Colonoscopic Ultrasound-Guided Fine-Needle Aspiration Using a Curvilinear Array Transducer: A Single-Center Retrospective Cohort Study.

BACKGROUND: Curvilinear array ultrasound transducers enable tissue sampling and have therapeutic capabilities. Nevertheless, colonic intubation and maneuvering with these transducers is technically challenging and is therefore typically limited to the rectosigmoid area. This retrospective cohort study aimed to evaluate the safety, feasibility, and diagnostic yield of colonoscopic ultrasound-guided fine-needle aspiration in deep colonic intubation. IMPACT OF INNOVATION: The impact of this innovation is to enable tissue sampling of colonic and extracolonic lesions guided by endoscopic ultrasound. TECHNOLOGY, MATERIALS, AND METHODS: Curvilinear array ultrasound is used in the evaluation of luminal and extraluminal colonic diseases. Thirteen patients underwent colonoscopic ultrasound with a curvilinear array ultrasound endoscope in a single center for subepithelial lesions, cancer staging, and extracolonic lesions from July 2015 to February 2021. Endosonography was performed using an Olympus EU-ME1 and GF-UCT 180 with a 5-12MHz curvilinear array transducer. The primary outcome was the percentage of patients who were successfully scanned with the endoscopic ultrasound. The secondary outcomes included the success rate of fine-needle aspiration, the diagnostic yield of the tissue samples, and the adverse events related to the procedure. PRELIMINARY RESULTS: A total of 13 female patients underwent colonoscopic ultrasound. All patients (100%, 13/13) were successfully scanned. Fine-needle aspiration was deemed necessary and successfully performed in 100% (5/5) of the patients. Tissue samples collected by fine-needle aspiration resulted in a diagnostic yield of 60%, and no adverse events resulted from this intervention. CONCLUSIONS: This study demonstrates the feasibility of performing colonoscopic ultrasound with a curvilinear array transducer. Fine-needle aspiration for subepithelial, colonic, and extracolonic lesions is feasible and safe in this setting with no adverse events reported in our study. FUTURE DIRECTIONS: Future research should be directed toward validating colonoscopic ultrasound with a curvilinear array transducer technique in prospective randomized trials. Studies evaluating the feasibility and safety of endoscopic ultrasound-guided interventions in the colon, such as abscess drainage and enteral anastomosis, should be considered.

The contribution of selected organic substrates to the anaerobic cometabolism of sulfamethazine.

Biodegradation of organic micropollutants is likely to occur due to cometabolism by particular microbial groups. In an effort to identify the stages of anaerobic digestion potentially involved in the biodegradation of the veterinary antimicrobial sulfamethazine (SMZ), the influence of selected carbon sources (sucrose, glucose, fructose, ethanol, meat extract, cellulose, soluble starch, soy oil, acetic acid, propionic acid and butyric acid) on SMZ removal by anaerobic sludge was evaluated in short-term batch experiments. Adsorption to the granular sludge constituted a significant removal mechanism, accounting for 39% of SMZ removal in control experiments. The presence of glucose, fructose, sucrose and meat extract exerted an inducing effect on SMZ degradation, resulting in removal efficiencies of 54, 53, 58 and 61%, respectively, indicating the occurrence of cometabolism. Time courses of sucrose and meat extract degradation revealed markedly distinct organic acid profiles but resulted in similar SMZ removals. Temporal profiles of acetic and propionic acid degradation were not associated with SMZ removal, as changes in SMZ concentration were observed even after the organic acids had been completely removed. The experimental results suggest that SMZ cometabolism is not associated to sucrose hydrolysis, acetoclastic methanogenesis and acetogenesis from propionic acid.

Efficacy of root surface biomodification in root coverage: a systematic review.

BACKGROUND: Although many Authors have reported the use of root surface biomodification (RSB), the clinical outcomes in terms of root coverage are still unclear, as a number of biomodifier agents have been used in combination with various periodontal plastic surgical techniques. In this review we aim to evaluate the efficacy of RSB in root coverage and its impact on the outcomes. METHODS: A search of MEDLINE and Cochrane databases was carried out along with 2 manual searches. A combination of specific terms was used to identify relevant studies that met well-defined inclusion and exclusion criteria. Clinical attachment level, probing depth and percentage root coverage after treatment were among the results expected to be reported in the included articles. RESULTS: A total of 10 articles were identified and data were extracted. Only 6 met the inclusion criteria. Among these 6 studies, citric acid, ethylenediaminetetraacetic acid and laser therapy were used as RSB agents and free gingival graft, subepithelial connective tissue graft plus coronally advanced flap and semilunar coronally respositioned flap were the surgical approaches to root coverage treatment. Baseline and outcome data were analyzed and compared. CONCLUSION: RSB provided no additional benefit in terms of the evaluated clinical parameters. Within the limitations of this review, we found no evidence to support the use of RSB prior to root coverage treatment.

Retalho posicionado lateralmente e enxerto de tecido conjuntivo subeptelial no tratamento das recessões teciduais marginais / Laterally positioned flap and subepithelial connective tissue graft for marginal tissue recession treatment

Introdução: As cirurgias mucogengivais têm sido amplamente utilizadas para o tratamento de problemas periodontais. Nos casos de recobrimento radicular diversas são as técnicas utilizadas, dentre elas, o retalho posicionado lateralmente (RPL) e o enxerto de tecido conjuntivo subepitelial (ETCS). Objetivo: Apresentar um caso clínico de recobrimento radicular no qual foram utilizadas conjuntamente as técnicas de RPL e ETCS. Relato: Paciente procurou tratamento periodontal com queixa principal de sensibilidade dental. Foram observadas recessões classe I de Miller nos dentes 23, 24 e 25 e faixa de tecido queratinizado de 1 mm na região dos dentes em questão. A técnica escolhida para tratamento das recessões foi o enxerto de tecido conjuntivo associado ao retalho posicionado lateralmente. Resultados: Após 35 dias observou-se completa cobertura da superfície radicular e ganho de tecido queratinizado de 4 a 5 mm.

Introduction: Mucogingival surgeries have been widely used for the treatment of periodontal problems. In cases of root coverage many techniques are used, among them, the laterally positioned flap (LPF) and subepithelial connective tissue graft (SCTG). Objective: To present a clinical case of root coverage in which was used together the techniques of LPF and SCTG. Report: The patient sought for periodontal treatment complaining of tooth sensitivity. It was observed Miller Class I recessions on teeth 23, 24 and 25 and 1 mm of keratinized tissue track in the region of these teeth. The technique used for treatment of recessions was the connective tissue graft associated with the laterally positioned flap. Results: After 35 days was observed complete coverage of the root surface and keratinized tissue gain 4 to 5 mm.