RÉSUMÉ
While significant progress has been achieved in utilizing remote sensing technologies for landslide investigation in China, there remains a notable gap in consolidating information on applicable conditions, application stages, and workflows across various remote sensing methodologies. This paper proposes a comprehensive framework for active landslide detection, incorporating multiple stages and data sources, successfully implemented in a vast region of southwestern China. Furthermore, detailed discussions are provided on the effects of the geometric distortion, land cover type, and various InSAR methods on the accuracy of active landslide identification results. Additionally, the paper delves into the advantages of integrated remote sensing technology in active landslide investigation, encompassing the assessment of current landslide activity status, precise delineation of boundaries, identification of different deformation stages, and determination of damage patterns. Through comprehensive analysis of multisource data, it enhances understanding of the active landslide process, ultimately contributing to the mitigation of casualties and property damage.
RÉSUMÉ
The emergence of drug-resistant viruses is a serious concern in current chemotherapy for human immunodeficiency virus type-1 (HIV-1) infectious diseases. Hence, antiviral drugs aiming at targets that are different from those of approved drugs are still required, and the RNase H activity of HIV-1 reverse transcriptase is a suitable target. In this study, a search of a series of natural compounds was performed to identify the RNase H inhibitors. Three compounds were found to block the RNase H enzymatic activity. A laccaic acid skeleton was observed in all three natural compounds. A hydroxy phenyl group is connected to an anthraquinone backbone in the skeleton. An acetamido-ethyl, amino-carboxy-ethyl, and amino-ethyl are bound to the phenyl in laccaic acids A, C, and E, respectively. Laccaic acid C showed a 50% inhibitory concentration at 8.1 µM. Laccaic acid C also showed inhibitory activity in a cell-based viral proliferation assay. Binding structures of these three laccaic acids were determined by X-ray crystallographic analysis using a recombinant protein composed of the HIV-1 RNase H domain. Two divalent metal ions were located at the catalytic center in which one carbonyl and two hydroxy groups on the anthraquinone backbone chelated two metal ions. Molecular dynamics simulations were performed to examine the stabilities of the binding structures. Laccaic acid C showed the strongest binding to the catalytic site. These findings will be helpful for the design of potent inhibitors with modification of laccaic acids to enhance the binding affinity.
Sujet(s)
Infections à VIH , Ribonuclease H , Humains , Ribonuclease H/métabolisme , Ions , Anthraquinones/pharmacologie , Inhibiteurs de la transcriptase inverse/pharmacologie , Inhibiteurs de la transcriptase inverse/composition chimiqueRÉSUMÉ
Chemotherapy of human immunodeficiency virus type-1 (HIV-1) has significantly developed over the last three decades. The emergence of drug-resistant variants is, however, still a severe problem. The RNase H activity of HIV-1 reverse transcriptase is an attractive target for a new class of antiviral drugs because there is no approved inhibitor. The nitro-furan-carbonyl and nitro-thiophene-carbonyl groups are potent scaffolds for the HIV-1 RNase H inhibitor. In this work, the binding structures of six inhibitory compounds were obtained by X-ray crystal analysis in a complex with a recombinant protein of HIV-1 RNase H domain. Every inhibitory compound was found to be bound to the catalytic site with the furan- or thiophene-ring coordinated to two divalent metal ions at the binding pocket. All the atoms in nitro, furan, carbonyl, and two metals were aligned in the nitro-furan derivatives. The straight line connecting nitro and carboxyl groups was parallel to the plane made by two metal ions and a furan O atom. The binding modes of the nitro-thiophene derivatives were slightly different from those of the nitro-furan ones. The nitro and carbonyl groups deviated from the plane made by two metals and a thiophene S atom. Molecular dynamics simulations suggested that the furan O or thiophene S atom and carbonyl O atom were firmly coordinated to the metal ions. The simulations made the planar nitro-furan moiety well aligned to the line connecting the two metal ions. In contrast, the nitro-thiophene derivatives were displaced from the initial positions after the simulations. The computational findings will be a sound basis for developing potent inhibitors for HIV-1 RNase H activity.
