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Nat Commun ; 9(1): 1513, 2018 04 17.
Article de Anglais | MEDLINE | ID: mdl-29666415

RÉSUMÉ

Chagas disease is caused by infection with the protozoan Trypanosoma cruzi (T. cruzi) and is an important cause of severe inflammatory heart disease. However, the mechanisms driving Chagas disease cardiomyopathy have not been completely elucidated. Here, we show that the canonical PI3Kγ pathway is upregulated in both human chagasic hearts and hearts of acutely infected mice. PI3Kγ-deficient mice and mutant mice carrying catalytically inactive PI3Kγ are more susceptible to T. cruzi infection. The canonical PI3Kγ signaling in myeloid cells is essential to restrict T. cruzi heart parasitism and ultimately to avoid myocarditis, heart damage, and death of mice. Furthermore, high PIK3CG expression correlates with low parasitism in human Chagas' hearts. In conclusion, these results indicate an essential role of the canonical PI3Kγ signaling pathway in the control of T. cruzi infection, providing further insight into the molecular mechanisms involved in the pathophysiology of chagasic heart disease.


Sujet(s)
Cardiomyopathie associée à la maladie de Chagas/immunologie , Phosphatidylinositol 3-kinases de classe Ib/métabolisme , Transduction du signal/immunologie , Trypanosoma cruzi/immunologie , Adulte , Animaux , Biopsie , Lignée cellulaire , Cardiomyopathie associée à la maladie de Chagas/parasitologie , Cardiomyopathie associée à la maladie de Chagas/anatomopathologie , Phosphatidylinositol 3-kinases de classe Ib/génétique , Modèles animaux de maladie humaine , Femelle , Coeur/parasitologie , Humains , Mâle , Souris , Souris de lignée C57BL , Souris knockout , Adulte d'âge moyen , Cellules myéloïdes/immunologie , Cellules myéloïdes/métabolisme , Myocarde/immunologie , Myocarde/anatomopathologie , Inhibiteurs des phosphoinositide-3 kinases , Quinoxalines/pharmacologie , Thiazolidinediones/pharmacologie , Trypanosoma cruzi/pathogénicité , Régulation positive
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