RÉSUMÉ
Early embryonic development in Xenopus is characterized by transcriptional repression which is relieved at the mid-blastula stage. Here we show that most of the maternally inherited POU domain transcription factor Oct-1 is retained in the cytoplasm during early development, and that it gradually translocates to the nucleus around the mid-blastula transition. Overexpressed epitope-tagged Oct-1 exhibits highly similar localization properties compared to endogenous protein. The amino acid sequence that directs this developmentally regulated nuclear translocation resides in the POU domain. Our findings may suggest that cytoplasmic retention of Oct-1 facilitates or contributes to the repression of Oct-1 target genes before the mid-blastula transition.
Sujet(s)
Protéines de liaison à l'ADN/métabolisme , Facteurs de transcription/métabolisme , Animaux , Transport biologique , Noyau de la cellule/métabolisme , Protéines de liaison à l'ADN/génétique , Facteur de prolifération cellulaire HCF , Facteur de transcription Oct-1 , Protéines de fusion recombinantes/génétique , Protéines de fusion recombinantes/métabolisme , Facteurs de transcription/génétique , Protéines de Xénope , Xenopus laevis/embryologieRÉSUMÉ
The purpose of this study was to make an explicit test of the idea that a retinoid could act as a morphogen, differentially activating genes and specifying anteroposterior (a-p) level in the developing vertebrate central nervous system (CNS). Our approach was to characterize the concentration-dependent effects of retinoic acid (RA) on the neural expression of a set of a-p patterning genes, both in vivo and in an in vitro system for neural patterning. Our results indicate that a retinoid is unlikely to specify a-p level along the entire CNS. Instead, our data support the idea that the developing hindbrain may be patterned by a retinoid gradient. Sequentially more posterior hindbrain patterning genes were induced effectively by sequentially higher RA concentration windows. The most posterior CNS level induced under our RA treatment conditions corresponded to the most posterior part of the hindbrain.
Sujet(s)
Rhombencéphale/métabolisme , Trétinoïne/métabolisme , Animaux , Régulation de l'expression des gènes au cours du développement/effets des médicaments et des substances chimiques , Protéines à homéodomaine/génétique , Modèles biologiques , Rhombencéphale/effets des médicaments et des substances chimiques , Rhombencéphale/embryologie , Trétinoïne/pharmacologie , XenopusRÉSUMÉ
Oct-1, a member of the POU family of transcription factors, is expressed at relatively high levels in ectodermal and mesodermal cell lineages during early Xenopus embryogenesis (Veenstra et al, 1995). Here we show that overexpression of Oct-1 induces programmed cell death concomitant with the loss of the posterior part of the body axis. Truncated Oct-1 variants, missing either the C-terminal or N-terminal trans-activation domain, exhibit a different capacity to cause such developmental defects. Oct-1-induced cell death is rescued in unilaterally injected embryos by non-injected cells, indicative of the non-cell autonomous character of the developmental effects of Oct-1. This was confirmed by marker gene analysis, which showed a significant decrease in brachyury expression, suggesting that Oct-1 interferes with an FGF-type signalling pathway.