Sujet(s)
Agents antiVIH , VIH-1 (Virus de l'Immunodéficience Humaine de type 1) , Ribonuclease H , Humains , Domaine catalytique , Cristallographie aux rayons X , Furanes/pharmacologie , Furanes/composition chimique , Transcriptase inverse du VIH , VIH-1 (Virus de l'Immunodéficience Humaine de type 1)/effets des médicaments et des substances chimiques , VIH-1 (Virus de l'Immunodéficience Humaine de type 1)/enzymologie , Métaux/métabolisme , Inhibiteurs de la transcriptase inverse/pharmacologie , Inhibiteurs de la transcriptase inverse/composition chimique , Ribonuclease H/antagonistes et inhibiteurs , Agents antiVIH/composition chimique , Agents antiVIH/pharmacologieRÉSUMÉ
HIV-associated nephropathy (HIVAN) impairs functions of both glomeruli and tubules. Attention has been previously focused on the HIVAN glomerulopathy. Tubular injury has drawn increased attention because sodium wasting is common in hospitalized HIV/AIDS patients. We used viral protein R (Vpr)-transgenic mice to investigate the mechanisms whereby Vpr contributes to urinary sodium wasting. In phosphoenolpyruvate carboxykinase promoter-driven Vpr-transgenic mice, in situ hybridization showed that Vpr mRNA was expressed in all nephron segments, including the distal convoluted tubule. Vpr-transgenic mice, compared with wild-type littermates, markedly increased urinary sodium excretion, despite similar plasma renin activity and aldosterone levels. Kidneys from Vpr-transgenic mice also markedly reduced protein abundance of the Na+-Cl- cotransporter (NCC), while mineralocorticoid receptor (MR) protein expression level was unchanged. In African green monkey kidney cells, Vpr abrogated the aldosterone-mediated stimulation of MR transcriptional activity. Gene expression of Slc12a3 (NCC) in Vpr-transgenic mice was significantly lower compared with wild-type mice, assessed by both qRT-PCR and RNAScope in situ hybridization analysis. Chromatin immunoprecipitation assays identified multiple MR response elements (MRE), located from 5 kb upstream of the transcription start site and extending to the third exon of the SLC12A3 gene. Mutation of MRE and SP1 sites in the SLC12A3 promoter region abrogated the transcriptional responses to aldosterone and Vpr, indicating that functional MRE and SP1 are required for the SLC12A3 gene suppression in response to Vpr. Thus, Vpr attenuates MR transcriptional activity and inhibits Slc12a3 transcription in the distal convoluted tubule and contributes to salt wasting in Vpr-transgenic mice.
Sujet(s)
Produits du gène vpr , VIH-1 (Virus de l'Immunodéficience Humaine de type 1) , Aldostérone/métabolisme , Aldostérone/pharmacologie , Animaux , Chlorocebus aethiops , Produits du gène vpr/métabolisme , VIH-1 (Virus de l'Immunodéficience Humaine de type 1)/génétique , Tubules contournés distaux/métabolisme , Souris , Souris transgéniques , Phosphoénolpyruvate , ARN messager/métabolisme , Récepteurs des minéralocorticoïdes/génétique , Récepteurs des minéralocorticoïdes/métabolisme , Rénine/métabolisme , Sodium/métabolisme , Chlorure de sodium/métabolisme , Symporteurs des ions sodium-chlorure/métabolisme , Membre-3 de la famille-12 des transporteurs de solutés/génétique , Membre-3 de la famille-12 des transporteurs de solutés/métabolisme , ThiazidesRÉSUMÉ
Obesity is the major driver of the global epidemic in type 2 diabetes (T2D). In individuals with obesity, impaired insulin action leads to increased lipolysis in adipocytes, resulting in elevated plasma free fatty acid (FFA) levels that promote peripheral insulin resistance, a hallmark of T2D. Here we show, by using a combined genetic/biochemical/pharmacologic approach, that increased adipocyte lipolysis can be prevented by selective activation of adipocyte Gq signaling in vitro and in vivo (in mice). Activation of this pathway by a Gq-coupled designer receptor or by an agonist acting on an endogenous adipocyte Gq-coupled receptor (CysLT2 receptor) greatly improved glucose and lipid homeostasis in obese mice or in mice with adipocyte insulin receptor deficiency. Our findings identify adipocyte Gq signaling as an essential regulator of whole-body glucose and lipid homeostasis and should inform the development of novel classes of GPCR-based antidiabetic drugs.
