RÉSUMÉ
Primary infiltrating ductal carcinomas (IDCs) of the breast which measure less than 0.5 cm (T1a lesions) and between 0.5 and 1.0 cm (T1b lesions) are associated with a small risk of nodal metastasis. The role of axillary dissection in T1a and T1b breast cancer is controversial. In the absence of axillary dissection, comparable prognostic information might be obtained by examination of the primary cancer. The adhesion molecule CD44 represents a family of transmembrane proteins that mediate cell-cell and cell-matrix interactions. Previous investigators have correlated expression of CD44 and its isoforms with prognosis in breast cancer. We investigated the value of CD44 isoform expression as a predictor of nodal metastases in nonpalpable T1a and T1b IDC. Monoclonal antibody against the standard form of CD44 (CD44s) and polyclonal antibody directed against the variant isoform (CD44v6) was tested on 34 cases of nonpalpable node-negative infiltrating ductal carcinoma (IDC) less than 1.0 cm and 9 cases of nonpalpable node-positive IDC less than 1.0 cm. The expression of CD44s was significantly decreased in node-positive T1a and T1b IDC versus node-negative T1a and T1b IDC (11% vs 65%). In contrast, 97% of the node-negative IDC and 100% of the node-positive IDC expressed the CD44v6 isoform. We conclude that CD44s expression is significantly altered in T1a and T1b IDC with nodal metastases but that the CD44v6 isoform does not correlate with nodal metastases in nonpalpable stage T1a and T1b IDC.
Sujet(s)
Antigènes néoplasiques/métabolisme , Tumeurs du sein/métabolisme , Carcinome canalaire du sein/métabolisme , Antigènes CD44/métabolisme , Tumeurs du sein/anatomopathologie , Carcinome canalaire du sein/anatomopathologie , Femelle , Humains , Immunohistochimie , Métastase lymphatique , Pronostic , Valeurs de référenceRÉSUMÉ
BACKGROUND: Malignant transformation to endometriosis is a well documented phenomenon that occurs most commonly in the ovaries with cancer arising in extra-ovarian endometriosis being a rare event. METHODS: A retrospective clinical-pathological evaluation of eleven cases with malignant tumors arising in endometriosis was performed to evaluate the prognostic impact of various factors. Nuclear DNA content (ploidy) was assessed through flow cytometric study. RESULTS: Ovarian origin was identified in eight cases and three were associated with extra-ovarian endometriosis. Histologic type was endometrioid carcinoma in ten patients. The eleventh case had high grade endometrial stromal sarcoma. All tumors were diploid with no relation to stage, grade, or clinical outcome. The S-phase fraction (SPF) was analyzed in nine patients and no correlation could be demonstrated with any histologic parameters or clinical outcome. CONCLUSIONS: The DNA content seems to have no association with the classical prognostic parameters in these cases.
Sujet(s)
Endométriose/anatomopathologie , Tumeurs de l'appareil génital féminin/anatomopathologie , Adulte , Sujet âgé , Carcinome endométrioïde/étiologie , Carcinome endométrioïde/anatomopathologie , Transformation cellulaire néoplasique , ADN/analyse , Tumeurs de l'endomètre/étiologie , Tumeurs de l'endomètre/anatomopathologie , Endométriose/complications , Endométriose/chirurgie , Femelle , Cytométrie en flux , Tumeurs de l'appareil génital féminin/étiologie , Humains , Adulte d'âge moyen , Tumeurs de l'ovaire/étiologie , Tumeurs de l'ovaire/anatomopathologie , Tumeurs du bassin/étiologie , Tumeurs du bassin/anatomopathologie , Tumeurs du bassin/thérapie , Ploïdies , Pronostic , Études rétrospectives , Sarcomes/étiologie , Sarcomes/anatomopathologie , Tumeurs du vagin/étiologie , Tumeurs du vagin/anatomopathologie , Tumeurs du vagin/chirurgieRÉSUMÉ
Loss of heterozygosity (LOH) from the short arm of chromosome 8 (8p) is frequent in many human cancers, including breast, colon, prostate, and bladder cancers. LOH occurs in two regions of 8p, 8p21 and 8p22, and suggests the presence of two separate tumor suppressor genes. In breast cancers, 8p LOH occurs in both early and late clinical stage tumors, while in colon, prostate, and bladder cancers, there is an association between 8p LOH and advanced clinical stage. We investigated this discrepancy by comparing 8p LOH in infiltrating ductal carcinomas (IDC) to breast cancers of earlier clinical stage, i.e., tumors with no invasion [ductal carcinoma in situ (DCIS)-only tumors]. We used three markers which sample several reported loci of 8p LOH. We microdissected tumor from paraffin blocks of 39 IDC and 23 DCIS-only breast cancers and amplified tumor/normal DNA pairs for the microsatellite markers D8S254 (8p22), D8S133 (8p21.3), and NEFL (8p21). All cases of IDC were informative with at least one marker, with a combined rate of LOH of 46%. The results for each marker were [no. LOH/no. informative (%)]: D8S254, 8/26 (31%); D8S133 12/31 (39%), and NEFL, 9/25 (36%). In the DCIS-only group, all 23 were informative for at least one marker, but 8p LOH was absent. We conclude that 8p LOH from 8p21-22 is frequent in IDC of the breast, but absent in DCIS-only cases, and may play a role in breast cancer progression by conferring invasive ability.
