RÉSUMÉ
Waterpipe tobacco smoking is increasing in popularity, particularly among young adults. This popularity is related to the lack knowledge regarding the health effects of waterpipe smoking. In this study, we examined the genotoxicity of waterpipe smoking using an 8-hydroxy deoxyguanosine (8-OHdG) assay. Genotoxicity was evaluated in the saliva, urine, and serum of 66 waterpipe adult smokers and 46 healthy nonsmokers. The level of addiction to waterpipe smoking was evaluated using the Lebanon Waterpipe Dependence Scale-11. Levels of 8-OHdG in the samples were measured using commercially available enzyme-linked immunosorbent assays. Levels of 8-OHdG in the saliva (52,430 ± 2923 vs 48,430 ± 4189 pg/mL), urine (2815 ± 312 vs 2608 ± 180 pg/mL), and serum (19,720 ± 202 vs 19,670 ± 254 pg/mL) were similar between waterpipe smokers and nonsmokers (P > 0.05). In addition, no correlations were found between dependence score and levels of 8-OHdG in all sample types. In conclusion, 8-OHdG is not a good biomarker for genotoxic effect of waterpipe smoking.
Sujet(s)
Désoxyguanosine/analogues et dérivés , Mutagènes/toxicité , Nicotiana/composition chimique , Nicotiana/toxicité , Fumer/effets indésirables , 8-Hydroxy-2'-désoxyguanosine , Adolescent , Adulte , Marqueurs biologiques , Études cas-témoins , Enfant , Études transversales , Désoxyguanosine/sang , Désoxyguanosine/métabolisme , Désoxyguanosine/urine , Test ELISA , Femelle , Humains , Mâle , Facteurs socioéconomiques , Jeune adulteRÉSUMÉ
The present investigation aimed to determine the effects of age and seasons of the year on the testicular sperm reserve and seru m testosterone concentration in dromedary camels. Testes were collected from a local slaughterhouse during a period of one year ( breeding and non - breeding seasons) from two age groups (GI: 4 - 6 y r and GII: 8 - 10 y r ). Testicular sperm reserve was determined during the breeding ( December - May ) and non - breeding ( June - November ) seasons. Blood samples were collected for determination of serum testosterone concentration (ng/ml) . There was highly significant difference (P < 0.01) in the testicular sperm reserve between breeding (1 3 . 5 8 x 10 9 ) and non - breeding ( 9 . 90 x 10 9 ) seasons. The serum testosterone concentration (ng/ml) w as significantly increased during the breeding season (10.94 ng/ml) compared with that of the non - breeding season ( 4.43 ng/ml) . Testicular sperm reserve (1 4 . 3 4 in GII vs . 9 . 13 in GI) and serum testosterone concentration (10.43 vs . 4.94 ng /ml ) were significantly (P < 0.01) increased by age . It co uld be concluded that the age of the animal and season of the year have significant effects on the testicular sperm reserve and serum testosterone concentration in dromedary camels.
Sujet(s)
Animaux , Sperme , Testostérone/composition chimique , Testicule/anatomie et histologie , Chameaux/classificationRÉSUMÉ
The present investigation aimed to determine the effects of age and seasons of the year on the testicular sperm reserve and seru m testosterone concentration in dromedary camels. Testes were collected from a local slaughterhouse during a period of one year ( breeding and non - breeding seasons) from two age groups (GI: 4 - 6 y r and GII: 8 - 10 y r ). Testicular sperm reserve was determined during the breeding ( December - May ) and non - breeding ( June - November ) seasons. Blood samples were collected for determination of serum testosterone concentration (ng/ml) . There was highly significant difference (P < 0.01) in the testicular sperm reserve between breeding (1 3 . 5 8 x 10 9 ) and non - breeding ( 9 . 90 x 10 9 ) seasons. The serum testosterone concentration (ng/ml) w as significantly increased during the breeding season (10.94 ng/ml) compared with that of the non - breeding season ( 4.43 ng/ml) . Testicular sperm reserve (1 4 . 3 4 in GII vs . 9 . 13 in GI) and serum testosterone concentration (10.43 vs . 4.94 ng /ml ) were significantly (P < 0.01) increased by age . It co uld be concluded that the age of the animal and season of the year have significant effects on the testicular sperm reserve and serum testosterone concentration in dromedary camels.(AU)