Sujet(s)
Diabète de type 2 , Insulinorésistance , Adipocytes/métabolisme , Animaux , Diabète de type 2/génétique , Diabète de type 2/métabolisme , Glucose/métabolisme , Homéostasie , Lipides , Lipolyse , Souris , Souris de lignée C57BL , Souris obèse , Obésité/métabolismeRÉSUMÉ
The Repeat Expansion Diseases, a large group of human diseases that includes the fragile X-related disorders (FXDs) and Huntington's disease (HD), all result from expansion of a disease-specific microsatellite via a mechanism that is not fully understood. We have previously shown that mismatch repair (MMR) proteins are required for expansion in a mouse model of the FXDs, but that the FANCD2 and FANCI associated nuclease 1 (FAN1), a component of the Fanconi anemia (FA) DNA repair pathway, is protective. FAN1's nuclease activity has been reported to be dispensable for protection against expansion in an HD cell model. However, we show here that in a FXD mouse model a point mutation in the nuclease domain of FAN1 has the same effect on expansion as a null mutation. Furthermore, we show that FAN1 and another nuclease, EXO1, have an additive effect in protecting against MSH3-dependent expansions. Lastly, we show that the loss of FANCD2, a vital component of the Fanconi anemia DNA repair pathway, has no effect on expansions. Thus, FAN1 protects against MSH3-dependent expansions without diverting the expansion intermediates into the canonical FA pathway and this protection depends on FAN1 having an intact nuclease domain.
Sujet(s)
Domaine catalytique , Endodeoxyribonucleases/métabolisme , Exodeoxyribonucleases/métabolisme , Enzymes multifonctionnelles/métabolisme , Expansion de trinucléotide répété , Animaux , Enzymes de réparation de l'ADN/métabolisme , Endodeoxyribonucleases/composition chimique , Endodeoxyribonucleases/génétique , Exodeoxyribonucleases/composition chimique , Exodeoxyribonucleases/génétique , Protéine du groupe de complémentation D2 de l'anémie de Fanconi/génétique , Protéine du groupe de complémentation D2 de l'anémie de Fanconi/métabolisme , Souris , Souris de lignée C57BL , Enzymes multifonctionnelles/composition chimique , Enzymes multifonctionnelles/génétique , Protéine-3 homologue de MutS/métabolisme , Mutation ponctuelleRÉSUMÉ
PURPOSE: The goal of this study was to analyze the role of somatostatin receptor type 2 (SSTR2) as a molecular target for the imaging and treatment of thyroid cancer through analysis of SSTR2 expression and its epigenetic modulation and testing tumor uptake of different radiolabeled SSTR2 analogues. EXPERIMENTAL DESIGN: We analyzed SSTR2 expression by immunostaining of 92 thyroid cancer tissue samples and quantified standard uptake values (SUVmax) of SSTR2 analogue, 68Ga-DOTA-TATE, by PET/CT imaging in 25 patients with metastatic thyroid cancer. We utilized human thyroid cancer cell lines characterized by differential SSTR2 expression (TT, BCPAP, and FTC133) and rat pancreatic cell line (AR42J) with intrinsically high SSTR2 expression for functional in vitro studies. SSTR2-high (AR42J) and SSTR2-low (FTC133) xenograft mouse models were used to test the uptake of radiolabeled SSTR2 analogues and their therapeutic efficacy in vivo. RESULTS: Thyroid cancer had a higher SSTR2 expression than normal thyroid. Hurthle cell thyroid cancer was characterized by the highest 68Ga-DOTA-TATE uptake [median SUVmax, 16.5 (7.9-29)] than other types of thyroid cancers. In vivo studies demonstrated that radiolabeled DOTA-EB-TATE is characterized by significantly higher tumor uptake than DOTA-TATE (P < 0.001) and DOTA-JR11 (P < 0.001). Treatment with 177Lu-DOTA-EB-TATE extended survival and reduced tumor size in a mouse model characterized by high somatostatin (SST) analogues uptake (SUVmax, 15.16 ± 4.34), but had no effects in a model with low SST analogues uptake (SUVmax, 4.8 ± 0.27). CONCLUSIONS: A novel SST analogue, 177Lu-DOTA-EB-TATE, has the potential to be translated from bench to bedside for the targeted therapy of patients characterized by high uptake of SST analogues in metastatic lesions.