Sujet(s)
Tumeurs du sein/génétique , Carcinome canalaire du sein/génétique , Délétion de segment de chromosome , Chromosomes humains de la paire 8 , Tumeurs du sein/anatomopathologie , Carcinome canalaire du sein/anatomopathologie , ADN tumoral , Femelle , Marqueurs génétiques , Humains , Répétitions microsatellites , Invasion tumorale , Réaction de polymérisation en chaîne , Polymorphisme génétiqueRÉSUMÉ
We report the fourth case of chondromyxoid fibroma of the sternum described in the literature. The clinical, radiographic, pathologic and treatment aspects of this case are presented, and the literature on chondromyxoid fibroma in this unusual location is reviewed.
Sujet(s)
Chondroblastome , Sternum , Tumeurs du thorax , Adulte , Chondroblastome/imagerie diagnostique , Chondroblastome/anatomopathologie , Chondroblastome/chirurgie , Femelle , Humains , Radiographie , Sternum/imagerie diagnostique , Sternum/anatomopathologie , Sternum/chirurgie , Tumeurs du thorax/imagerie diagnostique , Tumeurs du thorax/anatomopathologie , Tumeurs du thorax/chirurgieRÉSUMÉ
Opportunistic mycotic infections, such as aspergillosis, can produce morbid consequences with or without aggressive therapy in an immunocompromised patient. Treatment including amphotericin B and resection of the infected tissue must be considered early in the overall management of the patient. We describe two patients with acute myelogenous leukemia who underwent intense cytoreductive therapy with bone marrow transplantation and an associated fungal infection treated with an investigational form of amphotericin B.
Sujet(s)
Aspergillose/étiologie , Sujet immunodéprimé , Leucémie aigüe myéloïde/complications , Sinusite/microbiologie , Stomatite/microbiologie , Adulte , Amphotéricine B/usage thérapeutique , Protocoles de polychimiothérapie antinéoplasique/effets indésirables , Aspergillose/imagerie diagnostique , Aspergillose/traitement médicamenteux , Transplantation de moelle osseuse/effets indésirables , Femelle , Humains , Leucémie aigüe myéloïde/thérapie , Mâle , Fistule buccosinusienne/étiologie , Sinusite/traitement médicamenteux , Sinusite/étiologie , Stomatite/traitement médicamenteux , Stomatite/étiologie , TomodensitométrieRÉSUMÉ
We report a case of bilaterally multifocal renal oncocytomas in a patient with chronic renal disease and a long history of hypertension. Histologic study of the multiple oncocytic neoplasms with hematoxylin-eosin, periodic acid-Schiff, trichrome, and colloidal iron stains revealed novel, intracytoplasmic lumina, which were accentuated by immunoperoxidase staining with high-molecular-weight cytokeratin, epithelial membrane antigen, and Arachis hypogaea lectin. Ultrastructural studies demonstrated multiple microvilli lining the intracytoplasmic lumina. We also review the literature on bilaterally multifocal oncocytomas.