Pacific oyster Crassostrea gigas. The open reading frame (ORF) of CgAKT1 cDNA was of 1482 bp encoding a peptide with 493 amino acid residues. There were classical domains in the predicted CgAKT1 protein, including an N-terminal pleckstrin homology domain, a central catalytic domain and a C-terminal hydrophobic domain. The mRNA transcripts of CgAKT1 were detected in all the examined tissues of C. gigas with higher level in gills (8.24-fold of that in mantle, p < 0.05) and haemocytes (3.62-fold of that in mantle, p < 0.05). After poly (I:C) stimulation, the mRNA expression of CgAKT1 decreased significantly in haemocytes from 3 h (0.44-fold of that in the control group, p < 0.001) to 24 h (0.20-fold of that in the control group, p < 0.001), and then increased significantly at 48 h (3.65-fold of that in the control group, p < 0.05). The expression level of CgAKT1 mRNA increased significantly at 6 h after rCgIFNLP stimulation, which was 3.60-fold of that in the control group (p < 0.001). The Alexa Fluor 488 positive signals of CgAKT1 protein were found to be distributed in the cytoplasm and cell membrane of haemocytes, while those in the cytoplasm became weaker after poly (I:C) stimulation. In CgAKT1-RNAi oysters, the mRNA expression of cyclic GMP-AMP synthase (CgcGAS) and TANK-binding kinase 1 (CgTBK1) did not change significantly, but the mRNA expression level of stimulator of interferon gene (CgSTING), interferon regulatory factor-1 (CgIRF-1), interferon regulatory factor-8 (CgIRF-8) and IFN-like protein (CgIFNLP) increased significantly, which was 1.40-fold, 1.53-fold, 1.72-fold and 1.99-fold of that in EGFP-RNAi oysters (p < 0.05), respectively. In CgIFNLP-RNAi oysters, the transcripts of CgAKT1 decreased significantly compared to those in EGFP-RNAi oysters (0.16-fold, p < 0.01). Moreover, the expression of p-CgTBK1, CgSTING and CgIFNLP at the protein level in the oysters treated with p-AKT1 activator (SC-79) was significantly suppressed after poly (I:C) stimulation. After the transfection of CgAKT1, the expression of p-cGAS protein in HEK293T cells increased significantly, while the cyclic GMP-AMP in the cells and the interferon (IFN-ß) in the cell culture fluid decreased significantly compared with that in the control group. These results indicated that CgAKT1 might play a negative role in antiviral immunity of oyster by regulating the synthesis of CgIFNLP.
Sujet(s)
Crassostrea , Animaux , Fluorescéines , Régulation de l'expression des gènes , Cellules HEK293 , Hémocytes , Humains , Immunité innée/génétique , Interférons/génétique , Poly I-C/pharmacologie , Protéines proto-oncogènes c-akt/génétique , Protéines proto-oncogènes c-akt/métabolisme , ARN messager/génétique , ARN messager/métabolisme , Sérine/génétique , Sérine/métabolisme , Acides sulfoniques , ThréonineRÉSUMÉ
The present study deals with the synthesis of zeolite-loaded FeOOH@ZnO by hydrothermal method and investigates the effects of coexisting SO32- and PO43- ions in the aqueous solution on the adsorption performance for S2-. The results showed that the HNO3-modified zeolite loaded with FeOOH@ZnO (FeOOH@ZnO/HZ) resulted in a maximum S2- removal rate of ≈98%. The adsorbent's performance on removing S2- was significantly enhanced, compared with NaOH and ZnCl2-modified zeolites loaded with FeOOH@ZnO, and the adsorption was proved to be a heat-absorbing process. When SO32- and PO43- coexisted with S2-, SO32- and PO43- had a significant influence on the adsorption properties of FeOOH@ZnO/HZ. When three ions of S2-, SO32- and PO43- were present simultaneously, the adsorption performance of FeOOH@ZnO/HZ on S2- was further, and the removal rate dropped to about 80%. Moreover, FeOOH@ZnO/HZ also adsorbed PO43- and SO32- in the system containing multiple ions, but the adsorption rates of PO43- and SO32- were much lower than S2-. This indicated that the adsorption of S2- in the presence of FeOOH@ZnO/HZ dominates under competitive conditions.
Sujet(s)
Zéolites , Oxyde de zinc , AdsorptionRÉSUMÉ
Metal sulfides with high activity are favorable electrode materials for supercapacitors. However, their relatively inferior electronic conductivity and poor stability in alkaline electrolyte solutions impede their applications. To overcome these drawbacks, herein, 2D/2D nanosheet heterostructures of Co3S4 and g-C3N4 have been successfully fabricated by a facile method that involves the in situ growth of 2D Co-based zeolitic imidazolate framework (Co-ZIF-L) crystals on g-C3N4 nanosheets followed by subsequent sulfurization. The as-prepared Co3S4/g-C3N4-10 exhibits a largely enhanced specific capacity (415.0 C g-1 at 0.5 A g-1) in comparison with solitary g-C3N4 (18.9 C g-1) and Co3S4 (194.4 C g-1) derived from Co-ZIF-L. Furthermore, it also displays good rate capability (54.5% retention at 10 A g-1). The asymmetric supercapacitor fabricated from Co3S4/g-C3N4-10 and activated carbon electrodes exhibits an outstanding energy density of 35.7 W h kg-1 at a high power density of 850.2 W kg-1. Most importantly, the asymmetric supercapacitor demonstrates an ultrahigh cycling durability with only 1.9% capacitance loss after 10 000 cycles at 10 A g-1. This superior electrochemical performance can be attributed to the unique 2D/2D nanosheet heterostructures providing rich active sites, short ion diffusion pathways, fast charge transfer as well as improved conductivity and mechanic stability. This work may pave the way for a rational design of the heterostructures of metal sulfides and g-C3N4 for electrochemical energy storage devices with a long cycling lifespan.
RÉSUMÉ
Structure and defect manipulation are regarded as efficacious strategies to boost the electrochemical activity of electrode materials. Herein, the construction of one-dimensional (1D) porous S-doped Co3O4 nanorods with rich oxygen vacancies is demonstrated through a facile metal-organic framework-engaged strategy. Starting from a Co-NTA (NTA = nitrilotriacetic acid) precursor, the S-doped Co3O4 nanorods were obtained after calcination and sulfurization. As a faradaic electrode material, the S-doped Co3O4 nanorods exhibited enhanced specific capacitance (319.3 C g-1 at 0.5 A g-1) in comparison with the Co3O4 intermediate product (98.3 C g-1) and the Co-NTA precursor (40.2 C g-1). Besides, it showed an ultra-high rate capability of 83.3% with a 20-fold increase in current density (10 A g-1). The hybrid supercapacitor comprising the S-doped Co3O4 (cathode) and the activated carbon (anode) showed a high energy density of 38.1 W h kg-1 at a power density of 800 W kg-1, and 31.1 W h kg-1 was maintained at 8000 W kg-1. It also has excellent electrochemical stability, and 87.57% of its initial capacitance was maintained after 5000 cycles, demonstrating great prospects in electrochemical energy storage applications. The excellent energy storage property of the S-doped Co3O4 is due to its unique 1D S-doped Co3O4 porous nanorod structure, i.e., large surface area, easy diffusion of ions, good conductivity, and rich redox reactions. This work may pave the way for the fabrication of desirable electrode materials through vacancy defects and nano-/microstructure engineering.