Sujet(s)
Radiopharmaceutiques/administration et posologie , Récepteur somatostatine/métabolisme , Somatostatine/analogues et dérivés , Tumeurs de la thyroïde/traitement médicamenteux , Adulte , Animaux , Apoptose , Prolifération cellulaire , Femelle , Humains , Souris , Souris de lignée NOD , Souris nude , Souris SCID , Adulte d'âge moyen , Métastase tumorale , Tomographie par émission de positons couplée à la tomodensitométrie , Pronostic , Radiopharmaceutiques/métabolisme , Récepteur somatostatine/composition chimique , Somatostatine/administration et posologie , Somatostatine/métabolisme , Tumeurs de la thyroïde/imagerie diagnostique , Tumeurs de la thyroïde/métabolisme , Tumeurs de la thyroïde/anatomopathologie , Cellules cancéreuses en culture , Tests d'activité antitumorale sur modèle de xénogreffeRÉSUMÉ
A better understanding of the signaling pathways regulating adipocyte function is required for the development of new classes of antidiabetic/obesity drugs. We here report that mice lacking ß-arrestin-1 (barr1), a cytoplasmic and nuclear signaling protein, selectively in adipocytes showed greatly impaired glucose tolerance and insulin sensitivity when consuming an obesogenic diet. In contrast, transgenic mice overexpressing barr1 in adipocytes were protected against the metabolic deficits caused by a high-calorie diet. Barr1 deficiency led to a myogenic reprogramming of brown adipose tissue (BAT), causing elevated plasma myostatin (Mstn) levels, which in turn led to impaired insulin signaling in multiple peripheral tissues. Additional in vivo studies indicated that barr1-mediated suppression of Mstn expression by BAT is required for maintaining euglycemia. These findings convincingly identify barr1 as a critical regulator of BAT function. Strategies aimed at enhancing barr1 activity in BAT may prove beneficial for the treatment of type 2 diabetes.
RÉSUMÉ
Analysis of individual gametes has a number of applications in the study of the mechanism of repeat expansion in mouse models of the fragile X-related disorders, as well as in mouse models of other repeat expansion diseases. This chapter describes the techniques required to isolate oocytes and male gametes of different stages of maturity, along with the techniques required to accurately determine the repeat number in these gametes.
Sujet(s)
Protéine du syndrome X fragile/génétique , Syndrome du chromosome X fragile/génétique , Ovocytes/cytologie , Spermatocytes/cytologie , Expansion de trinucléotide répété , Animaux , Séparation cellulaire , Modèles animaux de maladie humaine , Femelle , Cytométrie en flux , Humains , Mâle , Souris , Ovocytes/composition chimique , Analyse sur cellule unique , Spermatocytes/composition chimiqueRÉSUMÉ
Skeletal muscle (SKM) insulin resistance plays a central role in the pathogenesis of type 2 diabetes. Because G-protein-coupled receptors (GPCRs) represent excellent drug targets, we hypothesized that activation of specific functional classes of SKM GPCRs might lead to improved glucose homeostasis in type 2 diabetes. At present, little is known about the in vivo metabolic roles of the various distinct GPCR signaling pathways operative in SKM. In this study, we tested the hypothesis that selective activation of SKM Gq signaling can improve SKM glucose uptake and whole-body glucose homeostasis under physiological and pathophysiological conditions. Studies with transgenic mice expressing a Gq-linked designer GPCR selectively in SKM cells demonstrated that receptor-mediated activation of SKM Gq signaling greatly promoted glucose uptake into SKM and significantly improved glucose homeostasis in obese, glucose-intolerant mice. These beneficial metabolic effects required the activity of SKM AMPK. In contrast, obese mutant mice that lacked both Gαq and Gα11 selectively in SKM showed severe deficits in glucose homeostasis. Moreover, GPCR-mediated activation of Gq signaling also stimulated glucose uptake in primary human SKM cells. Taken together, these findings strongly suggest that agents capable of enhancing SKM Gq signaling may prove useful as novel antidiabetic drugs.
Sujet(s)
Sous-unités alpha Gq-G11 des protéines G/métabolisme , Intolérance au glucose/métabolisme , Glucose/métabolisme , Insulinorésistance , Muscles squelettiques/métabolisme , Obésité/métabolisme , Récepteurs couplés aux protéines G/métabolisme , Adenylate kinase/métabolisme , Animaux , Diabète de type 2/métabolisme , Humains , Souris , Souris obèse , Souris transgéniques , Myoblastes squelettiques , Transduction du signalRÉSUMÉ
AIMS: Signalling via Gq-coupled receptors is of profound importance in many cardiac diseases such as hypertrophy and arrhythmia. Nevertheless, owing to their widespread expression and the inability to selectively stimulate such receptors in vivo, their relevance for cardiac function is not well understood. We here use DREADD technology to understand the role of Gq-coupled signalling in vivo in cardiac function. METHODS AND RESULTS: We generated a novel transgenic mouse line that expresses a Gq-coupled DREADD (Dq) in striated muscle under the control of the muscle creatine kinase promotor. In vivo injection of the DREADD agonist clozapine-N-oxide (CNO) resulted in a dose-dependent, rapid mortality of the animals. In vivo electrocardiogram data revealed severe cardiac arrhythmias including lack of P waves, atrioventricular block, and ventricular tachycardia. Following Dq activation, electrophysiological malfunction of the heart could be recapitulated in the isolated heart ex vivo. Individual ventricular and atrial myocytes displayed a positive inotropic response and arrhythmogenic events in the absence of altered action potentials. Ventricular tissue sections revealed a strong co-localization of Dq with the principal cardiac connexin CX43. Western blot analysis with phosphor-specific antibodies revealed strong phosphorylation of a PKC-dependent CX43 phosphorylation site following CNO application in vivo. CONCLUSION: Activation of Gq-coupled signalling has a major impact on impulse generation, impulse propagation, and coordinated impulse delivery in the heart. Thus, Gq-coupled signalling does not only modulate the myocytes' Ca2+ handling but also directly alters the heart's electrophysiological properties such as intercellular communication. This study greatly advances our understanding of the plethora of modulatory influences of Gq signalling on the heart in vivo.
