RÉSUMÉ
The use of manganese bacteriopheophorbide characterized by a high relaxation capacity and selectively accumulating in the tumor as a contrast agent for magnetic resonance tomography significantly improves tumor contrasting against the background of normal tissues. The pharmacokinetics and selectivity of accumulation were studied by diffuse reflection spectroscopy.
Sujet(s)
Bactériochlorophylles/composition chimique , Produits de contraste/composition chimique , Imagerie par résonance magnétique/méthodes , Composés du manganèse/composition chimique , Animaux , Bactériochlorophylles/pharmacocinétique , Carcinome d'Ehrlich/imagerie diagnostique , Carcinome d'Ehrlich/métabolisme , Carcinome d'Ehrlich/anatomopathologie , Produits de contraste/pharmacocinétique , Composés du manganèse/pharmacocinétique , Souris , Souris nude , ScintigraphieRÉSUMÉ
The subject of the paper is study of optical absorption of sensitizers in biological tissue. The study shows that absorbance can be used as a tool that allows studying biodistribution of sensitizers and their interaction with tissue in vivo. The article presents a simple technique of determining biological tissue absorption in vivo, and discusses the results of experimental animal studies of some sensitizers.
Sujet(s)
Modèles biologiques , Radiosensibilisants/pharmacologie , Analyse spectrale/instrumentation , Tomographie par cohérence optique/méthodes , Conception d'appareillage , Fluorescence , Humains , Imagerie par résonance magnétique , Radiosensibilisants/administration et posologieRÉSUMÉ
We studied the possibility of using liposomal forms of hydroxyaluminium tetra-3-phenylthiophthalocyanine as a near infrared band photosensitizer. Experiments on mice with solid Ehrlich tumor and subcutaneously transplanted P-388 leukemia revealed high selectivity of accumulation of the photosensitizer in tumors in comparison with normal tissues and high photodynamic activity of the preparation. This photosensitizer can be used as the basis for creating an effective preparation for photodynamic therapy and fluorescent diagnosis.
Sujet(s)
Carcinome d'Ehrlich/traitement médicamenteux , Indoles/usage thérapeutique , Leucémie P388/traitement médicamenteux , Composés organométalliques/usage thérapeutique , Photothérapie dynamique , Photosensibilisants/usage thérapeutique , Animaux , Carcinome d'Ehrlich/diagnostic , Leucémie P388/diagnostic , Souris , Transplantation tumoraleRÉSUMÉ
Conjugates of cobalt and aluminum phthalocyanines with monoclonal antibodies were synthesized using an AOT/n-octane reversed micellar system or water-organic mixtures with a low content of organic solvent as media. The effect of the degree of hydration of the micelles and the concentration of phthalocyanines on the composition of conjugates was studied. The immune activity of the resulting conjugates in comparison to that of native antibodies was evaluated. The catalytic activity of free cobalt phthalocyanines and their antibody conjugates was studied in the reaction of ascorbic acid oxidation.