Sujet(s)
Potentiels d'action , Troubles du rythme cardiaque/métabolisme , Signalisation calcique , Sous-unités alpha Gq-G11 des protéines G/métabolisme , Rythme cardiaque , Myocarde/métabolisme , Récepteurs couplés aux protéines G/métabolisme , Animaux , Troubles du rythme cardiaque/génétique , Troubles du rythme cardiaque/physiopathologie , Clozapine/analogues et dérivés , Clozapine/pharmacologie , Connexine 43/métabolisme , MM Creatine Kinase/génétique , Sous-unités alpha Gq-G11 des protéines G/génétique , Préparation de coeur isolé , Mâle , Souris de lignée C57BL , Souris transgéniques , Phosphorylation , Régions promotrices (génétique) , Protéine kinase C/métabolisme , Récepteurs couplés aux protéines G/agonistes , Récepteurs couplés aux protéines G/génétiqueRÉSUMÉ
Preadipocytes initiate differentiation into adipocytes through a cascade of events. Mitotic clonal expansion, as one of the earliest events, is essential for adipogenesis. However, the underlying mechanisms that regulate mitotic clonal expansion remain elusive. SIRT6 is a member of the evolutionarily conserved sirtuin family of nicotinamide adenine dinucleotide (NAD)+-dependent protein deacetylases. Here, we show that SIRT6 deficiency in preadipocytes blocks their adipogenesis. Analysis of gene expression during adipogenesis reveals that KIF5C, which belongs to the kinesin family, is negatively regulated by SIRT6. Furthermore, we show that KIF5C is a negative factor for adipogenesis through interacting with CK2α', a catalytic subunit of CK2. This interaction blocks CK2α' nuclear translocation and CK2 kinase activity and inhibits mitotic clonal expansion during adipogenesis. These findings reveal a crucial role of SIRT6 in adipogenesis and provide potential therapeutic targets for obesity.
Sujet(s)
Adipocytes/cytologie , Adipocytes/métabolisme , Différenciation cellulaire , Mitose , Sirtuines/métabolisme , Cellules 3T3-L1 , Adipogenèse , Animaux , Casein Kinase II/métabolisme , Noyau de la cellule/métabolisme , Prolifération cellulaire , Clones cellulaires , Délétion de gène , Kinésine/métabolisme , Souris , Phénotype , Transport des protéines , Sirtuines/déficitRÉSUMÉ
ß-arrestins are critical signalling molecules that regulate many fundamental physiological functions including the maintenance of euglycemia and peripheral insulin sensitivity. Here we show that inactivation of the ß-arrestin-2 gene, barr2, in ß-cells of adult mice greatly impairs insulin release and glucose tolerance in mice fed with a calorie-rich diet. Both glucose and KCl-induced insulin secretion and calcium responses were profoundly reduced in ß-arrestin-2 (barr2) deficient ß-cells. In human ß-cells, barr2 knockdown abolished glucose-induced insulin secretion. We also show that the presence of barr2 is essential for proper CAMKII function in ß-cells. Importantly, overexpression of barr2 in ß-cells greatly ameliorates the metabolic deficits displayed by mice consuming a high-fat diet. Thus, our data identify barr2 as an important regulator of ß-cell function, which may serve as a new target to improve ß-cell function.
Sujet(s)
Cellules à insuline/métabolisme , Ilots pancréatiques/métabolisme , Transduction du signal/génétique , bêta-Arrestine 2/génétique , Animaux , Calcium-Calmodulin-Dependent Protein Kinase Type 2/métabolisme , Lignée cellulaire , Lignée cellulaire tumorale , Alimentation riche en graisse , Expression des gènes , Humains , Insuline/métabolisme , Sécrétion d'insuline , Souris de lignée C57BL , Souris knockout , Souris transgéniques , bêta-Arrestine 2/métabolismeRÉSUMÉ
G protein-coupled receptors (GPCRs) regulate virtually all physiological functions including the release of insulin from pancreatic ß-cells. ß-Cell M3 muscarinic receptors (M3Rs) are known to play an essential role in facilitating insulin release and maintaining proper whole-body glucose homeostasis. As is the case with other GPCRs, M3R activity is regulated by phosphorylation by various kinases, including GPCR kinases and casein kinase 2 (CK2). At present, it remains unknown which of these various kinases are physiologically relevant for the regulation of ß-cell activity. In the present study, we demonstrate that inhibition of CK2 in pancreatic ß-cells, knockdown of CK2α expression, or genetic deletion of CK2α in ß-cells of mutant mice selectively augmented M3R-stimulated insulin release in vitro and in vivo. In vitro studies showed that this effect was associated with an M3R-mediated increase in intracellular calcium levels. Treatment of mouse pancreatic islets with CX4945, a highly selective CK2 inhibitor, greatly reduced agonist-induced phosphorylation of ß-cell M3Rs, indicative of CK2-mediated M3R phosphorylation. We also showed that inhibition of CK2 greatly enhanced M3R-stimulated insulin secretion in human islets. Finally, CX4945 treatment protected mice against diet-induced hyperglycemia and glucose intolerance in an M3R-dependent fashion. Our data demonstrate, for the first time to our knowledge, the physiological relevance of CK2 phosphorylation of a GPCR and suggest the novel concept that kinases acting on ß-cell GPCRs may represent novel therapeutic targets.
Sujet(s)
Casein Kinase II/physiologie , Insuline/métabolisme , Récepteur muscarinique de type M3/physiologie , Animaux , Cellules COS , Chlorocebus aethiops , Femelle , Cellules HEK293 , Humains , Sécrétion d'insuline , Mâle , Souris , Souris de lignée C57BL , Naphtyridines/pharmacologie , PhénazinesRÉSUMÉ
Increased hepatic glucose production is a key pathophysiological feature of type 2 diabetes. Like all other cell types, hepatocytes express many G protein-coupled receptors (GPCRs) that are linked to different functional classes of heterotrimeric G proteins. The important physiological functions mediated by G(s)-coupled hepatic glucagon receptors are well-documented. In contrast, little is known about the in vivo physiological roles of hepatocyte GPCRs that are linked to G proteins of the G(q) family. To address this issue, we established a transgenic mouse line (Hep-Rq mice) that expressed a G(q)-linked designer receptor (Rq) in a hepatocyte-selective fashion. Importantly, Rq could no longer bind endogenous ligands but could be selectively activated by a synthetic drug, clozapine-N-oxide. Clozapine-N-oxide treatment of Hep-Rq mice enabled us to determine the metabolic consequences caused by selective activation of a G(q)-coupled GPCR in hepatocytes in vivo. We found that acute Rq activation in vivo led to pronounced increases in blood glucose levels, resulting from increased rates of glycogen breakdown and gluconeogenesis. We also demonstrated that the expression of the V(1b) vasopressin receptor, a G(q)-coupled receptor expressed by hepatocytes, was drastically increased in livers of ob/ob mice, a mouse model of diabetes. Strikingly, treatment of ob/ob mice with a selective V(1b) receptor antagonist led to reduced glucose excursions in a pyruvate challenge test. Taken together, these findings underscore the importance of G(q)-coupled receptors in regulating hepatic glucose fluxes and suggest novel receptor targets for the treatment of type 2 diabetes.
Sujet(s)
Diabète de type 2/induit chimiquement , Activateurs d'enzymes/effets indésirables , Kinases associées à des récepteurs couplés à une protéine G/métabolisme , Sous-unités alpha Gq-G11 des protéines G/métabolisme , Néoglucogenèse/effets des médicaments et des substances chimiques , Glycogénolyse/effets des médicaments et des substances chimiques , Hépatocytes/effets des médicaments et des substances chimiques , Animaux , Antagonistes des récepteurs de l'hormone antidiurétique , Cellules cultivées , Diabète de type 2/traitement médicamenteux , Diabète de type 2/métabolisme , Activateurs d'enzymes/pharmacologie , Femelle , Kinases associées à des récepteurs couplés à une protéine G/composition chimique , Kinases associées à des récepteurs couplés à une protéine G/génétique , Sous-unités alpha Gq-G11 des protéines G/antagonistes et inhibiteurs , Hépatocytes/cytologie , Hépatocytes/métabolisme , Humains , Hypoglycémiants/usage thérapeutique , Mâle , Souris , Souris obèse , Souris transgéniques , Ingénierie des protéines , Motifs et domaines d'intéraction protéique , Récepteur muscarinique de type M3/agonistes , Récepteur muscarinique de type M3/composition chimique , Récepteur muscarinique de type M3/génétique , Récepteur muscarinique de type M3/métabolisme , Récepteurs à la vasopressine/métabolisme , Protéines de fusion recombinantes/composition chimique , Protéines de fusion recombinantes/métabolisme , Organismes exempts d'organismes pathogènes spécifiquesRÉSUMÉ
Type 2 diabetes (T2D) has emerged as a major threat to human health in most parts of the world. Therapeutic strategies aimed at improving pancreatic ß cell function are predicted to prove beneficial for the treatment of T2D. In the present study, we demonstrate that drug-mediated, chronic, and selective activation of ß cell G(q) signaling greatly improve ß cell function and glucose homeostasis in mice. These beneficial metabolic effects were accompanied by the enhanced expression of many genes critical for ß cell function, maintenance, and differentiation. By employing a combination of in vivo and in vitro approaches, we identified a novel ß cell pathway through which receptor-activated G(q) leads to the sequential activation of ERK1/2 and IRS2 signaling, thus triggering a series of events that greatly improve ß cell function. Importantly, we found that chronic stimulation of a designer G(q)-coupled receptor selectively expressed in ß cells prevented both streptozotocin-induced diabetes and the metabolic deficits associated with the consumption of a high-fat diet in mice. Since ß cells are endowed with numerous receptors that mediate their cellular effects via activation of G(q)-type G proteins, our findings provide a rational basis for the development of novel antidiabetic drugs targeting this class of receptors.
Sujet(s)
Clozapine/analogues et dérivés , Sous-unités alpha Gq-G11 des protéines G/métabolisme , Hypoglycémiants/pharmacologie , Cellules à insuline/effets des médicaments et des substances chimiques , Récepteurs couplés aux protéines G/agonistes , Animaux , Lignée cellulaire tumorale , Prolifération cellulaire , Clozapine/pharmacologie , Diabète expérimental/anatomopathologie , Diabète expérimental/prévention et contrôle , Évaluation préclinique de médicament , Femelle , Sous-unités alpha Gq-G11 des protéines G/génétique , Expression des gènes/effets des médicaments et des substances chimiques , Substrats du récepteur à l'insuline/génétique , Substrats du récepteur à l'insuline/métabolisme , Substrats du récepteur à l'insuline/physiologie , Cellules à insuline/métabolisme , Cellules à insuline/physiologie , Système de signalisation des MAP kinases , Mâle , Souris , Souris de lignée C57BL , Souris transgéniques , Thérapie moléculaire ciblée , Agonistes muscariniques/pharmacologie , Ingénierie des protéines , Récepteur muscarinique de type M3/agonistes , Récepteur muscarinique de type M3/physiologie , Récepteurs couplés aux protéines G/génétique , Récepteurs couplés aux protéines G/métabolisme , Protéines recombinantes/agonistes , Protéines recombinantes/génétique , Protéines recombinantes/métabolismeRÉSUMÉ
Ghrelin is a gastric peptide hormone that controls appetite and energy homeostasis. Plasma ghrelin levels rise before a meal and fall quickly thereafter. Elucidation of the regulation of ghrelin secretion has been hampered by the difficulty of directly interrogating ghrelin cells diffusely scattered within the complex gastric mucosa. Therefore, we generated transgenic mice with ghrelin cell expression of green fluorescent protein (GFP) to enable characterization of ghrelin secretion in a pure population of isolated gastric ghrelin-expressing GFP (Ghr-GFP) cells. Using quantitative RT-PCR and immunofluorescence staining, we detected a high level of expression of the long-chain fatty acid (LCFA) receptor GPR120, while the other LCFA receptor, GPR40, was undetectable. In short-term-cultured pure Ghr-GFP cells, the LCFAs docosadienoic acid, linolenic acid, and palmitoleic acid significantly suppressed ghrelin secretion. The physiological mechanism of LCFA inhibition on ghrelin secretion was studied in mice. Serum ghrelin levels were transiently suppressed after gastric gavage of LCFA-rich lipid in mice with pylorus ligation, indicating that the ghrelin cell may directly sense increased gastric LCFA derived from ingested intraluminal lipids. Meal-induced increase in gastric mucosal LCFA was assessed by measuring the transcripts of markers for tissue uptake of LCFA, lipoprotein lipase (LPL), fatty acid translocase (CD36), glycosylphosphatidylinositol-anchored HDL-binding protein 1, and nuclear fatty acid receptor peroxisome proliferator-activated receptor-γ. Quantitative RT-PCR studies indicate significantly increased mRNA levels of lipoprotein lipase, glycosylphosphatidylinositol-anchored HDL-binding protein 1, and peroxisome proliferator-activated receptor-γ in postprandial gastric mucosa. These results suggest that meal-related increases in gastric mucosal LCFA interact with GPR120 on ghrelin cells to inhibit ghrelin secretion.
Sujet(s)
Ghréline/métabolisme , Récepteurs couplés aux protéines G/physiologie , Animaux , Cellules cultivées , Acides gras/pharmacologie , Muqueuse gastrique/métabolisme , Souris , Souris transgéniques , Période post-prandiale , ARN messager/métabolismeRÉSUMÉ
CD44(+)/CD24(-) cells have been associated with breast cancer stem/progenitor cell features. However, the status of this phenotype cells in normal, benign and malignant breast tissues has not been studied, and the clinical correlation of this subpopulation in breast cancer is not fully understood. The present study sought to identify these cells in a series of normal, benign, and malignant breast tissues and explore their correlation to the molecular subtypes of breast carcinoma and conventional pathological features. Double-staining immunohistochemistry (DIHC) of CD44 and CD24 was performed on 30 normal breast tissues, 30 breast fibroadenomas (FA), 60 breast invasive ductal carcinomas (IDC), and 3 breast cancer cell lines (MCF-7, MDA-MB-468, and MDA-MB-231). In the normal breast tissues and FAs, three phenotypes were observed including CD44(+)/CD24(+), CD44(+)/CD24(-), and CD44(-)/CD24(-) cells. In the IDCs, CD44(-)/CD24(+) cells were detected, in addition to the three aforementioned phenotypes. The strong positive rate (+++, incidence >60%) of CD44(+)/CD24(-) was significantly increased from normal breast tissue, FAs to IDCs (0.0%-->6.7%-->21.7%). However, the CD44(+)/CD24(-) cells didn't correlate with ages of patients, lymph node metastasis, tumor size, molecular subtypes, and the expression of ER, PR, HER-2, PS2, Bcl-2, nm23. The proportion of CD44(+)/CD24(-) cells in MCF-7, MDA-MB-468, and MDA-MB-231 was about 1, 5, and 80%, respectively. The results indicate that the CD44(+)/CD24(-) cells are transit progenitors and have no association with the molecular subtypes and clinicopathological parameters in the IDCs.
Sujet(s)
Tumeurs du sein/immunologie , Antigènes CD24/métabolisme , Carcinome canalaire du sein/immunologie , Fibroadénome/immunologie , Antigènes CD44/métabolisme , Cellules souches tumorales/immunologie , Marqueurs biologiques tumoraux/métabolisme , Tumeurs du sein/anatomopathologie , Carcinome canalaire du sein/anatomopathologie , Lignée cellulaire tumorale , Femelle , Fibroadénome/anatomopathologie , Humains , Immunohistochimie , Invasion tumorale , Stadification tumorale , Cellules souches tumorales/anatomopathologie , PhénotypeRÉSUMÉ
Previous studies have shown that ß-cell M(3) muscarinic acetylcholine receptors (M3Rs) play a key role in maintaining blood glucose homeostasis by enhancing glucose-dependent insulin release. In this study, we tested the hypothesis that long-term, persistent activation of ß-cell M3Rs can improve glucose tolerance and ameliorate the metabolic deficits associated with the consumption of a high-fat diet. To achieve the selective and persistent activation of ß-cell M3Rs in vivo, we generated transgenic mice that expressed the Q490L mutant M3R in their pancreatic ß-cells (ß-M3-Q490L Tg mice). The Q490L point mutation is known to render the M3R constitutively active. The metabolic phenotypes of the transgenic mice were examined in several in vitro and in vivo metabolic tests. In the presence of 15 mm glucose and the absence of M3R ligands, isolated perifused islets prepared from ß-M3-Q490L Tg mice released considerably more insulin than wild-type control islets. This effect could be completely blocked by incubation of the transgenic islets with atropine (10 µm), an inverse muscarinic agonist, indicating that the Q490L mutant M3R exhibited ligand-independent signaling (constitutive activity) in mouse ß-cells. In vivo studies showed that ß-M3-Q490L Tg mice displayed greatly improved glucose tolerance and increased serum insulin levels as well as resistance to diet-induced glucose intolerance and hyperglycemia. These results suggest that chronic activation of ß-cell M3Rs may represent a useful approach to boost insulin output in the long-term treatment of type 2 diabetes.